1. GZMKhigh CD8+ T effector memory cells are associated with CD15high neutrophil abundance in non-metastatic colorectal tumors and predict poor clinical outcome
- Author
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Silvia Tiberti, Carlotta Catozzi, Ottavio Croci, Mattia Ballerini, Danilo Cagnina, Chiara Soriani, Caterina Scirgolea, Zheng Gong, Jiatai He, Angeli D. Macandog, Amir Nabinejad, Carina B. Nava Lauson, Arianna Quinte’, Giovanni Bertalot, Wanda L. Petz, Simona P. Ravenda, Valerio Licursi, Paola Paci, Marco Rasponi, Luca Rotta, Nicola Fazio, Guangwen Ren, Uberto Fumagalli-Romario, Martin H. Schaefer, Stefano Campaner, Enrico Lugli, Luigi Nezi, and Teresa Manzo
- Subjects
Multidisciplinary ,Neutrophils ,Humans ,General Physics and Astronomy ,Lymphocytes, Tumor-Infiltrating ,CD8-Positive T-Lymphocytes ,Colorectal Neoplasms ,Tumor-Infiltrating ,Lymphocytes ,General Chemistry ,General Biochemistry, Genetics and Molecular Biology - Abstract
CD8+T cells are a major prognostic determinant in solid tumors, including colorectal cancer (CRC). However, understanding how the interplay between different immune cells impacts on clinical outcome is still in its infancy. Here, we describe that the interaction of tumor infiltrating neutrophils expressing high levels of CD15 with CD8+T effector memory cells (TEM) correlates with tumor progression. Mechanistically, stromal cell-derived factor-1 (CXCL12/SDF-1) promotes the retention of neutrophils within tumors, increasing the crosstalk with CD8+T cells. As a consequence of the contact-mediated interaction with neutrophils, CD8+T cells are skewed to produce high levels of GZMK, which in turn decreases E-cadherin on the intestinal epithelium and favors tumor progression. Overall, our results highlight the emergence of GZMKhighCD8+TEMin non-metastatic CRC tumors as a hallmark driven by the interaction with neutrophils, which could implement current patient stratification and be targeted by novel therapeutics.
- Published
- 2022