1. Targeted Quantitative Kinome Analysis Identifies PRPS2 as a Promoter for Colorectal Cancer Metastasis.
- Author
-
Wang, Yinsheng and Miao, Weili
- Subjects
Myc ,PRPS2 ,SILAC ,colorectal cancer ,kinase ,kinome ,matrix metalloproteinase ,metastasis ,parallel-reaction monitoring ,quantitative proteomics ,Antigens ,CD ,Cadherins ,Cell Line ,Tumor ,Cell Movement ,Colorectal Neoplasms ,Gene Expression Profiling ,Gene Expression Regulation ,Neoplastic ,Humans ,Lymphatic Metastasis ,Matrix Metalloproteinase 9 ,Neoplasm Invasiveness ,Protein Kinases ,Proto-Oncogene Mas ,Proto-Oncogene Proteins c-myc ,RNA ,Small Interfering ,Ribose-Phosphate Pyrophosphokinase ,Tumor Cells ,Cultured - Abstract
Kinases are among the most important families of enzymes involved in cell signaling. In this study, we employed a recently developed parallel-reaction monitoring (PRM)-based targeted proteomic method to examine the reprogramming of the human kinome during colorectal cancer (CRC) metastasis. We were able to quantify the relative expression of 299 kinase proteins in a pair of matched primary/metastatic CRC cell lines. We also found that, among the differentially expressed kinases, phosphoribosyl pyrophosphate synthetase 2 (PRPS2) promotes the migration and invasion of cultured CRC cells through regulating the activity of matrix metalloproteinase 9 (MMP-9) and the expression of E-cadherin. Moreover, we found that the up-regulation of PRPS2 in metastatic CRC cells could be induced by the MYC proto-oncogene. Together, our unbiased kinome profiling approach led to the identification, for the first time, of PRPS2 as a promoter for CRC metastasis.
- Published
- 2019