1. KH-type splicing regulatory protein is a new component of chromatoid body
- Author
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Lin Liu, Fei Sun, Huijuan Zhang, Xiaolin Liang, Mo-Fang Liu, Yu Lin, Chu-Fang Chou, Guishuan Wang, He-Feng Huang, and Yi-Yu Lin
- Subjects
Adult ,Male ,0301 basic medicine ,Embryology ,RNA-binding protein ,Biology ,Poly(A)-Binding Protein I ,Poly(A)-Binding Protein II ,DEAD-box RNA Helicases ,Young Adult ,03 medical and health sciences ,Endocrinology ,Gene expression ,Animals ,Humans ,RNA, Messenger ,Nuclear protein ,Spermatogenesis ,Cells, Cultured ,Heat-Shock Proteins ,Mice, Knockout ,Regulation of gene expression ,Gene Expression Regulation, Developmental ,Nuclear Proteins ,RNA-Binding Proteins ,Obstetrics and Gynecology ,Oligospermia ,Cell Biology ,Argonaute ,Spermatids ,Cell biology ,DNA-Binding Proteins ,MicroRNAs ,030104 developmental biology ,Reproductive Medicine ,Case-Control Studies ,Argonaute Proteins ,RNA splicing ,Trans-Activators ,Chromatoid body ,Protein Binding ,Signal Transduction - Abstract
The chromatoid body (CB) is a specific cloud-like structure in the cytoplasm of haploid spermatids. Recent findings indicate that CB is identified as a male germ cell-specific RNA storage and processing center, but its function has remained elusive for decades. In somatic cells, KH-type splicing regulatory protein (KSRP) is involved in regulating gene expression and maturation of select microRNAs (miRNAs). However, the function of KSRP in spermatogenesis remains unclear. In this study, we showed that KSRP partly localizes in CB, as a component of CB. KSRP interacts with proteins (mouse VASA homolog (MVH), polyadenylate-binding protein 1 (PABP1) and polyadenylate-binding protein 2 (PABP2)), mRNAs (Tnp2 and Odf1) and microRNAs (microRNA-182) in mouse CB. Moreover, KSRP may regulate the integrity of CB via DDX5-miRNA-182 pathway. In addition, we found abnormal expressions of CB component in testes of Ksrp-knockout mice and of patients with hypospermatogenesis. Thus, our results provide mechanistic insight into the role of KSRP in spermatogenesis.
- Published
- 2017