Author: "Nestlé Institute of Health Sciences SA [Lausanne, Switzerland]" / Topic: humans - Searchworks@Jio Institute Digital Library Search Results

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1. Effect of an enteral amino acid blend on muscle and gut functionality in critically ill patients: a proof-of-concept randomized controlled trial

Author: Heming, Nicholas, Carlier, Robert Yves, Prigent, Hélène, Mekki, Ahmed, Jousset, Camille, Lofaso, Frédéric, Ambrosi, Xavier, Bounab, Rania, Maxime, Virginie, Mansart, Arnaud, Crenn, P., Moine, Pierre, Foltzer, Fabien, Cuenoud, Bernard, Konz, Tobias, Corthesy, John, Beaumont, Maurice, Hartweg, Mickaël, Roessle, Claudia, Preiser, Jean Charles E., Breuillé, Denis, Annane, Djillali, HAL UVSQ, Équipe, Infection et inflammation (2I), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital Raymond Poincaré [Garches], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), AP-HP. Université Paris Saclay, Université de Versailles Saint-Quentin-en-Yvelines - UFR Sciences de la santé Simone Veil (UVSQ Santé), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Centre hospitalier universitaire de Nantes (CHU Nantes), Nestlé Research Center | Centre de recherche Nestlé [Lausanne], Nestlé S.A., Nestlé Institute of Health Sciences SA [Lausanne, Switzerland], Université libre de Bruxelles (ULB), Nestlé Health Science, and This study was funded by Nestlé Health Science. The experimental product, placebo and feed were all provided by Nestlé Health Science. Nestlé employees were involved in designing the study and in writing the manuscript.
Subjects: [SDV]Life Sciences [q-bio], Critical Illness, Muscles, Alanine Transaminase, Critical Care and Intensive Care Medicine, Alkaline Phosphatase, [SDV] Life Sciences [q-bio], Protein balance, Sepsis, Creatinine, Humans, Citrulline, Intensive care unit, Nutrition
Abstract: Background A defining feature of prolonged critical illness is muscle wasting, leading to impaired recovery. Supplementation with a tailored blend of amino acids may bolster the innate gut defence, promote intestinal mucosa repair and limit muscle loss. Methods This was a monocentric, randomized, double-blind, placebo-controlled study that included patients with sepsis or acute respiratory distress syndrome. Patients received a specific combination of five amino acids or placebo mixed with enteral feeding for 21 days. Markers of renal function, gut barrier structure and functionality were collected at baseline and 1, 2, 3 and 8 weeks after randomization. Muscle structure and function were assessed through MRI measurements of the anterior quadriceps volume and by twitch airway pressure. Data were compared between groups relative to the baseline. Results Thirty-five critically ill patients were randomized. The amino acid blend did not impair urine output, blood creatinine levels or creatinine clearance. Plasma citrulline levels increased significantly along the treatment period in the amino acid group (difference in means [95% CI] 5.86 [1.72; 10.00] nmol/mL P = 0.007). Alanine aminotransferase and alkaline phosphatase concentrations were lower in the amino acid group than in the placebo group at one week (ratio of means 0.5 [0.29; 0.86] (P = 0.015) and 0.73 [0.57; 0.94] (P = 0.015), respectively). Twitch airway pressure and volume of the anterior quadriceps were greater in the amino acid group than in the placebo group 3 weeks after randomization (difference in means 10.6 [0.99; 20.20] cmH20 (P = 0.035) and 3.12 [0.5; 5.73] cm3/kg (P = 0.022), respectively). Conclusions Amino acid supplementation increased plasma citrulline levels, reduced alanine aminotransferase and alkaline phosphatase levels, and improved twitch airway pressure and anterior quadriceps volume. Trial registration ClinicalTrials.gov, NCT02968836. Registered November 21, 2016.
Published: 2022
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2. OBEDIS Core Variables Project: European Expert Guidelines on a Minimal Core Set of Variables to Include in Randomized, Controlled Clinical Trials of Obesity Interventions

Author: António L. Palmeira, Thorkild I. A. Sørensen, Martin Neovius, Martine Laville, Mikael Rydén, Andrea Natali, Maud Alligier, Romain Barrès, Kristina Campbell, David Jacobi, I. Sadaf Farooqi, Jean-Michel Oppert, Helen M. Roche, André Scheen, Karine Clément, Ellen E. Blaak, Yves Boirie, Gijs H. Goossens, Jason C.G. Halford, Luc Tappy, Nathalie Farpour-Lambert, Hannele Yki-Järvinen, Uberto Pagotto, Chantal Simon, Jörg Hager, Jildau Bouwman, Paul Brunault, Gema Frühbeck, Dominique Langin, Olivier Ziegler, Chantal Julia, Hans Hauner, Centre de Recherche en Nutrition Humaine Rhône-Alpes (CRNH-RA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-CHU Grenoble-Hospices Civils de Lyon (HCL)-CHU Saint-Etienne-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA), University of Copenhagen = Københavns Universitet (KU), Novo Nordisk Foundation Center for Basic Metabolic Research (CBMR), Faculty of Health and Medical Sciences, University of Copenhagen = Københavns Universitet (KU)-University of Copenhagen = Københavns Universitet (KU), Maastricht University Medical Centre (MUMC), Maastricht University [Maastricht], CHU Clermont-Ferrand, Netherlands Organization for Applied Scientific Research (TNO), TNO Science and Industry, Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Tours (UT), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Imagerie et cerveau (iBrain - Inserm U1253 - UNIV Tours ), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), KC Microbiome Communications Group, Nutrition et obésités: approches systémiques (nutriomics) (UMR-S 1269 INSERM - Sorbonne Université), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), NIHR Biomedical Research Centre [London], Guy's and St Thomas' NHS Foundation Trust-King‘s College London, University of Cambridge [UK] (CAM), Université de Genève (UNIGE), Geneva University Hospitals and Geneva University, Clínica Universidad de Navarra [Pamplona], Nestlé Institute of Health Sciences SA [Lausanne, Switzerland], University of Liverpool, Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM), Klinikums rechts der Isar, Centre National de la Recherche Scientifique (CNRS), Université de Nantes (UN), unité de recherche de l'institut du thorax UMR1087 UMR6291 (ITX), Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Equipe 3: EREN- Equipe de Recherche en Epidémiologie Nutritionnelle (CRESS - U1153), Université Sorbonne Paris Nord-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées, Institut des Maladies Métaboliques et Cardiovasculaires (I2MC), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), University of Pisa - Università di Pisa, Karolinska Institutet [Stockholm], CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Institute of cardiometabolism and nutrition (ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), Alma Mater Studiorum University of Bologna (UNIBO), Université de Lisbonne, University College Dublin [Dublin] (UCD), Centre Hospitalier Universitaire de Liège (CHU-Liège), Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), Université de Lausanne (UNIL), Minerva Foundation Institute for Medical Research, University of Helsinki, Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Joint Programming Initiative Heathy Diet for Healthy Life, Alligier M., Barres R., Blaak E.E., Boirie Y., Bouwman J., Brunault P., Campbell K., Clement K., Farooqi I.S., Farpour-Lambert N.J., Fruhbeck G., Goossens G.H., Hager J., Halford J.C.G., Hauner H., Jacobi D., Julia C., Langin D., Natali A., Neovius M., Oppert J.M., Pagotto U., Palmeira A.L., Roche H., Ryden M., Scheen A.J., Simon C., Sorensen T.I.A., Tappy L., Yki-Jarvinen H., Ziegler O., Laville M., FCRIN/FORCE Network, Centre de Recherche en Nutrition Humaine Rhône-Alpes, Novo Nordisk, Department of Human Biology, Maastricht University Medical Center (MUMC), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Nutrition clinique, Centre Hospitalier Universitaire Gabriel Montpied, TNO,Netherlands Organisation for Applied Scientific Research, UMR U 1253, Université de Tours, Nutrition et obésité : approches systémiques, Sorbonne Université, Wellcome-MRC Institute of Metabolic Science and NIHR Biomedical Research Centre, University of Cambridge, Service of Therapeutic Education for Chronic Diseases, Department of Community Health, Primary Care and Emergency, Geneva University Hospitals, Department of Endocrinology and Nutrition, Navarra Public Health Institute, Centro de Investigación Biomédica en Red de la Fisiopatología de la Obesidad y Nutrición - Biomedical Research Center in Red-Physiopathology of Obesity and Nutrition (CIBEROBN), University of Navarra, University of Cape Town, Metabolic Phenotyping, Nestlé Institute of Health Sciences, Psychological Sciences, University of Liverpool (University of Liverpool), Institut für Ernährungsmedizin des Klinikums Rechts der Isar, Technical University of Munich (TUM), Institut du Thorax, Centre Hospitalier Universitaire de Nantes, Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC), Université Toulouse III - Paul Sabatier, Centre Hospitalier Universitaire de Toulouse, Department of Clinical and Experimental Medicine, Università degli studi di Napoli Federico II, Department of Medicine (Solna), Nutrition, Institut de Cardiométabolisme et Nutrition (ICAN), CHU Pitié-Salpêtrière [APHP], Department of Medical and Surgical Sciences, Universita degli Studi di Padova, CIPER, PANO-SR, Faculty of Human Kinetics, University of Lisbon, UCD Institute of Food and Health, University College Dublin (UCD), Department of Medicine, The University of Sydney, Diabètes, Nutrition et maladies métaboliques, Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), Physiologie, Université de Picardie Jules Verne (UPJV), Helsinki University Central Hospital, Endocrinologie, Diabetes et Nutrition, Hôpital Brabois, Maastricht University [Maastricht]-Maastricht University [Maastricht], Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Sorbonne Université-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, ProdInra, Archive Ouverte, Université de Lyon-Université de Lyon-CHU Grenoble-Hospices Civils de Lyon (HCL)-CHU Saint-Etienne-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA), University of Copenhagen = Københavns Universitet (UCPH), University of Copenhagen = Københavns Universitet (UCPH)-University of Copenhagen = Københavns Universitet (UCPH), Nutrition et obésités: approches systémiques (UMR-S 1269) (Nutriomics), Université de Genève = University of Geneva (UNIGE), Unité de recherche de l'institut du thorax (ITX-lab), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Research Unit on Cardiovascular and Metabolic Diseases (ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut de Cardiométabolisme et Nutrition = Institute of Cardiometabolism and Nutrition [CHU Pitié Salpêtrière] (IHU ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-CHU Pitié-Salpêtrière [AP-HP], Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Lausanne = University of Lausanne (UNIL), Helsingin yliopisto = Helsingfors universitet = University of Helsinki, HUS Internal Medicine and Rehabilitation, Humane Biologie, and RS: NUTRIM - R1 - Obesity, diabetes and cardiovascular health
Subjects: 0301 basic medicine, Research design, Gerontology, Health (social science), Psychological intervention, Choice Behavior, 0302 clinical medicine, QUALITY-OF-LIFE, OBSTRUCTIVE SLEEP-APNEA, Medicine, Medical History Taking, lcsh:RC620-627, Interventions, METABOLIC SYNDROME, Randomized Controlled Trials as Topic, ddc:616, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Precision medicine, Prognosis, C-REACTIVE PROTEIN, 3. Good health, Europe, lcsh:Nutritional diseases. Deficiency diseases, CARDIOVASCULAR-DISEASE, Research Design, Variable, lcsh:Nutrition. Foods and food supply, Human, Prognosi, education, WEIGHT-LOSS, lcsh:TX341-641, Intervention, 030209 endocrinology & metabolism, GASTRIC BYPASS-SURGERY, Guidelines, Patient care, 03 medical and health sciences, Variables, Physiology (medical), Humans, CORONARY-HEART-DISEASE, Obesity, Expert Testimony, Core set, 030109 nutrition & dietetics, business.industry, Stratification, medicine.disease, Diet, BODY-MASS INDEX, Clinical trial, PHYSICAL-ACTIVITY, Biological Variation, Population, 3121 General medicine, internal medicine and other clinical medicine, Metabolic syndrome, business, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: Heterogeneity of interindividual and intraindividual responses to interventions is often observed in randomized, controlled trials for obesity. To address the global epidemic of obesity and move toward more personalized treatment regimens, the global research community must come together to identify factors that may drive these heterogeneous responses to interventions. This project, called OBEDIS (OBEsity Diverse Interventions Sharing - focusing on dietary and other interventions), provides a set of European guidelines for a minimal set of variables to include in future clinical trials on obesity, regardless of the specific endpoints. Broad adoption of these guidelines will enable researchers to harmonize and merge data from multiple intervention studies, allowing stratification of patients according to precise phenotyping criteria which are measured using standardized methods. In this way, studies across Europe may be pooled for better prediction of individuals' responses to an intervention for obesity - ultimately leading to better patient care and improved obesity outcomes. © 2020 The Author(s) Published by S. Karger AG, Basel.
Published: 2020
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3. The Dietary Inflammatory Index Is Associated with Subclinical Mastitis in Lactating European Women

Author: Myriam C. Afeiche, Alison Iroz, Frank Thielecke, Antonio C. De Castro, Gregory Lefebvre, Colleen F. Draper, Cecilia Martínez-Costa, Kirsti Haaland, Giovanna Marchini, Massimo Agosti, Magnus Domellöf, Thameur Rakza, Maria José Costeira, Mireille Vanpee, Claude Billeaud, Jean-Charles Picaud, Daryl Lim Kah Hian, Guimei Liu, Nitin Shivappa, James R. Hébert, Tinu M. Samuel, Nestlé Institute of Health Sciences SA [Lausanne, Switzerland], Swiss Distance University of Applied Sciences [Regensdorf-Zurich, Switzerland] (SDUAS), SAS Institute Pte Ltd. [Singapore], Crown Bioscience [San Diego, CA, USA] (CB), EPFL Innovation Park [Lausanne, Switzerland], Universitat de València (UV), Oslo University Hospital [Oslo], Karolinska University Hospital [Stockholm], Ospedale del Ponte [Varese, Italy], Universitá degli Studi dell’Insubria = University of Insubria [Varese] (Uninsubria), Umeå University, Centre d'Investigation Clinique - Innovation Technologique de Lille - CIC 1403 - CIC 9301 (CIC Lille), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Instituto de Investigação em Ciências da Vida e Saúde [Braga, Portugal] (Escola de Ciências da Saúde), Universidade do Minho = University of Minho [Braga], CHU de Bordeaux Pellegrin [Bordeaux], Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Hôpital de la Croix-Rousse [CHU - HCL], Hospices Civils de Lyon (HCL), Institute for Infocomm Research - I²R [Singapore], University of South Carolina [Columbia], and CarMeN, laboratoire
Subjects: Nutrition and Dietetics, Milk, Human, [SDV.BA] Life Sciences [q-bio]/Animal biology, [SDV.BA]Life Sciences [q-bio]/Animal biology, lactating women, dietary inflammatory index, subclinical mastitis, multicenter longitudinal cohort, Sodium, Mastitis, Mastitis/complications, Diet, Näringslära, Milk, Lactation, Humans, Human/chemistry, Female, Sodium/analysis, Food Science
Abstract: International audience; Subclinical mastitis (SCM) is an inflammatory state of the lactating mammary gland, which is asymptomatic and may have negative consequences for child growth. The objectives of this study were to: (1) test the association between the dietary inflammatory index (DII(®)) and SCM and (2) assess the differences in nutrient intakes between women without SCM and those with SCM. One hundred and seventy-seven women with available data on human milk (HM) sodium potassium ratio (Na:K) and dietary intake data were included for analysis. Multivariable logistic regression was used to examine the association between nutrient intake and the DII score in relation to SCM. Women without SCM had a lower median DII score (0.60) than women with moderate (1.12) or severe (1.74) SCM (p < 0.01). A one-unit increase in DII was associated with about 41% increased odds of having SCM, adjusting for country and mode of delivery, p = 0.001. Women with SCM had lower mean intakes of several anti-inflammatory nutrients. We show for the first time exploratory evidence that SCM may be associated with a pro-inflammatory diet and women with SCM have lower intakes of several antioxidant and anti-inflammatory nutrients.
Published: 2022
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4. High hydrostatic pressure processing of human milk preserves milk oligosaccharides and avoids formation of Maillard reaction products

Author: Edwina Micours, Marie de Lamballerie, Frédéric Gottrand, Delphine Ley, Jean Lesage, Sarahi Jaramillo-Ortiz, Mike Howsam, Lucie Marousez, Léa Tran, Norbert Sprenger, Frédéric J. Tessier, Institute for Translational Research in Inflammation - U 1286 (INFINITE (Ex-Liric)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Nestlé Research Center | Centre de recherche Nestlé [Lausanne], Nestlé S.A., Matrices Aliments Procédés Propriétés Structure - Sensoriel (GEPEA-MAPS2), Laboratoire de génie des procédés - environnement - agroalimentaire (GEPEA), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Nantes université - UFR des Sciences et des Techniques (Nantes univ - UFR ST), Nantes Université - pôle Sciences et technologie, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Sciences et technologie, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - Institut Universitaire de Technologie Saint-Nazaire (Nantes Univ - IUT Saint-Nazaire), Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique (IMT Atlantique), Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ), Facteurs de Risque et Déterminants Moléculaires des Maladies liées au Vieillissement - U 1167 (RID-AGE), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Hôpital Jeanne de Flandres, Université de Lille, Droit et Santé-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), ANR-18-CE21-0005,HHP-humanmilk,Le traitement du lait maternel par haute pression hydrostatique augmente ses qualités biologiques et intestinotrophiques : études expérimentales ex-vivo et chez le nouveau-né prématuré.(2018), Université de Lille, Inserm, CHU Lille, Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286, Nestlé Institute of Health Sciences SA [Lausanne, Switzerland], Laboratoire de génie des procédés - environnement - agroalimentaire [GEPEA], Facteurs de Risque et Déterminants Moléculaires des Maladies liées au Vieillissement - U 1167 [RID-AGE], Institute for Translational Research in Inflammation - U 1286 [INFINITE (Ex-Liric)], Lille Inflammation Research International Center (LIRIC) - U995, and Facteurs de risque et déterminants moléculaires des maladies liées au vieillissement (RID-AGE) - U1167
Subjects: Glycation End Products, Advanced, Food Handling, Hydrostatic pressure, Oligosaccharides, Critical Care and Intensive Care Medicine, Hop (networking), symbols.namesake, High hydrostatic pressure, Tandem Mass Spectrometry, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Hydrostatic Pressure, Medicine, Humans, Carboxyethyllysine, Food science, Holder pasteurization, Nutrition and Dietetics, Maillard reaction products, Milk, Human, business.industry, Human milk, Human milk oligosaccharides, food and beverages, Lactuloselysine, Raw milk, Mass spectrometric, Maillard reaction, symbols, business, [CHIM.OTHE]Chemical Sciences/Other, Chromatography, Liquid
Abstract: International audience; Background & aims: High hydrostatic pressure (HHP) processing is a non-thermal method proposed as an alternative to Holder pasteurization (HoP) for the treatment of human milk. HHP preserves numerous milk bioactive components that are degraded by HoP, but no data are available for milk oligosaccharides (HMOs) or the formation of Maillard reaction products, which may be deleterious for preterm newborns.Methods: We evaluated the impact of HHP processing of human milk on 22 HMOs measured by liquid chromatography with fluorescence detection and on furosine, lactuloselysine, carboxymethyllysine (CML) and carboxyethyllysine (CEL) measured by liquid chromatography with tandem mass spectrometric detection (LC-MS/MS), four established indicators of the Maillard reaction. Human raw milk was sterilized by HoP (62.5 °C for 30 min) or processed by HHP (350 MPa at 38 °C).Results: Neither HHP nor HoP processing affected the concentration of HMOs, but HoP significantly increased furosine, lactuloselysine, CML and CEL levels in milk.Conclusions: Our findings demonstrate that HPP treatment preserves HMOs and avoids formation of Maillard reaction products. Our study confirms and extends previous findings that HHP treatment of human milk provides safe milk, with fewer detrimental effects on the biochemically active milk components than HoP.
Published: 2021
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5. Network Analyses Reveal Negative Link Between Changes in Adipose Tissue GDF15 and BMI During Dietary-induced Weight Loss

Author: Aline Charpagne, Ondine Walter, Nathalie Viguerie, Julien Marquis, Jörg Hager, Nathalie Boulet, Sylviane Metairon, Julie Vion, Claire Laurens, Arne Astrup, Dominique Langin, Armand Valsesia, Nathalie Vialaneix, Alyssa Imbert, Wim H. M. Saris, Gregory Lefebvre, Cedric Moro, RS: NUTRIM - R1 - Obesity, diabetes and cardiovascular health, Humane Biologie, Institut des Maladies Métaboliques et Casdiovasculaires (UPS/Inserm U1297 - I2MC), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Unité de Mathématiques et Informatique Appliquées de Toulouse (MIAT INRAE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Lausanne, Genomic Technologies Facility, Nestlé Institute of Health Sciences SA [Lausanne, Switzerland], Franco-czech Laboratory for clinical research on obesity, Charles University [Prague] (CU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Toulouse University Hospitals, Laboratory of Clinical Biochemistry, Maastricht University Medical Centre (MUMC), Maastricht University [Maastricht], IT University of Copenhagen (ITU), Institut National de la Sante et de la Recherche Medicale (Inserm), Paul Sabatier University, Innovative Medicines Initiative Joint Undertaking115372, European CommissionFP6-513946 DiOGenes, and Vialaneix, Nathalie
Subjects: FOS: Computer and information sciences, Male, Weight loss, low calorie diet, PROMOTES, Macrophage, Endocrinology, Diabetes and Metabolism, Clinical Biochemistry, Adipose tissue, Biochemistry, Body Mass Index, Transcriptome, chemistry.chemical_compound, network analyses, Endocrinology, [STAT.AP] Statistics [stat]/Applications [stat.AP], Adipocyte, Gene expression, Faculty of Science, OSTEOPONTIN, Gene Regulatory Networks, MACROPHAGES, [STAT.AP]Statistics [stat]/Applications [stat.AP], Prognosis, DIFFERENTIATION, Adipose Tissue, Network analyses, Female, medicine.symptom, EXPRESSION, Adult, medicine.medical_specialty, Growth Differentiation Factor 15, Diet, Reducing, Calorie restriction, Context (language use), macrophage, Biology, Statistics - Applications, Internal medicine, Weight Loss, medicine, Humans, Applications (stat.AP), Obesity, Low calorie diet, Inflammation, RECEPTOR, Biochemistry (medical), Gene expression profiling, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, CYTOKINE, REDUCTION, MICE, chemistry, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, inflammation, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, sense organs, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Biomarkers, Follow-Up Studies
Abstract: Context Adipose tissue (AT) transcriptome studies provide holistic pictures of adaptation to weight and related bioclinical settings changes. Objective To implement AT gene expression profiling and investigate the link between changes in bioclinical parameters and AT gene expression during 3 steps of a 2-phase dietary intervention (DI). Methods AT transcriptome profiling was obtained from sequencing 1051 samples, corresponding to 556 distinct individuals enrolled in a weight loss intervention (8-week low-calorie diet (LCD) at 800 kcal/day) followed with a 6-month ad libitum randomized DI. Transcriptome profiles obtained with QuantSeq sequencing were benchmarked against Illumina RNAseq. Reverse transcription quantitative polymerase chain reaction was used to further confirm associations. Cell specificity was assessed using freshly isolated cells and THP-1 cell line. Results During LCD, 5 modules were found, of which 3 included at least 1 bioclinical variable. Change in body mass index (BMI) connected with changes in mRNA level of genes with inflammatory response signature. In this module, change in BMI was negatively associated with changes in expression of genes encoding secreted protein (GDF15, CCL3, and SPP1). Through all phases of the DI, change in GDF15 was connected to changes in SPP1, CCL3, LIPA and CD68. Further characterization showed that these genes were specific to macrophages (with LIPA, CD68 and GDF15 expressed in anti-inflammatory macrophages) and GDF15 also expressed in preadipocytes. Conclusion Network analyses identified a novel AT feature with GDF15 upregulated with calorie restriction induced weight loss, concomitantly to macrophage markers. In AT, GDF15 was expressed in preadipocytes and macrophages where it was a hallmark of anti-inflammatory cells.
Published: 2021
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6. Dietary sialylated oligosaccharides in early-life may promote cognitive flexibility during development in context of obesogenic dietary intake

Author: Caroline Clouard, Stephen A. Fleming, T. Schuurman, Sietse Jan Koopmans, Jonas Hauser, I. Reimert, Wageningen University and Research [Wageningen] (WUR), Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage [Rennes] (PEGASE), AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Traverse Science, Inc., Wageningen Livestock Research, and Nestlé Institute of Health Sciences SA [Lausanne, Switzerland]
Subjects: cognition, Animal Nutrition, Swine, medicine.medical_treatment, [SDV]Life Sciences [q-bio], Oligosaccharides, Medicine (miscellaneous), Prebiotic, Ecological systems theory, memory, Eating, chemistry.chemical_compound, 0302 clinical medicine, Cognition, sialyllatose, media_common, 2. Zero hunger, 0303 health sciences, Nutrition and Dietetics, [SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior, General Neuroscience, Dietary intake, Cognitive flexibility, General Medicine, Sialyllatose, Diervoeding, Early life, prebiotic, Swine, Miniature, Adaptation Physiology, Female, media_common.quotation_subject, Biology, Development, 03 medical and health sciences, 2'-Fucosyllactose, Memory, medicine, Animals, Humans, Human milk oligosaccharide, Adaptatiefysiologie, development, 030304 developmental biology, Milk, Human, Diet, chemistry, Evolutionary biology, WIAS, human activities, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, 030217 neurology & neurosurgery, 2′-fucosyllactose, Diversity (politics)
Abstract: International audience; Introduction: Oligosaccharides found in mammalian milk have shown the potential to alter brain development across multiple species. The diversity and concentration of these oligosaccharides is species-specific and varies greatly between individuals, thus understanding their role in cognitive development is warranted. We investigated the impact of early life dietary fucosylated/neutral or sialylated human milk oligosaccharides (HMO) on behaviours in tasks assessing anxiety, motivation, appetite, learning, and memory.Methods: Sixty-four female Göttingen minipigs were artificially reared from 2 weeks postnatal and provided milk replacers. The study used four groups: no additional oligosaccharides (Con), fucosylated and neutral oligosaccharides (FN, 4 g/L), sialylated oligosaccharides (SL, 0.68 g/L), or both FN and SL (FN + SL, 4 g/L) from 2 to 11 weeks postnatal. One reference group was sow-reared. Weaning occurred between 10 and 11 weeks postnatal, and thereafter an obesogenic diet was provided. Behavioral tasks were conducted over three periods: 1) 0–11 weeks; 2) 16–29 weeks; 3) 39–45 weeks. Tasks included a spatial holeboard task, open field task, exposure to a novel object, runway task, single-feed task, and home pen behaviour observation.Results: In the holeboard, the SL group demonstrated improved reference memory during reversal trials between 16-29 weeks. All groups demonstrated equivalent behavior in open field, novel object, runway, and single-feed tasks, as well as in their home pens (Ps > 0.05).Discussion: These results suggest that early life dietary intake of sialylated oligosaccharides may provide an improvement to cognition during the equivalent developmental stage of adolescence.
Published: 2021
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7. Analysis of dietary patterns and nutritional adequacy in lactating women : a multicentre European cohort (ATLAS study)

Author: Magnus Domellöf, Mathilde Fleith, Kirsti Haaland, Massimo Agosti, Giovanna Marchini, Claude Billeaud, Frank Thielecke, Jean-Charles Picaud, Dantong Wang, Tinu Mary Samuel, Carlos Antonio De Castro, Maria Jose Costeira, Cecilia Martínez-Costa, Myriam C. Afeiche, Mireille Vanapee, Nestlé Institute of Health Sciences SA [Lausanne, Switzerland], Swiss Distance University of Applied Sciences [Regensdorf-Zurich, Switzerland] (SDUAS), T2 Bene Ltd [Allschwil, Switzerland], Universitat de València (UV), Oslo University Hospital [Oslo], Karolinska University Hospital [Stockholm], Ospedale del Ponte [Varese, Italy], Umeå University, Instituto de Investigação em Ciências da Vida e Saúde [Braga, Portugal] (Escola de Ciências da Saúde), Universidade do Minho, CHU de Bordeaux Pellegrin [Bordeaux], Karolinska Institutet [Stockholm], Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Hôpital de la Croix-Rousse [CHU - HCL], Hospices Civils de Lyon (HCL), Universidade do Minho = University of Minho [Braga], and CarMeN, laboratoire
Subjects: Vitamin, 030309 nutrition & dietetics, [SDV]Life Sciences [q-bio], Endocrinology, Diabetes and Metabolism, Nutritional Status, 030209 endocrinology & metabolism, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Cluster analysis, Multicentre European cohort, Environmental health, Pantothenic acid, Vitamin D and neurology, Medicine, Humans, Lactation, Longitudinal Studies, Dietary Reference Values, 2. Zero hunger, 0303 health sciences, Nutrition and Dietetics, Vitamin C, Milk, Human, Nutrition adequacy, business.industry, Dietary Surveys and Nutritional Epidemiology, Infant, Food Patterns, Diet, [SDV] Life Sciences [q-bio], Europe, Näringslära, Breast Feeding, chemistry, Dietary Reference Intake, Cohort, Lactating mothers, Female, business, Food Science, Research Article
Abstract: Eating habits of lactating women can influence the nutrient composition of human milk, which in turn influences nutrient intake of breastfed infants. The aim of the present study was to identify food patterns and nutritional adequacy among lactating women in Europe. Data from a multicentre European longitudinal cohort (ATLAS study) were analysed to identify dietary patterns using cluster analysis. Dietary information from 180 lactating women was obtained using 3-d food diaries over the first 4 months of lactation. Four dietary patterns were identified: ‘vege-oils’, ‘fish-poultry’, ‘confectionery-salads’ and ‘mixed dishes’. Nutrition adequacy was not significantly different between clusters, but the ‘vege-oils’ cluster tended to yield the highest nutrition adequacy measured by Mean Adequacy Ratio. Compared with European dietary reference values (DRVs) for lactating women, women in all clusters had inadequate intakes of energy, pantothenic acid, folate, vitamin C, vitamin A, vitamin D, zinc, iodine, potassium and linoleic acid. Adequate intake for fibre and α-linolenic acid was only achieved in the ‘vege-oils’ cluster. Overall, fat intake was above DRVs. The present study showed that various dietary patterns do not adequately supply all nutrients, indicating a need to promote overall healthy dietary habits for European lactating women.
Published: 2021
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8. Integrative phenotyping of glycemic responders upon clinical weight loss using multi-omics

Author: Ellen E. Blaak, Anirikh Chakrabarti, Mojgan Masoodi, Nathalie Viguerie, Armand Valsesia, Jörg Hager, Dominique Langin, Arne Astrup, Wim H. M. Saris, Nestlé Institute of Health Sciences SA [Lausanne, Switzerland], Institut des Maladies Métaboliques et Cardiovasculaires (I2MC), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Fédérale Toulouse Midi-Pyrénées, Laboratory of Clinical Biochemistry [Toulouse], CHU Toulouse [Toulouse], Maastricht University Medical Centre (MUMC), Maastricht University [Maastricht], University of Copenhagen = Københavns Universitet (KU), Bern University Hospital [Berne] (Inselspital), The study was founded by the European Commission, Food Quality and Safety Priority of the Sixth Framework Program (FP6-2005-513946), and Nestlé Institute of Health Sciences., Laboratoire de Biochimie [CHU Toulouse], Institut Fédératif de Biologie (IFB), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Pôle Biologie [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), University of Copenhagen = Københavns Universitet (UCPH), Bodescot, Myriam, RS: NUTRIM - R1 - Obesity, diabetes and cardiovascular health, and Humane Biologie
Subjects: 0301 basic medicine, Proteomics, Metabolic disorders, PROTEIN, Physiology, Adipose tissue, Ketone Bodies, CALORIC RESTRICTION, 0302 clinical medicine, Endocrinology, Weight loss, Medicine, 610 Medicine & health, INSULIN-RESISTANCE, Multidisciplinary, Area under the curve, Endocrine system and metabolic diseases, Genomics, Lipids, ADIPOSE-TISSUE, Phenotype, Cardiovascular diseases, Adipose Tissue, VISCERAL FAT, OBESITY, Area Under Curve, Body Composition, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], medicine.symptom, Diet, Reducing, Fatty Acid Elongases, Science, Down-Regulation, 030209 endocrinology & metabolism, Intra-Abdominal Fat, APOLIPOPROTEIN-E, Article, 03 medical and health sciences, Insulin resistance, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Weight Loss, Humans, DE-NOVO LIPOGENESIS, Glycemic, business.industry, medicine.disease, Omics, Obesity, Confidence interval, Computational biology and bioinformatics, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, 030104 developmental biology, ROC Curve, RISK-FACTORS, GLUCOSE-TOLERANCE, business, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Biomarkers
Abstract: Weight loss aims to improve glycemic control in obese but strong variability is observed. Using a multi-omics approach, we investigated differences between 174 responders and 201 non-responders, that had lost >8% body weight following a low-caloric diet (LCD, 800 kcal/d for 8 weeks). The two groups were comparable at baseline for body composition, glycemic control, adipose tissue transcriptomics and plasma ketone bodies. But they differed significantly in their response to LCD, including improvements in visceral fat, overall insulin resistance (IR) and tissue-specific IR. Transcriptomics analyses found down-regulation in key lipogenic genes (e.g. SCD, ELOVL5) in responders relative to non-responders; metabolomics showed increase in ketone bodies; while proteomics revealed differences in lipoproteins. Findings were consistent between genders; with women displaying smaller improvements owing to a better baseline metabolic condition. Integrative analyses identified a plasma omics model that was able to predict non-responders with strong performance (on a testing dataset, the Receiving Operating Curve Area Under the Curve (ROC AUC) was 75% with 95% Confidence Intervals (CI) [67%, 83%]). This model was based on baseline parameters without the need for intrusive measurements and outperformed clinical models (p = 0.00075, with a +14% difference on the ROC AUCs). Our approach document differences between responders and non-responders, with strong contributions from liver and adipose tissues. Differences may be due to de novo lipogenesis, keto-metabolism and lipoprotein metabolism. These findings are useful for clinical practice to better characterize non-responders both prior and during weight loss.
Published: 2020
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9. Human milk oligosaccharides alleviate stress-induced visceral hypersensitivity and associated microbiota dysbiosis

Author: Julien Chevalier, Gabriela Bergonzelli, Guillaume Jacot, María Vicario, Laurent Favre, Léa Siegwald, Andreas Rytz, Helene Eutamene, Valérie Tondereau, Vassilia Theodorou, Laurent Ferrier, Clara Lucia Garcia-Rodenas, Andrea M. Marquard, Nestlé Institute of Health Sciences SA [Lausanne, Switzerland], Neuro-Gastroentérologie & Nutrition (ToxAlim-NGN), ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Clinical Microbiomics, and Société des Produits Nestlé.
Subjects: Male, Abdominal pain, medicine.drug_class, [SDV]Life Sciences [q-bio], Endocrinology, Diabetes and Metabolism, Clinical Biochemistry, Antibiotics, microbiome, Oligosaccharides, Distension, Gut flora, Biochemistry, antibiotics, Feces, Mice, 03 medical and health sciences, 0302 clinical medicine, medicine, Animals, Humans, Microbiome, metagenomic, Molecular Biology, 030304 developmental biology, HMOs, 0303 health sciences, Nutrition and Dietetics, Bacteria, Milk, Human, biology, business.industry, medicine.disease, biology.organism_classification, Abdominal Pain, Gastrointestinal Microbiome, Mice, Inbred C57BL, Nociception, Immunology, Dysbiosis, 030211 gastroenterology & hepatology, medicine.symptom, chronic psychological stress, business, Stress, Psychological
Abstract: International audience; Pain-related functional gastrointestinal disorders (FGIDs) are characterized by visceral hypersensitivity (VHS) associated with alterations in the microbiota-gut-brain axis. Since human milk oligosaccharides (HMOs) modulate microbiota, gut and brain, we investigated whether HMOs impact VHS and explored the role of gut microbiota. To induce VHS, C57BL/6JRj mice received hourly water avoidance stress (WAS) sessions for 10 days, or antibiotics (ATB) for 12 days. Challenged and unchallenged (Sham) animals were fed AIN93M diet (Cont) or AIN93M containing 1% of a 6-HMO mix (HMO6). VHS was assessed by monitoring the visceromotor response to colorectal distension. Fecal microbiome was analyzed by shotgun metagenomics. The effect of HMO6 sub-blends on VHS and nociceptive pathways was further tested using the WAS model. In mice fed Cont, WAS and ATB increased the visceromotor response to distension. HMO6 decreased WAS-mediated electromyographic rise at most distension volumes and overall Area Under Curve (AUC=6.12±0.50 in WAS-HMO6 vs 9.46±0.50 in WAS-Cont; p
Published: 2022
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10. Subclinical Mastitis in a European Multicenter Cohort: Prevalence, Impact on Human Milk (HM) Composition, and Association with Infant HM Intake and Growth

Author: Magnus Domellöf, Jean-Charles Picaud, Isam Al-Jashi, Michael Affolter, Kirsti Haaland, Eurídice Castañeda-Gutiérrez, Silvia Maria Stoicescu, Maria Jose Costeira, Sagar K. Thakkar, Stéphane Dubascoux, Giovanna Marchini, Tom Stiris, Carlos Antonio De Castro, Massimo Agosti, Maria Gorett Silva, Mireilla Vanpee, Cecilia Martínez-Costa, Francesca Giuffrida, Almerinda Barroso Pereira, Claude Billeaud, Irma Silva-Zolezzi, Tinu Mary Samuel, Thameur Rakza, Nestlé Institute of Health Sciences SA [Lausanne, Switzerland], CHU de Bordeaux Pellegrin [Bordeaux], Hôpital de la Croix-Rousse [CHU - HCL], Hospices Civils de Lyon (HCL), Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), AUTRES, Medicine Faculty, Titu Maiorescu University, City Bucharest, Romania., Hospital de São João [Porto], Karolinska University Hospital [Stockholm], Hôpital Jeanne de Flandres, Université de Lille, Droit et Santé-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Oslo University Hospital [Oslo], Polizu Hospital, Universitat de València (UV), and Umeå University
Subjects: Adult, 0301 basic medicine, Longitudinal study, [SDV]Life Sciences [q-bio], Mammary gland, Physiology, lcsh:TX341-641, Mastitis, Article, Cohort Studies, 03 medical and health sciences, 0302 clinical medicine, medicine, Humans, Subclinical mastitis, subclinical mastitis, Minerals, 030219 obstetrics & reproductive medicine, 030109 nutrition & dietetics, Nutrition and Dietetics, Milk, Human, business.industry, fungi, Infant, Newborn, longitudinal study, Infant, human milk, Feeding Behavior, European population, medicine.disease, Trace Elements, 3. Good health, Europe, Näringslära, Breast Feeding, medicine.anatomical_structure, Cohort, milk macronutrients, Female, business, lcsh:Nutrition. Foods and food supply, Food Science
Abstract: Background: Subclinical mastitis (SCM) is an inflammatory condition of the mammary gland. We examined the effects of SCM on human milk (HM) composition, infant growth, and HM intake in a mother&ndash, infant cohort from seven European countries. Methods: HM samples were obtained from 305 mothers at 2, 17, 30, 60, 90, and 120 days postpartum. SCM status was assessed using HM Sodium (Na): Potassium (K) ratio &gt, 0.6. Levels of different macro- and micronutrients were analyzed in HM. Results: SCM prevalence in the first month of lactation was 35.4%. Mean gestational age at delivery was lower and birth by C-section higher in SCM mothers (p &le, 0.001). HM concentrations of lactose, DHA, linolenic acid, calcium, and phosphorous (p &lt, 0.05 for all) was lower, while total protein, alpha-lactalbumin, lactoferrin, albumin, arachidonic acid to DHA ratio, n-6 to n-3 ratio and minerals (iron, selenium, manganese, zinc, and copper) were higher (p &lt, 0.001 for all) in mothers with SCM. There were no differences in infant growth and HM intake between non-SCM and SCM groups. Conclusion: We document, for the first time, in a large European standardized and longitudinal study, a high prevalence of SCM in early lactation and demonstrate that SCM is associated with significant changes in the macro- and micronutrient composition of HM. Future studies exploring the relation of SCM with breastfeeding behaviors and developmental outcomes are warranted.
Published: 2019
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11. Stem cells and the circadian clock

Author: Thomas Dickmeis, Nicolas Diotel, Benjamin D. Weger, Anne Claire Dorsemans, Meltem Weger, Univ, Réunion, University of Birmingham [Birmingham], Diabète athérothrombose et thérapies Réunion Océan Indien (DéTROI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de La Réunion (UR), Karlsruhe Institute of Technology (KIT), and Nestlé Institute of Health Sciences SA [Lausanne, Switzerland]
Subjects: Life sciences, biology, 0301 basic medicine, medicine.medical_specialty, Circadian clock, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Development, Biology, Adult neurogenesis, Models, Biological, 03 medical and health sciences, Circadian Clocks, ddc:570, Internal medicine, [SDV.BDD] Life Sciences [q-bio]/Development Biology, [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], medicine, Animals, Humans, Circadian rhythm, [SDV.BDD]Life Sciences [q-bio]/Development Biology, Molecular Biology, Stem cell, Vertebrate, Stem Cells, Neurogenesis, Cell migration, Cell Biology, Hair follicle, Embryonic stem cell, Circadian Rhythm, Cell biology, 030104 developmental biology, Endocrinology, medicine.anatomical_structure, Developmental Biology, Adult stem cell
Abstract: International audience; The circadian timing system is a complex biological network of interacting circadian clocks that regulates 24 h rhythms of behavioral and physiological processes. One intriguing observation is that stem cell homeostasis is subject to circadian clock regulation. Rhythmic oscillations have been observed in a variety of embryonic and adult stem cell dependent processes, such as hematopoietic progenitor cell migration, the hair follicle cycle, bone remodeling, regenerative myogenesis and neurogenesis. This review aims to discuss the nature of the circadian clock in embryonic stem cells and how it changes during differentiation. Furthermore, it will examine how the circadian clock contributes to adult stem cell function in different tissues of the body with an emphasis on the brain and adult neurogenesis.
Published: 2017
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12. Nutrition for the ageing brain: Towards evidence for an optimal diet

Author: Robert J. Williams, Robert Perneczky, Pascale Barberger-Gateau, Hugh Perry, Robin S.B. Williams, Maria Camprubi-Robles, Keith Wesnes, Jeroen Schuermans, Robert Clarke, Sandrine Thuret, Laura Caberlotto, Cristina Andres-Lacueva, Claudine Manach, John W.C. Sijben, Jeremy P. E. Spencer, Anne Marie Roussel, Gene L. Bowman, Sophie Miquel-Kergoat, Ondine van de Rest, María J. Ramírez, Ellen S. Mitchell, Eef Hogervorst, Amanda J. Kiliaan, Maurits Vandewoude, Ugo Lucca, Diána Bánáti, David Vauzour, Anne Marie Minihane, Norwich Medical School, University of East Anglia [Norwich] (UEA), Abbot Nutrition R&D, Wrigley, University of Barcelona, International Life Sciences Institute Europe (ILSI Europe), U 897, Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Bordeaux (UB), Nestlé Institute of Health Sciences SA [Lausanne, Switzerland], Centre for Computational and Systems Biology (COSBI), Università degli Studi di Trento (UNITN), University of Oxford, Loughborough University, Radboud University Medical Center [Nijmegen], Istituto di Ricerce Farmacologiche Mario Negri, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Imperial College London, University of Southampton, Université Joseph Fourier - Grenoble 1 (UJF), Nutricia Research, University of Reading (UOR), Maurice Wohl Clinical Neuroscience Institut, King‘s College London, King's College Hospital (KCH), Wageningen University and Research [Wageningen] (WUR), University of Antwerp (UA), Wesnes Cognition Ltd, Department of Psychology [Sheffield], University of Sheffield [Sheffield], Centre for Human Psychopharmacology, Swinburne University, Medicinal Plant Research Group, Newcastle University, University of Bath [Bath], Abbott Nutrition R&D, University of East Anglia, Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Wageningen University and Research Center (WUR), University of Antwerp, and University of Oxford [Oxford]
Subjects: 0301 basic medicine, Gerontology, MILD COGNITIVE IMPAIRMENT, Aging, Geriatrics & Gerontology, Alzheimer`s disease Donders Center for Medical Neuroscience [Radboudumc 1], Physiology, Fisiologia patològica, Cognitive decline, B400, Biochemistry, Nutritional requirements, 0302 clinical medicine, Cognition, Neuroinflammation, approche nutritionnelle, CARDIOVASCULAR RISK-FACTORS, Psychology, méthode de prévention, Cervell, Pathological physiology, senior citizens, Dietoteràpia, education.field_of_study, Preventive diet, Cognitive ageing, Envelliment cerebral, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Brain, ALZHEIMERS ASSOCIATION WORKGROUPS, Neuroprotection, 3. Good health, AMYLOID PRECURSOR PROTEIN, Neurology, SPATIAL WORKING-MEMORY, Cognició, Aging brain, Diet, Healthy, Alzheimer's disease, Nutritive Value, Life Sciences & Biomedicine, Biotechnology, Cognition disorders, RANDOMIZED CONTROLLED-TRIALS, MULTI-NUTRIENT DIET, Population, Fisiologia, Affect (psychology), Trastorns de la cognició, personne âgée, 03 medical and health sciences, POSITRON-EMISSION-TOMOGRAPHY, medicine, Humans, NITRIC-OXIDE SYNTHASE, education, Biology, Molecular Biology, maladie neurodégénérative, VLAG, Global Nutrition, Wereldvoeding, Science & Technology, Diet therapy, Nutritional Requirements, Requeriments nutricionals, 0601 Biochemistry And Cell Biology, 1103 Clinical Sciences, Cell Biology, medicine.disease, C800, Ageing, 030104 developmental biology, Clinical research, Psicologia, Nerve Degeneration, Human medicine, Cognition Disorders, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, FLAVANOL-RICH COCOA, 030217 neurology & neurosurgery
Abstract: The workshop 'Nutrition for the Ageing Brain: Towards Evidence of an Optimal Diet' was organized with funds from the ILSI Europe Nutrition and Mental Performance Task Force. Industry members of this task force are listed on the ILSI Europe website at www.ilsi.eu. For further information about ILSI Europe, please email or call +32 2 771 00 14. This review was prepared taken into account the presentations at the workshop mentioned in the abstract and was conducted by an expert group of ILSI Europe. This publication was coordinated by Mr Jeroen Schuermans, Scientific Project Manager at ILSI Europe during the proceedings process. The authors would like to acknowledge Ms Catherine Collins, Ms Lilou van Lieshout and Dr Lucie Geurts for the finalisation and submission of this manuscript; As people age they become increasingly susceptible to chronic and extremely debilitating brain diseases. The precise cause of the neuronal degeneration underlying these disorders, and indeed normal brain ageing remains however elusive. Considering the limits of existing preventive methods, there is a desire to develop effective and safe strategies. Growing preclinical and clinical research in healthy individuals or at the early stage of cognitive decline has demonstrated the beneficial impact of nutrition on cognitive functions. The present review is the most recent in a series produced by the Nutrition and Mental Performance Task Force under the auspice of the International Life Sciences Institute Europe (ILSI Europe). The latest scientific advances specific to how dietary nutrients and non-nutrient may affect cognitive ageing are presented. Furthermore, several key points related to mechanisms contributing to brain ageing, pathological conditions affecting brain function, and brain biomarkers are also discussed. Overall, findings are inconsistent and fragmented and more research is warranted to determine the underlying mechanisms and to establish dose-response relationships for optimal brain maintenance in different population subgroups. Such approaches are likely to provide the necessary evidence to develop research portfolios that will inform about new dietary recommendations on how to prevent cognitive decline
Published: 2017
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13. Perspectives on ethnic and racial disparities in Alzheimer's disease and related dementias: Update and areas of immediate need

Author: Jennifer J. Manly, Ralph N. Martins, Lon S. Schneider, Kerry Kilborn, Yakeel T. Quiroz, Scott M. Hofer, Robert A. Rissman, Octavio A. Santos, Hiroko H. Dodge, Steven D. Edland, Charlotte E. Teunissen, Ganesh M. Babulal, Hector M. González, Esther S. Oh, Donna M. Wilcock, Debora Melo van Lent, Michael Weinborn, Michelle M. Mielke, Carrie Ciro, Andre Strydom, James Hendrix, Catherine M. Roe, Roos J. Jutten, Graciela Muniz Terrera, Claudio Babiloni, Nicole Schupf, Melissa E. Murray, Benedict C. Albensi, Heather M. Snyder, Gaël Chételat, Gene L. Bowman, Elizabeth Head, Peggye Dilworth-Anderson, Anna J. Esbensen, Simone Dreux, Eider M. Arenaza-Urquijo, Adam M. Brickman, Sid E. O'Bryant, Mario A. Parra, Sietske A.M. Sikkes, Ann D. Cohen, Henrik Zetterberg, Martha Clare Morris, Leigh A. Johnson, Alex Bahar-Fuchs, Linda M.P. Wesselman, Lisbeth Evered, Keith N. Fargo, Joanne Bell, Arlene Astell, Juan Fortea, Michael Ewers, Nikolaos Scarmeas, Krista L. Lanctôt, Yaakov Stern, Hamid R. Sohrabi, Deborah Gustafson, Yi Tang, Washington University School of Medicine in St. Louis, Washington University in Saint Louis (WUSTL), Massachusetts General Hospital [Boston], Harvard Medical School [Boston] (HMS), St. Boniface Hospital Albrechtsen Research Centre [Winnipeg], University of Manitoba [Winnipeg], Mayo Clinic [Rochester], University of Toronto, University of Reading (UOR), Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome], IRCCS-Hospital San Raffaele Pisana of Rome and Cassino, University of Melbourne, Syneos Health [Wilmington], Nestlé Institute of Health Sciences SA [Lausanne, Switzerland], Oregon Health & Science University [Portland], Taub Institute for Research on Alzheimer's Disease and the Aging Brain, Columbia University [New York], Physiopathologie et imagerie des troubles neurologiques (PhIND), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), University of Kansas [Kansas City], University of Pittsburgh School of Medicine, Pennsylvania Commonwealth System of Higher Education (PCSHE), University of North Carolina [Chapel Hill] (UNC), University of North Carolina System (UNC), Harvard College [Cambridge], University of California [San Diego] (UC San Diego), University of California, University of Cincinnati (UC), Cincinnati Children's Hospital Medical Center, Klinikum der Universität [München], Medical & Scientific Relations, Alzheimer's Association [Chicago], Hospital de la Santa Creu i Sant Pau, Universitat Autònoma de Barcelona (UAB), Fundació Catalana de Síndrome de Down [Barcelona], Psychiatry and neurochemistry, SUNY Downstate Medical Center, State University of New York (SUNY), University of Kentucky, University of Victoria [Canada] (UVIC), University of North Texas Health Science Center [Fort Worth], VU University Medical Center [Amsterdam], University of Glasgow, Edith Cowan University [Joondalup], Rush University [Chicago], Mayo Clinic [Jacksonville], Johns Hopkins University School of Medicine [Baltimore], Department of Psychology [Edinburgh], Heriot-Watt University [Edinburgh] (HWU), Universidad Autonoma del Caribe [Barranquilla], University of Southern California (USC), Mailman School of Public Health Columbia University [New-York], Institute of Psychiatry, Psychology & Neuroscience, King's College London, King‘s College London, Xuan Wu Hospital [Beijing], Capital University of Medical Sciences [Beijing] (CUMS), University of Edinburgh, German Research Center for Neurodegenerative Diseases - Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE), Sahlgrenska University Hospital, University College of London [London] (UCL), University of Gothenburg (GU), Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome] (UNIROMA), University of California (UC), University of Kentucky (UK), and Duchange, Nathalie
Subjects: Gerontology, Ethnoracial, Aging, Biomedical Research, epidemiology [Alzheimer Disease], Alzheimer's related dementias, Ethnic group, Disease, Underserved, Neurodegenerative, cellular and molecular neuroscience, 0302 clinical medicine, White paper, Ethnicity, 030212 general & internal medicine, Alzheimer's Association, media_common, neurology (clinical), Diversity, Continental Population Groups, Translational, health policy, Alzheimer's disease, 3. Good health, psychiatry and mental health, epidemiology, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], geriatrics and gerontology, International Society to Advance Alzheimer's Research and Treatment, Alzheimer's Association, Population ageing, media_common.quotation_subject, Clinical Sciences, BF, Ethnic Groups, International Society to Advance Alzheimer's Research and Treatment, Article, Neglect, 03 medical and health sciences, Alzheimer Disease, Political science, medicine, diversity, ethnicity, ethnoracial, translational, underserved, developmental neuroscience, Acquired Cognitive Impairment, Dementia, Humans, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], ddc:610, Healthcare Disparities, Aged, ethnology [Alzheimer Disease], Racial Groups, Neurosciences, Alzheimer's Disease including Alzheimer's Disease Related Dementias (AD/ADRD), medicine.disease, Brain Disorders, Geriatrics, 030217 neurology & neurosurgery, Biomarkers, Diversity (politics)
Abstract: Altres ajuts: Fondo Europeo de Desarrollo Regional (FEDER), Unión Europea, "Una manera de hacer Europa", La "Marató TV3" grant (20141210 to J.F.) Alzheimer's disease and related dementias (ADRDs) are a global crisis facing the aging population and society as a whole. With the numbers of people with ADRDs predicted to rise dramatically across the world, the scientific community can no longer neglect the need for research focusing on ADRDs among underrepresented ethnoracial diverse groups. The Alzheimer's Association International Society to Advance Alzheimer's Research and Treatment (ISTAART; alz.org/ISTAART) comprises a number of professional interest areas (PIAs), each focusing on a major scientific area associated with ADRDs. We leverage the expertise of the existing international cadre of ISTAART scientists and experts to synthesize a cross-PIA white paper that provides both a concise "state-of-the-science" report of ethnoracial factors across PIA foci and updated recommendations to address immediate needs to advance ADRD science across ethnoracial populations.
Published: 2019
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14. The Mouse Microbiome Is Required for Sex-Specific Diurnal Rhythms of Gene Expression and Metabolism

Author: Aline Charpagne, Francis Foata, Jake Yeung, Benjamin D. Weger, Felix Naef, Bertrand Betrisey, Eva Martin, Frédéric Gachon, Aurélie Balvay, Sonia Jimenez, Cédric Gobet, Bernard Berger, Chieh Jason Chou, Anne Foussier, Brigitte Boizet-Bonhoure, Nestlé Institute of Health Sciences SA [Lausanne, Switzerland], Department of Diabetes and Circadian Rhythms, Nestlé Institute of Health Sciences, 1015 Lausanne, Switzerland., Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Bioengineering, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland., Department of Diabetes and Circadian Rhythms, Nestlé Institute of Health Sciences, 1015 Lausanne, Switzerland, Cellular Metabolism, Department of Cell Biology, Nestlé Institute of Health Sciences, Nestlé Research, 1015 Lausanne, Switzerland., Nestle Res Ctr, Nestec Ltd, Host-Microbe Interaction, Department of Gastro-Intestinal Health, MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Genomics, Department of Multi-Omics, Nestlé Institute of Health Sciences, Nestlé Research, 1015 Lausanne, Switzerland, Institut de génétique humaine (IGH), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Nestlé Research Center, Nestle Reasearch Center, Institute of Bioengineering, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland, Swiss National Science Foundation [310030_173079], Natural Sciences and Engineering Research Council of Canada Postgraduate Studies Doctoral Scholarship, European Project: 260988,EC:FP7:ERC,ERC-2010-StG_20091118,CIRCATRANS(2011), Nestlé Research Center | Centre de recherche Nestlé [Lausanne], Nestlé S.A., and School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland
Subjects: 0301 basic medicine, Male, Physiology, [SDV]Life Sciences [q-bio], Circadian clock, nuclear receptors, Gene Expression, White adipose tissue, Transcriptome, Mice, 0302 clinical medicine, circadian clock, reproductive function, Sex Characteristics, sexual maturation, Growth hormone secretion, symbiosis, Cell biology, Circadian Rhythm, Intestines, germ-free, ghrelin, Female, diet-induced obesity, Adipose Tissue, White, Feminization (biology), Bcl6, Biology, liver, Article, 03 medical and health sciences, Circadian Clocks, Metabolome, microbiota, Animals, Humans, Microbiome, Molecular Biology, [SDV.GEN]Life Sciences [q-bio]/Genetics, gut microbiota, aryl-hydrocarbon receptor, growth-hormone-secretion, Cell Biology, signaling pathways, Gastrointestinal Microbiome, Mice, Inbred C57BL, Sexual dimorphism, 030104 developmental biology, cyp1a1 expression, sexual dimorphism, growth hormone, 030217 neurology & neurosurgery
Abstract: Summary The circadian clock and associated feeding rhythms have a profound impact on metabolism and the gut microbiome. To what extent microbiota reciprocally affect daily rhythms of physiology in the host remains elusive. Here, we analyzed transcriptome and metabolome profiles of male and female germ-free mice. While mRNA expression of circadian clock genes revealed subtle changes in liver, intestine, and white adipose tissue, germ-free mice showed considerably altered expression of genes associated with rhythmic physiology. Strikingly, the absence of the microbiome attenuated liver sexual dimorphism and sex-specific rhythmicity. The resulting feminization of male and masculinization of female germ-free animals is likely caused by altered sexual development and growth hormone secretion, associated with differential activation of xenobiotic receptors. This defines a novel mechanism by which the microbiome regulates host metabolism., Graphical Abstract, Highlights • The microbiome is required for sexual dimorphism in gene expression and metabolism • Most already-described changes in GF mice are hallmarks of a feminized metabolism • Altered sexual maturation and GH secretion cause the damping of sexual dimorphism • Microbiota-derived metabolites and ghrelin likely drive these alterations, Physiology is dynamic over the day and different between sexes. Weger et al. show that the microbiome play a key role in sustaining these sex differences in gene expression and metabolism by ensuring proper sexual maturation and growth hormone secretion. Microbiota-derived metabolites and ghrelin likely drive these sexually dimorphic dynamics.
Published: 2019
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15. Validation of EN ISO method 15216 - Part 1 – Quantification of hepatitis A virus and norovirus in food matrices

Author: Joanna Ollivier, Gwenola Hardouin, P.H. in’t Veld, Sophie Butot, Alexandre Leclercq, Bertrand Lombard, S.A. Rutjes, James A. Lowther, Albert Bosch, D. Mäde, Centre for Environment, Fisheries and Aquaculture Science [Weymouth] (CEFAS), Universitat de Barcelona (UB), Nestlé Institute of Health Sciences SA [Lausanne, Switzerland], Laboratoire de Microbiologie, Environnement, Microbiologie et Phycotoxines (EMP), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Lower Saxony State Office for Consumer Protection and Food Safety, National Institute for Public Health and the Environment [Bilthoven] (RIVM), Association Française de Normalisation (AFNOR), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Netherlands Food and Consumer Product Safety Authority (NVWA), Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Biologie des Infections - Biology of Infection, Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), The validation of International Standard EN ISO 15216-1 was carried out under the framework of European Mandate No. M381 of DG SANTE and DG GROW (European Commission)., DIAKITE, andrée, Laboratoire de Microbiologie, IFREMER, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), and Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO)
Subjects: 0301 basic medicine, Bivalve molluscan shellfish, Standardization, [SDV]Life Sciences [q-bio], MESH: Bivalvia, medicine.disease_cause, Limit of Detection, MESH: Reverse Transcriptase Polymerase Chain Reaction, Validation, Vegetables, MESH: Animals, Risk management, MESH: Shellfish, [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Reverse Transcriptase Polymerase Chain Reaction, General Medicine, Bottled water, Hepatitis a virus, 3. Good health, Surfaces, MESH: Reproducibility of Results, [SDV] Life Sciences [q-bio], [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, MESH: Fruit, MESH: Norovirus, 030106 microbiology, MESH: Limit of Detection, Biology, Microbiology, 03 medical and health sciences, Environmental health, MESH: Hepatitis A virus, Soft fruit, medicine, MESH: European Union, Food microbiology, Animals, Humans, European Union, MESH: Food Microbiology, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, Shellfish, MESH: Humans, business.industry, International standard, Norovirus, Reproducibility of Results, Virology, MESH: Vegetables, Bivalvia, Real-time RT-PCR, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, Fruit, Food Microbiology, Hepatitis A virus, business, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Food Science
Abstract: International audience; Hepatitis A virus (HAV) and norovirus are important agents of food-borne human viral illness, with common vehicles including bivalve molluscan shellfish, soft fruit and various vegetables. Outbreaks of viral illness due to contamination of the surfaces of foods, or food preparation surfaces by for example infected food handlers are also common. Virus analysis of food matrices can contribute towards risk management for these hazards and a two-part technical specification for determination of Hepatitis A virus and norovirus in food matrices (ISO/TS 15216:2013) was published jointly by the European Committee for Standardisation and the International Organization for Standardization in 2013.As part of the European Mandate No. M381 to validate 15 standards in the field of food microbiology, an international validation study involving 18 laboratories from 11 countries in Europe was conducted between 2012 and 2014. This study aimed to generate method characteristics including limit of detection, limit of quantification, repeatability and reproducibility for ISO 15216 – Part 1, the method for quantification, in seven food matrices.The organization and results of this study, including observations that led to improvements in the standard method are presented here. After its conclusion, the method characteristics generated were added to the revised international standard, ISO 15216-1:2017, published in March 2017.
Published: 2019
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16. Structure and Function of Bovine Whey Derived Oligosaccharides Showing Synbiotic Epithelial Barrier Protective Properties

Author: Christine Cherbut, Sean Austin, Mireille Golliard, Nadine Porta, Peter I. Duncan, Juhani Saarinen, Olli Aitio, Jari Helin, Muriel Fiaux, Rafael Berrocal, Annamari Heiskanen, Norbert Sprenger, Ritva Niemelä, Denis Moënnoz, Nestlé Institute of Health Sciences SA [Lausanne, Switzerland], Glykos Finland Ltd, Nestlé Product Technology Center, Nestlé Institute of Food Safety and Analytical Sciences, and Societe des Produits Nestle S.A. Societe des Produits Nestle S.A., Switzerland
Subjects: 0301 basic medicine, Bifidobacterium longum, medicine.medical_treatment, Oligosaccharides, Synbiotics, Gut flora, medicine.disease_cause, law.invention, Enterotoxins, Probiotic, law, Intestinal Mucosa, lactobacillus probiotic, 2. Zero hunger, chemistry.chemical_classification, Nutrition and Dietetics, biology, Oligosaccharide, 3. Good health, host-microbiota interaction, Biochemistry, lcsh:Nutrition. Foods and food supply, Bacterial Toxins, 030106 microbiology, Clostridium difficile toxin A, lcsh:TX341-641, host–microbiota interaction, Protective Agents, Article, 03 medical and health sciences, Lactobacillus rhamnosus, Cell Line, Tumor, Whey, medicine, Animals, Humans, Escherichia coli, epithelial barrier function, Probiotics, Prebiotic, Galactose, biology.organism_classification, Gastrointestinal Microbiome, Lactobacillus, Prebiotics, 030104 developmental biology, chemistry, Cattle, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Food Science
Abstract: Commensal gut microbiota and probiotics have numerous effects on the host&rsquo, s metabolic and protective systems, which occur primarily through the intestinal epithelial cell interface. Prebiotics, like galacto-oligosaccharides (GOS) are widely used to modulate their function and abundance. However, important structure&ndash, function relations may exist, requiring a detailed structural characterization. Here, we detailed the structural characterization of bovine whey derived oligosaccharide preparations enriched with GOS or not, dubbed GOS-enriched milk oligosaccharides (GMOS) or MOS, respectively. We explore GMOS&rsquo, s and MOS&rsquo, s potential to improve intestinal epithelial barrier function, assessed in a model based on barrier disruptive effects of the Clostridioides difficile toxin A. GMOS and MOS contain mainly GOS species composed of &beta, 1-6- and &beta, 1-3-linked galactoses, and 3&prime, and 6&prime, sialyllactose. Both GMOS and MOS, combined with lactobacilli, like Lactobacillus rhamnosus (LPR, NCC4007), gave synergistic epithelial barrier protection, while no such effect was observed with Bifidobacterium longum (BL NCC3001), Escherichia coli (Nissle) or fructo-oligosaccharides. Mechanistically, for barrier protection with MOS, (i) viable LPR was required, (ii) acidification of growth medium was not enough, (iii) LPR did not directly neutralize toxin A, and (iv) physical proximity of LPR with the intestinal epithelial cells was necessary. This is the first study, highlighting the importance of structure&ndash, function specificity and the necessity of the simultaneous presence of prebiotic, probiotic and host cell interactions required for a biological effect.
Published: 2020
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17. Sulfoximines as potent RORγ inverse agonists

Author: Etienne Thoreau, Craig S. Harris, David Froude, Franck Bihl, Gilles Ouvry, Delphine Spiesse, Patricia Rossio, Christophe Feret, Emmanuel Vial, Laurent F. Hennequin, Jonathan Pascau, Coralie Pascau, Romain Pierre, Claire Defoin-Platel, Nathalie Taquet, Catherine Hervouet, Veronique Parnet, Olivier Christin, Guillaume Lafitte, Sophie Deret, Catherine Raffin, Claire Bouix-Peter, Anne-Pascale Luzy, Feriel Hacini-Rachinel, Branislav Musicki, Danielle Orfila, Rodolphe Vatinel, Laboratoire de synthèse organique (DCSO), École polytechnique (X)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Nestle Skin Health GALDERMA R&D, Religions et croyances (LARHRA REC ), LAboratoire de Recherche Historique Rhône-Alpes - UMR5190 (LARHRA), École normale supérieure de Lyon (ENS de Lyon)-Université Lumière - Lyon 2 (UL2)-Université Jean Moulin - Lyon 3 (UJML), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-École normale supérieure de Lyon (ENS de Lyon)-Université Lumière - Lyon 2 (UL2)-Université Jean Moulin - Lyon 3 (UJML), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Laboratoire d'Immunologie et Immunopathologie [CHU Poitiers] (CNRS URA 1172), Centre hospitalier universitaire de Poitiers (CHU Poitiers)-Centre National de la Recherche Scientifique (CNRS), Mucosal Immunity, Vaccination, and Biometrics -- Immunité des muqueuses, vaccinations et biothérapies, Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Transporteurs et Imagerie, Radiothérapie en Oncologie et Mécanismes biologiques des Altérations du Tissu Osseux (TIRO-MATOs - UMR E4320), Service Hospitalier Frédéric Joliot (SHFJ), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-UMR E4320 (TIRO-MATOs), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Côte d'Azur (UCA)-Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Côte d'Azur (UCA), Nestlé Institute of Health Sciences SA [Lausanne, Switzerland], GALDERMA R&D, Galderma Recherche et Développement [Biot, France] (Galderma R&D), Virologie humaine, École normale supérieure de Lyon (ENS de Lyon)-IFR128-Institut National de la Santé et de la Recherche Médicale (INSERM), AstraZeneca, Cancer and Infection Research, Université Nice Sophia Antipolis (... - 2019) (UNS), École normale supérieure - Lyon (ENS Lyon)-Université Lumière - Lyon 2 (UL2)-Université Jean Moulin - Lyon 3 (UJML), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-École normale supérieure - Lyon (ENS Lyon)-Université Lumière - Lyon 2 (UL2)-Université Jean Moulin - Lyon 3 (UJML), Centre International de Recherche en Infectiologie - UMR (CIRI), Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), UMR E4320 (TIRO-MATOs), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Côte d'Azur (UCA)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Côte d'Azur (UCA)-Service Hospitalier Frédéric Joliot (SHFJ), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, and École normale supérieure - Lyon (ENS Lyon)-IFR128-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Drug Inverse Agonism, [SDV]Life Sciences [q-bio], Clinical Biochemistry, Pharmaceutical Science, Context (language use), Pharmacology, Ligands, 010402 general chemistry, 01 natural sciences, Biochemistry, Structure-Activity Relationship, chemistry.chemical_compound, In vivo, Drug Discovery, Animals, Humans, Inverse agonist, Potency, [CHIM]Chemical Sciences, Hydroxymethyl, Molecular Biology, ComputingMilieux_MISCELLANEOUS, Mice, Inbred BALB C, Sulfonamides, Molecular Structure, 010405 organic chemistry, Organic Chemistry, Stereoisomerism, Biological activity, Nuclear Receptor Subfamily 1, Group F, Member 3, 0104 chemical sciences, 3. Good health, chemistry, Nuclear receptor, Sulfoxides, Lipophilicity, Molecular Medicine, Female, Imines
Abstract: Progress in the identification of suitable RORγ inverse agonists as clinical candidates has been hampered by the high lipophilicity that seems required for high potency on this nuclear receptor. In this context, we decided to focus on the replacement of the hydroxymethyl group found on known modulators to determine if more polarity could be tolerated in this position. SAR of the replacement of this moiety is presented in this article leading to the identification of sulfoximine derivatives as potent modulators with pharmacological activity in the in vivo mouse Imiquimod psoriasis model.
Published: 2018
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18. Discovery and Characterization of CD12681, a Potent RORγ Inverse Agonist, Preclinical Candidate for the Topical Treatment of Psoriasis

Author: Coralie Pascau, Nicolas Atrux-Tallau, Laurent F. Hennequin, Christophe Feret, Gilles Ouvry, Feriel Hacini-Rachinel, Craig S. Harris, Claire Defoin-Platel, Nathalie Taquet, Sophie Deret, Mathieu Forissier, Hélène Huguet, Etienne Thoreau, Benjamin Ozello, Olivier Christin, Emmanuel Vial, Guillaume Lafitte, Danielle Orfila, Isabelle Carlavan, Franck Bihl, David Froude, Jean-François Fournier, Denis Duvert, Anne-Pascale Luzy, Delphine Spiesse, Patricia Rossio, Marie-Josée Cuadrado, Jonathan Pascau, Catherine Raffin, Aline Bondu, Claire Bouix-Peter, Catherine Hervouet, Guillaume Peluchon, Veronique Parnet, David Piwnica, Branislav Musicki, Rodolphe Vatinel, Romain Pierre, Laboratoire de synthèse organique (DCSO), École polytechnique (X)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Nestle Skin Health GALDERMA R&D, Religions et croyances (LARHRA REC ), LAboratoire de Recherche Historique Rhône-Alpes - UMR5190 (LARHRA), École normale supérieure de Lyon (ENS de Lyon)-Université Lumière - Lyon 2 (UL2)-Université Jean Moulin - Lyon 3 (UJML), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-École normale supérieure de Lyon (ENS de Lyon)-Université Lumière - Lyon 2 (UL2)-Université Jean Moulin - Lyon 3 (UJML), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Laboratoire d'Immunologie et Immunopathologie [CHU Poitiers] (CNRS URA 1172), Centre hospitalier universitaire de Poitiers (CHU Poitiers)-Centre National de la Recherche Scientifique (CNRS), Mucosal Immunity, Vaccination, and Biometrics -- Immunité des muqueuses, vaccinations et biothérapies, Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Transporteurs et Imagerie, Radiothérapie en Oncologie et Mécanismes biologiques des Altérations du Tissu Osseux (TIRO-MATOs - UMR E4320), Service Hospitalier Frédéric Joliot (SHFJ), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-UMR E4320 (TIRO-MATOs), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Côte d'Azur (UCA)-Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Côte d'Azur (UCA), Nestlé Institute of Health Sciences SA [Lausanne, Switzerland], Institut de Recherches SERVIER (IRS), GALDERMA R&D, Galderma Recherche et Développement [Biot, France] (Galderma R&D), Virologie humaine, École normale supérieure de Lyon (ENS de Lyon)-IFR128-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Nice Sophia Antipolis (... - 2019) (UNS), École normale supérieure - Lyon (ENS Lyon)-Université Lumière - Lyon 2 (UL2)-Université Jean Moulin - Lyon 3 (UJML), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-École normale supérieure - Lyon (ENS Lyon)-Université Lumière - Lyon 2 (UL2)-Université Jean Moulin - Lyon 3 (UJML), Centre International de Recherche en Infectiologie - UMR (CIRI), Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), UMR E4320 (TIRO-MATOs), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Côte d'Azur (UCA)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Côte d'Azur (UCA)-Service Hospitalier Frédéric Joliot (SHFJ), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, and École normale supérieure - Lyon (ENS Lyon)-IFR128-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: 0301 basic medicine, Keratinocytes, Administration, Topical, [SDV]Life Sciences [q-bio], Retinoic acid, Drug Evaluation, Preclinical, Pharmacology, 01 natural sciences, Biochemistry, Interleukin-23, chemistry.chemical_compound, Mice, Drug Discovery, heterocyclic compounds, General Pharmacology, Toxicology and Pharmaceutics, ComputingMilieux_MISCELLANEOUS, Orphan receptor, Mice, Inbred BALB C, Sulfonamides, Interleukin-17, Nuclear Receptor Subfamily 1, Group F, Member 3, 3. Good health, Molecular Medicine, medicine.symptom, Drug Inverse Agonism, Inflammation, Topical treatment, Skin Diseases, 03 medical and health sciences, Inhibitory Concentration 50, Structure-Activity Relationship, In vivo, Psoriasis, medicine, Inverse agonist, Animals, Humans, [CHIM]Chemical Sciences, 010405 organic chemistry, business.industry, Organic Chemistry, medicine.disease, 0104 chemical sciences, Disease Models, Animal, 030104 developmental biology, chemistry, Mouse skin, Th17 Cells, business
Abstract: With possible implications in multiple autoimmune diseases, the retinoic acid receptor-related orphan receptor RORγ has become a sought-after target in the pharmaceutical industry. Herein are described the efforts to identify a potent RORγ inverse agonist compatible with topical application for the treatment of skin diseases. These efforts culminated in the discovery of N-(2,4-dimethylphenyl)-N-isobutyl-2-oxo-1-[(tetrahydro-2H-pyran-4-yl)methyl]-2,3-dihydro-1H-benzo[d]imidazole-5-sulfonamide (CD12681), a potent inverse agonist with in vivo activity in an IL-23-induced mouse skin inflammation model.
Published: 2018
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19. Lipidomics reveals diurnal lipid oscillations in human skeletal muscle persisting in cellular myotubes cultured in vitro

Author: Ursula Loizides-Mangold, Leonidas G. Karagounis, Bart Vandereycken, Howard Riezman, Benjamin D. Weger, Laurent Perrin, Etienne Lefai, Marc Moniatte, Stéphanie Chanon, Frédéric Gachon, Iain Templeman, Charna Dibner, Jean-Philippe Walhin, Maud Robert, Jonathan Paz Montoya, Christine Durand, Jonathan D. Johnston, James A. Betts, Geneva University Hospital (HUG), Section de mathématiques [Genève], Université de Genève (UNIGE), University of Bath [Bath], Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), Nestlé Institute of Health Sciences SA [Lausanne, Switzerland], Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon (HCL), Ecole Polytechnique Fédérale de Lausanne (EPFL), Plymouth University, University of Surrey (UNIS), Sinergia Swiss National Science Foundation [CRSII3-154405], Swiss National Science Foundation [31003A-166700], [310030B-166686], National Centre of Competence in Research Chemical Biology, Fondation Romande pour la Recherche sur le Diabete, Fondation Ernst et Lucie Schmidheiny, Societe Academique de Geneve, United Kingdom Biotechnology and Biological Sciences Research Council [BB/I008470/1], Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), and Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL)
Subjects: 0301 basic medicine, Muscle tissue, [SDV]Life Sciences [q-bio], Circadian clock, Muscle Fibers, Skeletal, Biology, In Vitro Techniques, Cell Physiological Phenomena, 03 medical and health sciences, Lipidomics, circadian clock, lipid metabolism, medicine, Homeostasis, Humans, human primary myotubes, ddc:510, Muscle, Skeletal, ddc:612, Cells, Cultured, ComputingMilieux_MISCELLANEOUS, ddc:616, Multidisciplinary, Myogenesis, Skeletal muscle, Lipid metabolism, human skeletal muscle, Sphingolipid, Lipids, Healthy Volunteers, Circadian Rhythm, CLOCK, 030104 developmental biology, medicine.anatomical_structure, Biochemistry, PNAS Plus, ddc:540, lipidomics, lipids (amino acids, peptides, and proteins)
Abstract: International audience; Circadian clocks play an important role in lipid homeostasis, with impact on various metabolic diseases. Due to the central role of skeletal muscle in whole-body metabolism, we aimed at studying muscle lipid profiles in a temporal manner. Moreover, it has not been shown whether lipid oscillations in peripheral tissues are driven by diurnal cycles of rest-activity and food intake or are able to persist in vitro in a cell-autonomous manner. To address this, we investigated lipid profiles over 24 h in human skeletal muscle in vivo and in primary human myotubes cultured in vitro. Glycerolipids, glycerophos-pholipids, and sphingolipids exhibited diurnal oscillations, suggesting a widespread circadian impact on muscle lipid metabolism. Notably, peak levels of lipid accumulation were in phase coherence with core clock gene expression in vivo and in vitro. The percentage of oscillating lipid metabolites was comparable between muscle tissue and cultured myotubes, and temporal lipid profiles correlatedwith transcript profiles of genes implicated in their biosynthesis. Lipids enriched in the outer leaflet of the plasma membrane oscillated in a highly coordinated manner in vivo and in vitro. Lipid metabolite oscillations were strongly attenuated upon siRNA-mediated clock disruption in human primary myotubes. Taken together, our data suggest an essential role for endogenous cell-autonomous human skeletal muscle oscillators in regulating lipid metabolism independent of external synchronizers, such as physical activity or food intake.
Published: 2017
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20. The cAMP response element binding protein-2 (CREB-2) can interact with the C/EBP-homologous protein (CHOP)

Author: Jean-Michel Mesnard, Joseph Aman Koffi, Sabine Thébault, Jihane Basbous, Frédéric Gachon, Christian Devaux, Gilles Gaudray, Nestlé Institute of Health Sciences SA [Lausanne, Switzerland], Infections rétrovirales et signalisation cellulaire (IRSC), Université Montpellier 1 (UM1)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche en Infectiologie de Montpellier (IRIM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut de biologie moléculaire des plantes (IBMP), and Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)
Subjects: Transcription, Genetic, [SDV]Life Sciences [q-bio], Biochemistry, 0302 clinical medicine, Structural Biology, hemic and lymphatic diseases, Chlorocebus aethiops, cAMP response element binding protein-2, Promoter Regions, Genetic, ComputingMilieux_MISCELLANEOUS, Human T-cell leukemia virus type I, Transcription Factor CHOP, Human T-lymphotropic virus 1, 0303 health sciences, Ccaat-enhancer-binding proteins, biology, Chemistry, 3. Good health, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, 030220 oncology & carcinogenesis, Basic leucine zipper factor, COS Cells, Gene Products, tat, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, tat Gene Products, Human Immunodeficiency Virus, CREB1, Gene Expression Regulation, Viral, Leucine zipper, Recombinant Fusion Proteins, Biophysics, Saccharomyces cerevisiae, CREB, cAMP response element site, Cell Line, 03 medical and health sciences, Two-Hybrid System Techniques, Genetics, Animals, Humans, C/EBP-homologous protein, Molecular Biology, CAMP response element binding, 030304 developmental biology, Cell Nucleus, Leucine Zippers, ATF3, Activator (genetics), Cell Biology, Activating Transcription Factor 4, Molecular biology, CCAAT-Enhancer-Binding Proteins, HIV-1, biology.protein, Transcription Factors
Abstract: cAMP response element binding protein-2 (CREB-2) is a basic leucine zipper (bZIP) factor that was originally described as a repressor of CRE-dependent transcription but that can also act as a transcriptional activator. Moreover, CREB-2 is able to function in association with the viral Tax protein as an activator of the human T-cell leukemia virus type I (HTLV-I) promoter. Here we show that CREB-2 is able to interact with C/EBP-homologous protein (CHOP), a bZIP transcription factor known to inhibit CAAT/enhancer-dependent transcription. Cotransfection of CHOP with CREB-2 results in decreased activation driven by the cellular CRE motif or the HTLV-I proximal Tax-responsive element, confirming that CREB-2 and CHOP can interact with each other in vivo.
Published: 2001
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20 results on '"Nestlé Institute of Health Sciences SA [Lausanne, Switzerland]"'

1. Effect of an enteral amino acid blend on muscle and gut functionality in critically ill patients: a proof-of-concept randomized controlled trial

2. OBEDIS Core Variables Project: European Expert Guidelines on a Minimal Core Set of Variables to Include in Randomized, Controlled Clinical Trials of Obesity Interventions

3. The Dietary Inflammatory Index Is Associated with Subclinical Mastitis in Lactating European Women

4. High hydrostatic pressure processing of human milk preserves milk oligosaccharides and avoids formation of Maillard reaction products

5. Network Analyses Reveal Negative Link Between Changes in Adipose Tissue GDF15 and BMI During Dietary-induced Weight Loss

6. Dietary sialylated oligosaccharides in early-life may promote cognitive flexibility during development in context of obesogenic dietary intake

7. Analysis of dietary patterns and nutritional adequacy in lactating women : a multicentre European cohort (ATLAS study)

8. Integrative phenotyping of glycemic responders upon clinical weight loss using multi-omics

9. Human milk oligosaccharides alleviate stress-induced visceral hypersensitivity and associated microbiota dysbiosis

10. Subclinical Mastitis in a European Multicenter Cohort: Prevalence, Impact on Human Milk (HM) Composition, and Association with Infant HM Intake and Growth

11. Stem cells and the circadian clock

12. Nutrition for the ageing brain: Towards evidence for an optimal diet

13. Perspectives on ethnic and racial disparities in Alzheimer's disease and related dementias: Update and areas of immediate need

14. The Mouse Microbiome Is Required for Sex-Specific Diurnal Rhythms of Gene Expression and Metabolism

15. Validation of EN ISO method 15216 - Part 1 – Quantification of hepatitis A virus and norovirus in food matrices

16. Structure and Function of Bovine Whey Derived Oligosaccharides Showing Synbiotic Epithelial Barrier Protective Properties

17. Sulfoximines as potent RORγ inverse agonists

18. Discovery and Characterization of CD12681, a Potent RORγ Inverse Agonist, Preclinical Candidate for the Topical Treatment of Psoriasis

19. Lipidomics reveals diurnal lipid oscillations in human skeletal muscle persisting in cellular myotubes cultured in vitro

20. The cAMP response element binding protein-2 (CREB-2) can interact with the C/EBP-homologous protein (CHOP)

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