1. Remote imaging of single cell 3D morphology with ultrafast coherent phonons and their resonance harmonics
- Author
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Liu, Liwang, Viel, Alexis, Saux, Guillaume, Plawinski, Laurent, Muggiolu, Giovanna, BARBERET, Philippe, Pereira, Marco, Ayela, Cédric, Seznec, Hervé, Durrieu, Marie-Christine, Olive, Jean-Marc, Audoin, Bertrand, Institut de Mécanique et d'Ingénierie de Bordeaux (I2M), École Nationale Supérieure d'Arts et Métiers (ENSAM), Arts et Métiers Sciences et Technologies, HESAM Université (HESAM)-HESAM Université (HESAM)-Arts et Métiers Sciences et Technologies, HESAM Université (HESAM)-HESAM Université (HESAM)-Institut Polytechnique de Bordeaux-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB), Chimie et Biologie des Membranes et des Nanoobjets (CBMN), Université Sciences et Technologies - Bordeaux 1-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Centre National de la Recherche Scientifique (CNRS), Centre d'Etudes Nucléaires de Bordeaux Gradignan (CENBG), Université Sciences et Technologies - Bordeaux 1-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de l'intégration, du matériau au système (IMS), Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS), Univ Bordeaux, CNRS UMR 5248, Inst Chim & Biol Membranes & Nanoobjets, INP Bordeaux, Université de Bordeaux (UB)-Institut Polytechnique de Bordeaux (Bordeaux INP)-Centre National de la Recherche Scientifique (CNRS), Univ. Bordeaux, CNRS, Bordeaux INP, IMS, UMR 5218, F-33400, Talence, France (IMS), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-École Nationale Supérieure d'Arts et Métiers (ENSAM), HESAM Université (HESAM)-HESAM Université (HESAM)-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS), and École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
[SPI.OTHER]Engineering Sciences [physics]/Other ,Optics and Photonics ,Acoustics, Imaging, Imaging techniques, Photoacoustics ,lcsh:Medicine ,Imaging techniques ,Monocytes ,Imaging ,Imaging, Three-Dimensional ,Cell Line, Tumor ,TOMOGRAPHY ,Humans ,Polymethyl Methacrylate ,LIVING CELLS ,lcsh:Science ,Cell Shape ,DIFFERENTIAL CONFOCAL MICROSCOPY ,ATOMIC-FORCE MICROSCOPY ,[PHYS]Physics [physics] ,Osteosarcoma ,Science & Technology ,Photoacoustics ,Macrophages ,lcsh:R ,BREAST-CANCER CELLS ,[CHIM.MATE]Chemical Sciences/Material chemistry ,Acoustics ,Multidisciplinary Sciences ,RESOLUTION ,ACOUSTIC MICROSCOPY ,MORPHOGENESIS ,Phonons ,Science & Technology - Other Topics ,SHAPE ,lcsh:Q ,Single-Cell Analysis ,REFRACTIVE-INDEX - Abstract
Cell morphological analysis has long been used in cell biology and physiology for abnormality identification, early cancer detection, and dynamic change analysis under specific environmental stresses. This work reports on the remote mapping of cell 3D morphology with an in-plane resolution limited by optics and an out-of-plane accuracy down to a tenth of the optical wavelength. For this, GHz coherent acoustic phonons and their resonance harmonics were tracked by means of an ultrafast opto-acoustic technique. After illustrating the measurement accuracy with cell-mimetic polymer films we map the 3D morphology of an entire osteosarcoma cell. The resulting image complies with the image obtained by standard atomic force microscopy, and both reveal very close roughness mean values. In addition, while scanning macrophages and monocytes, we demonstrate an enhanced contrast of thickness mapping by taking advantage of the detection of high-frequency resonance harmonics. Illustrations are given with the remote quantitative imaging of the nucleus thickness gradient of migrating monocyte cells. ispartof: SCIENTIFIC REPORTS vol:9 issue:1 ispartof: location:England status: published
- Published
- 2019