1. Keratinocyte Motility Is Affected by UVA Radiation—A Comparison between Normal and Dysplastic Cells
- Author
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Andreea O. Urs, Cristina M. Niculiţe, Mircea Leabu, Laura Cristina Ceafalan, Marina Tamara Nechifor, and Laura Olariu
- Subjects
Keratinocytes ,0301 basic medicine ,actin cytoskeleton ,Cell Survival ,Ultraviolet Rays ,dysplastic cell ,Cell ,Motility ,keratinocyte ,wound healing ,cell motility ,Article ,Catalysis ,Inorganic Chemistry ,lcsh:Chemistry ,03 medical and health sciences ,Cell Movement ,Cell Adhesion ,medicine ,Humans ,Physical and Theoretical Chemistry ,skin and connective tissue diseases ,Molecular Biology ,lcsh:QH301-705.5 ,Spectroscopy ,integumentary system ,Chemistry ,Organic Chemistry ,focal contacts ,General Medicine ,Actin cytoskeleton ,Actins ,Computer Science Applications ,Cell biology ,HaCaT ,030104 developmental biology ,medicine.anatomical_structure ,lcsh:Biology (General) ,lcsh:QD1-999 ,Cell culture ,sense organs ,Keratinocyte ,Wound healing ,Filopodia - Abstract
UVA radiation induces multiple and complex changes in the skin, affecting epidermal cell behavior. This study reports the effects of UVA exposure on normal (HaCaT) and dysplastic (DOK) keratinocytes. The adherence, spreading and proliferation were investigated by time-lapse measurement of cell layer impedance on different matrix proteins. Prior to UVA exposure, the time required for adherence and spreading did not differ significantly for HaCaT and DOK cells, while spreading areas were larger for HaCaT cells. Under UVA exposure, HaCaT and DOK cells behavior differed in terms of movement and proliferation. The cells&rsquo, ability to cover the denuded surface and individual cell trajectories were recorded by time-lapse videomicroscopy, during wound healing experiments. Dysplastic keratinocytes showed more sensitivity to UVA, exhibiting transient deficiencies in directionality of movement and a delay in re-coating the denuded area. The actin cytoskeleton displayed a cortical organization immediately after irradiation, in both cell lines, similar to mock-irradiated cells. Post-irradiation, DOK cells displayed a better organization of stress fibers, persistent filopodia, and new, stronger focal contacts. In conclusion, after UVA exposure HaCaT and DOK cells showed a different behavior in terms of adherence, spreading, motility, proliferation, and actin cytoskeleton dynamics, with the dyplastic keratinocytes being more sensitive.
- Published
- 2018