1. Celastrol-conjugated chitosan oligosaccharide for the treatment of pancreatic cancer
- Author
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Xiaohu Zeng, Xin Zhu, Qikang Tian, Xiaoke Tan, Ning Sun, Min Yan, Junwei Zhao, Xiangxiang Wu, Ruiqin Li, Zhenqiang Zhang, and Huahui Zeng
- Subjects
Cell Survival ,Chemistry, Pharmaceutical ,pancreatic cancer ,Pharmaceutical Science ,Oligosaccharides ,Antineoplastic Agents ,Apoptosis ,RM1-950 ,Mice ,Cell Line, Tumor ,Animals ,Humans ,drug delivery system ,Particle Size ,celastrol ,Chitosan ,Mice, Inbred BALB C ,toxicity ,General Medicine ,Tumor Burden ,Pancreatic Neoplasms ,Drug Liberation ,Solubility ,chitosan oligosaccharide ,Therapeutics. Pharmacology ,Pentacyclic Triterpenes ,Research Article - Abstract
Celastrol is a promising antitumor drug candidate, but the poor water solubility and cytotoxicity limit its clinical application. Herein, we synthesized a Celastrol (Cel)-chitosan oligosaccharide (CSO) conjugate (Cel-CSO) for drug delivery. Celastrol was conjugated to a CSO backbone via amide bond formation, which was verified by infrared spectrum (IR) analyses. The Cel-CSO contained ∼10 wt% of Celastrol showed excellent aqueous solubility (18.6 mg/mL) in comparation with the parent Celastrol. Cel-CSO significantly inhibited tumor growth, induced apoptosis, and effectively suppressed tumor metastasis in human pancreatic cancer cells (BxPC-3). While the cytotoxicity of Cel-CSO in hepatic cells (HL7702) was lower than that of the free Celastrol. Cel-CSO enhanced the anticancer efficacy, promoted the circulation time of Celastrol, and reduced the subacute toxicity, which indicated that CSO can be a promising Celastrol delivery system for pancreatic cancer therapy.
- Published
- 2022