1. The development of specific rRNA-derived oligonucleotide probes for Haemophilus ducreyi, the causative agent of chancroid
- Author
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Maddy Duhamel, Hugo Van Heuverswyn, Jean Luc Decourt, Rudi Rossau, and Geert Jannes
- Subjects
Molecular Sequence Data ,Biology ,Polymerase Chain Reaction ,Microbiology ,Chancroid ,Haemophilus ducreyi ,Cistron ,23S ribosomal RNA ,RNA, Ribosomal, 16S ,Humans ,Cloning, Molecular ,Genetics ,Base Sequence ,Oligonucleotide ,Nucleic acid sequence ,Nucleic Acid Hybridization ,Ribosomal RNA ,biology.organism_classification ,16S ribosomal RNA ,Molecular biology ,RNA, Bacterial ,RNA, Ribosomal, 23S ,Nucleic Acid Conformation ,Oligonucleotide Probes ,Molecular probe ,Sequence Alignment - Abstract
Summary Part of a ribosomal ribonucleic acid (rRNA) cistron of Haemophilus ducreyi was enzymically amplified using conserved primers within the rRNA molecules, cloned in a plasmid vector, and sequenced. From the nucleotide sequence, eight oligonucleotides complementary to different regions in the 16S and 23S rRNA molecules were selected, chemically synthesized, and used as hybridization probes. Hybridization experiments with at least 41 H. ducreyi strains and 13 or 14 non-H. ducreyi strains revealed that all eight oligonucleotide probes were highly reliable and completely specific for H. ducreyi strains. Comparisons of 16S rRNA sequences confirm that H. ducreyi is a member of the Pasteurellaceae though not closely related to other species in this family.
- Published
- 1991
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