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The research described here was focused on the effect on human intestinal epithelial cell monolayers of sulfated red algal polysaccharides (κ-, λ-, and κ/β-carrageenans) alone and in combination with casein or lipopolysaccharide (LPS). HT-29 cells were investigated under normal and stress conditions; stress was induced by exposure to ethanol. Cell viability was monitored with a real-time system. The change in binding properties of negatively sulfated red algal polysaccharides assessed by the measurement of free carrageenans in mixtures with casein or McCoy's 5 A culture medium by means of toluidine blue O. Low sulfate content and the presence of 3,6-anhydogalactose are prerequisites for the recovery of ethanol-exposed HT-29 cells by carrageenans. Analysis of carrageenan binding ability confirmed that casein and LPS should affect carrageenan activity. Whether the combined action of the mucin-containing layer and carrageenans or the action of carrageenans alone was responsible for enhanced cell viability under stress conditions induced by ethanol is a subject for further research. © 2017 Wiley Periodicals Inc. J Biomed Mater Res Part A: 105A: 2843-2850, 2017.

Development of targeted drug delivery system is key problem of cancer gene therapy. To ensure specific delivery of these therapeutic compounds to the tumor it is preferable for therapeutic gene expression to occur predominantly in cancer cells. Therefore, when testing drug in vivo, it is necessary to study distribution of therapeutic gene expression products in different tissues of the organism. Sodium iodide symporter (NIS) is attractive reporter because its tissue level is easily quantitatively detected by noninvasive imaging methods. Different promoters are used to direct expression of therapeutic genes in tumor cells: strong nonspecific, moderate tissue-specific and tumor-specific. Tumor-specific promoters function in wide range of tumor cells, however they are relatively weak. Relationship between promoter and sodium iodide symporter activity is unclear to date. In this report we examined activity of different promoters in two melanoma cell lines, functional activity of NIS driven by these promoters, also we compared promoter strength and NIS activity. We demonstrated that in spite of strong differences in promoter activity functional activity of NIS directed by these promoters varies weakly. Relatively weak melanoma-specific promoter directs high NIS activity in melanoma cell, however weaker cancer-specific promoters drive high NIS activity only in certain melanoma cell line.

We have analyzed morphologic and chronologic characteristics of epileptiform activity, with account for repeated EEG-study during the follow-up, in patients with periventricular leukomalacia and children cerebral palsy without epilepsy. The high frequency of "benign epileptiform patterns of childhood" (BEPC) was noted. The epileptiform activity recorded by chronologic criteria corresponded to BEPC in 67% of children. The high probability of epileptiform activity of symptomatic character was identified in 33% of children. The results obtained in this study of the parameters of epileptiform activity could be of great importance for predicting the risk for the development of epilepsy and tactics of rehabilitation of motor disorders.

Experience of treatment of 103 patients with an acute thromboembolism (TE) of a. pulmonalis (AP) was analyzed. According to clinic, angiographical and hemodynamical investigations data the indications for performance of operative and conservative treatment depending on degree of the pulmonary blood flow disorder were substantiated. Comparative analysis of results of treatment of TEAP using different thromboembolic preparations was conducted. Efficacy of thrombolysis depends on time passed away from moment of lesion. In 69% patients thromboembolectomy is effective.

The authors observed 32 cases of pneumothorax which developed as a result of doctors' manipulation: after pleural punction (10), hemosorption (5), acupuncture (4), puncture of vena subclavia (8), tracheostomy (2), manual therapy (2), installation of provisional artificial pacemaker (1). Diagnosis of complication was confirmed roentgenologically, nature of lung lesion was specified by thoracoscopy. Pleural punction (in 4 cases) and pleural drainage (in 28) were undertaken for the management of pneumothorax.

The papers considers the main methods of mathematical description of light-biological tissue interactions in the context of problems of transcutaneous reflective oximetry. Provides a review of the most significant studies which have been recently performed in this field. By using them as an example, the paper analyzes the most serious problems arising in designing reflective oximeters and the main ways to solve them. The paper also discusses the advantages of the reflective oximeter and prospects for their development.

A detailed clinico-laboratory examination of workers of Ternopol sewing factories revealed in 13.9% chronic inflammatory diseases of the hepatobiliary system which exceeds 4 times the indices of seeking medical advice (3.5%) mainly due to latent course. Entropy of blood serum protein data are more informative than bilirubin and transaminase data used in evolution of homeostasis disorders in these patients.

A method for assays of human peripheral blood natural killers, making use of monoclonal antibodies, has been developed. The method is unsophisticated, reagent-saving, does not involve the use of special equipment. Comparison of the results of the cytotoxic method 'with those of routine tests has shown a good correlation of the results.

A study is presented of 94 patients with chronic inflammatory diseases of the hepatobiliary system and in 22 patients with dyskinesia of the biliary tract. The importance of the complement consumption reaction was evaluated (N. I. Kondrashova modification). The results of the investigation revealed distinct increase of the level of antitissue antibodies in patients with chronic inflammatory diseases of the hepatobiliary system at the stage of exacerbation. This may be used for assessment of the activity of the pathological process, differential diagnosis of biliary tract dyskinesia and as an index of treatment efficacy.

A study of system of cellular and humoral immunity, unspecific defense in 187 patients with diffuse toxic goiter before and after treatment with mercasolyl revealed that despite the clinical effect, the agent did not essentially influence the content and functional activity of T-lymphocytes. The effect was more favourable on the content of immunoglobulins, titer of antithyroid antibodies. This was observed in mild and average severe course of the disease. It is suggested that mercasolyl effects lymphocytes synthesizing autoantibodies to the thyroid gland.