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In the previous paper, the present authors reported on diminution in agglutinability of trypsin-treated M and MN red cells with anti-M IgG antibody. This paper deals with the time course of changes in agglutinability and quantitative differences of M agglutinogen of red cells. M agglutinogen of M red cells disappeared much faster than that of MN, and the lowest titer of anti-M IgG antibody to agglutinate M red cells was higher than that to agglutinate MN red cells. The significant difference of agglutination scores was observed in the frequency distribution in 100 cases of M and MN red cells each. Subgroups such as M1 and M2 in M agglutinogen of trypsin-treated red cells reacting with anti-M IgG antibody were unsettled.

An 8-year-old girl with myelocytic leukemia was found to have blood group B by detection of B agglutinogen and anti-A agglutinin, agglutinin absorption test, elution test and family studies, but her red cells not agglutinated by anti-B serum amounted to 83% in the peripheral and bone marrow blood, and the red cells failed to react with anti-H. By the microprecipitation method, HbF-cells were found to occupy 79.8% of the whole red cells and 87.9% of the non-agglutinated red cells at the first examination. Thirty-seven days later, fetal hemoglobin containing red cells were 78.1% of the whole red cells, 87.1% of the non-agglutinated red cells, 58.2% of the agglutinated ones and 88.8% of the non-agglutinated red cells treated with bromelin. The quantity of HbF tested by the alkali denaturation method amounted to 54.8% of the non-agglutinated and 28.8% of the agglutinated red cells. HbF components were increased and carbonic anhydrase was reduced to a low level in the patient's blood.

KUMAGAI, R., TOKIWA, K., NAKAYASHIKI, N. and KATSURA, S. Glutamate- Pyruvate Transaminase (GPT) Variant Found in a Disputed Paternity Case. Tohoku J. exp. Med., 1987, 153 (4), 383-388 - GPT variants, GPT2C-1 and GPT2A-2C, were found in samples from a mother and her child, respectively, in a disputed paternity case. The phenotype GPT 2C-1 was determined by polyacrylamide gel electrophoresis performed at alkaline pH and acidic pH; this is probably the first GPT2C-1 detected in Japanese. It was considered that the GPT*2C allele in this family was inherited in a manner of autosomal codominant transmission.

The presence of M and N antigens in the blood components other than the red cells and various tissues has not been settled yet. The elution method in combination with antiglobulin test reported by the present authors has been used for grouping the stains of the leucocytes, platelets, fibrin, and thymic lymphocytes. All of the test-stains showed obviously positive antigen activities which coincided with blood group of the sources. The experiments of the test-stains treated with trypsin revealed that the treated stains showed a similar agglutin-ability to that of the treated red cells. From the results, it is concluded that the blood components have M or N antigen activity which is a little lower than the red cells.

This paper deals with MN blood grouping of stains of organ homogenate and thin organ sections by means of an elution test in combination with antiglobulin test. Each stain of the liver, spleen, brain, heart, lung, thyroid, kidney and muscle homogenates gave evidently M and N blood group activities in accordance with those of sources. It was considered that non-specific adsorbing potency of the human organ was eliminated in the procedure of homogenate-staining. However, examination on sections showed frequently unsatisfactory results. It was attributed to non-specific adsorption of sections with anti-M and -N antibodies. The present findings may offer a valuable information to identify human tissues in forensic practice.