1. Development of an immunochromatographic point-of-care test for detection of IgG antibody in serodiagnosis of human trichinellosis
- Author
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Somjintana Tourtip, Pewpan M. Intapan, Penchom Janwan, Rutchanee Rodpai, Patcharaporn Boonroumkaew, Wanchai Maleewong, Lakkhana Sadaow, Oranuch Sanpool, and Tongjit Thanchomnang
- Subjects
Microbiology (medical) ,medicine.medical_specialty ,Human Trichinellosis ,Trichinella ,Point-of-care testing ,Trichinella spiralis ,Antibodies, Helminth ,Screening test ,Enzyme-Linked Immunosorbent Assay ,Infectious and parasitic diseases ,RC109-216 ,Antigen ,Positive predicative value ,Internal medicine ,Diagnosis ,parasitic diseases ,medicine ,Humans ,Serologic Tests ,Retrospective Studies ,Rapid test ,biology ,business.industry ,Trichinellosis ,General Medicine ,medicine.disease ,biology.organism_classification ,Infectious Diseases ,Point-of-Care Testing ,Antigens, Helminth ,Immunoglobulin G ,Parasitic disease ,biology.protein ,Immunochromatographic test ,Antibody ,business - Abstract
Objective: Trichinellosis is a globally distributed food-borne parasitic disease. Initially, diagnosis is usually made based on clinical signs, symptoms, and history of eating raw or undercooked meat. In the present study, an immunochromatographic test (ICT) kit was developed to diagnose trichinellosis by detecting IgG antibodies in sera of infected humans. Methods: Somatic extract from Trichinella spiralis larvae was used as the antigen for the ICT kit development. Diagnostic efficacy was evaluated using human serum samples from proven trichinellosis patients, healthy persons, and those with other parasitic infections. Results: The diagnostic sensitivity, specificity, positive and negative predictive values and accuracy of the ICT kit were 100.0% (95%; CI 88.8 to 100.0%), 92.5% (95% CI; 86.9 to 96.2%), 73.8% (95% CI; 58.0 to 86.1%), 100.0% (95% CI; 97.3 to 100.0%) and 93.8% (95% CI; 89.2 to 96.9%), respectively. Conclusions: This ICT diagnostic kit can be used as a testing tool for human trichinellosis. This test should permit rapid detection of infection to enable prompt anthelminthic treatment. It can also be used for retrospective diagnoses and field surveys based at laboratories where sophisticated equipment is lacking.
- Published
- 2021