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to assess the level of anxiety and characterize the quality of sleep in children living in radioactively con-taminated areas in comparison with children who were not affected by the Chornobyl-affected contingents that werequarantined in connection with the COVID-19 pandemic.The indicators of the level of anxiety were studied using the scale of self-assessment of thelevel of anxiety Ch.D. Spielberger, sleep quality was assessed using a standardized questionnaire for self-completionof PSQI and 137Cs content was measured in children. The main group consisted of 96 children who were quarantineddue to the COVID-19 pandemic and permanently lived in radioactively contaminated areas of Zhytomyr and Rivneregions with a soil contamination density of 137Cs from 18 kBq/m2 to 235 kBq/m2. The age of children ranged from10 to 17 years. Among them were 33 boys and 63 girls. The comparison group consisted of 52 children of similar age,including 26 boys and 26 girls. These children lived permanently in Kyiv and were not victims of the Chornobyl dis-aster.It was found that children who were quarantined for COVID-19 (both residents of radioactively contami-nated areas and children who do not belong to the contingents affected by the Chornobyl disaster) had an increasedlevel of reactive (RA) and personal anxiety (PA). The comparative analysis showed that children of the same sex ofthe main group and the comparison group did not differ in terms of PA and RA. At the same time, studies have shownthat girls, both in the main group and in the comparison group, were characterized by higher levels of PA and RAthan boys. It was determined that poor sleep quality was common in both children living in radioactively contami-nated areas (42.71 %) and children in the comparison group (42.44 %). Among the sleep disorders in children ofboth observation groups, «day dysfunction» was most often detected.There was a direct correlation between the overall PSQI score and the level of reactive, personal anx-iety and the overall PSQI score. Using regression analysis, the presence of a linear association of the level of incorporated 137Cs (Bq) with the indicator of personal anxiety of children living in radioactively contaminated territory (b = -0.716, p0.001) was proved.Meta: otsinyty riven' tryvozhnosti ta okharakteryzuvaty iakist' snu u diteĭ-meshkantsiv radioaktyvno zabrudnenykh terytoriĭ u porivnianni z dit'my, iaki ne nalezhat' do postrazhdalykh vnaslidok Chornobyl's'koï katastrofykontyngentiv, shcho perebuvaly na karantyni z pryvodu pandemiï COVID-19.Materialy i metody. Vyvchalysia pokaznyky rivnia tryvozhnosti za dopomogoiu shkaly samootsinky rivnia tryvozh-nosti Ch.D. Spilbergera, otsiniuvalasia iakist' snu za dopomogoiu standartyzovanogo opytuval'nyka dlia sa-mostiĭnogo zapovnennia PSQI (Pittsburgh Sleep Quality Index) ta provodylos' vymiriuvannia vmistu 137Cs u tiliditeĭ. Osnovnu grupu sklaly 96 diteĭ, iaki perebuvaly na karantyni z pryvodu pandemiï COVID-19 ta postiĭnoprozhyvaly na radioaktyvno zabrudnenykh terytoriiakh Zhytomyrs'koï ta Rivnens'koï oblasteĭ zi shchil'nistiu zab-rudnennia ґruntiv 137Cs vid 18 kBk/m2 do 235 kBk/m2. Vik diteĭ kolyvavsia vid 10 do 17 rokiv. Sered nykh bulo 33khloptsi ta 63 divchyny. Grupu porivniannia sklaly 52 dytyny, analogichnogo viku, sered iakykh bulo 26 khloptsiv ta 26divchat. Tsi dity postiĭno meshkaly u m. Kyievi i ne nalezhaly do postrazhdalykh vnaslidok Chornobyl's'koï kata-strofy. Rezul'taty. Vstanovleno, shcho dity, iaki perebuvaly na karantyni z pryvodu COVID-19 (iak meshkantsi radioaktyv-no zabrudnenykh terytoriĭ, tak i dity, iaki ne nalezhat' do kontyngentiv postrazhdalykh vnaslidok Chornobyl's'-koï katastrofy) maly pidvyshchenyĭ riven' reaktyvnoï ta osobystisnoï tryvozhnosti. Porivnial'nyĭ analiz poka-zav, shcho dity odniieï stati osnovnoï grupy ta grupy porivniannia za pokaznykamy osobystisnoï ta reaktyvnoï (sy-tuatyvnoï) tryvozhnosti ne vidriznialysia. U toĭ zhe chas doslidzhennia pokazaly, shcho divchata, iak v osnovniĭ grupi,tak i v grupi porivniannia, kharakteryzuvalysia bil'sh vysokym rivnem tryvozhnosti, nizh khloptsi. Vyznacheno, shchopogana iakist' snu chasto zustrichalasia iak u diteĭ-meshkantsiv radioaktyvno zabrudnenykh terytoriĭ (42,71 %), taki u diteĭ grupy porivniannia (42,44 %). Sered porushen' snu u diteĭ obokh grup sposterezhennia naĭchastishe vyiav-lialasia «denna dysfunktsiia». Dovedena naiavnist' priamogo koreliatsiĭnogo zv’iazku mizh zagal'noiu otsinkoiu PSQIi rivnem reaktyvnoï, osobystisnoï tryvozhnosti ta zagal'noiu otsinkoiu PSQI. Za dopomogoiu regresiĭnogoanalizu dovedeno naiavnist' liniĭnoï asotsiatsiï rivnia inkorporovanogo 137Cs (Bk) z pokaznykom osobystisnoïtryvozhnosti diteĭ, iaki meshkaiut' na radioaktyvno zabrudneniĭ terytoriï (b = -0,716, p0,001). Vysnovky. Vstanovleno pidvyshchenyĭ riven' reaktyvnoï ta osobystisnoï tryvozhnosti, a takozh poganu iakist'snu z prevaliuvanniam dennoï dysfunktsiï u diteĭ, iaki perebuvaly na karantyni z pryvodu COVID-19. Vyiavlenozalezhnist' vmistu inkorporovanogo 137Cs (Bk) vid rivnia osobystisnoï tryvozhnosti diteĭ, iaki meshkaiut' naradioaktyvno zabrudneniĭ terytoriï.

Behçet's disease (BD) is a systemic vasculitis of unknown origin, characterized by recurrences of the ulcerative process in the oral cavity and on the genitals, inflammatory damage of the eyes, joints, vessels and other organs. The severity and prognosis of BD determines organ pathology. Intestinal manifestations of BD (intestinal BD) are the least studied. Its verification in BD is complicated by the variety of clinical manifestations, their similarity with inflammatory bowel diseases, the lack of informative laboratory tests, pathognomonic endoscopic and histological signs. Intestinal BD can lead to serious complications (massive bleeding, intestinal perforation and fistula formation), which can not only significantly reduce the quality of the patient's life, but also cause death. Treatment of intestinal BD is not standardized; it is mainly empirical and conducted courses. The purpose of therapy is to achieve clinical remission, healing of intestinal ulcers and prevention of surgery. The article presents a case of severe refractory intestinal BD, requiring twice emergency surgical care - removal of half and then the whole of the colon because of multiple perforations. A brief review of the literature is given and diagnostic difficulties of intestinal BD are discussed.Болезнь Бехчета (ББ) - системный васкулит неизвестной этиологии, характеризуется рецидивами язвенного процесса в ротовой полости и гениталиях, поражением глаз, суставов, сосудов и других органов. Тяжесть и прогноз ББ определяет органная патология. Среди последней поражение кишечника наименее изучено. Его верификация при ББ затруднена многообразием клинических проявлений, их схожестью с воспалительными заболеваниями кишечника, отсутствием информативных лабораторных тестов, патогномоничных эндоскопических и гистологических признаков. Поражение кишечника при ББ (ПКББ) может привести к серьезным осложнениям (массивное кровотечение, перфорация кишки и образование свища), что может не только существенно снизить качество жизни пациента, но и стать причиной летального исхода. Лечение ПКББ не стандартизовано, в основном носит эмпирический характер и проводится курсами. Цель терапии - достижение клинической ремиссии, заживление язв кишечника, предотвращения хирургического вмешательства. В статье представлен случай тяжелого рефрактерного поражения кишечника у пациента с ББ, потребовавший дважды неотложной хирургической помощи - удаление половины, а затем и всей толстой кишки из - за множественных перфораций. Приведен кратко обзор литературы и обсуждены диагностические сложности ПКББ.

The article provides a brief overview of the epidemiology of facial nerve neuromas (FNN, schwannomas), its clinical signs, methods of diagnostic and treatment and indications for their use. A rare clinical case of diagnostics, surgical treatment of FNN with intracranial spread and its results are described in detail. The presented case demonstrates a possible low-symptom course of FNN with intracranial spread. The use of a combined surgical approach (translabyrinthine and extradural subtemporal) in conjunction with neurosurgeons makes it possible to remove FNN with intracranial spread with simultaneous intratemporal neuroplasty of the nerve trunk from the tympanic part of the canal to the bottom of the internal auditory canal with a fragment of the sural nerve with an improvement in the function of the affected nerve.В статье представлен краткий обзор эпидемиологии неврином (шванном) лицевого нерва (НЛН), клинических ее проявлений, методов диагностики, способов лечения образования и показания к их применению. Подробно описан редкий клинический случай диагностики, хирургического лечения НЛН с интракраниальным распространением и его результаты. Представленный случай свидетельствует о возможном малосимптомном течении НЛН с интракраниальным распространением. Использование комбинированного хирургического подхода (транслабиринтный и экстрадуральный субтемпоральный) совместно с нейрохирургами позволяет удалить НЛН с интракраниальным распространением с одномоментной интратемпоральной нейропластикой ствола нерва от тимпанальной части канала до дна внутреннего слухового прохода фрагментом икроножного нерва с улучшением функции пораженного нерва.

PROBLEM We hypothesized that expression of transmembrane glycoprotein CD200 on paternal lymphocytes used for pre-gestational lymphocyte immunotherapy (LIT) of recurrent spontaneous abortion (RSA) can suppress the pro-inflammatory Th1-type immunity required for successful implantation. To reveal the association between CD200 expression, female immune background after LIT, and pregnancy establishment, we have performed this work. METHOD OF STUDY Pre-gestational alloimmunizations were given to 37 women using paternal peripheral blood leukocytes, combined with additional alloimmunizations in case of pregnancy. Lymphocyte phenotypes were determined by flow cytometry. Cytokines produced by mitogen-stimulated female peripheral blood cells were estimated by FlowCytomix™ technology. RESULTS We have shown that 78.4% (29/37) of women became pregnant within 12 menstrual cycles after pre-gestational LIT. Pregnancy establishment depends on the intensity of CD200 expression, which is significantly higher on the CD200+ lymphocytes administered to women who later did not achieve pregnancy (P

Seminal plasma (SP) is thought to be a crucial factor which affects the expansion of regulatory T cells (Tregs) in female reproductive tract during embryo implantation. We propose that seminal transforming growth factor (TGF) β1 is responsible for local accumulation of circulating Tregs, which manifests as changes in Treg frequency in peripheral blood, whereas seminal interleukin (IL) 18 interferes with TGF-β1-dependent cellular reactions. The purpose of the present study is to determine whether the frequency of circulating Tregs is associated with the levels of seminal cytokines and pregnancy establishment in women exposed to partner’s SP during in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) cycle. Twenty-nine women were exposed to SP via timed intercourse before the day of ovum pickup (day-OPU) and also subjected to intravaginal SP application just after OPU. Measurements of seminal TGF-β1 and IL-18 were made by FlowCytomix technology. The percentage of CD4+CD25+CD127low+/ – Tregs among total circulating CD4+ T cells was determined by flow cytometry and the difference between Treg values on the day of embryo transfer and day-OPU was calculated. The percentage of Tregs on the day-OPU, identified as a predictive factor of clinical pregnancy after IVF/ICSI, showed a positive correlation with IL-18 concentration and content of this cytokine per ejaculate ( P < .001 and P < .004, respectively) and negative correlation with the TGF-β1/IL-18 ratio ( P < .014).These findings indicate that the adverse effect of seminal IL-18 excess on implantation may be realized by the prevention of postcoital TGF-β1-related migration of circulating Tregs, which clearly manifests as elevated level of Treg frequency in peripheral blood.

The reassortant vaccine strain of live attenuated influenza vaccine inherits temperature sensitivity and areactogenicity from cold-adapted attenuated master donor virus. In Russia, B/ USSR/60/69 master donor virus (B60) is currently in use for the preparation of live attenuated type B influenza vaccine candidates. Trivalent live attenuated influenza vaccine based on A/ Leningrad/134/17/57 and B60 are licensed for the use in Russia for single dose vaccination of adults and children over 3 years. B/Leningrad/14/17/55 (B14) cold-adapted virus is a backup master donor virus for live attenuated type B influenza vaccine. According to our preliminary estimates, it is more attenuated than B60, which can allow expanding applicability of this vaccine for children under 3 years of age. In this paper, the role of B14 genes in its attenuation was assessed. Representative collection of reassortants of B14 with epidemic influenza B viruses was obtained, a phenotypic analysis of reassortants was performed, and their pathogenicity for animals was assessed. The leading role of PB2 and PA genes in attenuation of B14 master donor virus was proven.

The effective application of GIS against parasitic diseases requires the patterns of spread of parasitic diseases in certain natural, climatic, and socioeconomic conditions of different regions where there are epidemically effective carriers. The epidemiological parameters defining a high risk of an epidemiological process after P.vivax importation into Russia were calculated using the developed HealthMapper controlled module with a database. Analysis of the average long-term air temperatures in the administrative territories of Russia over 78 years, namely July, the warmest month of the year when there are the largest numbers of all types of mosquito vectors and high levels of microfilaria in the peripheral blood of in- fected dogs (definitive hosts), has shown that the northern border of the maximum possible area of dirofilariasis in Russia is most fully described by the +14C July isotherm.

Induced malaria continues to be one of the most pressing public health problems in malaria-endemic countries. Ma- laria parasites remain viable in stored blood at a temperature of 2-6°C for 3 weeks. The paper presents current problems associated with transfusion-induced malaria. In the USSR and then in the Russian Federation, sporadic cases of induced malaria (Plasniodium vivax, P.malariae, rarely P.falciparum) were notified (230 cases in 1958 to 1990 and only 5 in 1991 to 2016). Current (immunological and molecular) methods for the laboratory diagnosis of malaria do not provide a 100% detection rMfteor its pathogens; therefore, it is necessary to search for highly efficient, rapid, and low-cost diagnostic methods to ensure the biological safety of donation.

Ménière's disease is a chronic disorder of the inner ear, a permanent sing of which is endolymphatic hydrops. For the diagnosis of Ménière's disease in clinical practice, criteria that are primarily based on the clinical picture. However, instrumental methods for diagnosing Ménière's disease, aimed at identifying endolymphatic hydrops, continue to be developed and improved. Radiation diagnostics contribute significant contribution and aimed at identifying the characteristic signs of Ménière's disease. Recently, it has become possible to visualize endolymphatic hydrops using MRI of the temporal bones. This publication presents an analysis of the current state of this topical aspect of the problem of diagnosing Ménière's disease. The purpose of this review is to conduct a comparative analysis of radiation methods for diagnosing Ménière's disease.The study included publications in English and Russian found in the PubMed, eLibrary database related to the diagnosis of Meniere's disease.A comparative analysis of computed tomography of the temporal bones, magnetic resonance imaging of the temporal bones and diagnostic possibilities for detecting signs characteristic of Ménière's disease are presented. The most diagnostically significant method of studying Ménière's disease is identified.MRI of the temporal bones with contrast enhancement is a promising method that can be used to develop an effective treatment algorithm.Болезнь Меньера — хроническое заболевание внутреннего уха, постоянным признаком которого является эндолимфатический гидропс. Для диагностики болезни Меньера в реальной клинической практике широко используются критерии, которые в первую очередь основаны на клинической картине заболевания. Однако продолжаются разработка и совершенствование инструментальных методов диагностики болезни Меньера, направленных на выявление и количественную оценку эндолимфатического гидропса. Лучевые методы диагностики вносят свой весомый вклад и направлены на выявление специфических признаков болезни Меньера. В последние годы появилась возможность визуализации эндолимфатического гидропса при помощи магнитно-резонансной томографии височных костей. В настоящей публикации представлен анализ современного состояния данного актуального аспекта проблемы диагностики болезни Меньера.Провести сравнительный анализ лучевых методов диагностики болезни Меньера.В исследование включены публикации отечественных и зарубежных авторов о диагностике болезни Меньера, найденные в базах данных PubMed, eLibrary.Проведен сравнительный анализ рентгеновской компьютерной томографии и магнитно-резонансной томографии височных костей и представлены диагностические возможности выявления признаков, характерных для болезни Меньера. Выделен наиболее диагностически значимый метод исследования при данной патологии.Магнитно-резонансная томография височных костей с контрастным усилением является перспективным методом, на основе которого можно разработать эффективный алгоритм лечебной тактики.

It has been proposed that the transforming growth factor (TGF)-β1 present in seminal plasma (SP) triggers a female immune response favorable for implantation. We hypothesize that seminal interleukin (IL)-18, a cytokine that can potentially cause implantation failure, interferes with the beneficial effect of TGF-β1. This study aims to determine whether the levels of seminal TGF-β1 and IL-18 are associated with reproductive outcomes in patients exposed to SP during in vitro fertilization (IVF) or IVF with intracytoplasmic sperm injection (ICSI). A prospective study, which included 71 couples undergoing IVF/ICSI was carried out. Female patients were exposed to their partners' SP via timed intercourse before the day of ovum pick-up (OPU) and also subjected to intravaginal SP application just after OPU. Quantitative measurements of total TGF-β1 (active plus latent) as well as IL-18 were determined by FlowCytomix™ technology in the SP to be used for intravaginal applications. Comparison of SP cytokine profiles between pregnant and non-pregnant groups revealed that pregnancy was correlated with a lower concentration of IL-18 (P=0.018) and lower content per ejaculate for both of IL-18 (P=0.0003) and TGF-β1 (P=0.047). The ratio of TGF-β1-to-IL-18 concentration was significantly higher in the pregnant than in the non-pregnant group (P=0.026). This study supports the notion that two key cytokines TGF-β1 and IL-18, both present in SP are associated with reproductive outcomes in female patients exposed to SP during IVF/ICSI treatment.

The effects of the SH-group blocker N-ethylmaleimide (NEM) and thiol group protector 1,4-dithioerythritol (DTE) on the redox status of cells HBL-100 cells, oxidative modification of their proteins and the state of glutathione and thioredoxin systems have been investigated. Breast epithelial cells cultivated in the presence of NEM were characterized by decreased redox status, increased glutathione reductase activity, and increased concentrations of products of irreversible oxidative modification of protein and amino acids. Cultivation of HBL-100 cells in the presence of DTE resulted in a shift of the redox status towards reduction processes and increased reversible protein modification by glutathionylation. The proposed model of intracellular redox modulation may be used in the development of new therapeutic approaches to treat diseases accompanied by impaired redox homeostasis (e.g. oncologic, inflammatory, cardiovascular and neurodegenerative disease).Issledovali uroven' okislitel'noĭ modifikatsii belkov, sostoianie sistemy glutationa i tioredoksina v usloviiakh moduliatsii redoks-statusa épitelial'nykh kletok molochnoĭ zhelezy linii HBL-100 s pomoshch'iu N-étilmaleimida i 1,4-ditioéritritola. Pri kul'tivirovanii kletok v prisutstvii N-étilmaleimida proiskhodit snizhenie ikh redoks-statusa, uvelichenie aktivnosti glutationreduktazy i povyshenie kontsentratsii produktov neobratimoĭ okislitel'noĭ modifikatsii belkov i aminokislot. Dobavlenie v kul'tural'nuiu sredu protektora SH-grupp sposobstvovalo smeshcheniiu redoks-statusa v storonu vosstanovitel'nykh protsessov i vozrastaniiu obratimoĭ modifikatsii proteinov s pomoshch'iu glutationilirovaniia. Predlozhennaia model' vnutrikletochnoĭ redoks-moduliatsii mozhet byt' ispol'zovana pri razrabotke novykh terapevticheskikh podkhodov dlia lecheniia zabolevaniĭ, soprovozhdaiushchikhsia narusheniem redoks-gomeostaza (onkologicheskikh, vospalitel'nykh, serdechno-sosudistykh, neĭro-degenerativnykh i dr.).

Seventeen 1,4-benzoxazin-2-ones bearing the enaminone moiety and three of their analogs were tested for the antibacterial activity against Mycobacterium tuberculosis (H37Rv). Minimal bactericidal concentrations (MBCs) were determined after 41 days of incubation by BACTEC. 1,4-Benzoxazin-2-ones bearing the unsubstituted benzo moiety showed the most promising activities (MBC = 5.00 µg/ml). For most active compounds, antibacterial activities were determined daily during the 41 days. The most promising compound showed a bacteriostatic effect at a concentration of 0.31 µg/ml on Day 4 of incubation, 0.62 µg/ml on Day 6, 2.50 µg/ml on Day 9, and 5.00 µg/ml on Day 41. All studied compounds, along with some of their reported analogs, were docked to 35 proteins of M. tuberculosis to find their potent targets in these organisms. As a result of reverse docking, aspartate 1-decarboxylase, panD, was selected as the most appropriate target. Docking of the most active compounds to mutant panD from pyrazinamide-resistant strains of M. tuberculosis implies that they would not be active against these strains. Considering that most of pyrazinamide clinical resistance cases are due to loss-of-function mutations in pyrazinamidase, pncA, compounds from this study could be useful drugs for the treatment of some cases of pyrazinamide-resistant tuberculosis.

A larger number of publications on cases of. complicated vivax malaria in the world literature can be. explained by a set of diffeetit faciors'. There has been unification of the definition of complicated tertian malaria allowing ihe clinical nianagement of a patient and the reporting of complicated cases of P.vivax to be improved. The, epidemiological characteristics of complicated vivax malaria are determined by a diversity of geographical, races, variants, gentyies and phenotypes of tertian malaia due to intensive internal and external migration. Chloroquine resistance and primaquine refractoriness contribute to increased local malaria transmission due to a rise in cases of reinfection during-epidemics of vivax malaria. From an epidemiological point of view, the increasing number of complicated vivax malaria cases indicates the untimely detection and treatment of malaria patients, the low level of medical personnel training and a negative attitude of the local population towards the recommendations of national malaria programs for prevention measures. Unsolved problems can substantially retard the time to achieve malaria elimination.

The paper describes a case of the rare localization of a pregnant Dirofilaria female in a man's genitalia. Patient T born in 1973 was infected in the Krasnoyarsk Territory presumably in the summer of 2012. A migratory mass appeared under the skin of the chest at the beginning of 2016. A dense mass was located beneath the skin in the penis by early April. Histological specimens from the removed tumor, entered the specialized Clinical Diagnostic Laboratory for parasitic diseases. The specimens exhibited transverse and oblique sections of Dirofilaria spp. The body cavity contained nematodes - multiple microfilariae. However, routine studies and PCR could not reveal the microfilariae in the blood.

We studied the constellation of genes encoding polymerase complex proteins of master donor viruses for Russian live attenuated influenza vaccine type B. Reassortants of the reserve attenuation donor B/Leningrad/14/17/55 with B/USSR/60/69 master donor virus currently used for manufacturing seasonal influenza vaccine were prepared and examined. Most reassortants obtained by the classical reassortment method inherited all genes from the B/Leningrad/14/17/55 virus except the gene encoding PB1 subunit of the polymerase complex. One reassortant was selected for further evaluation of the role of PB1 gene. Greater attenuation of the strain for experimental animals (mice) in comparison with the original strains was demonstrated. This indicates high degree of constellation of genes of cold-adapted master donor viruses and the important compensating role of amino acid substitutions in the PB1 protein of B/Leningrad/14/17/55 donor virus in preventing viral hyperattenuation.

We compared three cold-adapted live attenuated influenza vaccine strains prepared by reverse genetics methods on the basis of master donor virus A/Leningrad/134/17/57 and influenza H7N9 strains A/Anhui/1/2013 and A/Shanghai/1/2013. Two strains based on A/Anhui/1/2013 differed by amino acid positions 123 and 149 in HA1 (123N/149N; 123D/149D). All strains efficiently replicated in developing chicken embryos; A/Shanghai/1/2013-based strain and A/Anhui/1/2013-123N/149N variant were characterized by reduced replication in MDCK cells. Strains based on A/Anhui/1/2013 virus agglutinated erythrocytes with α2,3- and α2,6-linked sialic acid residues, whereas strain A/Shanghai/1/2013 only α2,3. In experiments with BALB/c mice, Anhui-123D/149D strain was most immunogenic and induced high crossreactive humoral immune response, therefore it can be recommended as the model virus for the construction of recombinant vector vaccines based on live attenuated influenza vaccine.

To determine age-relatedpeculiarities of trace element system in oldpeople, residing in different natural and geographical regions, the content of 25 trace elements in hair samples of women from Arkhangelsk (average age is 87,0±0,98 years) and Magadan (average age is 80,1±1,25 years) was determined by spectrometric methods. In both groups there was established element misbalance characterized by lower concentration of such essential elements as Ca, Mg, Co, Cu and higher concentration of Na. The excess of K and Zn is more expressed in hair of female residents of Arkhangelsk in comparison with the city of Magadan there was revealed significantly more higher content of aluminum, boron, silicon, in the city of Magadan there are significantly higher concentrations of selenium and tin. According to the frequency of occurrence the excess of elements in the body of residents of the city of Arkhangelsk is comparable to that of residents of the city of Magadan, but has its own features. In Arkhangelsk there is more pronounced the excess ofpotassium, there was found high content of zinc, whereas in the city of Magadan there was revealed an excess of manganese and arsenic. Square of the figure, reflecting the deficiency of elements in female residents of the city of Magadan is more than in the city of Arkhangelsk, which is manifested by larger deficiency of chromium, zinc, iron. Identified regional differences in the content of macro- and microelements are related to biogeochemical and ecological features of the regions, which can determine the specifics of the aging diseases and time of their occurrence.

Activity of serum antibodies in vitro binding to endothelial cells in women with normal pregnancy and preeclampsia was studied. Flow cytometry detected peculiarities of antibody binding to endothelial cells in health and disease. Detection of antiendothelial antibodies in trimesters II and III can be diagnostically important in preeclampsia.

The aim of the study was to assess the results of surgical treatment in 74 patients with extensive arteriovenous angiodisplasia in the head and neck area. All patients underwent complex examination including ultrasound, CT and MRI with contrast and selective carotid angiography. The lesions excision was performed after endovascular embolization and soft tissue defects were restored by various methods selected according to prevalent vascular lesion type (arterial, arteriovenous, venous, capillary) and blood flow values (high flow, low flow, mixed). Good and satisfactory esthetic results were seen in 95.2% of cases, lesion relapse was detected in 4.8% of cases by 12-18 months follow-up. Endovascular embolization proved to decrease the intraoperative bleeding risk, prevent lesion relapse and minimize the size of the lesion improving the results of radical treatment. Local flap reconstruction usually results in good esthetic results. By local soft tissue deficiency distant free flaps or microsurgical complex flaps may be used.Цель работы - оценка результатов лечения больных с обширными артериовенозными ангиодисплазиями в области головы и шеи. Наблюдали 74 случая обширных артериовенозных ангиодисплазий в области головы и шеи. Проводилось комплексное диагностическое обследование, включавшее ультразвуковое исследование, компьютерную и (или) магнитно-резонансную томографию с контрастированием, селективную каротидную ангиографию. После эндоваскулярной эмболизации удаляли образование с одномоментной пластикой мягкотканного дефекта по различным методикам. Необходимый вариант лечения выбирали на основании определения преимущественного типа строения ангиодисплазии (артериальный, артериовенозный, венозный, капиллярный), а также скоростных характеристик (высокоскоростной, низкоскоростной, смешанный). Хороший или удовлетворительный результат отмечен в 95,2% наблюдений, рецидивы заболевания в срок 12-18 мес наблюдали в 4,8% наблюдений. Эндоваскулярная эмболизация в качестве первого этапа лечения позволяет снизить риск кровотечения при выполнении следующего этапа хирургического лечения, является методом профилактики рецидива заболевания, уменьшает объем ангиодисплазии, тем самым расширяются возможности радикального лечения. Использование пластики местными тканями позволяет добиться хороших эстетических результатов. При дефиците местных тканей следует использовать лоскуты из отдаленных участков, либо микрохирургическую аутотрансплантацию комплексов тканей.

Heat shock proteins Hsp) act as molecular chaperones, protecting enzymes and other proteins against reactive oxygen species. The objective of the study was to investigate the role of Hsp27 in maintaining the balance of the glutathione system and Hsp70 concentrations as well as in implementing Jurkat tumor cell apoptosis. Addition of the Hsp27 inhibitor KRIBB3 (5-(5-ethyl-2-hydroxy-4-methoxyphenyl)-4-(4-methoxyphenyl)-isoxazol) to Jurkat cells resulted in glutathione redox imbalance (increased GSSG and increased glutathione reductase activity), a decrease in Hsp70 concentrations, and also increased cell apoptosis as compared with to the intact cell culture. The proposed selective regulation of chaperone activity is a promising direction in regulating apoptosis at the cellular level.Rassmotreny vozmozhnosti upravleniia apoptoticheskoĭ gibel'iu opukholevykh kletok linii Jurkat s pomoshch'iu regulirovaniia refoldinga belkov. Belki teplovogo shoka igraiut rol' molekuliarnykh shaperonov, zashchishchaia fermenty i drugie belki ot vozdeĭstviia aktivnykh form kisloroda, krome étogo iavliaiutsia anti- i proapoptogennymi faktorami. Tsel'iu dannogo issledovaniia bylo ustanovit' rol' Hsp27 v podderzhanii sostoianiia sistemy glutationa, soderzhaniia Hsp70 i realizatsii apoptoza opukholevykh kletkakh linii Jurkat. Pri dobavlenii v sredu kul'tivirovaniia opukholevykh kletok linii Jurkat ingibitora Hsp27 KRIBB3 (5-(5-étil-2-gidroksi-4-metoksifenil)-4-(4-metoksifeni)-izoksazol) ustanovleny snizhenie kontsentratsii Hsp70, disbalans sistemy glutationa (uvelichenie kontsentratsii okislennogo glutationa i aktivnosti glutationreduktazy), proiskhodiashchie na fone aktivatsii apoptoticheskoĭ gibeli kletok po sravneniiu s intaktnoĭ kul'turoĭ étikh kletok. Predlozhennoe selektivnoe upravlenie aktivnost'iu shaperonov predstavliaet soboĭ perspektivnoe napravlenie reguliatsii apoptoza na kletochnom urovne.

Formation of labyrinth fistulas and destruction of the facial nerve canal in children with middle ear cholesteatoma is rare. Data of children with cholesteatoma of the middle ear, operated in the ENT department of MONIKI, is analyzed. Several clinical observations of multiple-aged patients with the presence of cholesteatoma intra-temporal complications are presented. The preoperative computer tomography made it possible to diagnose the presence of the labyrinth fistula and the destruction of the facial nerve channel, which was confirmed intraoperatively.Формирование фистул лабиринта и деструкция канала лицевого нерва у детей с холестеатомой среднего уха встречается нечасто. Проанализированы данные детей с холестеатомами среднего уха, прооперированных в ЛОР-отделении МОНИКИ. Представлены несколько клинических наблюдений пациентов различного возраста с наличием интратемпоральных осложнений холестеатомы. Проведенная дооперационная компьютерная томография позволила диагностировать наличие фистул лабиринта и деструкцию канала лицевого нерва, что было подтверждено интраоперационно.

By methods of atom-emission and mass spectrometry with inductively bonded argon plasma there was determined the content of 25 macro- and trace elements in tap cold drinking water used by the residents of the city of Magadan for food purposes and in hair samples of 30 young male Europeans aged of 17-23 years, who are the residents, of the city of Magadan. According to our data the content of 25 elements in drinking water conforms to standards, but that content of such essential elements as Co, Cr Cu, I, Mn, Na, Se, Zn is shown to be lower than referential indices. After boiling the water the concentration of trace elements is changed. The content of Cd, Cu, K, P Pb, Zn, Ni becomes lower significantly. In healthy young men aged of 17-23 years, from the number of natives Europeoids, residents of the North there was detected deficit of Co and I (86% and 62%, respectively), lower concentrations of Ca, Mg, Se, Zn (76%, 69%, 24%, 24%, respectively). The constant use by residents of the city of Magadan of ultrafresh brackish drinking water in food aims may be the one of the main reasons of the imbalance of macro- and micronutrients in the body, characterized by features of the so-called "northern" type with a marked deficiency of basic essential elements.

In order to study the body nutrition supply in 17-37 year-old Magadan residents, among them Europeans (control group, n = 200) and Indigenous Minorities of the North (IMN, n = 56), we examined hair samples of the subjects and ascertained the content of 25 essential minerals and trace elements using the method of atom-emission and mass spectrometry with the inductively bonded argon plasma. At the same time, these elements were detected un drinking water and in local food products including samples of muscles and milt (testes) of salmon (Salmoidae), fished out in the Okhotsk Sea near shore. When comparing the values of the examined male Europeans and subjects IMN, the latter demonstrated reliably higher Ca (373.37 mcg/g vs. 256.72 mcg/g), Mg (34.09 mcg/g vs. 24.89 mcg/g), P (184.30 mcg/g vs. 157.60 mcg/g), I (1.13 mcg/g vs. 0.50 mcg/g); in the examined females--Mg(56.66 mcg/g vs. 32.30 mcg/g) and P (181.35 mcg/g vs. 149.40 mcg/g). Besides, the IMN examinees, both males and females, demonstrated reliably lower Fe values in comparison with the control group. When comparing male and female subjects, we found the first ones to be reliably higher in As, Cr, K, Na, I, Cd and Li but lower in Ca, Co, Mg and Mn. The present study provides data on the content of minerals and trace elements in some food products, widely represented in food ration of the residents of Magadan town. Interspecies differences in the content of chemicals also conformed to the maximum permissible concentration approved for food products, but Co, Cu, Na and Zn values in Chum salmon proved to be reliably higher than those in Coho salmon. Fe levels being within 3.8-4.9 mcg/g were practically similar to fresh-water fish. The contents of K and P in the examined fish species were found as rather significant (3448-8879 mcg/g and 2795-3535 mcg/g, respectively).

The paper shows that there are more seropositive people in a group of those who have experienced road accidents (RA) and who are responsible for the latter than in a control group. The investigations have indicated that chronic toxoplasmosis may be regarded as a potential increased risk factor of RA.

Riboflavin (Rf) is a vitamin and endogenous photosensitizer capable to generate reactive oxygen species (ROS) under UV-blue irradiation and kill cancer cells, which are characterized by the enhanced uptake of Rf. We confirmed its phototoxicity on human breast adenocarcinoma cells SK-BR-3 preincubated with 30-μM Rf and irradiated with ultraviolet light, and proved that such Rf concentrations (60 μM) are attainable in vivo in tumour site by systemic intravascular injection. In order to extend the Rf photosensitization depth in cancer tissue to 6 mm in depth, we purpose-designed core/shell upconversion nanoparticles (UCNPs, NaYF4:Yb3+:Tm3+/NaYF4) capable to convert 2% of the deeply-penetrating excitation at 975 nm to ultraviolet-blue power. This power was expended to photosensitise Rf and kill SK-BR-3 cells preincubated with UCNPs and Rf, where the UCNP-Rf energy transfer was photon-mediated with ~14% Förster process contribution. SK-BR-3 xenograft regression in mice was observed for 50 days, following the Rf-UCNPs peritumoural injection and near-infrared light photodynamic treatment of the lesions.

The level of antipaternal antileukocytic antibodies detected by flow cytometry is a parameter reflecting the efficiency of alloimmunization of women with reproductive disorders during preparation to pregnancy. The results of evaluation of antipaternal antileukocytic antibodies by two modifications of the method are presented. The optimal method for detection of antipaternal antileukocytic antibodies after immunocytotherapy is selected.

The concept of 'vasculogenic mimicry' (VM) was introduced to describe the unique ability of highly invasive tumor cells to form capillary-like structures (CLS) and matrix-rich patterned network in three-dimensional culture that mimic embryonic vasculogenic network. Recently, we have shown that CLS formation requires apoptotic cell death through activation of caspase-3-dependent mechanism. In this study, to identify some molecular determinants driving aggressive melanoma cells to express a latent 'angiogenic program' that recapitulates the early events of CLS formation, we focused on the involvement of antioxidants (AOs) in the process of melanoma VM. We have studied the effects of resveratrol, (-)-epigallocathechin gallate, N-acetyl-cysteine (NAC) and Trolox on the ability of melanoma cells to form/destroy CLS. We observed that the formation of CLS was strongly related to reactive oxygen species level. In vivo animal experiments confirmed the involvement of reactive oxygen species level in melanoma VM. To understand the molecular mechanisms of this phenomenon, we specifically looked for induction of apoptosis and vascular endothelial growth factor (VEGF) release. Western blot analysis revealed that the level of VEGF, VEGF receptors (VEGF-Rs) and active caspase-3 dramatically decreased in cells treated with AOs. Here, we also report further experiments designed to determine whether the crosstalk between AOs and apoptosis exists in melanoma VM.

A case of primary infectious endocarditis with the lesion of mitral valve in a pregnant woman is reported The diseases was caused by meticillin-resistant Staphylococcus aureus. Special attention is given to inefficiency of beta-lactame antibiotics against this infection and beneficial effect of daptomycin therapy. This observation confirms literature data about high frequency of thromboembolic complications of S aureus-induced infectious endocarditis due to the production of various coagulases and von Willebrand factor-binding protein by these microorganisms. An increase of coagulation caused by S. aureus is mediated through activation of prothrombin, factor XIII, and fibrin-binding fibronectin. It requires prescription of direct thrombin inhibitor pradax that proved to yield good results in the treatment of our patient. It is concluded that infectious endocarditis in pregnant women is characterized by an atypical clinical picture due to impaired immunity associated with rapid progression of the process after delivery, high frequency of thromboembolic and DIC syndromes.

Malaria vigilance is a continuation of the functions of the malaria surveillance system in the countries that have achieved malaria elimination on their territories. The main function of the malaria vigilance during the post-elimination period is a continuous monitoring and evaluation and implementation of necessary prevention measures against the possible re-establishment of local malaria transmission due to the importation of malaria from its endemic countries by residents and/or migrants. Various epidemiological aspects of malaria vigilance, such as standard identification of a malaria case during the post-elimination period; notification procedures and reporting systems; training of personnel; free access to malaria diagnosis and treatment; mandatory laboratory confirmation of malaria diagnosis; and prompt efficacious treatment, are discussed. Other aspects include epidemiological classification of cases, seasonal pattern of imported cases, its territorial confinement, time-lag between the detection of a case, its diagnosis and treatment initiation, evaluation of the efficacy of individual chemoprophylaxis and treatment.

The review of the literature about the results of the study of the role of 4a/b VNTR-polymorphism of eNOS in pathophysiology of various states of the body. It is shown that the data are ambiguous and sometimes contradictory. The study of the contribution of candidate genes to the implementation of multifactorial diseases can increase the accuracy of prediction of the set of risk factors, early diagnosis facilitate and sufficient therapy of multifactorial diseases.

The authors consider in brief epidemiology, diagnostics, and variants of the surgical treatment of the rare clinical condition pneumocele and pneumoencephalocele of the temporal bone. Diagnostic and surgical modalities for the management of multiple pneumocele associated with temporal bone hyperpneumatization are discussed.Кратко представлены эпидемиология, диагностика и варианты хирургического лечения редко встречающегося заболевания - пневмоцеле и пневмоэнцефалоцеле височной кости. Описаны диагностика и хирургическое лечение множественного пневмоцеле при гиперпневматизации височной кости.

The review presents the results of trials of the clinical efficacy of a test antimalarial drug for each malarial parasite species, which were published in 2000-2013 and supplemented by the data of in vitro studies or investigations using the molecular markers of resistance. There are data on the resistance of each medicament since many of the drugs are used in combination with artermisinin derivatives.

We applied scanning electron microscope to study of surface architectonics of erythrocytes and lymphocytes peripheral blood in children born after the Chernobyl accident and living in conditions of chronic incorporation 137Cs. We found significant changes in surface structure membranes of red blood cells and peripheral blood lymphocytes in the basic childrens group compared with control one. The most striking changes were in children with levels incorporated 137Cs from 6845 to 16522 Bq.

The ultrastructure of peripheral blood lymphocytes of children which living in conditions of chronic intake of 137Cs through the food chain studied by method of transmission electron microscopy. The essential changes in the submicroscopic organization of intracellular organelles, especially mitochondria, in the basic group compared to control. The most pronounced changes were observed in the subgroup of children with the level of incorporated 137Cs exceeding 6845 Bq.

Background. The term “paraduodenal pancreatitis” (PP) was proposed as a synonym for duodenal dystrophy (DD) and groove pancreatitis, but it is still unclear what organ PP originates from and how to treat it properly.Objective. To assess the results of different types of treatment for PP.Method. Prospective analysis of 62 cases of PP (2004–2013) with histopathology of 40 specimens was performed; clinical presentation was assessed and the results of treatment were recorded. Results. Preoperative diagnosis was correct in all the cases except one (1.9%). Patients presented with abdominal pain (100%), weight loss (76%), vomiting (30%), and jaundice (18%). CT, MRI, and endoUS were the most useful diagnostic modalities. Ten patients were treated conservatively, 24 underwent pancreaticoduodenectomies (PD), pancreatico- and cystoenterostomies (8), Nakao procedures (5), duodenum-preserving pancreatic head resections (5), and 10 pancreas-preserving duodenal resections (PPDR) without mortality. Full pain control was achieved after PPRDs in 83%, after PDs in 85%, and after PPPH resections and draining procedures in 18% of cases. Diabetes mellitus developed thrice after PD.Conclusions. PD is the main surgical option for PP treatment at present; early diagnosis makes PPDR the treatment of choice for PP; efficacy of PPDR for DD treatment provides proof that so-called PP is an entity of duodenal, but not “paraduodenal,” origin.

The experience of the countries who have achieved malaria elimination on their territories suggests that the main tasks of a malaria program in the post-elimination period are as follows: to maintain healthcare workers' alertness for possible malaria importation from outside and for a risk of its renewed transmission in the malaria-free area; to preserve the medical staff and to improve its professional skills in malaria diagnosis, treatment, and prevention in both the public and private sectors; to maintain malaria alertness among the population, among higher-risk population groups in particular; to elaborate and implement an effective program for collaboration with neighboring malaria-endemic countries, in the boundary regions in particular. The national governments' determination to continue long-term financial support of the prevention program of malaria in its elimination period is the guarantee of successful solution of the tasks set.

Pigment epithelium-derived factor (PEDF), a 50 kDa secreted glycoprotein, is among the most potent endogenous inhibitors of angiogenesis. PEDF-derived fragment (44-77) possesses antiangiogenic properties of the full-sized protein and is a potential drug candidate for the treatment of ocular neovascular diseases. In this study we propose an efficient scalable biotechnological method for the production of PEDF (44-77) as part of a fusion protein with SspDnaB intein. The fusion protein was obtained in bacterial E. coli cells in the form of inclusion bodies, solubilized and subjected to autocatalytic cleavage with the release of PEDF (44-77) (yield, 77%). The target peptide was separated from the intein using tangential ultrafiltration. The final purification of PEDF (44-77) was performed by reversed-phase HPLC. The yield of the target peptide (purity, 99%) was 65 mg per 1 liter of culture. Antiangiogenic activity of the obtained peptide was studied in vitro using murine endothelial cells SVEC-4-10. PEDF (44-77) suppressed proliferation of endothelial cells by 53% and inhibited endothelial cell tube formation at the concentration of 1 nM. The ability of the recombinant PEDF (44-77) to block initial stages of angiogenesis was demonstrated using the model of rabbit corneal neovascularization.

Effects of ionizing radiation registered in cells after low dose irradiation are still poorly understood. A pulsed mode of irradiation is even more problematic in terms of predicting the radiation-induced response in cells. Thus, the aim of this paper was to study and analyze the effects of dose and frequency of pulsed X-rays on the frequency of radiation-induced DNA double-strand breaks and their repair kinetics in human peripheral blood lymphocytes in vitro. Analysis of radiation-induced gammaH2AX and 53BP1 repair foci was used to assess the DNA damage in these cells. The dose-response curve of radiation-induced foci of both proteins has shown deviations from linearity to a higher effect in the 12-32 mGy dose range and a lower effect at 72 mGy. The dose-response curve was linear at doses higher than 100 mGy. The number of radiation-induced gammaH2AX and 53BP1 foci depended on the frequency of X-ray pulses: the highest effect was registered at 13 pulses per second. Moreover, slower repair kinetics was observed for those foci induced by very low doses with a nonlinear dose-response relationship.

This communication is the first in the series consisting of two publications describing the present state of malaria control and elimination in the world. The global malaria situation in 2009-2010 demonstrated a considerable situation as compared to the previous years. This improvement is associated with a considerable global increase of investments made by both national governments and world society to the malaria control programs. Spectacular progress has been achieved even in the areas of the most infection-affected African countries situated to the south of the Sahara Desert. It has been estimated that malaria cases in the world declined from 233, 000,000 in 2000 to 225,000,000 in 2009. Malaria mortality decreased from 985,000 in 2000 to 781,000 in 2009. To maintain the results achieved and to further reduce the problem of malaria worldwide, it is necessary to ensure a long-term political and financial support for malaria control programs at the national and international levels.

The state-of-the-art of malaria elimination programs worldwide and the advances made in the past decade were analyzed and assessed. The factors contributing to or reducing the achievement of the final objective of malaria elimination--to interrupt the local transmission of the infection and to steadily maintain the achieved successes are given. Technical problems are defined at the final stages of a malaria elimination program. The problems include difficulties in identifying patients and asymptomatic parasite carriers due to the low level of malaria transmission and the absence of highly effective methods for detecting malaria parasites with low parasitemia, as well as mixed malaria infections requiring the use of different treatment regimens and antimalarial drugs. Large-scale uncontrolled population migration is noted to be of importance in spreading the infection in a malaria-free area. The solution of urgent tasks is to improve the existing methods and develop new ones for the detection and treatment of the infection and a package of antimalarial measures.

Twenty-three serum samples from 12 patients with Echinococcus alveolaris collected 1 to 12 years after surgery and 1--13 courses of specific therapy were used to evaluate the diagnostic effectiveness of an enzyme immunoassay (EIA) test system with cystic Echinococcus antigen. The findings enable the EIA test system with cystic Echinococcus antigen to be recommended for the diagnosis of Echinococcosis alveolaris and for the monitoring of its treatment results.

This review deals with the influence of secondary pathogenic factors that include impaired circulatory autoregulation, altered axonal transport, a glutamate calcium cascade of reactions, reduced natural antioxidant system, and accumulated lipid peroxidation products in the eye tissues, pseudoexfoliation syndrome, diminished ciliary muscle function on the pathogenesis of primary open-angle glaucoma. The above factors serve as an element of the major pathogenic factors (elevated intraocular pressure, hemodynamic disorders), aggravate the course of a glaucomatous process and should be kept in mind when complex treatment for the disease is used.

Studies to design a dry chromogenic nutrient medium for the diferentiation of Klebsiella were under way, by detecting the intracellular Klebsiella genus-specific enzyme of human potential pathogenicity--5-aminosalicylate decarboxylase. The composition of the proposed medium that ensured its high sensitivity and improved its differentiating properties as compared with the known traditional media was worked through. Klebsiella are isolated and identified on the proposed medium in one step, which substantially reduces diagnosis time and material costs and takes some burden from microbiologists.

The paper deals with the topical problem of modern gastroenterology and coloproctology--the influence of genetic factors on the development and course of Crohn's disease (CD). It presents the data of foreign authors and the results of Russia 's first study evaluating the effect of the major polymorphic variants of the NOD2/CARD15 gene predisposing to CD on the risk, course, and other features of CD.

A case of Kawasaki disease is reported with manifestations of autoimmune vasculitis and predominant affection of coronary arteries. The relevant literature review describes major symptoms of this pathology and risk factors of its atypical forms. It is emphasized that rare occurrence of Kawasaki disease among non-mongoloid subjects should not make the general practitioner les suspicious of this condition.

The authors describe the state-of-the-art of gastric cancer detection in detail and show the current principles of its radiodiagnosis. They provide a detailed explanation for that radiodiagnosis should be urgently returned into gastroenterology as an equal with endoscopy, which is based on the existing tendency for the major morphological types of gastric cancer to appear. The present-day capacities of radiodiagnosis of gastric cancer are mainly based on the introduction of the digital technologies into traditional radiology, which will replace the analog imaging principle in the foreseeable future. The authors present their data of examining 300 gastric cancer cases diagnosed by the CR system and later verified by a morphological study of resected gastric specimens. They emphasize that intraparietally growing gastric cancer is frequently inaccessible for endoscopy long, which determines the need for two basic procedures (radiodiagnosis and endoscopy) coexisting on a par for the detection of gastric cancer. They are sure that computed radiology (CR) will be the most acceptable way of changing traditional radiology to the digital imaging principle in primary, municipal health care in the near future.

Somatic and genetic effects at children who have undergone the influence of ionizing radiation during in utero development and at the subsequent stages ontogenesis are investigated, in view of dozes of the general irradiation and equivalent dozes of an irradiation of red bone brain. 1144 children were examined: 1st group consisted of children who have been born from women pregnant at the moment of failure, evacuated from Pripyat; 2nd group included children who have been born from women pregnant at the moment of failure, stayed to live in a zone of the strict radiation control; 3rd group was the control group--children who have been born in 1986, living in safe region of the Ukraine according to radiation conditions. The presence of direct correlation link between quantity of small anomalies of the development and the total radiation dose of the fetus (R = 0.61, p0.002) and inverse link with fetus age at the moment of radiation factor effect (R = -0.53, p0.003) has been established. Significant correlation links between the total radiation dose and the level of children health (R = 0.45, p0.03); the equivalent radiation dose of the red bone marrow and the frequency of damages in the chromosomal apparatus of the somatic cells (1st group--R = 0.51, p0.02; 2nd group--R = 0.62, p0.002) have been revealed.