1. Polymorphonuclear Leukocytes Activation: In Vitro Modulation by 6-Methoxy-2-Naphthylacetic Acid, The Major Active Metabolite of Nabumetone
- Author
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M Peroni, C. Cervini, A. Allegrezza-Giulietti, and R. Serretti
- Subjects
Neutrophils ,Nabumetone ,Metabolite ,In Vitro Techniques ,Pertussis toxin ,Naphthaleneacetic Acids ,Acetic acid ,chemistry.chemical_compound ,medicine ,Humans ,NADH, NADPH Oxidoreductases ,Virulence Factors, Bordetella ,Active metabolite ,Pharmacology ,chemistry.chemical_classification ,Reactive oxygen species ,NADPH oxidase ,biology ,Anti-Inflammatory Agents, Non-Steroidal ,Zymosan ,NADPH Oxidases ,Free Radical Scavengers ,Butanones ,Pertussis Toxin ,chemistry ,Biochemistry ,Luminescent Measurements ,biology.protein ,medicine.drug - Abstract
Reported here are the results of a study carried out in order to assess the possible influence of the major circulating active metabolite of nabumetone, 6-methoxy-2-naphthylacetic acid, on some aspects of polymorphonuclear leukocytes activation. The action of 6-methoxy-2-naphthylacetic acid was tested on the following features of polymorphonuclear leukocytes activation: reactive oxygen species production in polymorphonuclear leukocytes stimulated with different agonists, reactive oxygen species production in polymorphonuclear leukocytes treated with pertussis toxin, NADPH-oxidase activity. The findings showed that 6-methoxy-2-naphthylacetic acid decreased reactive oxygen species production in polymorphonuclear leukocytes stimulated with N-formyl-methionyl-leucyl-phenylalanine, phorbol myristate acetate, and opsonized zymosan, but failed to provoke a decrease of reactive oxygen species production when the cells were stimulated with calcium ionophore A23187. Moreover, 6-methoxy-2-naphthylacetic acid did not influence the transduction of the signal following the binding of stimuli to membrane receptors, and NADPH-oxidase activity. Finally, our findings showed that 6-methoxy-2-naphthyl acetic acid had no scavenger effect on reactive oxygen species production.
- Published
- 1993