8 results on '"Asquith, Becca"'
Search Results
2. Quantification of HTLV-1 Clonality and TCR Diversity.
- Author
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Laydon, Daniel J., Melamed, Anat, Sim, Aaron, Gillet, Nicolas A., Sim, Kathleen, Darko, Sam, Kroll, J. Simon, Douek, Daniel C., Price, David A., Bangham, Charles R. M., and Asquith, Becca
- Subjects
HTLV-I ,T cell receptors ,IMMUNOLOGY ,MICROBIAL diversity ,CLONE cells - Abstract
Estimation of immunological and microbiological diversity is vital to our understanding of infection and the immune response. For instance, what is the diversity of the T cell repertoire? These questions are partially addressed by high-throughput sequencing techniques that enable identification of immunological and microbiological “species” in a sample. Estimators of the number of unseen species are needed to estimate population diversity from sample diversity. Here we test five widely used non-parametric estimators, and develop and validate a novel method, DivE, to estimate species richness and distribution. We used three independent datasets: (i) viral populations from subjects infected with human T-lymphotropic virus type 1; (ii) T cell antigen receptor clonotype repertoires; and (iii) microbial data from infant faecal samples. When applied to datasets with rarefaction curves that did not plateau, existing estimators systematically increased with sample size. In contrast, DivE consistently and accurately estimated diversity for all datasets. We identify conditions that limit the application of DivE. We also show that DivE can be used to accurately estimate the underlying population frequency distribution. We have developed a novel method that is significantly more accurate than commonly used biodiversity estimators in microbiological and immunological populations. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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3. Quantifying HTLV-I dynamics.
- Author
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Asquith, Becca and Bangham, Charles R. M.
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IMMUNE response , *VIRUSES , *T cells , *HTLV-I , *MATHEMATICAL models , *INFECTION - Abstract
Despite significant advances in our understanding of the immune response to persistent viruses like human T-cell lymphotropic virus type I (HTLV-I), many important questions remain unanswered. Mathematical modelling enables us to interpret and synthesise diverse experimental data in new ways and thus can contribute to our understanding. Here, we review recent advances in mathematical modelling of HTLV-I infection and illustrate how mathematics has enabled us to identify factors that determine an individual's viral burden and risk of developing HTLV-I-associated diseases.Immunology and Cell Biology (2007) 85, 280–286; doi:10.1038/sj.icb.7100050; published online 20 March 2007 [ABSTRACT FROM AUTHOR]
- Published
- 2007
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4. In vivo T lymphocyte dynamics in humans and the impact of human T-lymphotropic virus 1 infection.
- Author
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Asquith, Becca, Yan Zhang, Mosley, Angelina J., de Lara, Catherine M., Wallace, Diana L., Worth, Andrew, Kaftantzi, Lambrini, Meekings, Kiran, Griffin, George E., Yuetsu Tanaka, Toughs, David F., Beverley, Peter C., Taylor, Graham P., Macallan, Derek C., and Bangham, Charles R. M.
- Subjects
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T cells , *HTLV-I , *CD4 antigen , *HTLV-I infections , *CELL proliferation , *GENE expression , *RETROVIRUSES - Abstract
Human T-lymphotropic virus type 1 (HTLV-1) is a persistent CD4+ T-lymphotropic retrovirus. Most HTLV-1-infected individuals remain asymptomatic, but a proportion develop adult T cell leukemia or inflammatory disease. It is not fully understood how HTLV-1 persists despite a strong immune response or what determines the risk of HTLV-1-associated diseases. Until recently, it has been difficult to quantify lymphocyte kinetics in humans in vivo. Here, we used deuterated glucose labeling to quantify in vivo lymphocyte dynamics in HTLV-1-infected individuals. We then used these results to address four questions. (i) What is the impact of HTLV-1 infection on lymphocyte dynamics? (ii) How does HTLV-1 persist? (iii) What is the extent of HTLV-1 expression in vivo? (iv) What features of lymphocyte kinetics are associated with HTLV-1-associated myelopathy/tropical spastic paraparesis? We found that CD4+CD45RO+ and CD8+CD45RO+ T lymphocyte proliferation was elevated in HTLV-1-infected subjects compared with controls, with an extra 1012 lymphocytes produced per year in an HTLV-1-infected subject. The in vivo proliferation rate of CD4+CD45RO+ cells also correlated with ex vivo viral expression. Finally, the inflammatory disease HTLV-1-associated myelopathy/tropical spastic paraparesis was associated with significantly increased CD4+CD45RO+ cell proliferation. We suggest that there is persistent viral gene expression in vivo, which is necessary for the maintenance of the proviral load and determines HTLV-1-associated myelopathy/tropical spastic paraparesis risk. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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5. Quantification of the virus-host interaction in human T lymphotropic virus I infection.
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Asquith, Becca, Mosley, Angelina J., Heaps, Adrian, Tanaka, Yuetsu, Taylor, Graham P., McLean, Angela R., and Bangham, Charles R. M.
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ETIOLOGY of diseases , *VIRUS diseases , *IMMUNOLOGY , *HTLV-I , *HTLV , *LYMPHOCYTES - Abstract
Background: HTLV-I causes the disabling inflammatory disease HAM/TSP: there is no vaccine, no satisfactory treatment and no means of assessing the risk of disease or prognosis in infected people. Like many immunopathological diseases with a viral etiology the outcome of infection is thought to depend on the virus-host immunology interaction. However the dynamic virus-host interaction is complex and current models of HAM/TSP pathogenesis are conflicting. The CD8+ cell response is thought to be a determinant of both HTLV-I proviral load and disease status but its effects can obscure other factors. Results: We show here that in the absence of CD8+ cells, CD4+ lymphocytes from HAM/TSP patients expressed HTLV-I protein significantly more readily than lymphocytes from asymptomatic carriers of similar proviral load (P = 0.017). A high rate of viral protein expression was significantly associated with a large increase in the prevalence of HAM/TSP (P = 0.031, 89% of cases correctly classified). Additionally, a high rate of Tax expression and a low CD8+ cell efficiency were independently significantly associated with a high proviral load (P = 0.005, P = 0.003 respectively). Conclusion: These results disentangle the complex relationship between immune surveillance, proviral load, inflammatory disease and viral protein expression and indicate that increased protein expression may play an important role in HAM/TSP pathogenesis. This has important implications for therapy since it suggests that interventions should aim to reduce Tax expression rather than proviral load per se. [ABSTRACT FROM AUTHOR]
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- 2005
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6. The dynamics of T-cell fratricide: application of a robust approach to mathematical modelling in immunology
- Author
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Asquith, Becca and Bangham, Charles R.M.
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T cells , *IMMUNOLOGY - Abstract
Fratricide between
CD8+ T lymphocytes is known to occur in HTLV-I and possibly HSV-1 and HIV-1 infection. However it is not known what effect, if any, T-cell fratricide has on the course of infection. Here we present simple mathematical techniques to investigate T-cell fratricide with particular reference to HTLV-I infection. Using a general model we predict the qualitative and quantitative effect of fratricide on HTLV-I equilibrium proviral load. We also investigate the effect of fratricide on the probability of viral clearance.We show that, surprisingly, fratricide can lead either to an increase or a decrease in equilibrium proviral load. We derive the conditions necessary for fratricide to cause a decrease in load and deduce that, for the five HTLV-I-positive patients considered here, fratricide has probably caused an increase in equilibrium load. We also estimate the percentage increase in load that is attributable to fratricide and determine the parameters that should be measured in order to improve this estimate. Finally, we show that fratricide reduces the probability of viral clearance.Mathematical modelling of HTLV-I infection, as is often the case in biology, is severely hampered by a lack of experimental data. Consequently it is difficult to know what functional form a model should take. The behaviour of complex nonlinear systems is highly model-dependent. Predictions based on theoretical models are therefore sensitive to the choice of model; this is a very severe problem that undermines and limits the success of the application of mathematics to immunology. In this paper we reduce the model dependency of the results in two ways—by considering (analytically) a general model with a minimal number of assumptions and, where this is not possible, by checking (numerically) that a wide range of models yield the same results. We therefore begin to develop two practical methods for dealing with the problem of robustness in mathematical models of the immune system. [Copyright &y& Elsevier]- Published
- 2003
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7. Clonality, latency and integration of HTLV-1 in vivo.
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Bangham, Charles, Cook, Lucy, Laydon, Daniel, Asquith, Becca, and Melamed, Anat
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HTLV-I ,VIRAL genetics - Abstract
An abstract of the article "Clonality, latency and integration of HTLV-1 in vivo," by Charles Bangham and colleagues is presented.
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- 2013
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8. In contrast to HIV, KIR3DS1 does not influence outcome in HTLV-1 retroviral infection
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O’Connor, Geraldine M., Seich Al Basatena, Nafisa-Katrin, Olavarria, Viviana, MacNamara, Aidan, Vine, Alison, Ying, Qi, Hisada, Michie, Galvão-Castro, Bernardo, Asquith, Becca, and McVicar, Daniel W.
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HIV , *HEALTH outcome assessment , *HTLV-I , *ADULT T-cell leukemia , *PARAPARESIS , *RETROVIRUSES - Abstract
Abstract: While most carriers of human T-cell leukemia virus type 1 (HTLV-1) remain asymptomatic throughout their lifetime, infection is associated with the development of adult T-cell leukemia (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). The exact parameters that determine these outcomes are unknown but are believed to include host genetic factors that control the immune response to infection. Host response to fellow retroviridae member HIV is influenced by the expression of members of the Killer Immunoglobulin Receptor (KIR) family including KIR3DS1. In this study we examined the association of KIR3DS1 with the outcome of HTLV-1 infection in three geographically distinct cohorts (Jamaican, Japanese and Brazilian). Despite increased prevalence of KIR3DS1 in the HAM/TSP patients of the Jamaican cohort, we found no evidence for a role of KIR3DS1 in influencing control of proviral load or disease outcome. This suggests that unlike HIV, KIR3DS1-mediated regulation of HTLV-1 infection does not occur, or is ineffective. [Copyright &y& Elsevier]
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- 2012
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