Search

Grapevine trunk diseases (GTDs) are a major threat to the wine industry, causing yield loss and dieback of grapevines. While the increasing damage caused by GTDs in recent decades have spurred several studies on grapevine-associated pathogenic fungi, key questions about the emergence and severity of GTDs remain unanswered, including possible differences in plant pathogenic fungal communities in asymptomatic and symptomatic grapevines. We generated fungal DNA metabarcoding data from soil, bark, and perennial wood samples from asymptomatic and symptomatic grapevines sampled in three terroirs. We observed larger compositional differences in plant pathogenic fungi among different plants parts within grapevine plants than among individual grapevines. This is driven by the dominance of GTD-associated fungi in perennial wood and non-GTD pathogens in soil, as well as by the lack of significant differences among asymptomatic and Esca symptomatic grapevines. These results suggest that fungi generally associated with Esca disease belong to the core grapevine microbiome and likely are commensal endophytes and/or latent saprotrophs, some of which can act as opportunistic pathogens on stressed plants. In addition, we found significant compositional differences among sampling sites, particularly in soil, which suggest a certain influence of local edaphic and mesoclimatic factors on plant pathogenic fungal communities. Furthermore, the observed differences among terroirs in plant pathogenic fungal communities in grapevine woody parts indicate that environmental factors likely are important for the development of Esca disease and further studies are needed to investigate the abiotic conditions on fungal compositional dynamics in Esca-affected plants.

Hard cider is an important and growing part of the U.S. beverage market. Previous research suggests there is an opportunity for growers interested in selling locally grown cider-specific apple (Malus domestica) varieties. However, cider apple growers face production, distribution, and marketing challenges. This article fills a gap in the literature using survey data from four states. We find that growers are interested in expanding cider apple production to supply local craft cider makers, but may be constrained by gaps in current production information, such as how to grow cider varieties. Uncertainty about the regional suitability of different varieties, disease management, and the willingness of cider makers to pay a premium for cider apple production constitute significant concerns. Survey respondents most commonly requested information on horticultural qualities of varieties and disease management. Top marketing needs include the ability to garner premium prices. A regional “terroir” approach to cider marketing holds promise.

Winter hardiness may be influenced by vineyard terroir-driven factors, and vineyard zones with low water status [leaf water potential (ψ)] could be more winter hardy than vines with high water status (higher leaf ψ). Six Cabernet franc vineyards were chosen throughout the Niagara region in Ontario. Data were collected at fruit set, lag phase, and veraison [soil water content (SWC), leaf ψ], at harvest (yield components, berry composition), and three times during winter (LT50; the temperature at which 50% of buds die) in the 2010–2012 seasons. Interpolation by kriging and mapping of variables was completed using ArcGIS, and statistical analyses (linear correlation, k-means clustering, principal components analysis, multilinear regression) were performed. Spatial trends were observed in each vineyard for SWC, leaf ψ, yield components, berry composition, and LT50. Geographic information systems (GIS) and statistical analysis revealed that leaf ψ could predict LT50, with strong positive correlations between LT50and leaf ψ values in most vineyards in 2010–2011. In the dry 2012 season, leaf ψ (particularly at veraison; range −1.3 to −1.6 MPa) was positively correlated to LT50, yield, titratable acidity (TA), pH, and Brix and negatively to SWC, color, anthocyanins, and phenols. Overall, vineyards in different appellations (Niagara Lakeshore, Lincoln Lakeshore, Four Mile Creek, Beamsville Bench) showed many similarities. These results suggest that there is a spatial component to winter injury, as with other aspects of terroir. Furthermore, this study allows for means by which to compare winter hardiness to other critical variables to better understand the terroir of the Niagara region.

The influence of growing region on hop quality was examined in three American aroma hop varieties (Mosaic®, Simcoe® and Strata®) that were harvested from two to five distinct sites within each of eleven commercial fields located throughout the Willamette Valley, Oregon. Weather, climate, and management data were collected for each of the eleven fields, while site-specific soil characterization was performed at each of the 50 sites. While hop variety had the largest influence on chemical differences, Multiple Factor Analysis revealed regional groupings attributable to differences in soil, weather, and management. Supplemental data for this article is available online at at https://doi.org/10.1080/03610470.2021.1968272 .

The influence of growing region on hop quality is examined in three American aroma hop varieties (Mosaic®, Simcoe® and Strata®) from eleven locations throughout the Willamette Valley, OR. Hops from each site were analyzed for total oil content, α- and β-acids, and the oil was analyzed via GC-FID to quantify selected aroma compounds. Single-field IPAs were brewed using composite samples from each field and assessed via sensory evaluation. While hop variety had the largest influence on chemical differences, as expected, Analysis of Variance revealed differences between fields within the same variety. Sensory discrimination and descriptive testing of the single-field IPAs also showed significant region-based differences among samples within the same variety. Supplemental data for this article is available online at

The abstract is available here: https://uscholar.univie.ac.at/o:1671457

Wine grape berries (Vitis spp.) harbor a wide variety of yeasts and filamentous fungi that impact grapevine health and the winemaking process. Identification of these fungi could be important for controlling and improving wine production. The use of high-throughput sequencing (HTS) strategies has enabled identification and quantification of bacterial and fungal species in vineyards. The aims of this study were to identify mycobiota from Cabernet Sauvignon and Zinfandel (V. vinifera), Carlos and Noble muscadines (V. rotundifolia), Cynthiana (V. aestivalis), and Vignoles hybrid (cross of different Vitis spp.) grapes, and investigate the effect of grape variety, location, and year on grape fungal communities. Grape berries were collected in 2016 and 2017 from four vineyards located in Arkansas. The HTS of the Internal Transcribed Spacer 1 region was used to identify grape indigenous epiphytic and endophytic fungal communities. The predominant genera identified on the Arkansas wine grapes were Uwebraunia, Zymoseptoria, Papiliotrema, Meyerozyma, Filobasidium, and Curvibasidium. Overall, the data suggested that grape fungal community distribution and relative abundance were influenced by grape variety, year, and location, but each was influenced to a different extent. Not only were grape mycobiota influenced by year, variety, and location but also it appeared that communities from the previous year impacted microbial communities the following year. For example, an increase of the mycoparasite Ampelomyces quisqualis was noticed in 2017 on grapes that carried the causal agent of powdery mildew, Erysiphe necator, in 2016, thus, amplifying the importance of vineyard microbiota knowledge for disease management and winemaking.

‘Microbial terroir’ relates to the influence of autochthonous yeasts associated with a grape cultivar on the resultant wine. Geographic region, vineyard site and topography, climate and vintage influence the biodiversity of these microbial communities. Current research focus attempts to correlate their ‘microbial fingerprint’ to the sensorial and chemical characteristics of varietal wines from distinct geographical wine regions. This study focuses on the minor red grape variety, Negro Saurí, which has seen a resurgence in the León Appellation of Origin in Spain as a varietal wine. An experimental vineyard at Melgarajo S.A. (42° 15′ 48.68_N 5° 9′ 56.66_W) was sampled over four consecutive vintages, with autochthonous yeasts being isolated from grapes, must and pilot-scale un-inoculated fermentations, and identified by ITS sequencing. Forty-nine isolates belonging toMetschnikowia pulcherrima,Lachancea thermotolerans,Hanseniaspora uvarumandTorulaspora delbrueckiiwere isolated from grapes and must, and early stages of fermentation dependent on seasonal variation.Saccharomyces cerevisiaepredominated throughout fermentation, as a heterogeneous and dynamic population, with seven major biotypes identified amongst 110 isolates across four consecutive vintages. Twenty-fourS. cerevisiaeisolates representing five strains dominated in two or more vintages. Their persistence through fermentation warrants further validation of their oenological properties as starter cultures.

Aim: The aims of this study were to (1) formulate a baseline understanding of the performance of the indigenous Cypriot white grape Xynisteri and the red grape Maratheftiko (Vitis vinifera L.), and (2) compare these varieties to Shiraz and Sauvignon blanc grown in a Cypriot vineyard.Materials and results: The investigation involved multiple dry grown vineyards from the Krasochoria region of Lemesos, Cyprus, during the 2017, 2018 and 2019 vintages. Vine performance measurements, including midday stem water potential, stomatal conductance, chlorophyll content, stomata density, vine phenology and vegetative and reproductive measurements, were taken at flowering, veraison and pre-harvest. Xynisteri had the greatest stomatal density, more shoots, more leaves, heavier bunches, greater yield, higher leaf water potential at harvest, and a stomatal conductance equal to Maratheftiko, but greater than that of both Shiraz and Sauvignon blanc. Maratheftiko had the longest shoots, largest shoot diameter and the greatest chlorophyll content out of all four varieties.Conclusions: This study identified the ability of the indigenous Cypriot grape varieties, Xynisteri and Maratheftiko, to better tolerate hot and dry conditions when compared to more commonly cultivated varieties grown in the same environmental conditions.Significance and impact of the study: The changing climate of wine growing regions worldwide is placing great pressure on the resources for sustainable viticulture. Many vineyards in hot climate zones base their businesses on European grape varieties traditionally grown in regions with abundant water resources. It is therefore necessary for the global wine industry to investigate grape varieties that are indigenous to hot climates. The eastern Mediterranean island of Cyprus is one such place, with more than 10 indigenous grape varieties that grow well in a hot climate without irrigation. Consumer studies have demonstrated that wines made from these Cypriot varieties are equally, if not more, acceptable than wines made from more traditional European grapes; therefore, the potential for their use in other hot wine growing regions is promising.

Traditional ‘terroir zoning’ has largely relied on heuristic ‘expert’ opinion coupled with approaches to land classification based on thematic mapping to describe the influence of soil conditions and climate on wine composition. Recent advances in geographical information systems (GIS) and digital mapping have enabled more robust quantitative methods to be developed, but with few exceptions recent terroir research has remained reliant on heuristic opinion and conformity to previously defined terroir units, rather than employing data-driven approaches. Using two case studies at regional scale, the aim of this paper is to illustrate how the use of methods of quantitative spatial analysis, as used to guide understanding of production system variability and to underpin precision viticulture (PV), may assist in better understanding terroir at a range of scales. In the Barossa region of Australia, cluster analysis of indices of soil physical and chemical fertility (available water capacity and cation exchange capacity), with critical climate variables (growing season rainfall, mean January temperature and growing degree days), clearly delineates differences between the Barossa and Eden Valleys but does not robustly promote further sub-division. Meanwhile, in the Marlborough region of New Zealand, interpolation of data supplied by wine companies from over 450 vineyards over several seasons suggests a consistent and characteristic regional ‘terroir’ in terms of vine yield and harvest date. Similarly consistent results were obtained for sub-regions of the Wairau Valley and a comparison of the Wairau and Awatere valleys. Thus, with scale-dependent modification, the methods of spatial analysis used to underpin PV and studies of within-vineyard variability offer much potential for terroir analysis and the identification of terroir zones. Importantly, these methods are unbiased, data-driven, and not reliant on heuristic opinion.

Understanding how different pedoclimatic conditions interact with vine and berry physiology, and subsequently impact wine quality, is paramount for an good valorization of viticultural terroirs and can help to optimize mitigation strategies in the face of global warming.The aim of the present study was to establish terroir zones in a steep slope region in Switzerland based on vine and berry physiology. The study area, Villette in the AOC Lavaux, was a unique experimental site due to the homogeneity of plant material in a relatively small microclimate (140 ha) and a multiplicity of different expositions, soil types and altitudes. Vine and berry physiology as well as temperature of twenty-two plots were monitored during three consecutive seasons to investigate whether a link with pedoclimatic parameters can be established.The annual temporal variation of the average temperature was 142 growing degree days (GDD) over all years. Remarkably, spatial temperature variability was twice as high, with a variation between most extreme plots of 395 GDDs on average over all years. PCA and hierarchical clustering of assessed vine and berry physiological parameters resulted in a vintage dependent grouping of plots differing between years, which was not congruent with geological entities. This highlights the importance of the vintage effect, which had a large influence on vine and berry physiology and impacted terroir zones more than soil groups. Important differences in budburst and flowering were observed between plots, whereas altitude was the main driver of precocity in all years, being relatively independent of the vintage, which confirms the importance of topography in viticultural terroirs.

Background and Aims Wines that exhibit regional characters are often the most sought after and the highest valued wines available in the marketplace. This study evaluated the sensory properties that were regionally distinctive for Australian Shiraz wines. Methods and Results Sets of wines (22–28 wines) from six prominent Australian Shiraz‐producing regions were initially evaluated by groups of local winemakers using a rapid sensory method called Pivot Profile (PP) to obtain maps of their sensory characteristics. Three or four wines from each region were then selected using cluster analysis of the PP data and were evaluated using sensory descriptive analysis. The regional PP assessments provided a sensory fingerprint of the variability of each of the regions studied and identified sensory characteristics that typified the largest groups of wines of each region. The descriptive analysis highlighted sensory characteristics that distinguished the wines from the different regions, including mint, cooked vegetal, viscosity, dark fruit and savoury attributes. Conclusions This work has provided detailed quantitative data on the sensory properties associated with each of the regions and demonstrated that there are distinctive, region‐specific sensory characteristics. Significance of the Study Sensory fingerprints that differentiate one region from another will aid producers and the trade in appreciating what can be expected from different regions; they allow targeting of production decisions to enhance distinctive sensory attributes and will assist in improved communication between marketers and consumers.

Grapevine winter hardiness is governed by several factors besides extreme weather conditions, such as site-specific soil factors (texture, composition, moisture, drainage), vine water status, and yield. It was hypothesized that winter hardiness would be influenced by specific vineyard terroir-driven factors and that zones in vineyards with low water status (leaf water potential [Ψ]) would likewise be more winter hardy than vines with high water status (less negative leaf Ψ). Six Riesling vineyards were chosen throughout the Niagara region in Ontario. Data were collected at fruit set, lag phase, and veraison (soil water content [SWC], leaf Ψ), at harvest (yield components, berry composition), and three times during the winter (LT50; the temperature at which 50% of buds die) in 2010 to 2012. Interpolation and mapping of variables was completed using kriging, and statistical analyses (linear correlation, k-means clustering, principal components analysis, multilinear regression) were performed. SWC, leaf Ψ, yield components, berry composition, and LT50 were spatially clustered in each vineyard. GIS and multilinear regression revealed that leaf Ψ could predict the LT50 value, with strong correlations observed between LT50 and leaf Ψ values in most of the vineyard blocks in 2010 to 2011 (4/6 and 5/6, respectively). In the extremely dry 2012 season, leaf Ψ (range across sites at veraison 0.9 to 1.4 MPa) was positively correlated to LT50, yield, titratable acidity, pH, and Brix and inversely correlated to SWC and monoterpene concentration. Results suggest that there is a spatial component to winter hardiness, as with other aspects of terroir. This study allows a method to compare winter hardiness to other critical variables to better understand terroir-based factors of the Niagara region.

The article investigates how wineries have used different markers as an effort to differentiate themselves from other producers and support their self-identity. The most dominant markers perceived by the respondents are tied to different forms of regional/geographical markers, such as terroir including the AOC, and organic production. They stress the vine and agricultural aspects of winemaking. In addition, some markers also include special wine, such as old vine as a special aspect of identity as quality producers. The empirical basis is 23 face-to-face in-depth qualitative semi-structured interviews with owners and winemakers in wineries in New South Wales, Australia, Provence, France and by the river Duero, Spain; and two wine experts in Australia and in Spain. The analytical perspective includes the notion of boundary objects, and the role of technological actors in an Actor-Network theoretical approach. We also make use of the framework of small business identity as production oriented. The main conclusion of the paper is that wine producers in the market of quality wine tie their efforts to differentiate their quality wine to their identity work.

Grapevines are being challenged by climate changes, forcing winemakers to implement irrigation systems to cope with excessive water stress. Previous studies focused on a small set of international varieties, and only few data are available for terroirs hosting cultivars with possibly different responses to drought stress. In this light, we monitored grapevine water status and grape’s physical and chemical composition, as well as concentration and structural characteristics of grape extractable polyphenols, in ten different Refosk vineyards located in the Classical Karst terroir during 2018 and 2019. Grapevines did not suffer severe stress during the two years, but their response to water shortage periods was highly heterogeneous, as pre-dawn (Ψpd) and minimum (Ψmin) leaf water potential significantly differed between vineyards, especially during the drier part of the season. Moreover, the timing of maximum water stress differed in the two years, as in 2019 longer water shortage periods and higher temperature occurred at flowering stage and before veraison, while in 2018 they were higher after veraison. These differences influenced berry’s quality, as titratable and malic acid concentration in juice, as well as total anthocyanin, total polyphenols and higher high molecular weight proanthocyanidins (HMWP) concentration in skins, were higher in 2019 than in 2018. Regarding seed proanthocyanidins, HMWP concentration, mean degree of polymerisation (mDP) and percentage of galloylation (G) in seeds were higher in 2018 than in 2019. The differences in water status measured in spatially close-related vineyards strongly support the importance of monitoring grapevines’ water status dynamics to design adequate and effective water management activities rather than relying on climate data solely. Moreover, the timing of water shortage periods also played a role in determining Refosk grape quality. Our analyses showed that the higher (but still moderate, with Ψpd and Ψmin mean values around −0.50 and −1.25 MPa, respectively) water stress between veraison and harvest occurred in 2018 might reduce Refosk grape acidity and increase concentration, polymerisation and galloylation of seed extractable proanthocyanidins.

Tokaj is an important Central European wine-growing area with controlled planting and authorized varieties of white vines. This area has a specific microflora composition which changes based on its climate dependence, as well as during the fermentation process of wine production. Therefore, the aim of this study was, by culture examination of the samples, to detect the microbiota of soil, leaves, berries and fermentation must from two vineyards from the Slovak part of Tokaj. The highest total viable count (5.60 ±0.01 log cfu.g-1) and the highest total yeast and mould count (4.32 ±0.01 log cfu.g-1) in soil samples were recorded in vineyard Berecký. The highest total viable count in soil samples (6.71 ±0.01 log cfu.g-1) was confirmed by examination of samples originating from the vineyard of Čierna Hora. When determining the total yeast and mould count, the highest numbers were recorded in the must samples (4.15 ±0.01 log cfu.mL-1). Lactic acid bacteria were collected in samples from both vineyards, only in very low numbers. Overall, statistically significant differences (p

Grapevine water status of Vitis vinifera cvs. Riesling, Cabernet franc, and Pinot noir was evaluated in Ontario, Canada, using leaf water potential (Ψ) and surface soil water content (SSWC; %) to examine spatial relationships between soil and vine water status and berry composition variables, particularly secondary metabolites. The goal was to assess their significance as drivers of the terroir effect. Research was conducted over two vintages (2014 and 2015). Strong inverse relationships were found between vine water status and total phenols in Cabernet franc and Pinot noir and monoterpenes in Riesling. Leaf Ψ was associated with berry size, while yield had strong negative correlations with pH and (in most cases) positive correlations with vine size. Principal component analysis complemented with k-means clustering identified relationships between variables, while Moran’s index also indicated strong clustering patterns for SSWC. Both SSWC and leaf Ψ showed weak correlations with each other. Overall, leaf Ψ was a stronger indicator of important secondary metabolites (anthocyanins, phenols, color, and monoterpenes) than SSWC.

International audience; Microorganisms associated with plants are determinant for their fitness, but also in the case of vine grapes, for the quality and quantity of the wine. Plant microbiota is, however highly variable in space despite deterministic recruitment from the soil reservoir. Therefore, understanding the drivers that shape this microbiota is a key issue. Most studies that have analysed microorganisms associated with vines have been conducted at large scales (e.g., over 100 km) and have analysed the bulk soil and the rhizosphere. In this study, we focused on the root-microbiota endosphere, the most intimate fraction of microorganisms associated with plants. We sampled vine roots in 37 fields distributed throughout a vineyard to investigate drivers shaping the grapevine microbiota at the alpha-(i.e., within-field) and gamma-(i.e., between-field) diversity scales. We demonstrated that vine endospheric microbiota differed according to both the edaphic and plant-specific parameters including cultivar type and age. This work supports the idea of an existing microbial terroir occurring within a domain and offers a new perspective for winemakers to include the microbial terroir in their management practices.

International audience; Grapevine decline is a top concern in viticulture worldwide and is often associated with many biotic and abiotic factors. Grape trunk diseases and viruses are some of the most frequently identified causes of vine dieback. However, a decline is sometimes observed when no mineral deficiency or excess, or pathogenic causes can be identified. Soil enzymatic and microbial activities are relevant bio-indicators since they are known to influence vine health. Grapevine associated microbiota, linked to vine fitness, is known to be influenced by soil microbiota coming from the microbial pool inhabiting the vineyard. This work describes the microbial diversity and activity of four different vineyard plots of the Bordeaux region, selected due to the presence of localised declining areas unexplained yet by disease symptoms. Soils were sampled in declining areas and areas within the same plot showing no decline symptoms, during autumn and spring periods. Significant differences in enzymatic activities, microbial biomass and activity were found among soils even if those soils presented quite similar physicochemical characteristics that could not explain these observed declines. The results of enzymatic assays distinguished patterns in autumn and spring periods with an overall greater enzymatic activity in soils from non-declining areas. This work suggests that soils displaying decline symptoms present a dysbiosis in functionality and diversity which is linked to vine health.

Modern almond growing travels on the tracks of super-high density (SHD). Born in 2010, it has already reached 6700 ha planted all over the world. This new cultivation system needs to define efficient agronomic techniques in order to identify it as a “Super-Efficient System”. Among these, the choice of cultivar is a crucial technique and a key factor for sustainability. The purpose of this study was to compare different cultivars in terms of vegetative, productive, and efficiencies parameters in order to gain applicable relevant knowledge about the SHD almond cultivation technique. For this, 3 years of research was carried out during 2017–2019, on a young almond grove made in 2014 with row spacing of 3.80 m × 1.20 m (2190 trees/ha), to evaluate the agronomic behavior of the two most planted cultivars in Italy, Guara-Tuono and Lauranne® Avijor, grafted on the Rootpac®20 dwarfing rootstock. The main biometric, productive, yield, mechanical harvesting efficiencies, and almond quality parameters were evaluated. Cv Lauranne® showed greater vigor, greater fruit yield, and damaged axes by mechanical harvesting, while higher values of yield efficiencies were observed for cv Tuono. Harvesting efficiency was related to canopy size and tree age. On the contrary, almonds quality parameters were strongly related to the cultivar, confirming the good performance of Tuono as varietal characters. Then, this cultivar seems to be the most suitable for an efficient SHD planting system, in line with the objectives of modern sustainable fruit growing. The better performance of cv Tuono could be related to the positive influence of the terroir as well.

The chemical and sensory characteristics of the wines are related to the geographical origin of the grape, as a result of the interplay between the plant material (G), its acclimatization to the environment (E) and the human factor that influences both the vineyard and the winery. The range of phenotypes that a single genotype can express depending on its environment is known as phenotypic plasticity and is the result of G × E interaction. The present study evaluated the independent and interactive effects of Cabernet Sauvignon plant materials (G: Clone 7 and Mount Eden) implanted in different geographical indications of Mendoza, Argentina (E: Agrelo, Pampa El Cepillo, Altamira and Gualtallary) according to fruit yield and phenolic profiles of wines. The experiment was carried out during 2018 and 2019 vintages using a multifactorial design. When berries reached 24 °Brix, the clusters were harvested, analyzed and wines elaborated by a standardized procedure. Then, the anthocyanin and non-anthocyanin phenolic profiles of wines were determined by high-performance liquid chromatography with diode array and fluorescence detection (HPLC-DAD–FLD). The results revealed significant G × E interactions for yield traits, including the number of clusters per plant. Differential chemical composition and quality parameters of the resulting wines, markedly affected by E, were observed; that is the geographical location of the vineyards. There were similarities in the phenolic composition between Pampa El Cepillo and Altamira, while larger differences between Agrelo and Gualtallary were observed. Gualtallary presented the highest levels of anthocyanins, quercetin and trans-resveratrol. The increased amount of these compounds in Gualtallary was associated with an increased UV-B exposure of plants at this high altitude environment. This is the first report that characterizes the effects of plant material and environment for Cabernet Sauvignon. These results are of oenological and viticulture interest for the wine industry demonstrating that the selection of the plant material and the vineyard location for Cabernet Sauvignon can considerably affect the quality attributes of wines. Fil: Muñoz, Flavio Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Urvieta, Roy Alexander. Catena Institute Of Wine.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Buscema, Fernando. Catena Institute Of Wine.; Argentina Fil: Rasse, Manuel. School of Agriculture Ingenieers; Francia Fil: Fontana, Ariel Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Berli, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina

Worldwide, there are thousands ofVitis viniferagrape cultivars used for wine production, creating a large morphological, anatomical, physiological and molecular diversity that needs to be further characterised and explored, with a focus on their capacity to withstand biotic and abiotic stresses. This knowledge can then be used to select better adapted genotypes in order to help face the challenges of the expected climate changes in the near future. It will also assist grape growers in choosing the most suitable cultivar(s) for each terroir; with adaptation to drought and heat stresses being a fundamental characteristic. The leaf blade of grapevines is the most exposed organ to abiotic stresses, therefore its study regarding the tolerance to water and heat stress is becoming particularly important, mainly in Mediterranean viticulture. This review focuses on grapevine leaf morphoanatomy - leaf blade form, leaf epidermis characteristics (cuticle, indumentum, pavement cells and stomata) and anatomy of mesophyll - and their adaptation to abiotic stresses.V. viniferaxylem architecture and its adaptation capacity when the grapevine is subjected to water stress is also highlighted since grapevines have been observed to exhibit a large variability in responses to water availability. The hydraulic properties of the petiole, shoot and trunk are also reviewed. Summarising, this paper reviews recent advances related to the adaptation of grapevine leaf morphoanatomical features and hydraulic architecture to abiotic stresses, mainly water and heat stress, induced primarily by an ever-changing global climate.

Studies on parentage and kinship of traditional Vitis vinifera grape cultivars allow light to be shed on the likely natural breeding at the basis of the assortment evolution of cultivated grapevines in a particular area over several centuries. They therefore contribute to our understanding of the process of domestication and local development of viticulture through farmer selection and allow us to infer the origin and history of traditional grape cultivars. For wine grapes, this evidence adds significant marketing value to wines and their terroirs, being highly appealing for wine consumers and wine lovers. Around 200 grape cultivars, traditional to north and north-western Italy, and nearly 700 unique cultivars mainly from other Italian regions and Central Europe were examined for genetic kinship through nuclear microsatellite markers (nuclear simple sequence repeats; nSSRs). More than 100 genotypes showed parent/offspring or kinship relationships, as revealed by 32 SSR loci. Forty trios of parents and descendants were disclosed, among which was the pedigree of 'Dolcetto', the third most widely planted cultivar in Piedmont. For samples also analysed by the 20K Illumina SNPs chip, the parental relationships were confirmed. The results, corroborating a pattern shown by other studies, indicate closer kinship among cultivars from the same geographical area, and a complex network of pedigrees with a few major founders showing 12-21 links with other cultivars. These main genitors are often cultivars that are no longer cultivated and/or are nowadays threatened with extinction. On one hand, this highlights the urgency of rescuing and preserving these key genetic links before they are lost. On the other hand, it explains the still unsolved jigsaw puzzle of the origin of many renowned grape cultivars. Also, their ampelographic features provide significant clues for linking their identity with ancient cultivars depicted or described centuries ago, shedding light on the history of descendant cultivars of current high economic value.

Volatile composition plays a fundamental role in wine aroma and quality. However, the concentrations of volatiles depend on climate conditions and agricultural practices, such as irrigation. The aim of the current study was to assess the effects of irrigation on the volatile composition and sensory characteristics of wines from Vitis vinifera (L.) cultivar ‘Albarino’ in two zones of NW Spain (Rias Baixas and Ribeiro) during three consecutive years (2012, 2013, and 2014). Irrigation was scheduled by the vine grower in Rias Baixas, whereas it was fixed at 50% of the estimated crop evapotranspiration in Ribeiro. Water was applied in August, mainly, in Rias Baixas and from mid-July to mid-August in Ribeiro. Irrigation significantly altered the concentrations of several volatiles, which differed between zones and years. For instance, wines from the irrigated treatment had lower concentrations of isoamyl acetate, 2-phenylethyl acetate, and ethyl hexanoate than those from the rain-fed treatment in Rias Baixas. In contrast, irrigation increased the concentrations of ethyl hexanoate and ethyl octanoate, while reduced those of free terpenes in wines from Ribeiro. Mainly, these differences between zones were caused by the climate conditions of each region.

The concept of ‘terroir’ has roots steeped in French history of wine and evocative stories of the land. Today terroir is important as a quality marker in brand management for upmarket wines. French wines have benefited from their historical connotation with terroir but other wine growing countries have also adopted the term to mean characteristics in wine attributable to place of origin. However there is no universally accepted definition of terroir except to say it refers to a particular place. This paper addresses this ambiguity by seeking the practice-based views of three experienced non-French winemakers. This reveals a social dimension to terroir that in various degrees supports and extends both the technical/scientific progress and brand value for premium wines in both Old and New World regions. This article contributes to the growing body of research that seeks to understand the practicality of terroir as a marketing and brand value indicator.

The terroir concept can be defined as the effect on food sensory features of the uniqueness of the place, which gives food products a particular taste, mainly due to soil and climate characteristics. The aim of the present study was to understand the relationship between the physiological behaviour of vineyards, soil characteristics and final quality of ‘Crimson’ table grapes. Soluble Solids Content (SSC) and texture properties of berries varied significantly between the two vineyards. Unexpectedly, the vineyard which had the lower leaf chlorophyll content (9.99 mg m−2 ± 2.73), produced the heavier berries (5.82 g ± 0.21) with higher SSC values (22.03°Brix ± 0.23). Only slight differences for phenolic compounds and antioxidant capacity were observed between grapes from the two vineyards (p > 0.05). As previously reported by other authors, the balance between soil and berry mineral content seems to indicate that mineral nutrient uptake by the vine or soil ability to provide nutrients, did not step in significantly on grape quality. Regarding sensory evaluation, consumers found differences between grapes from the same cultivar growing in the same region but from two distinct vineyards (p

This study arose from the need to relate specific terroir aspects with experienced sensory properties of Gewürztraminer wines from Tramin (northern Italy). A multidisciplinary approach was used to investigate seven vineyards’ ecological characteristics, including geology and geographical features. A geopedological method using Vineyard Geological Identity (VGI) and Solar Radiation Identity (SRI) for topoclimatic classification, as well as multi-parameter measuring stations of air and soils, elicited analytical data for qualitative and quantitative terroir characterisations. Furthermore, wide-ranging and targeted oenological and chemical analyses were conducted on grapes, musts and wines to correlate their biochemical compositions with the measured terroir conditions. The study identified strong connections between vineyard geo-identity and wine mineral fingerprint, confirming mineral traceability of Rb/Sr ratio and of some minerals common to the local geology, such as Ba, Rb, Mn and Be. In particular, the most differing geo-mineral and physical soil conditions of two studied vineyards are apparent in the oenological components, flavours and aromas of their musts and finished wines. Amino acids, primary varietal aromas and polyphenols, thiol compounds with tropical scents, phenolic compounds with spicy notes and terpenic compounds, such as geraniol and citronellol, were related differently between fine-textured, more siliceous soils of glacial origin and coarser-textured, more dolomitic soils of local debris flow origin.

Hops (Humulus lupulus L.) is a species that grows spontaneously in Corsica, but the characterization of this species in this territory has not yet been investigated. The main objectives of this study are to explore the features of wild hops from Corsica and to determine the effect of the island terroir on some cultivars in the first year of growth. A multidisciplinary approach consisting of the genetic analysis, morphological comparison and chemical characterization of essential oils was carried out on four wild Corsican hops and three hop cultivars grown in Tettnang, Germany and Corsica, France. The morphological and GC-MS analysis of Corsican wild hops, set cluster coastal samples apart from the one far from the coast. This dissimilarity is supported by the SSR analysis by two of the three coastal accessions. The genetics demonstrate a proximity between the European noble cultivar Tettnanger and the mountain Corsican wild hop from Corte. The morphological comparison between German hops cultivated in Tettnang and in Corsican soil, and the GC-MS characterization of their essential oils’ chemical profiles, show different features between year 0 and year +1 for each sample. This multidisciplinary approach highlights an acclimatization of hop cultivars to the Corsican terroir one year after planting.

Climate is one of the components of terroir that most influences grape growth and development. Vines might suffer significant impacts under climate change, although they can be different depending on the variety and location. The aim of this study was to analyze vine phenology and grape composition variability related to weather conditions in La Mancha Designation of Origin, in Spain, and potential changes caused by climate change. The research focuses on the Tempranillo and Chardonnay varieties. Phenological dates and grape composition at ripening were evaluated for the period 2000–2019 and related to climatic variables. This information was used to project the potential variations under different emission scenarios (Representative concentration pathways RCP4.5 and RCP8.5) for 2050 and 2070. An advance in phenology is projected under climate change, for the two varieties, in agreement with that observed in years with different weather conditions. The advance for 2050 could be of 6, 11, 9, and 8 days for Tempranillo and 4, 9, 9, and 10 days for Chardonnay, respectively, for bud break, flowering, veraison, and maturity under the RCP4.5 scenario, and up to 50% higher under the PCP8.5 scenario. This advance in the phenological timing will imply ripening under warmer conditions, which could affect grape quality. A decrease in titratable acidity in both varieties is projected due to the increasing temperatures, which would have negative implications for the two varieties, and anthocyanin concentrations in Tempranillo are projected to suffer changes due to variations in temperature and in water deficits. The research presents a novelty for La Mancha DO, where there is no previous analysis in this respect. This is the first study in this vine-growing region in which projections are made on the potential changes for aforementioned vine cultivars, whose response is analyzed for expected warmer conditions in relation to those observed in cooler areas.

The aim of the study was to identify the ecological indicators that facilitate predictive analysis and to search for patterns in local geographical information to identify risks and opportunities in viticulture. The study focused on environmental factors that significantly affect the ripeness of the Gewürztraminer grape variety cultivated near Tramin, a village in northern Italy. In particular, the reliability of the new Solar Radiation Identity (SRI) topoclimate classification method was tested, along with its predictive capability in terms of the biosynthetic activity of the vine and the quality of its grapes. The SRI index characterises each vineyard in a precise and comparable way and helps to understand the way in which the topoclimate acts as an important abiotic stress factor for vines. A direct relationship between grape must sugar content and the SRI topoclimate index was observed. Our findings indicate an increase in sugar content of approximately 0.8 °KMW for every 10 points of the SRI index. Thus, a novel prediction model of grape ripening based on an SRI curve analysis is proposed. The correct application of the SRI index could be useful for discriminating and predicting geographical charactersitics of a given area strongly connected to ecological diversity and wine quality. It could support decision making in viticulture in terms of, for example, correctly matching vineyard and grape varieties, reducing wine vulnerability and production risk and predicting optimal ripeness and harvesting days. The use of the SRI prediction curve could help in adopting a more sustainable approach to agriculture and in finding new methods for adapting to climate change, such as by improving the match between the cultivars’ phenological status, vineyard location and growing season average temperature.

The aim of this work was to investigate the effect of meteorological conditions on resveratrol concentration of red wines produced in Piacenza viticultural region (Italy). In this regard, six representative estates producing Colli Piacentini Gutturnio DOC (a blend of V. vinifera L. cvs. Barbera and Croatina) vintage wines were analysed for trans- and cis-resveratrol over an 8-year period (1998&ndash, 2005). Grapes were taken from the same vineyard in each estate by using the same enological practices over the entire investigated period. The meteorological conditions corresponding to the production areas were recorded, and bioclimatic indices were calculated as well. Overall, cis-resveratrol concentration was negatively correlated to Huglin index and August mean temperature, whilst positive correlation coefficients were found when considering the Selianinov index and the rainfall of September.

The diversity of both bacterial and fungal communities associated with mango surface was explored using a metabarcoding approach targeting fungal ITS2 and bacterial 16S (V3-V4) genomic regions. Fruits were collected in Reunion Island from two different orchards according to a sampling method which allowed the effect of several pre-harvest factors such as geographical location (terroir), cultivars, fruit parts, tree position in the plot, fruit position on the tree (orientation and height), as well as the harvest date to be investigated. A total of 4,266,546 fungal and 2,049,919 bacterial reads were recovered then respectively assigned to 3,153 fungal and 24,087 to bacterial amplicon sequence variants (ASVs). Alpha and beta diversity, as well as differential abundance analyses revealed variations in both bacterial and fungal communities detected on mango surfaces depended upon the studied factor. Results indicated that Burkholderiaceae (58.8%), Enterobacteriaceae (5.2%), Pseudomonadaceae (4.8%), Sphingomonadaceae (4.1%), Beijerinckiaceae (3.5%), and Microbacteriaceae (3.1%) were the dominant bacterial families across all samples. The majority of fungal sequences were assigned to Mycosphaerellaceae (34.5%), Cladosporiaceae (23.21%), Aureobasidiaceae (13.09%), Pleosporaceae (6.92%), Trichosphaeriaceae (5.17%), and Microstromatales_fam_Incertae_sedis (4.67%). For each studied location, mango fruit from each cultivar shared a core microbiome, and fruits of the same cultivar harvested in two different locations shared about 80% fungal and bacterial family taxa. The various factors tested in this study affected bacterial and fungal taxa differently, suggesting that some taxa could act as geographical (terroir) markers and in some cases as cultivar fingerprints. The ranking of the factors investigated in the present study showed that in decreasing order of importance: the plot (terroir), cultivar, fruit parts, harvest date and the position of the fruits are respectively the most impacting factors of the microbial flora, when compared to the orientation and the fruit position (height) on the tree. Overall, these findings provided insights on both bacterial and fungal diversity associated with the mango surface, their patterns from intra-fruit scale to local scale and the potential parameters shaping the mango microbiota.

Three vigor zones, identified in a Barbera vineyard by remote sensing at full canopy, were carefully ground-truthed to determine, over 2 years, the relative weight of soil factors in affecting within-field variability, and to investigate vigor zone influence on dry matter (DM) and nutrient partitioning into different vine organs. Regardless of season, high vigor (HV) achieved stronger vine capacity as total vegetative growth and yield while resulting in markedly less ripened fruits than low vigor (LV) vines. PCA analysis carried out on ten different soil and vine variables clearly separated the three vigor levels and the correlation matrix highlighted that the factors mostly contributing to HV were soil depth, soil K and P concentration, total available water, clay fraction and Nleafconcentration. Conversely, sand fraction was the main marker for LV. When annual DM retrieved in clusters, canes, leaves, and shoot clippings was calculated for each vigor level and expressed as content (i.e. kg/ha) there was a general decreasing trend moving from HV to LV. However, when DM partitioned to each organ was given on a relative basis (i.e. percentage over total) results were similar across vigor levels. Similarly, when nutrients were given as content (e.g. kg or g/ha) out of 120 within-vigor combinations (12 nutrients, 2 seasons, 5 organs), 65 showed a significant difference between HV and LV. Conversely, with data expressed on a concentration basis (i.e. % DM) the number of significant differences between the vigor level means fell to 15. The study strengthens the causal link between soil properties and intra-vineyard spatial variability and clarifies that patterns of dry matter and nutrient partitioning to different vine organs are mildly affected by vine vigor when referred on a relative basis.

Terroir is about the link between wine and its origin. It has long been understood by sensory evaluation that the taste of wine from a given variety can be related to its origins. Specific organoleptic characteristics of wine are influenced by environmental factors such as soil and climate. By deconstructing the effect of measurable soil and climate parameters on grape and wine aroma compounds, the terroir effect on wine typicity can be better understood. Climate influences on vine development and grape ripening are mainly associated with temperature, radiation and rainfall, while soil influences are primarily associated with water availability and nitrogen supply. Significant advances have been made over recent years in understanding wine aromas and their molecular basis and influences of climate and soil on a wide range of molecules responsible for wine aroma expression. This article aims to review these recent research advances to obtain a more comprehensive understanding of how terroir influences wine typicity. The effect of terroir on wine quality and typicity is sometimes considered intangible and difficult to explain on a scientific basis. By combining agronomic, analytical and sensory approaches, however, this review shows that the terroir effect is mediated by measurable factors that can easily be monitored in the vineyard. Assessment of the results compiled by this review allows the suggestion that terroir expression at specific sites might be maximized by choosing appropriate plant material in relation to soil and climate, by acting on manageable parameters like vine water and nitrogen status, or by implementing canopy management to modify microclimate in the bunch zone.

Vine genetics, fruit maturity, region and vineyard are perceived as factors that strongly influence Pinot noir grape and wine composition. Our study aims to understand the relationship between grape (and ultimately wine) composition and the physical appearance and performance characteristics of a vine (i.e. vine ideotype). Our experimental approach controlled these variables by studying within-block differences in vine performance across multiple seasons and vineyards. Grapes were sourced at commercial harvest from 20 single vines from 12 vineyard sites in three Pinot noir growing regions (Central Otago, Martinborough and Marlborough) of New Zealand.Across three vintages yields ranged from 0.1 kg to 6.3 kg per metre, but there was no general relationship between yield and berry soluble solids. On a vine by vine basis normalised yields did not correlate among seasons. Berry extract colour measures were, on average, three-fold higher in 2019 than in 2018.Principal Component Analysis has indicated that vintage dominated berry composition effects that might otherwise be associated with yield per vine, region and vineyard. The extent of the variation in performance of the same vines between seasons largely excludes factors that are stable between seasons as primary causes. Changes in management of the same vine from year to year appeared the most likely contributors to variation. We have derived highly significant negative linear relationships between vine yield class and the frequency of vines that were within a benchmark specification established for icon vines, providing evidence of the quality risk associated with higher yield. The results also indicate that a proportion of vines meet the benchmark specification at higher yields. From results to date we can further our research confident in the knowledge that factors such as vine yield, region or vineyard are, in themselves, unlikely to be the principal drivers of major differences in Pinot noir grape and wine composition.

In Southeast Brazil, the change of grape harvest from wet summer to dry winter through double-pruning management has improved the quality of wines, currently denominated winter wines. In order to better understand the influences of soil, macroclimate, and vineyard management in winter wines, we investigated seven vineyards in the states of Minas Gerais (Três Corações – TC, Três Pontas – TP, Cordislândia – COR, São Sebastião do Paraíso – SSP and Andradas – AND) and São Paulo (Itobi – ITO and Espirito Santo do Pinhal – PIN) during three consecutive growing seasons. The vineyards are located in warm temperate zones and grouped in four soil types: Acrudox in TC, AND and SSP, Hapludox in TP; Hapludult in AND and PIN; Eutrudept in ITO. The high clay content (> 35 %) observed in all soil types, associated to low evapotranspiration demand, avoided the occurrence of severe water stress, as observed by the high values of leaf and stem water potential, stomatal conductance, photosynthesis, and transpiration. Differences in vigor were more related to vineyard management and did not affect grape composition. Among vineyards, parameters for berry quality from ITO, such as sugar and acidity, were more associated to high soil sand content and winter temperature. No significant differences were found in anthocyanins and total phenols of berries among vineyards, suggesting that the high thermal range and low precipitation during autumn-winter, historically observed in all municipalities, seemed to be the main factor for improvement of phenolic compounds.

Carbon stable isotope composition of berry must at harvest (δ13C) is an integrated assessment of plant water status during grape (Vitis vinifera L.) berry ripening. Measurement of δ13C of grape juice is proposed as an alternative to traditional measurements of water status to capture the spatial variability of physiological response at the vineyard scale, i.e., zoning. We performed samplings at four different locations in California, United States, with three different cultivars of table and wine grapes (Cabernet Sauvignon, Merlot, Crimson-Seedless). Leaf physiology (photosynthesis, AN, stomatal conductance, gs) and stem water potentials (Ψstem) were routinely measured. The δ13C was measured at harvest and strong relationships were found between Ψstem (R2 = 0.71), stomatal conductance (R2 = 0.71), net carbon assimilation (R2 = 0.59) and WUEi (R2 = 0.53). The role of leaf nitrogen on the signal was assessed by evaluating relationships between leaf nitrogen and WUEi (R2 = 0.54), Ci/Ca (R2 = 0.51), δ13C (R2 = 0.44), and Ψstem (R2 = 0.37). Although nitrogen can be among the environmental factors able to affect the δ13C signal, this difference is only observable when variability in N is very large, by pooling different vineyards/varieties, but not at the within-vineyard scale. The utility of δ13C was further tested and measured on grape berries sampled on an equidistant grid in a 3.5 ha vineyard where Ψstem was also measured throughout the field season and used to delineate management zones. Physiological measurements and grape composition were correlated to soil electrical resistivity and satellite-derived vegetation index. The two management zones obtained by δ13C or Ψstem were spatially similar at 67% and allowed to separate the harvest in two pools having statistically different grape composition (soluble solids, organic acids, and anthocyanin profiles). Zoning by δ13C performed as well as zoning by Ψstem to separate grape phenolic composition, e.g., for selective harvest. Our results provided evidence that δ13C of grape must is a reliable and repeatable assessor of plant water status and gas exchange in vineyard systems that are crucial for zoning vineyards, even when irrigated, and for ground-truthing sensor maps in precision viticulture.

Sangiovese is the most cultivated red grape variety in Italy where it is certified for the production of several Protected Designation of Origin (PDO) wines, and it is one of the most cultivated Italian red grape varieties in California. Despite the global distribution of this variety, there is a lack of international studies on Sangiovese grapes and wines. For this reason, the present study aimed to compare 20 commercial Sangiovese wines from 2017 harvest, 9 produced in Italy (Tuscany) and 11 in California, in order to evaluate the intrinsic and perceived quality. The eligibility, identity, and style properties (the intrinsic quality) of the wines were evaluated. A group of 11 Italian experts evaluated the perceived quality by rating the typicality of the wines. The experimental data showed that the intrinsic quality of Sangiovese wine samples was affected by the growing area, in particular, the wine resulted very different for the color indices and polyphenol composition. The above differences in intrinsic quality levels did not lead to a different evaluation of the perceived quality (typicality) by the wine experts. The results evidenced that Sangiovese variety is recognizable also if grown outside its original terroir, and fresh and fruity wines were considered more typical. This study expands our current knowledge of Sangiovese wines and the contribution of regional characteristics to the composition of wine.

Wine is a product of grape juice fermentation by yeast. Terroir is a term that encompasses all environmental factors and interactions at a specific geographical site, resulting in the development of regional-specific microbial strains and grape metabolites. In this study we determine the distribution of vineyard-associated wine yeast strains and characterize the flavonoid profile of Pinot Noir grapes among 3 sub-regions in the Okanagan Valley (OV), a major wine region in British Columbia, Canada. Pinot Noir grape samples were collected from 13 vineyards among 3 sub-regions of the OV, namely Kelowna (KE), Naramata-Penticton (NP) and Oliver-Osoyoos (OO), within a week prior to the winery harvesting date in 2016 and 2017. A total of 156 spontaneous Pinot Noir fermentations were conducted and vineyard-associated Saccharomyces strains were isolated from fermentations that reached two-thirds sugar depletion. Using microsatellite genotyping, we identified 103 Saccharomyces cerevisiae strains and 9 Saccharomyces uvarum strains. We also identified Saccharomyces paradoxus in one vineyard using ITS sequencing. We developed a microsatellite database of 160 commercial S. cerevisiae strains to determine the identity of the isolated strains and we include the database herein. Commercial strains were widely distributed across the three sub-regions. Forty-two of our 103 S. cerevisiae strains were equivalent or highly similar to commercial strains whereas the remaining 61 were considered as 'unknown' strains. Two S. uvarum strains were previously isolated in other OV studies and none matched the S. uvarum commercial strain BMV58. S. cerevisiae population structure was driven by sub-region, although S. cerevisiae populations did not differ significantly across vintages. S. uvarum and S. paradoxus were only identified in the 2017 vintage, demonstrating dynamic wine yeast populations between vintages. We found that the flavonoid profile of Pinot Noir grapes from the same 13 vineyards was also affected by sub-regional terroir. The anthocyanin content was lower and the proportion of methoxylated anthocyanins and flavonols was higher in Pinot Noir grapes from OO, the warmer sub-region as compared to KE, the cooler sub-region. Our study demonstrates that both yeast populations and metabolites associated with the Pinot Noir variety have sub-regional variation within a viticultural area.

Aim: The major focus of this research was to explain the so-called terroir effects that impact grapevine yield components, berry composition, and wine varietal character. To elucidate potential contributors to the terroir effect, vine water status [midday leaf water potential (ψ)] was chosen as a major determinant. The hypothesis of this component of the study was that consistent leaf ψ zones could be identified within vineyard sites and that vine water status would play a major role in vine performance and yield components. Soil texture was anticipated to play a role indirectly through its water-holding capacity.Methods and materials: To test this hypothesis, ten Riesling vineyards representative of each Vintners Quality Alliance of Ontario sub-appellation were selected within the Niagara Peninsula. These vineyards were delineated using global positioning systems and 75–80 sentinel vines were geo-referenced within a sampling grid for data collection. During the 2005–2007 growing seasons, leaf ψ measurements were collected bi-weekly from a subset of these sentinel vines. Data were collected on soil texture and composition, soil water content (SWC), vine performance and yield components. These variables were mapped using geographical information systems software and relationships between them were elucidated.Results: Vineyards were variable in terms of soil texture, composition, nutrition, and moisture. However, in general, few consistent relationships with soil composition variables were found. As hypothesized, consistent leaf ψ zones were identified within vineyards in all three vintages. Some geospatial patterns and relationships were spatially and temporally stable within vineyards. In many cases, spatial distribution of leaf ψ was temporally stable within vineyards despite different weather conditions during each growing season. Spatial trends within vineyards for SWC and leaf ψ were temporally stable over the 3-year period for eight vineyards. Generally, spatial relationships between leaf ψ, SWC, vine size, berry weight and yield were also temporally stable. Some inconsistencies in spatial distribution of variables were attributable to winter injury.Conclusions: Many viticultural variables such as leaf ψ, vine size, berry weight, and yield were spatially variable and, as hypothesized, consistent leaf ψ zones were identified within vineyards in three distinct vintages. Many geospatial patterns and relationships were determined and were temporally stable, and this temporal stability in these variables occurred despite different growing seasons. The strongest relationships were those concerning leaf ψ, SWC, vine size, and berry weight. No consistent relationships were found concerning soil composition. The most consistent soil variables that impacted vine performance and yield components were physical properties, particularly texture.Significance and impact of the study: Soil had some indirect effects, but leaf ψ was more likely a major contributor to the terroir effect, as it had a major impact on vine size, berry weight and yield in many vineyards across multiple vintages. Temporal stability is required for many practical geomatic applications to be initiated in vineyards, but it is also of importance to future research endeavors for this project as well as others.

Introduction The complex interactions of vine cultivars, and localised regional climate associated with specific vineyard sites are important attributes to the concept of terroir and significant contributors to grape maturity and wine sensory profiles. An improved understanding of the influence of each factor and their interactions is a challenging conundrum, and will enable more efficient production targeting specific wine styles. Objectives To characterise the metabolic flux of grape berries and resulting wines to characterise the relative impact of site specific climate, cultivar, and grape maturity based upon berry sugar accumulation models that consistently target specific wine styles. Methods A spatial and temporal study of grape and wine composition was undertaken for two important cultivars in two distinct regions of New South Wales. Measures of composition and wine sensory ratings were simultaneously analysed using a multiblock algorithm taking advantage of the ANOVA framework to identify important contributions to wine style arising from grape maturity, vineyard site and cultivar. Results A consistent flux of grape and wine constituents is evident for wine made from sequentially harvested grapes from the same vineyard with increasing levels of grape maturity. Contributions of region and vineyard site to wine style could also be elucidated. Differences in metabolite flux in grapes and resulting wines between cultivars growing in similar conditions are evident. Conclusions The combination of a metabolomics and multiblock data decomposition approach may be successfully used to profile and elucidate the contribution of abiotic factors to grape and wine composition and provide improved understanding of the terroir concept.