BACKGROUND: Genus Fragaria (family - Rosaceae), popular edible berry fruits with delicious flavor and considerable health benefits has many wild relatives in the Himalayan region. OBJECTIVES: The objective of the study was to characterize variability in morphology, physicochemical properties, phytochemicals, and antioxidant activity in wild Himalayan Strawberry, Fragaria nubicola along the ripening stages and among the genotypes. METHOD: Morphological and physicochemical properties, thiamine, carotenes, total phenolic content, phenolic compounds, total flavonoids, flavonols, tannins, anthocyanins, and antioxidant activity (ABTS, DPPH, and FRAP assays) were determined in four ripening stages of berry fruits. RESULTS: Morphological attributes (diameter, length, volume, and fresh weight of berries) have shown considerable variations among the genotypes and increased significantly (p