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Background: Overstrain of the superficial digital flexor tendon (SDFT) is a common Thoroughbred racehorse limb injury requiring treatment. Objectives: To determine whether treatment of SDFT lesions in flat Thoroughbred racehorses with autologous bone marrow‐derived mesenchymal stem cells (BM‐MSCs) or allogenic adipose‐derived mesenchymal stem cells (A‐MSCs) is associated with improved likelihood of returning to racing, when compared to racehorses managed with a controlled exercise rehabilitation program (CERP) alone. Study design: Retrospective cohort study combining clinical treatment records with race records. Methods: A total of 213 Thoroughbred racehorses were identified. All were prescribed the same 12‐month CERP and 66 also received intralesional BM‐MSC and 17 A‐MSC treatment. Follow‐up was a minimum of 2 years after return to full race training. Multivariable logistic regression models were used to investigate associations between the treatments and the likelihood of returning to racing and completing five or more (C5+) races post‐injury. Results: Compared to CERP alone, BM‐MSC treatment was associated with increased odds of returning to racing (OR 3.19; 95% CI 1.55–6.81) and C5+ races post‐injury (OR 2.64; 95% CI 1.32–5.33). Older age and increasing lesion length were associated with a reduced likelihood of returning to racing. Male sex and increased number of pre‐injury starts were associated with increased odds of returning to racing. There was no observed increased likelihood of return to racing or C5+ races associated with treatment with A‐MSCs compared to CERP alone. Main limitations: Due to the retrospective nature of the study it was not possible to ascertain how strictly the CERP was followed. Due to the novelty of the method, the A‐MSC treatment group included a limited number of horses. Conclusions: In the study population, intralesional BM‐MSC treatment was significantly associated with an increased likelihood of returning to racing and C5+ races post‐injury compared to CERP alone. Intralesional A‐MSC showed no significant association between treatment and the two investigated outcomes. [ABSTRACT FROM AUTHOR]

Background: Little is known about potential differences in the left recurrent laryngeal nerve (Lrln) and left cricoarytenoideus dorsalis (LCAD) muscle between domestic and feral horse populations. If a difference exists, feral horses may provide a useful control population for research related to recurrent laryngeal neuropathy (RLN) and increase our understanding of potential population pressures influencing the incidence RLN. Objectives: The objective of this study was to compare the Lrln and LCAD of domestic and feral horses using histological and immunohistochemical techniques (IHC). Methods: Sixteen horses, domestic (n = 8) and feral (n = 8), without clinical or ancillary examinations that were processed at an abattoir had the Lrln and LCAD muscle harvested immediately following death. Carcass weights were recorded. Subjective and morphometric histologic assessment were performed on Lrln sections. The LCAD was assessed for myosin heavy chain (fibre type proportion, diameter and grouping using IHC. Results: Fibre‐type grouping consistent with RLN was seen in both groups. Regenerating fibre clusters were more common in domestic compared to feral horses (p = 0.04). No other histologic differences occurred between groups. Muscle fibre typing demonstrated a lower mean percentage of type IIX fibres in the feral group compared to the domestic group (p = 0.03). There was no difference in type I or IIA proportions or mean diameter of any fibre type between the groups. Conclusions: The domestic population showed evidence of nerve regeneration suggesting RLN in this group, yet this was not supported by the higher proportion of type IIX muscle fibres compared to the feral population. Further evaluation to clarify the significance and wider occurrence of the differences is indicated. [ABSTRACT FROM AUTHOR]

Background: Laser salpingopharyngostomy has been used as an adjunct to primary medical therapy allowing for topical debridement, topical treatment or to potentially alter the guttural pouch environment in horses with guttural pouch disease. Objectives: To evaluate the effect of a laser salpingopharyngostomy on the guttural pouch environment in healthy horses. Study design: Experimental study. Methods: In six adult Standardbred female horses, under standing sedation a stab incision was made ventral to the sternocephalicus tendon and a customised trocar inserted into the medial guttural pouch compartment. Baseline (day 0) temperature, humidity, oxygen (O2) and carbon dioxide (CO2) levels were recorded. A laser salpingopharyngostomy was subsequently made into the right guttural pouch. The guttural pouch environment was re‐sampled as above on days 7 and 14 to detect differences in the local environment of the guttural pouch caused by the laser salpingopharyngostomy. Results: The guttural pouch environment was altered by the laser salpingopharyngostomy. Baseline recorded values within the guttural pouch were highly constant within each horse. Post laser salpingopharyngostomy there was considerable variation within the guttural pouch between horse and during the period of sampling. Specifically, CO2 levels post laser salpingopharyngostomy appeared to be linked directly to the horse's respiratory pattern with peaks and troughs recorded post laser salpingopharyngostomy. The variation in CO2 was significantly different at days 7 and 14 post laser salpingopharyngostomy compared with baseline levels (day 7 = 6 mm Hg, day 14 = 8.83 mm Hg). Oxygen levels were also more varied during sampling compared with baseline (day 7 = 3.4%, day 14 = 1.83%). There was no change in humidity or temperature during the study. Main limitations: This study was conducted on horses with no evidence of guttural pouch abnormalities or disease. Conclusions: Laser salpingopharyngostomy alters the guttural pouch environment and this may be useful in the treatment of guttural pouch disease where this alteration is desirable. [ABSTRACT FROM AUTHOR]

OBJECTIVE To describe left recurrent laryngeal neurectomy (LRLn) performed under standing sedation and evaluate the effect of LRLn on upper respiratory tract function using a high-speed treadmill test (HST). We hypothesized that (1) unilateral LRLn could be performed in standing horses, resulting in ipsilateral arytenoid cartilage collapse (ACC); and (2) HST after LRLn would be associated with alterations in upper respiratory function consistent with dynamic ACC. ANIMALS 6 Thoroughbred horses. METHODS The horses were trained and underwent a baseline HST up to 14 m/s at 5% incline until fatigue. Evaluation included; airflow, pharyngeal and tracheal pressures, and dynamic upper respiratory tract endoscopy. Trans-laryngeal impedance (TLI) and left-to-right quotient angle ratio (LRQ) were calculated after testing. The following day, standing LRLn was performed in the mid-cervical region. A HST was repeated within 4 days after surgery. RESULTS Standing LRLn was performed without complication resulting in Havemayer grade 4 ACC at rest (complete paralysis) and Rakestraw grade C or D ACC (collapse up to or beyond rima glottis midline) during exercise. Increasing treadmill speed from 11 to 14 m/s increased TLI (P < .001) and reduced LRQ (P < .001). Neurectomy resulted in an increase in TLI (P = .021) and a reduction in LRQ (P < .001). CLINICAL RELEVANCE Standing LRLn induces laryngeal hemiplegia that can be evaluated using a HST closely after neurectomy. Standing LRLn may be useful for future prospective evaluations of surgical interventions for laryngeal hemiplegia. [ABSTRACT FROM AUTHOR]

Simple Summary: Early detection of racehorses at risk of stress fracture is key to reducing the number of horses with catastrophic fractures while racing. Bone changes are often visible in the limbs of Thoroughbred racehorses in work, particularly in the fetlock region. However, it is currently unknown whether some of these changes indicate an impending fracture or are a healthy adaptation to high-speed exercise. This study looks at imaging and gross changes in a specific area (parasagittal grooves (PSGs) of the cannon bone) and the utility of X-ray, computed tomography (CT) and magnetic resonance imaging (MRI) to detect the changes. All fetlock joints were assessed from twenty horses that died during racing or training, including horses with and without fetlock fracture. Overall, X-ray was poor for detecting PSG changes. Some PSG changes on CT and MRI were common in Thoroughbred racehorses and possibly represent normal bone adaptation when seen in clinical cases. However, certain CT and MRI findings were more prevalent in horses with a fracture, possibly indicating microdamage accumulation and increased risk of fracture. Bilateral advanced imaging is recommended in clinical cases of suspected fetlock pathology. (1) Background: Parasagittal groove (PSG) changes are often present on advanced imaging of racing Thoroughbred fetlocks and have been suggested to indicate increased fracture risk. Currently, there is limited evidence differentiating the imaging appearance of prodromal changes in horses at risk of fracture from horses with normal adaptive modelling in response to galloping. This study aims to investigate imaging and gross PSG findings in racing Thoroughbreds and the comparative utility of different imaging modalities to detect PSG changes. (2) Methods: Cadaver limbs were collected from twenty deceased racing/training Thoroughbreds. All fetlocks of each horse were examined with radiography, low-field magnetic resonance imaging (MRI), computed tomography (CT), contrast arthrography and gross pathology. (3) Results: Horses with fetlock fracture were more likely to have lateromedial PSG sclerosis asymmetry and/or lateral PSG lysis. PSG lysis was not readily detected using MRI. PSG subchondral bone defects were difficult to differentiate from cartilage defects on MRI and were not associated with fractures. The clinical relevance of PSG STIR hyperintensity remains unclear. Overall, radiography was poor for detecting PSG changes. (4) Conclusions: Some PSG changes in Thoroughbred racehorses are common; however, certain findings are more prevalent in horses with fractures, possibly indicating microdamage accumulation. Bilateral advanced imaging is recommended in racehorses with suspected fetlock pathology. [ABSTRACT FROM AUTHOR]

Simple Summary: Research into the advanced imaging appearance of Thoroughbred racehorse fetlocks is receiving increased attention in an effort to better understand and potentially reduce the occurrence of catastrophic fracture in these horses. Studies in this area commonly use cadaver equine limbs from racehorses and a freeze-thaw process prior to imaging. The low-field magnetic resonance imaging (MRI) appearance of the bones of the fetlock joint is of particular interest in the diagnosis of impending fracture in this location. However, little is known about the effect of the freeze-thaw process on the appearance of certain signal changes ("STIR hyperintensity") seen commonly in racehorses. This study compares the low-field MRI appearance of short tau inversion recovery (STIR) hyperintensity in the bones of cadaver fetlocks from Thoroughbreds in race training, before and after a freeze-thaw protocol. Fifteen cadaver fetlocks with abnormal bone signal were included in the study. Blinded and unblinded statistical comparisons were made. No overall clinical or statistical difference was detected in intensity and distribution of the STIR signal before and after freeze-thaw. This indicates that the MRI appearance of STIR hyperintensity in freeze-thawed cadaver fetlocks can be considered representative of the appearance of pathology in the recently euthanized horse. This is important information to further advance research in the area of Thoroughbred racing fatality reduction. Equine advanced imaging research involving racehorse fetlock pathology commonly uses cadaver limbs and a freeze-thaw process. The presence of short tau inversion recovery (STIR) signal intensity in the distal third metacarpal/metatarsal bone is of particular interest and may be clinically relevant in the diagnosis of horses at risk of fracture. However, little is known about the effect of the freeze-thaw process on the MRI appearance of STIR hyperintensity in these bones. This study compares the low-field MRI appearance of the distal third metacarpal/metatarsal bone from cadaver limbs of Thoroughbreds in race training before and after a freeze-thaw protocol. Blinded and unblinded comparisons were made using objective SNR values and subjective grading. Fifteen cadaver limbs with STIR hyperintensity in the distal third metacarpal/metatarsal bone were included. No overall clinical or statistical significance was detected in STIR signal intensity and distribution after freeze-thaw. Three limbs from one horse had individual changes in STIR hyperintensity that were hypothesized to be attributable to ante-mortem haemodynamic abnormalities caused by anaesthesia. These results indicate that the distribution and intensity of STIR hyperintensity in freeze-thawed cadaver fetlocks can be considered representative of the appearance of pathology in the recently euthanized horse. However, care should be taken with horse selection and handling of the cadaver limbs to ensure reliable appearance of STIR signal after freeze-thaw. [ABSTRACT FROM AUTHOR]