Search

The frequent outbreaks of the AIDS (Acquired Immune Deficiency Syndrome) pandemic and the limited availability of anti‐Human Immunodeficiency Virus (HIV) drugs highlight the urgent need to develop new antiviral drugs. A detailed understanding of the interactions between TAR‐Binding Proteins (TBP) and RNA will facilitate the discovery of new anti‐AIDS drugs. In order to characterize and explore the key interactions between RNA and TBP, we focused on the wild type (WT) and three mutant TBPs (TBP6.9, TBP6.7, and TBP6.3) with RNA, multiple molecular dynamics simulation and energy computation were performed. The results showed that 12 key residues played a major role in the interaction between TBP and RNA. The mutated residues of TBP changed the interaction between their surrounding residues and RNA, thus affecting the binding of TBP to RNA. In addition, structural and energy analyses showed that in contrast with WT TBP‐RNA complex, the mutated residues had little effect on the backbone structure of TBP, but changes in the van der Waals interactions and electrostatic interaction associated with the side chains are responsible for the altered the binding between three mutant TBPs and RNA complexes. The discovery of TBP‐RNA recognition mechanism in our work provides some useful insights and new opportunities for the development of anti‐aids drugs. [ABSTRACT FROM AUTHOR]

The resistance of HIV-1 protease (PR) mutations seriously affects the efficiency of current drugs in the clinical treatment of AIDS. Therefore, it is important to understand the resistance mechanism of mutated residues for the development of PR inhibitors. In this work, the mechanism of G48M mutant PR to inhibitors Saquinavir and Atazanavir was studied by using molecular dynamics simulation combined with free energy prediction. The analysis results showed that, compared with the WT PR, the distance of the flap tip from the protease catalytic binding site and the dihedral angles in the flap regions in the mutant type complexes was changed. These changes resulted in a significant reduction in the occupancy of most of the hydrogen bonds in the mutant complex and the interaction energy of key residues with the inhibitor, which is the main reason for the resistance of the mutation to these inhibitors. This study can provide important guidance for the design of effective inhibitors against HIV-1 PR. [ABSTRACT FROM AUTHOR]