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The aim of this research was to investigate the effect of vibration applications on muscle strength and balance. As the subjects to the research, nineteen male sport science students (age: 21.45±2.16 years, height: 177.55±7.24 cm, body mass: 71.62±11.02 kg) voluntarily participated in the study. The students were exposed to vibration in squat exercise position before they were exposed to vibration at 25Hz, 50Hz frequency and control (no-vibration) on three different application days. Before and after the vibration exposure, jump tests and balance tests were administered right after the 5th, 10th and 15th min in the wake of the vibration. A statistically significant difference was found in active and squat jump heights administered following the 25Hz and 50 Hz frequency applications (p[less than or equal to]0.05). No significant differences were found in the jump heights in control group (p>0.05). While there was no significant difference found in balance tests performed after 25Hz and 50Hz vibration applications (p>0.05), a significant difference was found after the control application (p[less than or equal to]0.05). As a result, there was an increase in jump heights following the acute vibration application however, no change was seen in balance scores. Practicing acute vibration applications can be recommended to increase jump height especially before the competitions and applications.