1. Bioassay-Guided Isolation and Identification of Cytotoxic Compounds from Gymnosperma glutinosum Leaves.
- Author
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Quintanilla-Licea, Ramiro, Morado-Castillo, Rolando, Gomez-Flores, Ricardo, Laatsch, Hartmut, Verde-Star, María Julia, Hernández-Martínez, Humberto, Tamez-Guerra, Patricia, Tamez-Guerra, Reyes, and Rodríguez-Padilla, Cristina
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BIOLOGICAL assay , *HEXANE , *ASTERACEAE , *MASS spectrometry - Abstract
Bioassay-guided fractionation of hexane extracts of Gymnosperma glutinosum (Asteraceae) leaves, collected in North Mexico, afforded the known compounds hentriacontane (1) and (+)-13S,14R,15-trihydroxy-ent-labd-7-ene (2), as well as the new ent-labdane diterpene (-)-13S,14R,15-trihydroxy-7-oxo-ent-labd-8(9)-ene (3). In addition, D-glycero-D-galactoheptitol (4) was isolated from the methanolic extract of this plant. Their structures were established on the basis of high-field 1D- and 2D NMR methods supported by HR-MS data. The cytotoxic activity was determined by using the in vitro L5178Y-R lymphoma murine model. Hentriacontane (1) and the new ent-labdane 3 showed weak cytotoxicity, whereas the ent-labdane 2 showed significant (p < 0.05) and concentration dependent cytotoxicity (up to 78%) against L5178Y-R cells at concentrations ranging from 7.8 to 250 μg/mL. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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