1. Anti-Epstein-Barr Viral Agents from the Medicinal Herb-Derived Fungus Alternaria alstroemeriae Km2286.
- Author
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Chang CH, Lee YC, Hsiao G, Chang LK, Chi WC, Cheng YC, Huang SJ, Wang TC, Lu YS, and Lee TH
- Subjects
- Humans, Molecular Structure, Tenuazonic Acid chemistry, Alternaria chemistry, Alternaria isolation & purification, Atriplex microbiology, Epstein-Barr Virus Infections virology, Herpesvirus 4, Human drug effects, Perylene chemistry, Perylene isolation & purification, Perylene pharmacology, Plants, Medicinal microbiology, Quinones chemistry, Quinones isolation & purification, Quinones pharmacology, Anti-Inflammatory Agents chemistry, Anti-Inflammatory Agents isolation & purification, Anti-Inflammatory Agents pharmacology, Antiviral Agents chemistry, Antiviral Agents isolation & purification, Antiviral Agents pharmacology
- Abstract
Chromatographic separation on the liquid-state fermented products produced by the fungal strain Alternaria alstroemeriae Km2286 isolated from the littoral medicinal herb Atriplex maximowicziana Makino resulted in the isolation of compounds 1 - 9 . Structures were determined by spectroscopic analysis as four undescribed perylenequinones, altertromins A-D ( 1 - 4 ), along with altertoxin IV ( 5 ), altertoxin VIII ( 6 ), stemphyperylenol ( 7 ), tenuazonic acid ( 8 ), and allo -tenuazonic acid ( 9 ). Compounds 1 - 6 exhibited antiviral activities against Epstein-Barr virus (EBV) with EC
50 values ranging from 0.17 ± 0.07 to 3.13 ± 0.31 μM and selectivity indices higher than 10. In an anti-neuroinflammatory assay, compounds 1 - 4 , 6 , and 7 showed inhibitory activity of nitric oxide production in lipopolysaccharide-induced microglial BV-2 cells, with IC50 values ranging from 0.33 ± 0.04 to 4.08 ± 0.53 μM without significant cytotoxicity. This is the first report to describe perylenequinone-type compounds with potent anti-EBV and anti-neuroinflammatory activities.- Published
- 2022
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