1. Biodegradation of the nitroaromatic herbicide dinoseb (2-sec-butyl-4,6-dinitrophenol) under reducing conditions.
- Author
-
Kaake RH, Crawford DL, and Crawford RL
- Subjects
- Acetylation, Bacteria, Aerobic metabolism, Biodegradation, Environmental, Cell Extracts chemistry, Chromatography, Culture Media chemistry, Glycerol chemistry, Molecular Weight, Quinones chemistry, Quinones isolation & purification, Quinones metabolism, 2,4-Dinitrophenol analogs & derivatives, Dinitrophenols metabolism, Herbicides metabolism, Soil Microbiology
- Abstract
The degradation pathway for dinoseb (2-sec-butyl-4,6-dinitrophenol) under reducing conditions was investigated. Cultures were inoculated with a dinoseb-degrading anaerobic enrichment culture used in field studies. Biotransformation intermediates were extracted with ethyl acetate and analyzed by high pressure liquid chromatography, gas chromatography, and mass spectrometry. Dinoseb degradation involves reduction of the nitro groups to amino groups followed by replacement with hydroxyl groups. Depending on the pH and redox potential in the culture, these intermediates may exist as quinones or hydroquinones.
- Published
- 1995
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