4 results on '"Friedemann, Thomas"'
Search Results
2. Neuroprotective Effect of Coptis chinensis in MPP and MPTP-Induced Parkinson's Disease Models.
- Author
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Friedemann, Thomas, Ying, Yue, Wang, Weigang, Kramer, Edgar R., Schumacher, Udo, Fei, Jian, and Schröder, Sven
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ANALYSIS of variance , *HERBAL medicine , *CHINESE medicine , *PARKINSON'S disease , *RESEARCH funding , *STATISTICS , *PLANT extracts , *DATA analysis , *NEUROPROTECTIVE agents - Abstract
The rhizome of Coptis chinensis is commonly used in traditional Chinese medicine alone or in combination with other herbs to treat diseases characterized by causing oxidative stress including inflammatory diseases, diabetes mellitus and neurodegenerative diseases. In particular, there is emerging evidence that Coptis chinensis is effective in the treatment of neurodegenerative diseases associated with oxidative stress. Hence, the aim of this study was to investigate the neuroprotective effect of Coptis chinensis in vitro and in vivo using MPP and MPTP models of Parkinson's disease. MPP treated human SH-SY5Y neuroblastoma cells were used as a cell model of Parkinson's disease. A 24h pre-treatment of the cells with the watery extract of Coptis chinensis significantly increased cell viability, as well as the intracellular ATP concentration and attenuated apoptosis compared to the MPP control. Further experiments with the main alkaloids of Coptidis chinensis, berberine, coptisine, jaterorrhizine and palmatine revealed that berberine and coptisine were the main active compounds responsible for the observed neuroprotective effect. However, the full extract of Coptis chinensis was more effective than the tested single alkaloids. In the MPTP-induced animal model of Parkinson's disease, Coptis chinensis dose-dependently improved motor functions and increased tyrosine hydroxylase-positive neurons in the substantia nigra compared to the MPTP control. Based on the results of this work, Coptis chinensis and its main alkaloids could be considered potential candidates for the development of new treatment options for Parkinson's disease. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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3. Neuroprotective Activity of Coptisine from Coptis chinensis (Franch).
- Author
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Friedemann, Thomas, Schumacher, Udo, Yi Tao, Leung, Alexander Kai-Man, and Schröder, Sven
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CARDIOVASCULAR disease treatment , *TREATMENT of neurodegeneration , *REACTIVE oxygen species , *ALKALOIDS , *ANALYSIS of variance , *APOPTOSIS , *BIOLOGICAL transport , *CELL culture , *CHROMATOGRAPHIC analysis , *ENZYME-linked immunosorbent assay , *GENE expression , *HERBAL medicine , *CHINESE medicine , *POLYMERASE chain reaction , *RESEARCH funding , *DESCRIPTIVE statistics , *IN vitro studies - Abstract
Coptis chinensis rhizomes (CR) are one important ingredient of traditional Chinese herbal formulas such as San-Huang-Xie-Xin- Tang which is used for treatment of cardiovascular and neurodegenerative diseases. Recent studies suggest that the extract of CR might be a potential therapeutic agent for amelioration of neurological disorders associated with oxidative stress. In the present study we aimed at revealing the main active compound(s) of the CR extract and at investigating the mechanism of action. Four main alkaloids of the CR extract (berberine, coptisine, jatrorrhizine, and palmatine) were selected for this study. Results showed that out of those alkaloids only pretreatment with coptisine significantly attenuated tert-butylhydroperoxide induced reduction of cell viability, increased rate of apoptosis, and declined mitochondrial membrane potential. Elisa assay and quantitative real-time PCR analyses revealed that thioredoxin-interacting protein (TXNIP) gene expression was downregulated by coptisine, which could explain the neuroprotective effect, hypothetically, by strengthening the thioredoxin defense system against oxidative stress and attenuation of apoptosis signal-regulating kinase (Ask1) mediated apoptotic signaling. A comparison between coptisine and CR extract identified coptisine as the main single component responsible for the neuroprotective effect. Based on the results the CR extract and coptisine are promising candidate agents for prevention or improvement of diabetic neuropathy and neuro degenerative disorders. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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4. Coptis chinensis Franch. exhibits neuroprotective properties against oxidative stress in human neuroblastoma cells.
- Author
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Friedemann, Thomas, Otto, Benjamin, Klätschke, Kristin, Schumacher, Udo, Yi Tao, Kai-Man Leung, Alexander, Efferth, Thomas, and Schröder, Sven
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ALKALOIDS , *APOPTOSIS , *CELLS , *HERBAL medicine , *CHINESE medicine , *NEUROBLASTOMA , *POLYMERASE chain reaction , *OXIDATIVE stress , *NEUROPROTECTIVE agents , *IN vitro studies - Abstract
Ethnopharmacological relevance The dried rhizome of Coptis chinensis Franch. (family Ranunculaceae) is traditionally used in Chinese medicine for the treatment of inflammatory diseases and diabetes. Recent studies showed a variety of activities of Coptis chinensis Franch. alkaloids, including neuroprotective, neuroregenerative, anti-diabetic, anti-oxidative and anti-inflammatory effects. However, there is no report on the neuroprotective effect of Coptis chinensis Franch. watery extract against tert-butylhydroperoxide (t-BOOH) induced oxidative damage. The aim of the study is to investigate neuroprotective properties of Coptis chinensis Franch. rhizome watery extract (CRE) and to evaluate its potential mechanism of action. Materials and methods Neuroprotective properties on t-BOOH induced oxidative stress were investigated in SH-SY5Y human neuroblastoma cells. Cells were pretreated with CRE for 2 h or 24 h followed by 2 h of treatment with t-BOOH. To evaluate the neuroprotective effect of CRE, cell viability, cellular reactive oxygen species (ROS), mitochondrial membrane potential (MMP) and the apoptotic rate were determined and microarray analyses, as well as qRT-PCR analyses were conducted. Results Two hours of exposure to 100 µM t-BOOH resulted in a significant reduction of cell viability, increased apoptotic rate, declined mitochondrial membrane potential (MMP) and increased ROS production. Reduction of cell viability, increased apoptotic rate and declined mitochondrial membrane potential (MMP) could be significantly reduced in cells pretreated with CRE (100 µg/ml) for 2 h or 24 h ahead of t-BOOH exposure with the greatest effect after 24 h of pretreatment; however ROS production was not changed significantly. Furthermore, microarray analyses revealed that the expressions of 2 genes; thioredoxin-interacting protein (TXNIP) and mitochondrially encoded NADH dehydrogenase 1, were significantly regulated. Down regulation of TXNIP was confirmed by qRT-PCR. Conclusion Due to its neuroprotective properties CRE might be a potential therapeutic agent for the prevention or amelioration of diseases like diabetic neuropathy and neurodegenerative disorders like Alzheimer and Parkinsons disease. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
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