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Start Over Author "Samy B Khalil" Topic hepatitis c
2 results on '"Samy B Khalil"'

1. Establishment of Hybrid Cell Lines Producing Monoclonal Antibodies to a Synthetic Peptide from the E1 Region of the Hepatitis C Virus

Author

Gamal Esmat, Amr Helmy, Ashraf A Tabll, Samy B. Khalil, Reem El-Shenawy, Mostafa K. El-Awady, and Abdel Fattah Attallah

Subjects
Adult, Male, Adolescent, medicine.drug_class, Hepatitis C virus, Blotting, Western, Clinical Biochemistry, Immunology, Enzyme-Linked Immunosorbent Assay, Peptide, Biology, Monoclonal antibody, medicine.disease_cause, Cell Line, Serology, Mice, Viral Envelope Proteins, Antigen, medicine, Animals, Humans, Immunology and Allergy, Serologic Tests, chemistry.chemical_classification, Mice, Inbred BALB C, Hybridomas, Antibodies, Monoclonal, virus diseases, Middle Aged, Hepatitis C, Virology, Molecular biology, digestive system diseases, Blot, Medical Laboratory Technology, chemistry, Cell culture, biology.protein, Female, Hepatitis C Antigens, Antibody, Peptides
Abstract

We aimed at establishing hybridoma cells secreting monoclonal antibodies (mAbs) against E1 synthetic peptide of HCV. BALB/c mice were immunized with HCV E1-synthetic peptide (GHRMAWDMM) and its spleenocytes were fused with the P3NS1 myeloma cell line. Two highly reactive and specific mAbs (10C7 IgG2b mAb, and 10B2 IgG1 mAb) were generated. The target HCV E1 antigen was identified at approximately 38 kDa in serum of infected individuals. A newly developed ELISA detected the target antigen in 90% of sera from HCV RNA infected individuals with a specificity of 84%. So, the generated mAbs may provide promising probes for serodiagnosis of HCV infection.

Published
2007
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2. Synthetic peptide-based immunoassay as a supplemental test for HCV infection

Author

Samy B Khalil, Said A. Goueli, Dalia Galal, Maha A. El-Demellawy, and Mostafa K. El Awady

Subjects
Hepatitis C virus, Clinical Biochemistry, Population, medicine.disease_cause, Biochemistry, Sensitivity and Specificity, Virus, medicine, Humans, Mass Screening, education, Mass screening, Immunoassay, Viral Structural Proteins, education.field_of_study, biology, medicine.diagnostic_test, Viral Core Proteins, Biochemistry (medical), Reproducibility of Results, General Medicine, Hepatitis C, Hepatitis C Antibodies, medicine.disease, Virology, Immunology, biology.protein, Egypt, Viral disease, Antibody, Peptides
Abstract

Introduction: Hepatitis C virus (HCV) is a single strand RNA hepatotrophic virus infecting 170 millions around the world and 20% of Egyptian blood donors. Although there has been significant improvement in the enzyme immunoassays (EIAs) in population screening of HCV infection, the development of a low variability, easy to automate and inexpensive supplemental test to support the current immunoassays was of a major concern to several laboratories. Objectives: In the current study, we embarked on a systematic study to analyze by DNA sequencing several HCV isolates to identify conserved core protein sequences and perform explorative analysis of five synthetic peptides from the core/E1 region in anti-HCV antibody assays. Methods: We designed four synthetic-core specific peptides and an E1-specific peptide. These peptides were used to screen HCV antibodies in sera of 100 HCV positive patients and 100 HCV negative subjects and compared the results with those obtained by the commercial systems based on second and third generation enzyme-linked immunosorbent assays. Results: Our results showed that all peptides detect HCV antibodies in infected sera to varying degrees. The synthetic peptide (a.a. 21–40) of the core protein had 99% sensitivity, 100% specificity and was highly reproducible. Conclusion: The above findings make this core peptide a candidate product for developing a supplemental test for chronic HCV infection in the Egyptian population.

Published
2002

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