1. A "signal on" protection-displacement-hybridization-based electrochemical hepatitis B virus gene sequence sensor with high sensitivity and peculiar adjustable specificity.
- Author
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Li F, Xu Y, Yu X, Yu Z, He X, Ji H, Dong J, Song Y, Yan H, and Zhang G
- Subjects
- Biosensing Techniques instrumentation, Electrodes, Gold chemistry, Hepatitis B virus isolation & purification, Humans, Limit of Detection, Methylene Blue chemistry, Nucleic Acid Hybridization, Point Mutation, Electrochemical Techniques instrumentation, Hepatitis B virology, Hepatitis B virus genetics
- Abstract
One "signal on" electrochemical sensing strategy was constructed for the detection of a specific hepatitis B virus (HBV) gene sequence based on the protection-displacement-hybridization-based (PDHB) signaling mechanism. This sensing system is composed of three probes, one capturing probe (CP) and one assistant probe (AP) which are co-immobilized on the Au electrode surface, and one 3-methylene blue (MB) modified signaling probe (SP) free in the detection solution. One duplex are formed between AP and SP with the target, a specific HBV gene sequence, hybridizing with CP. This structure can drive the MB labels close to the electrode surface, thereby producing a large detection current. Two electrochemical testing techniques, alternating current voltammetry (ACV) and cyclic voltammetry (CV), were used for characterizing the sensor. Under the optimized conditions, the proposed sensor exhibits a high sensitivity with the detection limit of ∼5fM for the target. When used for the discrimination of point mutation, the sensor also features an outstanding ability and its peculiar high adjustability., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2016
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