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The objective of this study was to determine the nutritional requirement of digestible Trp for white egg layers in the period from 60 to 76 wk of age. A total of 180 Dekalb White layers with an average weight of 1,345 kg were distributed in a completely randomized design with 5 increasing levels of digestible Trp (0.167, 0.175, 0.183, 0.191, and 0.199%), 6 replicates, and 6 birds per experimental unit. The diets of the treatments contained 0.759% of digestible Lys, which was obtained using the prediction equation of the Brazilian Tables for Poultry and Swine, and each treatment consisted of a digestible Trp-to-Lys ratio of 22, 23, 24, 25, or 26%. The digestible Trp levels in the diet had a quadratic effect on egg production, egg mass, and conversion per mass and per dozen eggs, and a linear effect on egg weight. In conclusion, a diet with 0.192% digestible Trp, in a 25.33% ratio with digestible Lys or 212 mg/bird per day, can be recommended for white egg layers of 60 to 76 wk of age. [ABSTRACT FROM AUTHOR]

Oxidative stress significantly compromises the production efficiency of laying hens. It has been reported in literature that selenium (Se) in poultry diets has a positive effect on mitigating these effects. This study has been carried out to evaluate the effects of Se supplementation in feeds, from either an inorganic or a hydroxy-selenomethionine (OH-SeMet) source, on the performance and physiological traits of 50- to 70-wk-old Dekalb Brown laying hens under heat stress, and on their egg quality after different storage durations. The treatments consisted in supplementing 0.3 ppm of Se as sodium selenite (SS ; 45%–0.7g/ton) or OH-SeMet (2%–15g/ton) in twelve 16-bird replicates. Supplementation with OH-SeMet resulted in a better performance of the laying hens than with SS: −5% feed conversion ratio and +3.6% of egg mass. A reduction in egg quality was observed with prolonged egg storage, which was mitigated with the use of OH-SeMet in laying hen diets. The use of OH-SeMet increased the antioxidant capacity of the birds, which showed higher glutathione peroxidase levels in the blood, kidneys, liver, and intestinal mucosa, in addition to a higher Se content in the eggs and a greater bone resistance. Thus, supplementing feeds with 0.3 ppm of OH-SeMet to 50- to 70-wk-old semi-heavy laying hens enhances their antioxidant capacity and leads to a higher egg quality and productivity than SS supplementation. [ABSTRACT FROM AUTHOR]