1. Amomum tsao-ko suppresses lipopolysaccharide-induced inflammatory responses in RAW264.7 macrophages via Nrf2-dependent heme oxygenase-1 expression.
- Author
-
Li B, Choi HJ, Lee DS, Oh H, Kim YC, Moon JY, Park WH, Park SD, and Kim JE
- Subjects
- Amomum, Animals, Anti-Inflammatory Agents, Antioxidants, Cells, Cultured, I-kappa B Proteins metabolism, Inflammation chemically induced, Inflammation drug therapy, Inflammation genetics, Lipopolysaccharides pharmacology, Mice, NF-E2-Related Factor 2, NF-KappaB Inhibitor alpha, NF-kappa B metabolism, Neuroprotective Agents, Phosphorylation drug effects, Plant Extracts therapeutic use, Gene Expression drug effects, Heme Oxygenase-1 genetics, Heme Oxygenase-1 metabolism, Inflammation Mediators metabolism, Macrophages metabolism, Plant Extracts pharmacology
- Abstract
Amomum tsao-ko Crevost et Lemaire, used as a spice in Asia, is an important source of Chinese cuisine and traditional Chinese medicines. A. tsao-ko is reported to exert a variety of biological and pharmacological activities, including anti-proliferative, anti-oxidative and neuroprotective effects. In this study, NNMBS227, consisting of the ethanol extract of A. tsao-ko, exhibited potent anti-inflammatory activities in RAW264.7 macrophages. We investigated the effect of NNMBS227 in the suppression of pro-inflammatory mediators, including pro-inflammatory enzymes (inducible nitric oxide synthase and cyclooxygenase-2) and cytokines (tumor necrosis factor-α and interleukin-1β) in LPS stimulated macrophages. NNMBS227 also inhibited the phosphorylation and degradation of IκB-α, as well as the nuclear translocation of nuclear factor kappa B (NF-κB) p65 caused by stimulation with LPS. In addition, NNMBS227 induced heme oxygenase (HO)-1 expression through the nuclear translocation of nuclear factor E2-related factor 2 (Nrf2) in macrophages. Using tin protoporphyrin (SnPP), an HO activity inhibitor, we confirmed an association between the anti-inflammatory effects of NNMBS227 and the up-regulation of HO-1. These findings suggest that Nrf2-dependent increases in the expression of HO-1 induced by NNMBS227 conferred anti-inflammatory activities in LPS stimulated RAW264.7 macrophages.
- Published
- 2014
- Full Text
- View/download PDF