1. Rab5c-mediated endocytic trafficking regulates hematopoietic stem and progenitor cell development via Notch and AKT signaling
- Author
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Feng Liu, Peng Lv, Jian Heng, Yifan Zhang, Xinjie Cheng, Lu Wang, and Dongyuan Ma
- Subjects
0301 basic medicine ,Embryology ,Embryo, Nonmammalian ,Endocytic cycle ,Immunostaining ,Biochemistry ,Animals, Genetically Modified ,0302 clinical medicine ,Cell Signaling ,Biology (General) ,Zebrafish ,Hemogenic endothelium ,Notch Signaling ,Staining ,Receptors, Notch ,General Neuroscience ,Gene Expression Regulation, Developmental ,Eukaryota ,Animal Models ,Endocytosis ,Cell biology ,Experimental Organism Systems ,Osteichthyes ,Vertebrates ,293T cells ,Cell lines ,Signal transduction ,Cellular Structures and Organelles ,General Agricultural and Biological Sciences ,Biological cultures ,Research Article ,Signal Transduction ,QH301-705.5 ,Endosome ,Notch signaling pathway ,Endosomes ,Cell fate determination ,Biology ,Green Fluorescent Protein ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Model Organisms ,Animals ,Humans ,Endothelium ,Vesicles ,Progenitor cell ,Protein kinase B ,rab5 GTP-Binding Proteins ,General Immunology and Microbiology ,Embryos ,Organisms ,Biology and Life Sciences ,Proteins ,Cell Biology ,Zebrafish Proteins ,Hematopoietic Stem Cells ,Research and analysis methods ,Luminescent Proteins ,030104 developmental biology ,HEK293 Cells ,Fish ,Specimen Preparation and Treatment ,Fertilization ,Animal Studies ,T-Box Domain Proteins ,Proto-Oncogene Proteins c-akt ,030217 neurology & neurosurgery ,Transcription Factors ,Developmental Biology - Abstract
It is well known that various developmental signals play diverse roles in hematopoietic stem and progenitor cell (HSPC) production; however, how these signaling pathways are orchestrated remains incompletely understood. Here, we report that Rab5c is essential for HSPC specification by endocytic trafficking of Notch and AKT signaling in zebrafish embryos. Rab5c deficiency leads to defects in HSPC production. Mechanistically, Rab5c regulates hemogenic endothelium (HE) specification by endocytic trafficking of Notch ligands and receptor. We further show that the interaction between Rab5c and Appl1 in the endosome is required for the survival of HE in the ventral wall of the dorsal aorta through AKT signaling. Interestingly, Rab5c overactivation can also lead to defects in HSPC production, which is attributed to excessive endolysosomal trafficking inducing Notch signaling defect. Taken together, our findings establish a previously unrecognized role of Rab5c-mediated endocytic trafficking in HSPC development and provide new insights into how spatiotemporal signals are orchestrated to accurately execute cell fate transition., Cell-autonomous Notch signaling regulated by the membrane trafficking protein Rab5c plays an instructive role in hematopoietic stem and progenitor cell specification, while the AKT signaling seems to provide a permissive signal to maintain hemogenic endothelium survival.
- Published
- 2019