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Highlights • The effect of power supply parameters on the Helium emission line intensity have been investigated. • The emission line intensity increases by increasing the voltage amplitude. • The emission line intensity increases by increasing the frequency. • The emission line intensity decreases by increasing the pulse width. • The electron density and temperature have been obtained using the Stark broadening. Abstract In this work, contribution of all the broadenings in the emission line broadening in atmospheric pressure plasma jets (APPJs) have been determined for the cases of 667 nm and 728 nm lines. By fitting the experimental data with Voigt function, the experimental broadening have been obtained. By calculating Instrumental, Doppler, Resonance and Van Der Waals broadenings, and using experimental broadening, Stark broadening have been obtained. Finally, by using Griem's formula, the electron density and temperature have been determined and the effect of supply power parameters, voltage amplitude, frequency and pulse width, on the lines intensities and Stark broadening and the electron density and temperature have been investigated. [ABSTRACT FROM AUTHOR]