30 results on '"Vangronsveld, Jaco"'
Search Results
2. Ectomycorrhizal Protection of Pinus sylvestris against Copper Toxicity
- Author
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van Tichelen, Katia K., Colpaert, Jan V., and Vangronsveld, Jaco
- Published
- 2001
3. Bacteria associated with Zn-hyperaccumulators Arabidopsis halleri and Arabidopsis arenosa from Zn–Pb–Cd waste heaps in Poland as promising tools for bioremediation.
- Author
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Oleńska, Ewa, Małek, Wanda, Wójcik, Małgorzata, Szopa, Sebastian, Swiecicka, Izabela, Aleksandrowicz, Olgierd, Włostowski, Tadeusz, Zawadzka, Weronika, Sillen, Wouter M. A., Vangronsveld, Jaco, Cholakova, Iva, Langill, Tori, and Thijs, Sofie
- Subjects
HYPERACCUMULATOR plants ,BIOREMEDIATION ,ARABIDOPSIS ,PLANT cells & tissues ,COPPER ,RHIZOBACTERIA ,HEAVY metals - Abstract
To identify metal adapted bacteria equipped with traits positively influencing the growth of two hyperaccumulator plant species Arabidopsis arenosa and Arabidopsis halleri, we isolated bacteria inhabiting rhizosphere and vegetative tissues (roots, basal and stem leaves) of plants growing on two old Zn–Pb–Cd waste heaps in Bolesław and Bukowno (S. Poland), and characterized their potential plant growth promoting (PGP) traits as well as determined metal concentrations in rhizosphere and plant tissues. To determine taxonomic position of 144 bacterial isolates, 16S rDNA Sanger sequencing was used. A metabolic characterization of isolated strains was performed in vitro using PGP tests. A. arenosa and A. halleri accumulate high amounts of Zn in their tissues, especially in stem leaves. Among in total 22 identified bacterial taxa, the highest level of the taxonomical diversity (H' = 2.01) was revealed in A. halleri basal leaf endophytes originating from Bukowno waste heap area. The 96, 98, 99, and 98% of investigated strains showed tolerant to Cd, Zn, Pb and Cu, respectively. Generally, higher percentages of bacteria could synthesize auxins, siderophores, and acetoin as well as could solubilize phosphate. Nine of waste heap origin bacterial strains were tolerant to toxic metals, showed in vitro PGP traits and are potential candidates for bioremediation. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
4. Genetic Variation and Heavy Metal Tolerance in the Ectomycorrhizal Basidiomycete Suillus luteus
- Author
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Colpaert, Jan V., Vandenkoornhuyse, Philippe, Adriaensen, Kristin, and Vangronsveld, Jaco
- Published
- 2000
5. Are Grasses Really Useful for the Phytoremediation of Potentially Toxic Trace Elements? A Review
- Author
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Rabelo, Flavio Henrique Silveira, VANGRONSVELD, Jaco, Baker, Alan J. M., van der Ent, Antony, and Alleoni, Luis Reynaldo Ferracciu
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phytoextraction ,tolerance mechanisms ,toxicity ,phytofiltration ,heavy metals ,Poaceae ,phytostabilization ,trace elements uptake - Abstract
The pollution of soil, water, and air by potentially toxic trace elements poses risks to environmental and human health. For this reason, many chemical, physical, and biological processes of remediation have been developed to reduce the (available) trace element concentrations in the environment. Among those technologies, phytoremediation is an environmentally friendly in situ and cost-effective approach to remediate sites with low-to-moderate pollution with trace elements. However, not all species have the potential to be used for phytoremediation of trace element-polluted sites due to their morpho-physiological characteristics and low tolerance to toxicity induced by the trace elements. Grasses are prospective candidates due to their high biomass yields, fast growth, adaptations to infertile soils, and successive shoot regrowth after harvest. A large number of studies evaluating the processes related to the uptake, transport, accumulation, and toxicity of trace elements in grasses assessed for phytoremediation have been conducted. The aim of this review is (i) to synthesize the available information on the mechanisms involved in uptake, transport, accumulation, toxicity, and tolerance to trace elements in grasses; (ii) to identify suitable grasses for trace element phytoextraction, phytostabilization, and phytofiltration; (iii) to describe the main strategies used to improve trace element phytoremediation efficiency by grasses; and (iv) to point out the advantages, disadvantages, and perspectives for the use of grasses for phytoremediation of trace element-polluted soils. This review was supported through financial support for FHSR by the Brazilian Federal Government Agency, Coordination for the Improvement of Higher Education Personnel - CAPES (grant number 1332394), by the National Council for Scientific and Technological Development CNPq (grant number 306403/2013-7), and by the São Paulo Research Foundation – FAPESP (grants number 2012/12894-3, 2012/11859-0, 2014/16731-7, 2014/18735-0, 2015/21562-2, 2017/11299-8, and 2018/07190-3).
- Published
- 2021
6. An Alliance of Trifolium repens — Rhizobium leguminosarum bv. trifolii —Mycorrhizal Fungi From an Old Zn-Pb-Cd Rich Waste Heap as a Promising Tripartite System for Phytostabilization of Metal Polluted Soils.
- Author
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Oleńska, Ewa, Małek, Wanda, Sujkowska-Rybkowska, Marzena, Szopa, Sebastian, Włostowski, Tadeusz, Aleksandrowicz, Olgierd, Swiecicka, Izabela, Wójcik, Małgorzata, Thijs, Sofie, and Vangronsveld, Jaco
- Subjects
RHIZOBIUM leguminosarum ,WHITE clover ,MYCORRHIZAL fungi ,PHYTOREMEDIATION ,HEAVY metals ,VESICULAR-arbuscular mycorrhizas - Abstract
The Bolesław waste heap in South Poland, with total soil Zn concentrations higher than 50,000 mg kg
–1 , 5,000 mg Pb kg–1 , and 500 mg Cd kg–1 , is a unique habitat for metallicolous plants, such as Trifolium repens L. The purpose of this study was to characterize the association between T. repens and its microbial symbionts, i.e., Rhizobium leguminosarum bv. trifolii and mycorrhizal fungi and to evaluate its applicability for phytostabilization of metal-polluted soils. Rhizobia originating from the nutrient-poor waste heap area showed to be efficient in plant nodulation and nitrogen fixation. They demonstrated not only potential plant growth promotion traits in vitro , but they also improved the growth of T. repens plants to a similar extent as strains from a non-polluted reference area. Our results revealed that the adaptations of T. repens to high Zn-Pb-Cd concentrations are related to the storage of metals predominantly in the roots (excluder strategy) due to nodule apoplast modifications (i.e., thickening and suberization of cell walls, vacuolar storage), and symbiosis with arbuscular mycorrhizal fungi of a substantial genetic diversity. As a result, the rhizobia-mycorrhizal fungi- T. repens association appears to be a promising tool for phytostabilization of Zn-Pb-Cd-polluted soils. [ABSTRACT FROM AUTHOR]- Published
- 2022
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7. Molecular Monitoring of SRB Community Structure and Dynamics in Batch Experiments to Examine the Applicability of in situ Precipitation of Heavy Metals for Groundwater Remediation (15 pp)
- Author
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Geets, Joke, Borremans, Brigitte, Vangronsveld, Jaco, Diels, Ludo, and Lelie, Daniël van der
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- 2005
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8. Rehabilitation of the nematode fauna in a phytostabilized, heavily zinc-contaminated, sandy soil
- Author
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Bouwman, Lucas A. and Vangronsveld, Jaco
- Published
- 2004
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9. Amendment-Induced Immobilization of Lead in a Lead-Spiked Soil: Evidence from Phytotoxicity Studies
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Geebelen, Wouter, Vangronsveld, Jaco, Adriano, Domy C., Carleer, Robert, and Clijsters, Herman
- Published
- 2002
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10. Brownfield to green fields: realising wider benefits from practical plant-based contaminant management strategies
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Cundy, Andrew B., Bardos, R.Paul, Puschenreiter, Markus, Mench, Michel, Bert, Valérie, Friesl-Hanl, Wolfgang, Müller, Ingo, Li, X.N., Weyens, Nele, Witters, Nele, and Vangronsveld, Jaco
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contaminated land ,Brownfields ,gentle remediation options ,phytoremediation ,phytomanagement ,heavy metals ,decision support tools - Abstract
Gentle remediation options (GROs) are risk management strategies or technologies involving plant (phyto-), fungi (myco-), and/or bacteria-based methods that result in a net gain (or at least no gross reduction) in soil function as well as effective risk management. GRO strategies can be customised along contaminant linkages, and can generate a range of wider economic, environmental and societal benefits in contaminated land management (and in brownfields management more widely). The application of GROs as practical on-site remedial solutions is still limited however, particularly in Europe and at trace element (typically metal and metalloid) contaminated sites. This paper discusses challenges to the practical adoption of GROs in contaminated land management, and outlines the decision support tools and best practice guidance developed in the European Commission FP7-funded GREENLAND project aimed at overcoming these challenges. The GREENLAND guidance promotes a refocus from phytoremediation to wider GROs- or phyto-management based approaches which place realisation of wider benefits at the core of site design, and where gentle remediation technologies can be applied as part of integrated, mixed, site risk management solutions or as part of “holding strategies” for vacant sites. The combination of GROs with renewables, both in terms of biomass generation but also with green technologies such as wind and solar power, can provide a range of economic and other benefits and can potentially support the return of low-level contaminated sites to productive usage, while combining GROs with urban design and landscape architecture, and integrating GRO strategies with sustainable urban drainage systems and community gardens/parkland (particularly for health and leisure benefits), has large potential for triggering GRO application and in realising wider benefits in urban and suburban systems. Quantifying these wider benefits and value (above standard economic returns) will be important in leveraging funding for GRO application and soft site end-use more widely at vacant or underutilized sites. Further details of the GREENLAND project, and downloadable versions of the GREENLAND decision support tool and GRO best practice guidance, can be found at http://www.greenland-project.eu. The authors are grateful for financial support from the European Commission under the Seventh Framework Programme for Research (FP7-KBBE-266124), and thank the wider GREENLAND consortium and project advisory board for their support and input during the development of the DST and GRO best practice guidance.
- Published
- 2016
11. Seed Endophyte Microbiome of Crotalaria pumila Unpeeled: Identification of Plant-Beneficial Methylobacteria.
- Author
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Sánchez-López, Ariadna S., Pintelon, Isabel, Stevens, Vincent, Imperato, Valeria, Timmermans, Jean-Pierre, González-Chávez, Carmen, Carrillo-González, Rogelio, Van Hamme, Jonathan, Vangronsveld, Jaco, and Thijs, Sofie
- Subjects
CROTALARIA ,HEAVY-metal tolerant plants ,METHYLOBACTERIUM ,SOIL pollution ,SOIL composition ,HEAVY metals - Abstract
Metal contaminated soils are increasing worldwide. Metal-tolerant plants growing on metalliferous soils are fascinating genetic and microbial resources. Seeds can vertically transmit endophytic microorganisms that can assist next generations to cope with environmental stresses, through yet poorly understood mechanisms. The aims of this study were to identify the core seed endophyte microbiome of the pioneer metallophyte Crotalaria pumila throughout three generations, and to better understand the plant colonisation of the seed endophyte Methylobacterium sp. Cp3. Strain Cp3 was detected in C. pumila seeds across three successive generations and showed the most dominant community member. When inoculated in the soil at the time of flowering, strain Cp3 migrated from soil to seeds. Using confocal microscopy, Cp3-mCherry was demonstrated to colonise the root cortex cells and xylem vessels of the stem under metal stress. Moreover, strain Cp3 showed genetic and in planta potential to promote seed germination and seedling development. We revealed, for the first time, that the seed microbiome of a pioneer plant growing in its natural environment, and the colonisation behaviour of an important plant growth promoting systemic seed endophyte. Future characterization of seed microbiota will lead to a better understanding of their functional contribution and the potential use for seed-fortification applications. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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12. A novel, highly conserved metallothionein family in basidiomycete fungi and characterization of two representative SlMTa and SlMTb genes in the ectomycorrhizal fungus Suillus luteus.
- Author
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Nguyen, Hoai, Rineau, François, Vangronsveld, Jaco, Cuypers, Ann, Colpaert, Jan V., and Ruytinx, Joske
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METALLOTHIONEIN genetics ,BASIDIOMYCETES ,HEAVY metals ,SOIL composition ,ECTOMYCORRHIZAL fungi ,CYSTEINE - Abstract
The basidiomycete Suillus luteus is an important member of the ectomycorrhizal community that thrives in heavy metal polluted soils covered with pioneer pine forests. This study aimed to identify potential heavy metal chelators in S. luteus. Two metallothionein (MT) coding genes, SlMTa and SlMTb, were identified. When heterologously expressed in yeast, both SlMTa and SlMTb can rescue the Cu sensitive mutant from Cu toxicity. In S. luteus, transcription of both SlMTa and SlMTb is induced by Cu but not Cd or Zn. Several putative Cu-sensing and metal-response elements are present in the promoter sequences. These results indicate that SlMTa and SlMTb function as Cu-thioneins. Homologs of the S. luteus MTs are present in 49 species belonging to 10 different orders of the subphylum Agaricomycotina and are remarkably conserved. The length of the proteins, number and distribution of cysteine residues indicate a novel family of fungal MTs. The ubiquitous and highly conserved features of these MTs suggest that they are important for basic cellular functions in species in the subphylum Agaricomycotina. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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13. Olive mill waste biochar: a promising soil amendment for metal immobilization in contaminated soils.
- Author
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Hmid, Amine, Al Chami, Ziad, Sillen, Wouter, De Vocht, Alain, and Vangronsveld, Jaco
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BIOCHAR ,SOIL amendments ,SOIL pollution ,KIDNEY bean ,EISENIA foetida - Abstract
The potential use of biochar from olive mill waste for in situ remediation of metal contaminated soils was evaluated. Biochar was mixed with metal contaminated soil originating from the vicinity of an old zinc smelter. Soil-biochar mixtures were equilibrated for 30 and 90 days. At these time points, Ca(NO) exchangeable metals were determined, and effects of the biochar amendment on soil toxicity were investigated using plants, bacteria, and earthworms. Bean ( Phaseolus vulgaris) growth, metal content, antioxidative enzymes activities, and soluble protein contents were determined. Furthermore, effects on soil microbial communities (activity, diversity, richness) were examined using Biolog ECOplates. After 120 days of soil-biochar equilibration, effects on weight and reproduction of Eisenia foetida were evaluated. With increasing biochar application rate and equilibration period, Ca(NO) exchangeable metals decreased, and growth of bean plants improved; leaf metal contents reduced, the activities of antioxidative stress enzymes decreased, and soluble protein contents increased. Soil microbial activity, richness, and diversity were augmented. Earthworm mortality lowered, and their growth and reproduction showed increasing trends. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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14. Field Evaluation of Willow Under Short Rotation Coppice for Phytomanagement of Metal-Polluted Agricultural Soils.
- Author
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Van Slycken, Stijn, Witters, Nele, Meiresonne, Linda, Meers, Erik, Ruttens, Ann, Van Peteghem, Pierre, Weyens, Nele, Tack, FilipM.G., and Vangronsveld, Jaco
- Subjects
EXPERIMENTAL agriculture ,WILLOWS ,SHORT rotation forestry ,COPPICE forests ,PHYTOREMEDIATION ,SOIL composition ,HEAVY metals ,PLANT extracts - Abstract
Short rotation coppice (SRC) of willow and poplar might be a promising phytoremediation option since it uses fast growing, high biomass producing tree species with often a sufficient metal uptake. This study evaluates growth, metal uptake and extraction potentials of eight willow clones (Belders, Belgisch Rood, Christina, Inger, Jorr, Loden, Tora and Zwarte Driebast) on a metal-contaminated agricultural soil, with total cadmium (Cd) and zinc (Zn) concentrations of 6.5 ± 0.8 and 377 ± 69 mg kg?1soil, respectively. Although, during the first cycle, on average generally low productivity levels (3.7 ton DM (dry matter) ha?1y?1) were obtained on this sandy soil, certain clones exhibited quite acceptable productivity levels (e.g. Zwarte Driebast 12.5 ton DM ha?1y?1). Even at low biomass productivity levels, SRC of willow showed promising removal potentials of 72 g Cd and 2.0 kg Zn ha?1y?1, which is much higher than e.g. energy maize or rapeseed grown on the same soil. Cd and Zn removal can be increased by 40% if leaves are harvested as well. Nevertheless, nowadays the wood price remains the most critical factor in order to implement SRC as an acceptable, economically feasible alternative crop on metal-contaminated agricultural soils. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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15. The role of the kinase OXI1 in cadmium- and copper-induced molecular responses in Arabidopsis thaliana.
- Author
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SMEETS, KAREN, OPDENAKKER, KELLY, REMANS, TONY, FORZANI, CELINE, HIRT, HERIBERT, VANGRONSVELD, JACO, and CUYPERS, ANN
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MITOGEN-activated protein kinases ,EFFECT of cadmium on plants ,SUPEROXIDE dismutase ,OXYLIPINS ,EFFECT of copper on plants ,LIPOXYGENASES ,PLANT defenses - Abstract
The hypothesis that mitogen-activated protein kinase ( MAPK) signalling is important in plant defences against metal stress has become accepted in recent years. To test the role of oxidative signal-inducible kinase ( OXI1) in metal-induced oxidative signalling, the responses of oxi1 knockout lines to environmentally realistic cadmium ( Cd) and copper ( Cu) concentrations were compared with those of wild-type plants. A relationship between OXI1 and the activation of lipoxygenases and other initiators of oxylipin production was observed under these stress conditions, suggesting that lipoxygenase-1 may be a downstream component of OXI1 signalling. Metal-specific differences in OXI1 action were observed. For example, OXI1 was required for the up-regulation of antioxidative defences such as catalase in leaves and Fe-superoxide dismutase in roots, following exposure to Cu, processes that may involve the MEKK1- MKK2- WRKY25 cascade. Moreover, the induction of Cu/Zn superoxide dismutases in Cu-exposed leaves was regulated by OXI1 in a manner that involves fluctuations in the expression of miRNA398. These observations contrast markedly with the responses to Cd exposure, which also involves OXI1-independent pathways but rather involves changes in components mediating intracellular communication. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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16. Metal-Induced Oxidative Stress and Plant Mitochondria.
- Author
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Keunen, Els, Remans, Tony, Bohler, Sacha, Vangronsveld, Jaco, and Cuypers, Ann
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OXIDATIVE stress ,PLANT mitochondria ,ECOLOGY ,ELECTRON transport ,PLANT adaptation ,HEAVY metals - Abstract
A general status of oxidative stress in plants caused by exposure to elevated metal concentrations in the environment coincides with a constraint on mitochondrial electron transport, which enhances ROS accumulation at the mitochondrial level. As mitochondria are suggested to be involved in redox signaling under environmental stress conditions, mitochondrial ROS can initiate a signaling cascade mediating the overall stress response, i.e., damage versus adaptation. This review highlights our current understanding of metal-induced responses in plants, with focus on the production and detoxification of mitochondrial ROS. In addition, the potential involvement of retrograde signaling in these processes will be discussed. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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17. Endophytes and Their Potential to Deal with Co-Contamination of Organic Contaminants (Toluene) and Toxic Metals (Nickel) During Phytoremediation.
- Author
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Weyens, Nele, Truyens, Sascha, Saenen, Eline, Boulet, Jana, Dupae, Joke, Taghavi, Safiyh, van der Lelie, Daniel, Carleer, Robert, and Vangronsveld, Jaco
- Subjects
ENDOPHYTES ,TOLUENE ,HEAVY metals ,PHYTOREMEDIATION ,LUPINUS luteus ,BURKHOLDERIA ,PHYTOTOXICITY - Abstract
The aim was to investigate if engineered endophytes that are capable of degrading organic contaminants, and deal with or ideally improve uptake and translocation of toxic metals, can improve phytoremediation of mixed organic-metal pollution. As a model system, yellow lupine was inoculated with the endophyte Burkholderia cepacia VM1468 possessing (a) the pTOM-Bu61 plasmid, coding for constitutive toluene/TCE degradation, and (b) the chromosomally inserted ncc-nre Ni resistance/sequestration system. As controls, plants were inoculated with B. vietnamiensis BU61 (pTOM-Bu61) and B. cepacia BU72 (containing the ncc-nre Ni resistance/sequestration system). Plants were exposed to mixes of toluene and Ni. Only inoculation with B. cepacia VM1468 resulted in decreased Ni and toluene phytotoxicity, as measured by a protective effect on plant growth and decreased activities of enzymes involved in antioxidative defence (catalase, guaiacol peroxidase, superoxide dismutase) in the roots. Besides, plants inoculated with B. cepacia VM1468 and B. vietnamiensis BU61 released less toluene through the leaves than non-inoculated plants and those inoculated with B. cepacia BU72. Ni-uptake in roots was slightly increased for B. cepacia BU72 inoculated plants. These results indicate that engineered endophytes have the potential to assist their host plant to deal with co-contamination of toxic metals and organic contaminants during phytoremediation. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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18. Cd-tolerant Suillus luteus: A fungal insurance for pines exposed to Cd
- Author
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Krznaric, Erik, Verbruggen, Nathalie, Wevers, Jan H.L., Carleer, Robert, Vangronsveld, Jaco, and Colpaert, Jan V.
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CADMIUM ,SOIL composition ,HEAVY metals ,PINE ,BIOLOGICAL adaptation ,SCOTS pine ,SYMBIOSIS ,ECTOMYCORRHIZAL fungi ,BIOMASS ,PLANT protection - Abstract
Soil metal pollution can trigger evolutionary adaptation in soil-borne organisms. An in vitro screening test showed cadmium adaptation in populations of Suillus luteus (L.: Fr.) Roussel, an ectomycorrhizal fungus of pine trees. Cadmium stress was subsequently investigated in Scots pine (Pinus sylvestris L.) seedlings inoculated with a Cd-tolerant S. luteus, isolated from a heavy metal contaminated site, and compared to plants inoculated with a Cd-sensitive isolate from a non-polluted area. A dose–response experiment with mycorrhizal pines showed better plant protection by a Cd-adapted fungus: more fungal biomass and a higher nutrient uptake at high Cd exposure. In addition, less Cd was transferred to aboveground plant parts. Because of the key role of the ectomycorrhizal symbiosis for tree fitness, the evolution of Cd tolerance in an ectomycorrhizal partner such as S. luteus can be of major importance for the establishment of pine forests on Cd-contaminated soils. [Copyright &y& Elsevier]
- Published
- 2009
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19. PHYTOREMEDIATION FOR HEAVY METAL-CONTAMINATED SOILS COMBINED WITH BIOENERGY PRODUCTION.
- Author
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Van Ginneken, Luc, Meers, Erik, Guisson, Ruben, Ruttens, Ann, Elst, Kathy, Tack, Filip M. G., Vangronsveld, Jaco, Diels, Ludo, and Dejonghe, Winnie
- Subjects
PHYTOREMEDIATION ,SOIL remediation ,SOIL pollution ,HEAVY metals ,BIOAVAILABILITY ,BIODEGRADATION ,BIOMASS energy - Abstract
Copyright of Journal of Environmental Engineering & Landscape Management is the property of Vilnius Gediminas Technical University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2007
20. Endophytic bacterial diversity in poplar trees growing on a BTEX-contaminated site: The characterisation of isolates with potential to enhance phytoremediation.
- Author
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Moore, Fiona Porteous, Barac, Tanja, Borremans, Brigitte, Oeyen, Licy, Vangronsveld, Jaco, van der Lelie, Daniel, Campbell, Colin D., and Moore, Edward R.B.
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BACTERIA ,PHYTOREMEDIATION ,GENES ,POLLUTANTS ,HEAVY metals ,ANTIBIOTICS - Abstract
Abstract: The diversity of endophytic bacteria found in association with poplar was investigated as part of a larger study to assess the possibility and practicality of using endophytic bacteria to enhance in situ phytoremediation. Endophytic bacteria were isolated from the root, stem and leaf of two cultivars of poplar tree growing on a site contaminated with BTEX compounds. They were further characterised genotypically by comparative sequence analysis of partial 16S rRNA genes and BOX-PCR genomic DNA fingerprinting, and phenotypically by their tolerance to a range of target pollutants, heavy metals and antibiotics. One hundred and 21 stable, morphologically distinct isolates were obtained, belonging to 21 genera, although six isolates could not be identified with confidence to a genus. The endophytic bacteria exhibited marked spatial compartmentalisation within the plant, suggesting there are likely to be species-specific and non-specific associations between bacteria and plants. A number of isolates demonstrated the ability to degrade BTEX compounds or to grow in the presence of TCE. This study demonstrates that within the diverse bacterial communities found in poplar several endophytic strains are present that have the potential to enhance phytoremediation strategies. [Copyright &y& Elsevier]
- Published
- 2006
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21. Cadmium Accumulation and Tolerance of Two Salix Genotypes Hydroponically Grown in Presence of Cadmium.
- Author
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Vassilev, Andon, Perez-Sanz, Araceli, Semane, Brahim, Carleer, Robert, and Vangronsveld, Jaco
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SALIX alba ,WILLOWS ,SALIX viminalis ,ENZYMES ,HEAVY metals ,GENETIC polymorphisms ,CADMIUM ,BIOMASS ,PLANT biomass - Abstract
Two Salix genotypes ( Salix alba , clone LUC-31—genotype I and Salix viminalis , clone STOTT—genotype II) were grown hydroponically for 14 d at increasing concentrations of cadmium (Cd) (0, 5, 10, and 15 μM) in order to study both Cd accumulation and genotype tolerance. Growth responses, photosynthetic performance, activities of metal-responsive enzymes, and changes in some nutrients content were used as evaluation criteria. The Genotype II assimilated more Cd (maximum leaf concentration 47.8± 2.9 mg Cd kg -1 DW) compared with the Genotype I and, based on both morphological (root length, total biomass) and metabolic indicators (photosynthetic performance, activities of metal-responsive enzymes, changes in several nutrients), was classified as less tolerant to this heavy metal. However, it is considered to be more suitable for Cd phytoextraction, as it would compensate for its somewhat lower tolerance with its higher Cd accumulation, as well as its higher productivity under field conditions. [ABSTRACT FROM AUTHOR]
- Published
- 2005
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22. Exopolysaccharide Carbohydrate Structure and Biofilm Formation by Rhizobium leguminosarum bv. trifolii Strains Inhabiting Nodules of Trifolium repens Growing on an Old Zn–Pb–Cd-Polluted Waste Heap Area.
- Author
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Oleńska, Ewa, Małek, Wanda, Kotowska, Urszula, Wydrych, Jerzy, Polińska, Weronika, Swiecicka, Izabela, Thijs, Sofie, Vangronsveld, Jaco, and Corsaro, Maria Michela
- Subjects
MICROBIAL exopolysaccharides ,RHIZOBIUM leguminosarum ,WHITE clover ,BACTERIAL cell walls ,BIOFILMS ,HEAVY metals ,PHYTOCHELATINS - Abstract
Heavy metals polluting the 100-year-old waste heap in Bolesław (Poland) are acting as a natural selection factor and may contribute to adaptations of organisms living in this area, including Trifolium repens and its root nodule microsymbionts—rhizobia. Exopolysaccharides (EPS), exuded extracellularly and associated with bacterial cell walls, possess variable structures depending on environmental conditions; they can bind metals and are involved in biofilm formation. In order to examine the effects of long-term exposure to metal pollution on EPS structure and biofilm formation of rhizobia, Rhizobium leguminosarum bv. trifolii strains originating from the waste heap area and a non-polluted reference site were investigated for the characteristics of the sugar fraction of their EPS using gas chromatography mass-spectrometry and also for biofilm formation and structural characteristics using confocal laser scanning microscopy under control conditions as well as when exposed to toxic concentrations of zinc, lead, and cadmium. Significant differences in EPS structure, biofilm thickness, and ratio of living/dead bacteria in the biofilm were found between strains originating from the waste heap and from the reference site, both without exposure to metals and under metal exposure. Received results indicate that studied rhizobia can be assumed as potentially useful in remediation processes. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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23. Long-Term Cd Exposure Alters the Metabolite Profile in Stem Tissue of Medicago sativa.
- Author
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Gutsch, Annelie, Hendrix, Sophie, Guerriero, Gea, Renaut, Jenny, Lutts, Stanley, Alseekh, Saleh, Fernie, Alisdair R., Hausman, Jean-Francois, Vangronsveld, Jaco, Cuypers, Ann, and Sergeant, Kjell
- Subjects
ALFALFA ,METABOLITES ,METALLOTHIONEIN ,HEAVY metals ,ENVIRONMENTAL sciences ,PLANT cells & tissues ,ANTHROPOGENIC soils ,PROLINE - Abstract
As a common pollutant, cadmium (Cd) is one of the most toxic heavy metals accumulating in agricultural soils through anthropogenic activities. The uptake of Cd by plants is the main entry route into the human food chain, whilst in plants it elicits oxidative stress by unbalancing the cellular redox status. Medicago sativa was subjected to chronic Cd stress for five months. Targeted and untargeted metabolic analyses were performed. Long-term Cd exposure altered the amino acid composition with levels of asparagine, histidine and proline decreasing in stems but increasing in leaves. This suggests tissue-specific metabolic stress responses, which are often not considered in environmental studies focused on leaves. In stem tissue, profiles of secondary metabolites were clearly separated between control and Cd-exposed plants. Fifty-one secondary metabolites were identified that changed significantly upon Cd exposure, of which the majority are (iso)flavonoid conjugates. Cadmium exposure stimulated the phenylpropanoid pathway that led to the accumulation of secondary metabolites in stems rather than cell wall lignification. Those metabolites are antioxidants mitigating oxidative stress and preventing cellular damage. By an adequate adjustment of its metabolic composition, M. sativa reaches a new steady state, which enables the plant to acclimate under chronic Cd stress. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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24. Identifying the Pressure Points of Acute Cadmium Stress Prior to Acclimation in Arabidopsis thaliana.
- Author
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Deckers, Jana, Hendrix, Sophie, Prinsen, Els, Vangronsveld, Jaco, and Cuypers, Ann
- Subjects
CADMIUM ,HEAVY metals ,HYDROGEN peroxide ,POLLUTANTS ,ACCLIMATIZATION ,PRESSURE - Abstract
The toxic metal cadmium (Cd) is a major soil pollutant. Knowledge on the acute Cd-induced stress response is required to better understand the triggers and sequence of events that precede plant acclimation. Therefore, we aimed to identify the pressure points of Cd stress using a short-term exposure set-up ranging from 0 h to 24 h. Acute responses related to glutathione (GSH), hydrogen peroxide (H
2 O2 ), 1-aminocyclopropane-1-carboxylic acid (ACC), ethylene and the oxidative challenge were studied at metabolite and/or transcript level in roots and leaves of Arabidopsis thaliana either exposed or not to 5 µM Cd. Cadmium rapidly induced root GSH depletion, which might serve as an alert response and modulator of H2 O2 signalling. Concomitantly, a stimulation of root ACC levels was observed. Leaf responses were delayed and did not involve GSH depletion. After 24 h, a defined oxidative challenge became apparent, which was most pronounced in the leaves and concerted with a strong induction of leaf ACC synthesis. We suggest that root GSH depletion is required for a proper alert response rather than being a merely adverse effect. Furthermore, we propose that roots serve as command centre via a.o. root-derived ACC/ethylene to engage the leaves in a proper stress response. [ABSTRACT FROM AUTHOR]- Published
- 2020
- Full Text
- View/download PDF
25. Enhancing the Rice Seedlings Growth Promotion Abilities of Azoarcus sp. CIB by Heterologous Expression of ACC Deaminase to Improve Performance of Plants Exposed to Cadmium Stress.
- Author
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Fernández-Llamosas, Helga, Ibero, Juan, Thijs, Sofie, Imperato, Valeria, Vangronsveld, Jaco, Díaz, Eduardo, and Carmona, Manuel
- Subjects
PLANT growth ,PLANT performance ,REACTIVE oxygen species ,POLLUTANTS ,CADMIUM ,SEEDLINGS - Abstract
Environmental pollutants can generate stress in plants causing increased ethylene production that leads to the inhibition of plant growth. Ethylene production by the stressed plant may be lowered by Plant Growth-Promoting Bacteria (PGPB) that metabolizes the immediate precursor of ethylene 1-aminocyclopropane-1-carboxylate (ACC). Thus, engineering PGPB with ACC deaminase activity can be a promising alternative to mitigate the harmful effects of pollutants and thus enhance plant production. Here we show that the aromatics-degrading and metal-resistant Azoarcus sp. CIB behaves as a PGP-bacterium when colonizing rice as an endophyte, showing a 30% increment in plant weight compared to non-inoculated plants. The cloning and expression of an acdS gene led to a recombinant strain, Azoarcus sp. CIB (pSEVA237acdS), possessing significant ACC deaminase activity (6716 nmol mg
−1 h−1 ), constituting the first PGPB of the Rhodocyclaceae family equipped with this PGP trait. The recombinant CIB strain acquired the ability to protect inoculated rice plants from the stress induced by cadmium (Cd) exposure and to increase the Cd concentration in rice seedlings. The observed decrease of the levels of reactive oxygen species levels in rice roots confirms such a protective effect. The broad-host-range pSEVA237acdS plasmid paves the way to engineer PGPB with ACC deaminase activity to improve the growth of plants that might face stress conditions. [ABSTRACT FROM AUTHOR]- Published
- 2020
- Full Text
- View/download PDF
26. Trifolium repens-Associated Bacteria as a Potential Tool to Facilitate Phytostabilization of Zinc and Lead Polluted Waste Heaps.
- Author
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Oleńska, Ewa, Imperato, Valeria, Małek, Wanda, Włostowski, Tadeusz, Wójcik, Małgorzata, Swiecicka, Izabela, Vangronsveld, Jaco, and Thijs, Sofie
- Subjects
MINE waste ,PHYTOREMEDIATION ,METAL wastes ,CLOVER ,WHITE clover ,HEAVY metals ,LEAD - Abstract
Heavy metals in soil, as selective agents, can change the structure of plant-associated bacterial communities and their metabolic properties, leading to the selection of the most-adapted strains, which might be useful in phytoremediation. Trifolium repens, a heavy metal excluder, naturally occurs on metal mine waste heaps in southern Poland characterized by high total metal concentrations. The purpose of the present study was to assess the effects of toxic metals on the diversity and metabolic properties of the microbial communities in rhizospheric soil and vegetative tissues of T. repens growing on three 70–100-years old Zn–Pb mine waste heaps in comparison to Trifolium-associated bacteria from a non-polluted reference site. In total, 113 cultivable strains were isolated and used for 16S rRNA gene Sanger sequencing in order to determine their genetic affiliation and for in vitro testing of their plant growth promotion traits. Taxa richness and phenotypic diversity in communities of metalliferous origin were significantly lower (p < 0.0001) compared to those from the reference site. Two strains, Bacillus megaterium BolR EW3_A03 and Stenotrophomonas maltophilia BolN EW3_B03, isolated from a Zn–Pb mine waste heap which tested positive for all examined plant growth promoting traits and which showed co-tolerance to Zn, Cu, Cd, and Pb can be considered as potential facilitators of phytostabilization. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
27. A role for plant-associated bacteria in metal mobilization and uptake by plants and phytoextraction?
- Author
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Vangronsveld, Jaco, Croes, Sarah, Janssen, Jolien, Truyens, Sascha, Thijs, Sofie, and Weyens, Nele
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- *
HYPERACCUMULATOR plants , *HEAVY metals , *SOIL composition , *SOIL remediation , *RHIZOSPHERE , *EFFECT of metals on plants , *PHYTOREMEDIATION - Abstract
The article discusses bacteria associated with hyperaccumulator flora, their potential to clean up soil contaminated by metals and the various bacteria types. It also seeks to examine their ability to enhance trance elements in the rhizosphere and aid plants in their uptake, aid plants in developing metal tolerance, and to improve phytoextraction efficiency. It notes that research data of plants immunized by bacteria is inconclusive with regard to phytoremediation efficiency.
- Published
- 2014
28. SOIL-SOLUTION SPECIATION OF Cd AS AFFECTED BY SOIL CHARACTERISTICS IN UNPOLLUTED AND POLLUTED SOILS.
- Author
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Meers, Erik, Unamuno, Virginia, Vandegehuchte, Michiel, Vanbroekhoven, Karolien, Geebelen, Wouter, Samson, Roeland, Vangronsveld, Jaco, Diels, Ludo, Ruttens, Ann, Du Laing, Gijs, and Tack, Filip
- Subjects
SOIL composition ,METALS ,HEAVY metals ,NITRATES ,CARBONATES - Abstract
Total metal content by itself is insufficient as a measure to indicate actual environmental risk. Understanding the mobility of heavy metals in the soil and their speciation in the soil solution is of great importance for accurately assessing environmental risks posed by these metals. In a first explorative study, the effects of general soil characteristics on Cd mobility were evaluated and expressed in the form of empirical formulations. The most important factors influencing mobility of Cd proved to be pH and total soil content. This may indicate that current legislation expressing the requirement for soil sanitation in Flanders (Belgium) as a function of total soil content, organic matter, and clay does not successfully reflect actual risks. Current legal frameworks focusing on total content, therefore, should be amended with criteria that are indicative of metal mobility and availability and are based on physicochemical soil properties. In addition, soil-solution speciation was performed using two independent software packages (Visual Minteq 2.23 and Windermere Humic Aqueous model VI [WHAM VI]). Both programs largely were in agreement in concern to Cd speciation in all 29 soils under study. Depending on soil type, free ion and the organically complexed forms were the most abundant species. Additional inorganic soluble species were sulfates and chlorides. Minor species in solution were in the form of nitrates, hydroxides, and carbonates, the relative importance of which was deemed insignificant in comparison to the four major species. [ABSTRACT FROM AUTHOR]
- Published
- 2005
- Full Text
- View/download PDF
29. The influence of EDDS on the metabolic and transcriptional responses induced by copper in hydroponically grown Brassica carinata seedlings
- Author
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Cestone, Benedetta, Cuypers, Ann, Vangronsveld, Jaco, Sgherri, Cristina, and Navari-Izzo, Flavia
- Subjects
- *
TRANSCRIPTION factors , *HYDROPONICS , *BRASSICA , *PLANT growth , *SEEDLINGS , *HEAVY metals , *SOIL pollution - Abstract
Abstract: To improve the knowledge about the use of plants for the removal of toxic metals from contaminated soils, metabolic and transcriptional responses of Brassica carinata to different forms of copper (Cu) were studied. Two-week-old hydroponically grown seedlings were exposed for 24h to 30μM CuSO4 or CuEDDS. CuSO4 appeared to be more toxic than CuEDDS as roots showed higher levels of thiobarbituric acid reactive substances (TBARS) and increased relative leakage ratios (RLR), although the superoxide dismutase (SOD, EC 1.15.1.1) activity increased following both exposures. In CuSO4-exposed seedlings the higher toxicity was underlined by increased transcription of lipoxygenases (EC 1.13.11.12) and NADPH oxidases (EC 1.6.99.6) and by the higher Cu accumulation in both tissues compared to CuEDDS exposure. The presence of EDDS increased Cu translocation, which resulted 5-times higher than when exposed to CuSO4. Decreases in catalase (CAT, EC 1.11.1.6), ascorbate peroxidase (APX, EC 1.11.1.11) and glutathione reductase (GR, EC 1.6.4.2) activities together with increases of reduced glutathione (GSH) and tocopherols and a reduction of lipoic acid (LA) were observed in roots of CuSO4-exposed seedlings. On the contrary, CuEDDS exposure induced a general increase in enzyme activities and decreases in ascorbate (AsA) and tocopherol levels. In the primary leaves, in both exposures Cu differently affected the oxidative stress responses indicating that the cellular redox balance was anyway maintained. EDDS plays a crucial role in B. carinata tolerance to oxidative stress induced by Cu and might be proposed to improve the efficiency of Cu phytoextraction. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
- View/download PDF
30. Unraveling uranium induced oxidative stress related responses in Arabidopsis thaliana seedlings. Part II: responses in the leaves and general conclusions
- Author
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Vanhoudt, Nathalie, Cuypers, Ann, Horemans, Nele, Remans, Tony, Opdenakker, Kelly, Smeets, Karen, Bello, Daniel Martinez, Havaux, Michel, Wannijn, Jean, Van Hees, May, Vangronsveld, Jaco, and Vandenhove, Hildegarde
- Subjects
- *
URANIUM , *OXIDATIVE stress , *ARABIDOPSIS thaliana , *SEEDLINGS , *HEAVY metals , *GENE expression , *LIPOXYGENASES , *SUPEROXIDE dismutase , *LEAF physiology - Abstract
Abstract: The cellular redox balance seems an important modulator under heavy metal stress. While for other heavy metals these processes are well studied, oxidative stress related responses are also known to be triggered under uranium stress but information remains limited. This study aimed to further unravel the mechanisms by which plants respond to uranium stress. Seventeen-day-old Arabidopsis thaliana seedlings, grown on a modified Hoagland solution under controlled conditions, were exposed to 0, 0.1, 1, 10 and 100 μM uranium for 1, 3 and 7 days. While in Part I of this study oxidative stress related responses in the roots were discussed, this second Part II discusses oxidative stress related responses in the leaves and general conclusions drawn from the results of the roots and the leaves will be presented. As several responses were already visible following 1 day exposure, when uranium concentrations in the leaves were negligible, a root-to-shoot signaling system was suggested in which plastids could be important sensing sites. While lipid peroxidation, based on the amount of thiobarbituric acid reactive compounds, was observed after exposure to 100 μM uranium, affecting membrane structure and function, a transient concentration dependent response pattern was visible for lipoxygenase initiated lipid peroxidation. This transient character of uranium stress responses in leaves was emphasized by results of lipoxygenase (LOX2) and antioxidative enzyme transcript levels, enzyme capacities and glutathione concentrations both in time as with concentration. The ascorbate redox balance seemed an important modulator of uranium stress responses in the leaves as in addition to the previous transient responses, the total ascorbate concentration and ascorbate/dehydroascorbate redox balance increased in a concentration and time dependent manner. This could represent either a slow transient response or a stable increase with regard to plant acclimation to uranium stress. [Copyright &y& Elsevier]
- Published
- 2011
- Full Text
- View/download PDF
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