Search

Your search keyword '"Dattilo D"' showing total 2 results
Start Over Author "Dattilo D" Topic heat-shock proteins
2 results on '"Dattilo D"'

1. Helicobacter pylori heat shock protein B (HspB) localizes in vivo in the gastric mucosa and MALT lymphoma.

Author

De Luca A, De Falco M, Manente L, Dattilo D, Lucariello A, Esposito V, Gnarini M, Citro G, Baldi A, Tufano MA, and Iaquinto G

Subjects
Animals, Antigens, Bacterial genetics, Antigens, Bacterial metabolism, Bacterial Proteins genetics, Bacterial Vaccines metabolism, Biopsy, Cell Line, Gastric Mucosa cytology, Gastric Mucosa microbiology, Gastric Mucosa pathology, Heat-Shock Proteins genetics, Helicobacter Infections metabolism, Helicobacter Infections pathology, Humans, Immunohistochemistry, Lymphoma, B-Cell, Marginal Zone pathology, Rabbits, Bacterial Proteins metabolism, Gastric Mucosa metabolism, Heat-Shock Proteins metabolism, Helicobacter pylori metabolism, Lymphoma, B-Cell, Marginal Zone metabolism
Abstract

Heat shock protein B (HspB) is one of the dominant proteins recognized by most Helicobacter pylori-infected persons and is being considered as potential candidates for subunit vaccines. In the present study we describe the generation of an antibody against HspB and its use in immunohistochemical assays on gastric biopsies. We have demonstrated that our rabbit polyclonal antibody against HspB did not recognize any protein in lysates from a lung human epithelial cell (H1299) line and did not cross-react with the other members of human heat shock proteins. Secondly, we have observed that in gastric biopsies, HspB immunostaining was present inside the cytoplasm of human epithelial cells with a particular localization in the apical portion of gastric epithelial cells other than in the extracellular spaces among gastric cells of human stomach. Finally, we have demonstrated a cytoplasmic HspB immunostaining in groups of neoplastic cells of MALT lymphoma. In conclusion, our observations suggest a possible involvement of HspB in the pathogenesis of H. pylori-related pathologies such as gastritis, ulcer and gastric cancer., ((c) 2008 Wiley-Liss, Inc.)

Published
2008
Full Text
View/download PDF

2. Helicobacter pylori heat shock protein B (HspB) localizes in vivo in the gastric mucosa and MALT lymphoma

Author

Maria De Falco, Mariarosaria Gnarini, Doriana Dattilo, Vincenzo Esposito, Antonio De Luca, Maria Antonietta Tufano, Angela Lucariello, Gaetano Iaquinto, Lucrezia Manente, Gennaro Citro, Alfonso Baldi, DE LUCA, A, DE FALCO, Maria, Manente, L, Dattilo, D, Lucariello, A, Esposito, V, Gnarini, M, Citro, G, Baldi, A, Tufano, Ma, Iaquinto, G., DE LUCA, Antonio, DE FALCO, M, and Baldi, Alfonso

Subjects
Lymphoma, Physiology, Biopsy, Clinical Biochemistry, Marginal Zone, Biology, Cell Line, Helicobacter Infections, Antigen, Bacterial Proteins, Heat shock protein, medicine, Gastric mucosa, Animals, Humans, Antigens, Bacterial, Bacterial Vaccines, Gastric Mucosa, Heat-Shock Proteins, Helicobacter pylori, Immunohistochemistry, Lymphoma, B-Cell, Marginal Zone, Rabbits, Antigens, B cell, Bacterial, B-Cell, MALT lymphoma, Cell Biology, biology.organism_classification, medicine.disease, Molecular biology, medicine.anatomical_structure, Immunology, biology.protein, Antibody, Immunostaining
Abstract

none 11 Heat shock protein B (HspB) is one of the dominant proteins recognized by most Helicobacter pylori-infected persons and is being considered as potential candidates for subunit vaccines. In the present study we describe the generation of an antibody against HspB and its use in immunohistochemical assays on gastric biopsies. We have demonstrated that our rabbit polyclonal antibody against HspB did not recognize any protein in lysates from a lung human epithelial cell (H1299) line and did not cross-react with the other members of human heat shock proteins. Secondly, we have observed that in gastric biopsies, HspB immunostaining was present inside the cytoplasm of human epithelial cells with a particular localization in the apical portion of gastric epithelial cells other than in the extracellular spaces among gastric cells of human stomach. Finally, we have demonstrated a cytoplasmic HspB immunostaining in groups of neoplastic cells of MALT lymphoma. In conclusion, our observations suggest a possible involvement of HspB in the pathogenesis of H. pylori-related pathologies such as gastritis, ulcer and gastric cancer. © 2008 Wiley-Liss, Inc. mixed DE LUCA A; DE FALCO M; MANENTE L; DATTILO D; LUCARIELLO A; ESPOSITO V; GNARINI M; CITRO G; BALDI A; TUFANO MA; IAQUINTO G De Luca A.; De Falco M.; Manente L.; Dattilo D.; Lucariello A.; Esposito V.; Gnarini M.; Citro G.; Baldi A.; Tufano M.A.; Iaquinto G.

Published
2008

Catalog

Books, media, physical & digital resources
See catalog results