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Background and aims: Given the worldwide effort to improve the nitrogen (N) economy of crops, it is critical to understand the mechanisms of improved N uptake which have resulted from selection pressure for grain yield in Australian wheat ( Triticum aestivum L.). Changes in root system traits and N uptake were examined in nine Australian wheat varieties released between 1958 and 2007. Methods: Wheat varieties were grown in rhizo-boxes in a glasshouse. We measured nitrogen uptake and mapped root growth and proliferation to quantify root length density (RLD), root length per plant, root biomass, specific root length, and plant nitrogen uptake per unit root length. Results: Selection for yield reduced total RLD and total root length, and increased N uptake per unit root length that overrode the reduction in root system size, effectively explaining the increase in N uptake. Importantly, N uptake in our experiment under controlled conditions matched field measurements, reinforcing the agronomic significance of the present study. Conclusions: Wheat varieties released in Australia between 1958 and 2007 increased their N uptake, not because of increasing their root length and RLD, but for progressively increasing the efficiency of their root system in capturing N. Our collection of varieties is therefore an interesting model to probe for variation in the affinity of the root system for nitrate. [ABSTRACT FROM AUTHOR]

We investigated the use of film-forming antitranspirants (AT) to reduce transpiration and alleviate the adverse effects of late-season drought on wheat ( Triticum aestivum L.) growth and yield. Two experiments were conducted in a controlled-temperature glasshouse from April to November 2014, to compare two watering regimes (well watered and water deficit) and three AT treatments (unsprayed control, sprayed before boot swollen and sprayed before anthesis complete). We measured plant water use, transpiration rate, stomatal conductance and photosynthesis. Relative leaf turgor was measured in real time using a non-destructive method of leaf patch clamp pressure. Drought stress reduced daily water use, transpiration rate, stomatal conductance and leaf turgor in wheat plants after about four days. In contrast, these measurements rapidly declined soon after AT application in both well-watered and water-deficit plants. Nevertheless, once soil moisture deficit increased markedly, AT-treated water-deficit plants maintained significantly higher levels of photosynthesis than untreated plants. Drought stress reduced grain yield in unsprayed control plants by more than 40%, compared to well-watered control plants, mainly due to fewer grains per spike. In contrast, drought stress with AT application prior to the most drought-sensitive boot stage reduced yield by only 14%. These results suggest that AT has the potential to improve wheat yields with late-season drought, as is common in semiarid regions; although, more research is required to test the wider applicability of these results in field conditions. [ABSTRACT FROM AUTHOR]