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For the first time, grain boundary diffusion of a solute is measured in a single grain boundary in the C kinetic regime after common Harrison’s classification. The radiotracer technique is used to determine the diffusion coefficients in Cu Σ5 (310) [001] bicrystal along and perpendicular to the [001] misorientation axis. The anisotropy of grain boundary diffusion increases slightly with decreasing temperature. However, it remains moderate, within a factor of two to three for the temperature interval studied. [Copyright &y& Elsevier]