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Background: Grains make up a large proportion of both human and animal diets. With threats to food production, such as climate change, growing sustainable and successful crops is essential to food security in the future. Germination is one of the most important stages in a plant's lifecycle and is key to the success of the resulting plant as the grain undergoes morphological changes and the development of specific organs. Micro-computed tomography is a non-destructive imaging technique based on the differing x-ray attenuations of materials which we have applied for the accurate analysis of grain morphology during the germination phase. Results: Micro Computed Tomography conditions and parameters were tested to establish an optimal protocol for the 3-dimensional analysis of barley grains. When comparing optimal scanning conditions, it was established that no filter, 0.4 degrees rotation step, 5 average frames, and 2016 × 1344 camera binning is optimal for imaging germinating grains. It was determined that the optimal protocol for scanning during the germination timeline was to scan individual grains at 0 h after imbibition (HAI) and then the same grain again at set time points (1, 3, 6, 24 HAI) to avoid any negative effects from X-ray radiation or disruption to growing conditions. Conclusion: Here we sought to develop a method for the accurate analysis of grain morphology without the negative effects of possible radiation exposure. Several factors have been considered, such as the scanning conditions, reconstruction, and possible effects of X-ray radiation on the growth rate of the grains. The parameters chosen in this study give effective and reliable results for the 3-dimensional analysis of macro structures within barley grains while causing minimal disruption to grain development. [ABSTRACT FROM AUTHOR]

Rationale: Alkylresorcinols (AR) are cereal-specific biomarkers and have recently been found in archaeological pots. However, their low concentrations and high susceptibility to degradation make them difficult to detect using conventional gas chromatography mass spectrometry (GC/MS). Here we describe the development of a more sensitive liquid chromatography mass spectrometry (LC/MS) method to detect these compounds. Method: A method based on the use of ultra-high-performance liquid chromatography (UHPLC) coupled to an Orbitrap mass analyser was established and validated for the detection of low-concentration ARs in pottery. During the preliminary experiments, UHPLC-Q/Orbitrap MS (ultra-high-performance liquid chromatography-quadrupole/Orbitrap mass spectrometry) was demonstrated to be more sensitive, and a wide range of AR homologues in cereal extracts were detected, unlike UHPLC-QTOFMS (ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry) and GC/MS. The developed method was utilised to profile AR homologue distribution in modern cereal samples and reanalyse AR-containing pots from the archaeological site of Must Farm. Results: A highly sensitive LC/MS method with a limit of detection (LOD) of 0.02 µg/g and a limit of quantification (LOQ) of 0.06 µg/g was used to profile ARs in five modern cereal grains. The obtained LOD is 250 times lower than that obtained using the conventional GC/MS approach. AR 21:0 was the most abundant homologue in all four Triticum spp.--einkorn, emmer, Khorasan wheat and common wheat. Meanwhile, AR 25:0 was the predominant homologue in barley, potentially enabling differentiation between wheat and barley. The developed LC/MS-based method was successfully used to analyse ARs extracted from Must Farm potsherds and identified the cereal species most likely processed in the pots--emmer wheat. Conclusion: The described method offers an alternative and more sensitive approach for detecting and identifying ARs in ancient pottery. It has been successfully utilised to detect AR homologues in archaeological samples and discriminate which cereal species--wheat and barley--were processed in the pots. [ABSTRACT FROM AUTHOR]

A properly designed crop rotation contributes to the equilibrium of the agro-ecosystem and the volume and quality of the yield. The cultivation of spelt in crop rotations enriches its biodiversity and provides grains with many different types of nutritional value. The aim of this current study was to investigate how the distribution of winter spelt in different positions and after different forecrops in four-field crop rotations would affect the technological quality of the grain, the nutrient content of the grain, and the grain yield. A 6-year field experiment, designed in a randomised block, was conducted from 2012 to 2018 in north-eastern Poland (53°35′47″ N, 19°51′20″ E). This study provides the results from a 6-year (2013–2018) field experiment. The spelt was cultivated in four crop rotations: CR1—winter rape + catch crop (blue tansy), spring barley, field pea and winter spelt; CR2,—winter rape, winter spelt + catch crop (blue tansy), field pea and winter spelt; CR3—winter rape + catch crop (blue tansy), field pea, winter spelt and winter spelt; and CR4—winter rape, winter spelt + catch crop (blue tansy), spring barley and winter spelt. This study evaluated grain yield and the following grain parameters: the total protein, wet gluten and starch contents, the Zeleny index, the falling number, the weight of 1000 grains, the N, P, K, Mg, Ca, Cu, Fe, Zn and Mn contents, and the grain yield. The results were assessed at the significance level p < 0.05. It was demonstrated that the cultivation of spelt in all four crop rotations after winter rape and after field pea was characterised by higher protein and wet gluten contents, Zeleny index value and falling number, a greater weight of 1000 grains, higher N, P, Fe and Zn contents, and greater grain yield than those harvested from the crop rotations CR3 and CR4 after spelt and after barley. It was demonstrated that the cultivation of spelt in crop rotations CR3 and CR4, in succession after spelt and after barley, caused deterioration in grain quality (lower protein and gluten contents, a lower Zeleny index value, a lower falling number, and a smaller weight of 1000 grains, and the N, P, Fe and Zn contents). In addition, a smaller grain yield was obtained from these crop rotation fields. Regardless of the type of crop rotation, the cultivation of spelt after winter rape and after pea produced a high yield and a good quality yield of this cereal. Due to the lower yield of grain and its lower quality, it is not recommended that winter spelt is grown after each other or after spring barley. [ABSTRACT FROM AUTHOR]

The cuticle covering aerial organs of land plants is well known to protect against desiccation. Cuticles also play diverse and specialized functions, including organ separation, depending on plant and tissue. Barley shows a distinctive cuticular wax bloom enriched in β -diketones on leaf sheaths, stem nodes and internodes and inflorescences. Barley also develops a sticky surface on the outer pericarp layer of its grain fruit leading to strongly adhered hulls, 'covered grain', important for embryo protection and seed dispersal. While the transcription factor-encoding gene HvNUDUM (HvNUD) appears essential for adherent hulls, little is understood about how the pericarp cuticle changes during adhesion or whether changes in pericarp cuticles contribute to another phenotype where hulls partially shed, called 'skinning'. To that end, we screened barley lines for hull adhesion defects, focussing on the Eceriferum (= waxless, cer) mutants. Here, we show that the cer-xd allele causes defective wax blooms and compromised hull adhesion, and results from a mutation removing the last 10 amino acids of the GDS(L) [ Gly, Asp, Ser, (Leu)]-motif esterase/lipase HvGDSL1. We used severe and moderate HvGDSL1 alleles to show that complete HvGDSL1 function is essential for leaf blade cuticular integrity, wax bloom deposition over inflorescences and leaf sheaths and pericarp cuticular ridge formation. Expression data suggest that HvGDSL1 may regulate hull adhesion independently of HvNUD. We found high conservation of HvGDSL1 among barley germplasm, so variation in HvGDSL1 unlikely leads to grain skinning in cultivated barley. Taken together, we reveal a single locus which controls adaptive cuticular properties across different organs in barley. [ABSTRACT FROM AUTHOR]

We studied the effect of the fractured starch granule (fra) mutation on the grain quality of both waxy and non-waxy barley using 185 cross-progeny F6–F7 lines derived from a cross of the non-waxy/fra line Shinkei G073F × the waxy/wild-type cultivar Haneumamochi. In lines containing the fra mutation, the steely grain rate and grain hardness decreased in non-waxy barley but increased in waxy barley, and β-glucan content, protein content, and hollow grain rate increased in both waxy and non-waxy lines. Improved growing conditions may be needed to mitigate the hollow grain rate, which increases the grain crushing rate. In both waxy/fra and non-waxy/fra mutant lines, grain hardness was correlated positively with β-glucan content and steely grain rate and negatively with hollow grain rate. These findings suggest that fra mutant lines with a high grain hardness could be selected to obtain fra lines with low hollow grain rates in both waxy and non-waxy barley. [ABSTRACT FROM AUTHOR]

In the current conditions of intensification of grain production, it is important to develop resource-saving cultivation technologies that ensure the maximum realisation of the genetic potential of crop varieties through the use of foliar fertilisation and mineral fertilisers. The article presents the results of research on the optimisation of fertilisation of spring barley plants. During the period of research, the influence of fertilisation and foliar feeding on the germination and preservation of spring barley plants, net photosynthetic productivity, elements of the yield structure, yield and grain quality was assessed. The research was conducted in the experimental field of Vinnytsia National Agrarian University during 2018--2020 on grey forest medium loamy soils. The hydrothermal conditions were quite contrasting and differed from the average perennial conditions both in terms of heat intensity and moisture level, which allowed to study the influence of the studied factors and their interaction on the formation of spring barley yield and grain quality. The maximum indicators of plant preservation, net productivity of photosynthesis, elements of crop structure, yield and grain quality were obtained in the experiment variant with the combined application of mineral fertiliser at a dose of N30P30K30 and foliar feeding of plants with Vuksal microfertiliser (BBCH 33-51) and (BBCH 51-54) in Aizhan and Aristey varieties. With the application of mineral fertiliser at a dose of N30P30K30, the grain yield of spring barley varieties Aizhan and Aristey increased by 0.99; 0.85 Mg·ha-1, and the protein content by 1.9; 2.0% compared to the control variant. With the combined application of mineral fertiliser at a dose of N30P30K30 and foliar feeding of plants with Vuksal microfertiliser, grain yield increased by 1.54; 1.23 Mg·ha-1 for one-time foliar feeding; by 1.77; 1.42 Mg·ha-1 for two-time foliar feeding, and protein content by 2.8; 2.7 and 3.4; 3.3% in Aizhan and Aristey varieties compared to the control variant, respectively. [ABSTRACT FROM AUTHOR]

The germination process of cereal and legumes has been used to improve their nutritional value. In this study, sorghum, oat, mung bean and barley were germinated for 96 hours. Dry matter, total ash content and phenolic content analyses of germinated samples were performed. Sprouts of germinated grains and legumes were dried and ground into flour. The obtained flour was used in the production of bread (20-40%), cake (5-15%) and rice pudding (10%). In the prepared bread, % baking loss and specific volume were calculated, and sensory analyses of rice pudding, glutenfree cake and bread samples were carried out. All samples' dry matter contents except sorghum decreased by the germination process. Germination caused a slight increase in ash contents of all samples except for barley. The total phenolic contents of mung bean and barley significantly increased during germination. All sprout flours added at 40% reduced the specific volume of the bread. Based on the results, it can be concluded that products with a substitution of sorghum sprout flour were more acceptable in sensorial properties compared to other samples. It can be concluded that it is feasible to produce high nutritional value tasty functional foods with germinated sorghum flour supplementation. [ABSTRACT FROM AUTHOR]

Introduction: Induced modification of plant gene expression is of both fundamental and applied importance. Cis-acting regulatory elements (CREs) are major determinants of the spatiotemporal strength of gene expression. Yet, there are few examples where induced genetic variation in predetermined CREs has been exploited to improve or investigate crop plants. Methods: The digital PCR based FIND-IT technology was applied to discover barley mutants with CRE variants in the promoter of the nutritional important barley grain phytase (PAPhy_a) gene. Results and discussion: Mutants with higher or lower gene expression and ultimately higher or lower mature grain phytase activity (MGPA), respectively, were discovered. Field trials and inositol phosphate profiling during germination showed that PAPhy_a does not influence agronomic performance under the trial conditions but it does shorten the lag time of phosphate mobilization during germination. Higher endogenous MGPA is an improvement of grain quality for feed use as it improves the phosphate bioavailability for monogastric animals. Moreover, as the targeted CRE motifs of the PAPhy_a promoter are shared with a range of seed expressed genes like key cereal and legume storage genes, the current results demonstrates a concept for modulating individual gene expression levels of a range of seed genes. [ABSTRACT FROM AUTHOR]

Barley grains are highly susceptible to fungal contamination. Fungal infection of its kernels occurs in the field and is often associated with Fusarium species, which are phytopathogenic fungi. These fungi cause variety of diseases such as Fusarium head blight (FHB). FHB is one of the most studied diseases, which is responsible for worldwide losses in small-grain cereals. Losses are manifested as reduction in yield and quality of the grain, and the presence of mycotoxins in grain. Moreover, mycotoxins produced by the FHB pathogens are one of the most important groups of anti-nutritional substances found in feed. They are hazardous to animal and human health. Cases of mycotoxicosis caused by ingestion of barley contaminated with FHB mycotoxins have necessitated the need for resistant barley cultivars. That can limit mycotoxin production by the dominant causal pathogen, Fusarium graminearum. Various inoculation methods have been used to decrease the severity of the disease. Resistant cultivars and proper management of prevention methods could reduce damage from FHB. This review examines the progress of FHB resistance in barley, as well as, prevention methods. A combination of cultural practices, planting resistant varieties, chemical control, biological control and harvesting strategies is required. Combining these aspects leads to quality grain production and protects human and animal health. [ABSTRACT FROM AUTHOR]

The experiment studied the effectiveness of fertilizers in the foliar application of Rhizum, Leanum, and Gumisil-A in the field of barley which contain organic substances, macro- and micronutrients, amino acids, phytohormones. Of the three fertilizers, only Leanum contained nitrogen-fixing, phosphorus-forming, and potassium-mobilizing, lactic acid bacteria, decomposers, beneficial fungi. Soil of the experimental plot has low content of humus and nitrogen, medium content of labile phosphorus and exchangeable potassium with pH H2O 6.7. The highest yield was obtained with Leanum, exceeding the control without foliar application by 10.2%. As a result, using Leanum, the level of profitability is 6.44%. The application of Rhizum and Gumisil-A was not profitable due to the insignificant increase in yield and the cost of preparation, transportation and applying. Expensive fuel lubricating materials currently offset farmers' profits. [ABSTRACT FROM AUTHOR]

The Upper Cheliff plain (North of Algeria) experienced substantial impacts from climate variability, particularly notable as the majority of cereal cultivation areas rely on rainfed practices, devoid of irrigation. Certainly, the insufficient rainfall during the critical phases of the crop's life cycle in 2020-2021 resulted in cereal production in this region dropping to 51500 kg, which is less than half of the yield from the 2019-2020 agricultural season (124300 kg). The study aimed to assess the influence of climate change on cereal crop yields in the Upper Cheliff Plain by analyzing trends in rainfall, temperature, and humidity time series over 30 years, from 1981 to 2020. This was established with mathematical formulas and statistical assessments of cereal production. The study revealed three distinct periods: 1981-1987, 1988-2004, and 2005-2020. Annual rainfall decreased by 11% between 1988-2004, while temperatures increased by 0.1°C in recent decades, leading in lower humidity. In 2017/2018, with a rainfall of 634 mm, the average yields of bread wheat (Triticum aestivum L), durum wheat (Triticum durum L) and barley (Hordeum vulgare L) were respectively 2800, 3100 and 2100 kg ha-1. In 1996/1997, when rainfall was less than 246 mm, yields of the same species did not exceed 600, 700, and 700 kg ha-1. It can be explained by the vulnerability of cereals to water stress. The results illustrate the strong link between selected climatic parameters and agricultural production in the semi-arid climate such as the Upper Cheliff Plain. [ABSTRACT FROM AUTHOR]

Copyright of Fermentation Industry / Kvasný Průmysl is the property of Vyzkumny Ustav Pivovarsky a Sladarsky and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Grain size is a key component of grain yield in cereals. It is a complex quantitative trait controlled by multiple genes. Grain size is determined via several factors in different plant development stages, beginning with early tillering, spikelet formation, and assimilates accumulation during the pre-anthesis phase, up to grain filling and maturation. Understanding the genetic and molecular mechanisms that control grain size is a prerequisite for improving grain yield potential. The last decade has brought significant progress in genomic studies of grain size control. Several genes underlying grain size and weight were identified and characterized in rice, which is a model plant for cereal crops. A molecular function analysis revealed most genes are involved in different cell signaling pathways, including phytohormone signaling, transcriptional regulation, ubiquitin–proteasome pathway, and other physiological processes. Compared to rice, the genetic background of grain size in other important cereal crops, such as wheat and barley, remains largely unexplored. However, the high level of conservation of genomic structure and sequences between closely related cereal crops should facilitate the identification of functional orthologs in other species. This review provides a comprehensive overview of the genetic and molecular bases of grain size and weight in wheat, barley, and rice, focusing on the latest discoveries in the field. We also present possibly the most updated list of experimentally validated genes that have a strong effect on grain size and discuss their molecular function. [ABSTRACT FROM AUTHOR]

Drying conditions greatly affect the quality of grains, and efforts should be made to minimize the loss of grains during the drying process. In this study, numerical simulations were conducted to investigate the drying behavior of barley at both the particle and grain pile scales by solving the coupled heat and moisture equation. The model was validated using thin‐layer drying experimental results from the literature, and it was found that the simulated values of average moisture content of the grain had a maximum relative error of 7.73% compared to the experimental values. Furthermore, the impact of air temperature and relative humidity on drying was analyzed. The results showed that the heat and mass transfer coefficients of barley grains varied along the surface, the irregularity of the barley grains resulted in an asymmetric distribution of the surface heat transfer coefficient. Regions with lower porosity in the grain pile exhibited lower rates of heat and moisture transfer, particularly in the central area. Moisture transfer within the grain pile dominated the drying time compared to heat transfer. It was also observed that air temperature and relative humidity were the main variables influencing the drying rate of the grains. As the inlet air temperature increased from 45 to 70°C, the drying rate of the grain pile increased from 0.068% to 0.085%(d.b.)/min. Similarly, reducing the relative humidity from 40% to 18% resulted in an increase in the drying rate from 0.062% to 0.075%(d.b.)/min (within the first 10 min). Practical Applications: This study employed numerical simulations to investigate the drying behavior of barley at both the particle scale and grain pile scale. The developed model was validated using experimental data from thin‐layer drying studies, confirming a significant agreement between the simulated and experimental values. Moreover, the study unveiled the impact of air temperature and relative humidity on the drying process. It was observed that regions with lower porosity within the grain pile experienced reduced rates of heat and moisture transfer. Notably, moisture transfer dominates the drying time. Additionally, the study emphasized the substantial influence of air temperature and relative humidity on the drying rates. These findings offer valuable insights for optimizing drying parameters, including air temperature and relative humidity, in order to enhance the efficiency and quality of the barley grain drying process. [ABSTRACT FROM AUTHOR]

Celiac disease is a global disease requiring genetic susceptibility and gluten exposure to trigger immune‐mediated enteropathy. The effect of the degree of gluten‐containing grain availability on celiac disease prevalence is unknown. Our objective was to compare country‐based gluten availability to celiac prevalence using a systematic literature review. We searched MEDLINE, Embase, Cochrane, and Scopus until May 2021. We included population‐based serum screening with confirmatory testing (second serological study or small intestine biopsy) and excluded specific, high‐risk, or referral populations. We determined country‐specific gluten availability using the United Nations food balance for wheat, barley, and rye. Human leukocyte antigen (HLA) frequencies were obtained from allelefrequencies.net. The primary outcome was association between gluten‐containing grain availability and celiac disease prevalence. Generalized linear mixed models method with Poisson's link was used for analysis. We identified 5641 articles and included 120 studies on 427 146 subjects from 41 countries. Celiac disease prevalence was 0–3.1%, median 0.75% (interquartile range 0.35, 1.22). Median wheat supply was 246 g/capita/day (interquartile range 214.8, 360.7). The risk ratio (RR) for wheat availability on celiac disease was 1.002 (95% confidence interval [CI]: 1.0001, 1.004, P = 0.036). A protective association was seen with barley, RR 0.973 (95% CI: 0.956, 0.99, P = 0.003), and rye, RR 0.989 (95% CI: 0.982, 0.997, P = 0.006). The RR for gross domestic product on celiac disease prevalence was 1.009 (95% CI: 1.005, 1.014, P < 0.001). The RR for HLA‐DQ2 was 0.982 (95% CI: 0.979, 0.986, P < 0.001), and that for HLA‐DQ8 was 0.957 (95% CI: 0.950, 0.964, P < 0.001). In this geo‐epidemiologic study, gluten‐containing grain availability showed mixed associations with celiac disease prevalence. [ABSTRACT FROM AUTHOR]

The objective of this study was to determine the effects of substituting barley grain with short-season, high-moisture shelled corn, and barley grain and barley silage with snaplage on ruminal fermentation and the site and extent of digestion in beef cattle fed finishing diets. Six ruminally and duodenally cannulated heifers (420 ± 16.4 kg body weight) were fed a barley grain and silage finishing diet (BG), a diet where half of the barley grain was replaced with high-moisture shelled corn (HC), or a diet where the barley silage and a portion of grain were replaced with snaplage (SN) in a replicated 3 × 3 Latin square design. While dry matter and starch intake were unaffected, feeding SN resulted in greater (P = 0.02) ruminal but not total tract starch digestibility than BG. Ruminal pH did not differ between HC and BG, but SN reduced (P = 0.02) minimum ruminal pH relative to BG. Feeding BG increased (P = 0.04) ruminal ammonia concentrations over HC. In conclusion, high-moisture shelled corn can partially replace barley grain with minimal impact on nutrient digestibility or ruminal fermentation, but replacement of barley silage and some barley grain with snaplage may increase the risk of ruminal acidosis. [ABSTRACT FROM AUTHOR]

Malting barley (Hordeum vulgare L.) productivity and grain quality are of critical importance to the malting and brewing industry. In this study, we analyzed 12 malting barley genotypes across 8 locations in California and 3 years (2017–2018, 2018–2019, and 2020–2021). The effects of genotype (G), location (L), year (Y), and their interactions were assessed on grain yield (kg ha−1), grain protein content (%), individual‐grain weight (mg), thousand kernel weight (TKW; g), grain size (plump and thin; %), onset gelatinization temperature (GT; temperature at which starch starts to gelatinize), peak GT, offset GT, difference between onset and peak GT, and difference between peak and offset GT. L, Y, and their interaction explained the largest variance for all traits except TKW, peak GT, and difference between onset and peak GT, for which G explained the largest variance. Yield and plump (%) were weakly negatively correlated with onset and peak GT (Pearson's r of −0.15 to −0.21) but showed a positive correlation with the difference between peak and offset GT (Pearson's r of 0.37 and 0.36). The 2020–2021 samples formed partially distinct clusters in principal component analysis, mainly discriminated by high percentage of thins and high onset GT. These findings illustrate the key roles of G, L, and Y in determining malting barley productivity and quality. Core Ideas: Genotype, location, and year variably explain quality traits—for example, grain protein content, gelatinization temperature.The L × Y interaction explained the largest variance for GPC and yield.G and L × Y (and Y for onset) explained the largest variance in onset, peak, and offset GT.The 2020–2021 samples formed partially distinct clusters, segregated by high thins and onset GT.Plumper grains had a lower onset GT but a larger difference between peak and offset GT. [ABSTRACT FROM AUTHOR]

Significant innovations have occurred over the past 50 years in the malting and brewing industries, focused on optimization of the beer mashing, boiling and fermentation processes. One of the challenges faced in beer brewing has been in the malting process to obtain the desired malt and wort quality to produce high-quality beer products. The hydrolytic enzymes produced during grain germination are mostly entrapped inside the cellular matrices of the grain. The intra-grain diffusion of enzymes for in-situ hydrolysis, as well as diffusion of enzymes to wort, depends upon the malt size and malt size fractions obtained after milling. This review investigates the relationship between varying barley grain particle size distribution and the efficiency of the malting and mashing processes. Recommended ideal particle size of barley grain before and after milling are proposed based on the review of existing literature. Each brewing batch of grains with a proportion of >80% plump grains (>2.5 mm in size) is suggested to be the optimal size before milling, whereas the optimum grain particle size after milling ranged between 0.25 and 0.5 mm. The current review will summarize the theoretical aspects for malt milling and the particle size characteristics for optimizing the brewing process. [ABSTRACT FROM AUTHOR]

The grains of three barley varieties were milled and sieved to obtain respective milling fractions with a content of beta-glucans (b-G) from 1.4 to 10.7%. The enriched fraction obtained by the extraction and precipitation contained 24.7% of b-G. The differences between the ratio of stable C carbon isotopes were established. Milling fractions with coarse particles had more beta-glucans and a more negative ratio of δ13C isotope in comparison to the respective intact barley grain. However, the enriched fraction had a less negative isotope ratio. So, it is not expected that the deviation from the stable isotope ratio of grain in milling fractions is the result of the content of b-G, but it depends on other barley grain constituents. In different parts of barley grain, there are substances with different stable isotope ratios, and by milling and sieving, they are assorted to the same milling fraction with most of the b-G. The method for determining the ratio of a stable carbon isotope in diverse barley grain fractions, applied in this investigation, is potentially opening the possibility for an additional method of screening the concentration of bioactive constituents in barley grain. [ABSTRACT FROM AUTHOR]

Beneficial dietary and pro-health values, have resulted in the increasing consumption importance of barley. Therefore, genotypes and cultivation methods are sought that guarantee high functional value of grain. The aim of the study was to assess the content of phenolic acids, flavonoids, chlorophylls, anthocyanidins, phytomelanin and antioxidant activity of grain of three barley genotypes depending on agricultural technology. Two of them are primary genotypes with dark grain pigmentation Hordeum vulgare L. var nigricans and H. vulgare L. var. rimpaui, the third is a modern cultivar 'Soldo' H. vulgare with yellow grain, which is the control sample. Evaluated the effect of foliar application of a amino-acids biostimulant on the functional properties of grain under the conditions of organically and conventionally cultivations. The results indicated a higher antioxidant activity and the concentration of phenolic acids, flavonoids and phytomelanin in the black-grain genotypes. Organic cultivation and application of amino acids had increased the content of phenolic compounds in grain. The antioxidant activity was correlated with the content of syringic acid, naringenin, quercetin, luteolin and phytomelanin. Organically cultivation and the foliar application of an amino acid biostimulant improved the functional properties of barley grain, in particular the original, black-grained genotypes. [ABSTRACT FROM AUTHOR]

Field research was conducted in Poland in 2019–2021 to determine the effect of microbial products and living mulches on grain yield and grain yield structure elements as well as the biological index of soil fertility (BIF) in spring barley grown in organic agriculture. Two factors were examined: I. microbial products: control (no treatment with microbial products), inoculation with phosphorus-releasing bacteria (Bacillus megaterium var. phosphaticum, Arthrobacter agilis), and co-inoculation (simultaneous inoculation) with phosphorus-releasing bacteria (Bacillus megaterium var. phosphaticum, Arthrobacter agilis) and nitrogen-fixing bacteria (Azospirillum lipoferum Br17, Azotobacter chroococcum); II. living mulch: control (no living mulch—spring barley grown in a pure stand), red clover, red clover and Italian ryegrass, and Italian ryegrass. The study results demonstrated that the highest grain yield at 4.5 t ha−1 with superior structure was produced by spring barley following co-inoculation with phosphorus-releasing bacteria (Bacillus megaterium var. phosphaticum, Arthrobacter agilis) and nitrogen-fixing bacteria (Azospirillum lipoferum Br17, Azotobacter chroococcum). The highest value of the biological index of soil fertility (BIF II) determined at the flowering stage was obtained in plots with spring barley cultivated with the living mulch of red clover mixed with Italian ryegrass or red clover following inoculation with phosphorus-releasing bacteria and nitrogen-fixing bacteria 6.9 and 5.7, respectively. [ABSTRACT FROM AUTHOR]

Barley (Hordeum vulgare) grain stands out among other cereals due to its high nutritional value. It results mainly from the high content of fiber and antioxidants, such as phenolic compounds. Barley grains can also be an important source of unsaturated fatty acids and carotenoids that are beneficial to health. This study assessed the effect of the foliar application of an amino-acid biostimulant on the content of phenolic compounds, carotenoids, and the composition of fatty acids in the grain of alternative, black-grain barley genotypes, and the commonly used 'Soldo' cultivar, grown in conventional and organic farming systems. The dark-pigmented grains contained significantly more phenolic acids and flavonoids than the yellow seed of the traditional cultivar and were characterized by a significantly higher proportion of unsaturated fatty acids. The application of the biostimulant significantly increased the concentration of phenolic compounds in grains, especially of alternative genotypes. [ABSTRACT FROM AUTHOR]

Copyright of Harran Journal of Agricultural & Food Science is the property of Harran University, Faculty of Agriculture and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Among a number of climate-related factors, moisture has the greatest impact on crop productivity. In recent years, certain changes have been observed under conditions of the Forest-Steppe of Ukraine with regard to precipitation - from low to in some cases - abnormally high, which requires the study of their impact on the yield and safety of cereal grain for its forecasted production. The article examined the effect of a high level of soil moisture (256.2-272.5 mm) and a low level (47.4-52.3 mm) during the growing season (germination-earing) of spring barley grain on the accumulation of heavy metals in it and its productivity under the conditions of gray forest soils of the Right Bank Forest Steppe of Ukraine. Spring barley varieties Helios and Caesar were selected for the research. A decrease in the accumulation coefficient at a high level of soil moisture (256.6-272.5 mm) in spring barley grain Pb from 8.3% to 11.3%, Cd - from 35.0% to 35.5%, Zn was established - by 15% and Cu - from 11.2% to 16.6% compared to the low level of soil moisture (47.1 mm - 53.3 mm). At the same time, it was found that with a high level of soil moisture, there is a decrease in the yield of Helios and Caesar spring barley by 18.0% and 14.1%, respectively. [ABSTRACT FROM AUTHOR]

Cereal grains have been domesticated largely from food grains to feed and malting grains. Barley (Hordeum vulgare L.) remains unparalleled in its success as a primary brewing grain. However, there is renewed interest in "alternative" grains for brewing (and distilling) due to attention being placed on flavor, quality, and health (i.e., gluten issues) aspects that they may offer. This review covers basic and general information on "alternative grains" for malting and brewing, as well as an in-depth look at several major biochemical aspects of these grains including starch, protein, polyphenols, and lipids. These traits are described in terms of their effects on processing and flavor, as well as the prospects for improvement through breeding. These aspects have been studied extensively in barley, but little is known about the functional properties in other crops for malting and brewing. In addition, the complex nature of malting and brewing produces a large number of brewing targets but requires extensive processing, laboratory analysis, and accompanying sensory analysis. However, if a better understanding of the potential of alternative crops that can be used in malting and brewing is needed, then significantly more research is required. [ABSTRACT FROM AUTHOR]

Simple Summary: The price of feed ingredients has been drastically increasing over the last decade, and thus the methods for improving nutrient digestibility and utilization with various feed ingredients have been widely studied in the field of the swine industry. One of the most efficacious approaches to enhancing nutrient digestibility and utilization is the manipulation of feed particle size. Nonetheless, limited research has been conducted on the impact of varying particle sizes of feeds with high fiber content. Dietary fiber is composed of several components, including β-glucan, arabinoxylan, and cellulose, which exhibit distinct physiological effects on the small and large intestines. The compositions and proportions of these components can influence and modulate nutrient digestibility in the small and large intestines of pigs. The objective of the present investigation was to examine the potential for enhanced nutrient and fiber digestibility in growing pigs through the reduction of particle size in barley and wheat diets. Similar ileal and total nutrient digestibility were observed in pigs fed diets containing fine and coarse wheat; however, pigs consuming coarse barley exhibited reduced nutrient digestibility compared to the other diets. Consequently, the present study shows that nutrient digestibility was more influenced by reduced particle size in barley than wheat, most likely because of the rigid fiber structure of barley and barley hull. The objective of this investigation was to study the effects of different cereal types, barley and wheat, with different particle sizes (PS) on the recovery of ileal digesta and fecal excretion, digestion of nutrients and fiber components, mean transit time (MTT), and short-chain fatty acid content and composition in growing pigs studied in two experiments. Five barrows with ileal cannulas (initial BW 35.9 ± 1.5 kg) in Experiment 1 and thirty-two castrated pigs (30.8 ± 1.3 kg) in Experiment 2 were fed four different diets: barley fine, barley coarse, wheat fine and wheat coarse diets. The cereal type and PS did not influence the relative weight of the small and large intestines and pH of digesta, whereas MTT in the large intestine of pigs fed the coarse barley diet was lower compared to pigs fed other diets (p < 0.05). Pigs fed the coarse barley diet had lower apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of nutrients and fiber (p < 0.05), whereas pigs fed the fine barley diet had similar AID and ATTD to pigs fed wheat fine and coarse diets (p < 0.05). In conclusion, the barley diet was more influenced by PS in comparison to wheat, thereby inducing lower AID and ATTD of nutrient. [ABSTRACT FROM AUTHOR]

The safety and quality of post-production residues is essential before they can be reused. Both to explore the possibility of reuse as a fermentation medium and the context of pathogens' inactivation, the research aimed to characterize the fermentation system of L. lactis ATCC 11454 and brewer's spent grain, malt and barley, especially to in situ inactivation of selected Bacillus strains during the fermentation and storage. Barley products were milled, autoclaved, hydrated and fermented with L. lactis ATCC 11454. Then, the co-fermentation with Bacillus strains was carried out. The amount of polyphenols in the samples ranged from 483.5 to 718.4 ug GAE g−1 and increased after 24 h fermentation with L. lactis ATCC 11454. The high viability of LAB in the fermented samples and after 7 days of storage at 4 °C (8 log CFU g−1) indicates the high nutrients bioavailability during the storage. Also, this co-fermentation on different barley products indicated a high reduction level (2 to 4 logs) of Bacillus due to the biosuppression effect of the LAB strain in this fermentation system. Brewer's spent grain (BSG) fermented with L. lactis ATCC 25 11454 produces a highly effective cell-free supernatant (CFS) for suppressing Bacillus strains. This was evident in both the inhibition zone and fluorescence analysis of bacteria viability. In conclusion, the obtained results justify the use of brewer's spent grain in selected food products, increasing their safety and nutritional value. This finding is highly beneficial in the sustainable management of post-production residues when current waste material can still serve as a source of food. [ABSTRACT FROM AUTHOR]

Even though Sugars Will Eventually be Exported Transporters (SWEETs) have been found in every sequenced plant genome, a comprehensive understanding of their functionality is lacking. In this study, we focused on the SWEET family of barley (Hordeum vulgare). A radiotracer assay revealed that expressing HvSWEET11b in African clawed frog (Xenopus laevis) oocytes facilitated the bidirectional transfer of not only just sucrose and glucose, but also cytokinin. Barley plants harboring a loss-of-function mutation of HvSWEET11b could not set viable grains, while the distribution of sucrose and cytokinin was altered in developing grains of plants in which the gene was knocked down. Sucrose allocation within transgenic grains was disrupted, which is consistent with the changes to the cytokinin gradient across grains, as visualized by magnetic resonance imaging and Fourier transform infrared spectroscopy microimaging. Decreasing HvSWEET11b expression in developing grains reduced overall grain size, sink strength, the number of endopolyploid endosperm cells, and the contents of starch and protein. The control exerted by HvSWEET11b over sugars and cytokinins likely predetermines their synergy, resulting in adjustments to the grain's biochemistry and transcriptome. [ABSTRACT FROM AUTHOR]

While chicken eggs contain many nutrients necessary for humans and there are various cooking methods, the nutritional components are used as they are, and there are no traditional foods that utilize microorganisms. Koji-mold, containing Aspergillus oryzae , A. sojae , and A. luchuensis , which has been used in various fermented foods since ancient times, grows on raw grain materials such as rice and barley to become koji. This can give flavors not found in the raw materials that can decompose and convert the nutritional components of the raw materials. Here, we succeeded for the first time in developing egg-koji that uses only eggs and koji-mold by selecting and combining cooked egg powder (CEP) and A. oryzae AO101 as the most suitable combination. To suppress the explosive growth of harmful bacteria, we improved the sterilization method, watering method, and amount of water. In addition, it was found that egg-koji has a characteristic enzyme activity balance, in which amylase is extremely low and protease at pH 6 was high compared to grain koji, such as rice and barley. Egg-koji might produce enzymes suitable for taking in nutrients when growing into CEP and would be expected to give a flavor that could not be achieved by cooking or additives. [ABSTRACT FROM AUTHOR]

The phenolic acids, ferulic acid and p-coumaric acid, are components of plant cell walls in grasses, including many of our major food crops. They have important health-promoting properties in grain, and influence the digestibility of biomass for industrial processing and livestock feed. Both phenolic acids are assumed to be critical to cell wall integrity and ferulic acid, at least, is important for cross-linking cell wall components, but the role of p-coumaric acid is unclear. Here we identify alleles of a BAHD p-coumaroyl arabinoxylan transferase, HvAT10, as responsible for the natural variation in cell wallesterified phenolic acids in whole grain within a cultivated two-row spring barley panel. We show that HvAT10 is rendered non-functional by a premature stop codon mutation in half of the genotypes in our mapping panel. This results in a dramatic reduction in grain cell wall-esterifed p-coumaric acid, a moderate rise in ferulic acid, and a clear increase in the ferulic acid to p-coumaric acid ratio. The mutation is virtually absent in wild and landrace germplasm suggesting an important function for grain arabinoxylan p-coumaroylation pre-domestication that is dispensable in modern agriculture. Intriguingly, we detected detrimental impacts of the mutated locus on grain quality traits where it was associated with smaller grain and poorer malting properties. HvAT10 could be a focus for improving grain quality for malting or phenolic acid content in wholegrain foods. [ABSTRACT FROM AUTHOR]

Since its Neolithic domestication in the Fertile Crescent, barley has spread to all continents and represents a major cereal in many modern agrarian systems. Current barley diversity includes thousands of varieties divided into four main categories corresponding to 2-row and 6-row subspecies and naked and hulled types, each of them with winter and spring varieties. This diversity is associated to different uses and allow cultivation in diverse environments. We used a large dataset of 58 varieties of French origin, (1) to assess the taxonomic signal in barley grain measurements comparing 2-row and 6-row subspecies, and naked and hulled types; (2) to test the impact of the sowing period and interannual variation on the grains size and shape; (3) to investigate the existence of morphological differences between winter and spring types; and finally (4) to contrast the relationship between the morphometric and genetic proximity. Size and shape of 1980 modern barley caryopses were quantified through elliptic Fourier Transforms and traditional size measurements. Our results indicate that barley grains record morphological diversity of the ear (89.3% classification accuracy between 2-row/6-row subspecies; 85.2% between hulled and naked type), sowing time of the grains (from 65.6% to 73.3% within barley groups), and environmental conditions during its cultivation and varietal diversity. This study opens perspectives for studying archaeological barley seeds and tracing the barley diversity and evolution since the Neolithic. [ABSTRACT FROM AUTHOR]

Featured Application: A predictive model combining a genetic algorithm and a B-spline curve designed to assess the mycological state of malting barley grain, which could be used as an effective tool supporting modern systems of postharvest grain preservation and storage. Postharvest grain preservation and storage can significantly affect the safety and nutritional value of cereal-based products. Negligence at this stage of the food processing chain can lead to mold development and mycotoxin accumulation, which pose considerable threats to the quality of harvested grain and, thus, to consumer health. Predictive models evaluating the risk associated with fungal activity constitute a promising solution for decision-making modules in advanced preservation management systems. In this study, an attempt was made to combine genetic algorithms and B-spline curves in order to develop a predictive model to assess the mycological state of malting barley grain stored at various temperatures (T = 12–30 °C) and water activity in grain (aw = 0.78–0.96). It was found that the B-spline curves consisting of four second-order polynomials were sufficient to approximate the datasets describing fungal growth in barley ecosystems stored under steady temperature and humidity conditions. Based on the designated structures of B-spline curves, a universal parameterized model covering the entire range of tested conditions was developed. In the model, the coordinates of the control points of B-spline curves were modulated by genetic algorithms using values of storage parameters (aw and T). A statistical assessment of model performance showed its high efficiency (R2 = 0.94, MAE = 0.21, RMSE = 0.28). As the proposed model is based on easily measurable on-line storage parameters, it could be used as an effective tool supporting modern systems of postharvest grain treatment. [ABSTRACT FROM AUTHOR]

The crop seeds have been a staple food for humans, and seed yield is important for sustaining agriculture development and enhancing human adaptability to food risks. The phenomenon of pre-harvest sprouting (PHS), caused by seed dormancy deficiency, and the phenomenon of low seedling emergence caused by seed deep dormancy, will lead to a reduction in agricultural production. Therefore, it is particularly important to understand the regulation mechanisms of seed dormancy. There are many studies on the regulation of seed dormancy in rice, but there are few studies on the regulation of seed dormancy in other crops, and the research on its mechanism is not thorough enough. In this paper, we comprehensively summarize the regulation mechanisms of cereal seed dormancy, including rice, barley and wheat, discussing the integral mechanism of seed dormancy. This information should provide new insights for developing versatile cultivated lines to improve crop yield and economic benefits. [ABSTRACT FROM AUTHOR]

The plant microbiota fulfils various crucial functions related to host health, fitness, and productivity. Over the past years, the number of plant microbiome studies continued to steadily increase. Technological advancements not only allow us to produce constantly increasing datasets, but also to extract more information from them in order to advance our understanding of plant-microbe interactions. The growing knowledge base has an enormous potential to improve microbiome-based, sustainable agricultural practices, which are currently poorly understood and have yet to be further developed. Cereal plants are staple foods for a large proportion of the world's population and are therefore often implemented in microbiome studies. In the present review, we conducted extensive literature research to reflect the current state of knowledge in terms of the microbiome of the four most commonly cultivated cereal plants. We found that currently the majority of available studies are targeting the wheat microbiome, which is closely followed by studies on maize and rice. There is a substantial gap, in terms of published studies, addressing the barley microbiome. Overall, the focus of most microbiome studies on cereal plants is on the below-ground microbial communities, and there is more research on bacteria than on fungi and archaea. A meta-analysis conducted in the frame of this review highlights microbiome similarities across different cereal plants. Our review also provides an outlook on how the plant microbiota could be harnessed to improve sustainability of cereal crop production. [ABSTRACT FROM AUTHOR]

Summary: In cereal species, grain size is influenced by growth of the ovule integuments (seed coat), the spikelet hull (lemma and palea) and the filial endosperm. Whether a highly conserved ovule tissue, the nucellus, has any impact on grain size has remained unclear.Immunolabelling revealed that the barley nucellus comprises two distinct cell types that differ in terms of cell wall homogalacturonan (HG) accumulation. Transcriptional profiling of the nucellus identified two pectin methylesterase (PME) genes, OVULE PECTIN MODIFIER 1 (OPM1) and OPM2, which are expressed in the unfertilized ovule but absent from the seed.Ovules from an opm1 opm2 mutant and plants expressing an ovule‐specific pectin methylesterase inhibitor (PMEI), exhibit reduced HG accumulation. This results in changes to ovule cell size and shape and ovules that are longer than wild‐type (WT) controls. At grain maturity, this is manifested as significantly longer grain.These findings indicate that cell wall composition during ovule development acts to limit ovule and seed growth. The investigation of ovule PME and PMEI activity reveals an unexpected role of maternal tissues in controlling grain growth before fertilization, one that has been lacking from models exploring improvements in grain size. [ABSTRACT FROM AUTHOR]

To counter projected reductions in yields of the major crop barley, it is essential to elucidate the mechanisms of its resilience. To assist such efforts, we collected grains from plants grown in fields at 12 testing stations, with suitable temperature and precipitation gradients for identifying environmentally induced changes in their protein and metabolite contents. We then subjected the grains to detailed molecular analysis. The results showed that numerous metabolites and at least a quarter of the grain protein content was modulated by the environment, and provided insights into barley seed production under abiotic stress, including alterations in ribosomal proteins, heatshock protein 70 family proteins, inhibitors, storage proteins, and lipid droplet formation. Potential positive and negative markers of yield were also identified, including the phenolic compound catechin and storage protein levels, respectively. Complementary analyses of barley seedlings and Arabidopsis seeds, respectively, confirmed the role of the identified proteins in abiotic stress responses and highlighted evolutionarily conserved mechanisms. In addition, accelerated ageing experiments revealed that variations in the environment had stronger effects on seed longevity than the genotype. Finally, seeds with the highest longevity differed from the others in gibberellin contents, H2O2 metabolism, and levels of >250 proteins, providing novel targets for improving resilience. [ABSTRACT FROM AUTHOR]

This study aimed to investigate the impacts of three organic acid salts and two bacterial additives on the fermentation profile, in vitro digestibility and gas production, and aerobic stability of total mixed ration (TMR) silage prepared with 10% fresh weight (FW) of wet hulless barley distillers' grains (WHDG), 35% FW of common vetch, 15% FW of whole-crop oat, 10% FW of hulless barley straw, and 30% FW of mixed concentrate. The total mixed ration (555 g kg−1 FW) was ensiled with six treatments: (1) no additives (control); (2) calcium propionate (CAP; 0.5% FW); (3) potassium sorbate (POS; 0.1% FW); (4) sodium diacetate (SDA; 0.5% FW); (5) Lactobacillus buchneri (LB; 1 × 106 cfu g−1 FW); and (6) Lactobacillus casei (LAC; 1 × 106 cfu g−1 FW). All silos (20 L) were opened for the fermentation profile and in vitro digestibility analysis after 95 days of fermentation, and then subjected to an aerobic stability experiment for 14 days. All the TMR silage was well preserved with a low pH (4.32~4.51), acceptable levels of butyric acid (1.34~1.56 g kg−1 dry matter), and ammonia nitrogen (69.1~87.1 g kg−1 total nitrogen). All the groups were steady during aerobic exposure, while the SDA treatment was more stable with lower populations of undesirable microorganisms on day 14. The CAP, POS, and SDA treatments evidently (p < 0.05) enhanced the in vitro digestibility of dry matter (54.8~57.5 vs. 48.4%) and neutral detergent fiber (48.4~51.6 vs. 41.1%) compared to the control. By comprehensive consideration, SDA is recommended as additive to enhance fermentation quality, aerobic stability, and in vitro digestibility of TMR silage containing 10% FW of WHDG. [ABSTRACT FROM AUTHOR]

Threshability, defined here as the propensity of grains to lose their hull after harvest, is a key trait in naked barley (Hordeum vulgare L.). While threshability is a defining characteristic of naked grains and has been found to be associated with grain size and shape, its genetic architecture is poorly described. The goals of this study were to identify quantitative trait loci associated with threshability and evaluate their utility as covariates in genomic prediction models. A genome‐wide association study identified two loci on chromosomes 2H and 3H associated with threshability. The locus on chromosome 2H accounted for 9.9% of the phenotypic variance explained (PVE). The locus on chromosomes 3H accounted for 7.8% of the PVE. With effects on threshability of 0.18 and 0.29 for each marker, respectively, these markers could have a limited impact when implemented in marker‐assisted selection. Predictive ability for threshability was 0.842 using a structured genomic best linear unbiased prediction model. Incorporation of the markers with significant associations as covariates in the model did not improve predictive ability. Predictive ability was improved by the use of a multi‐trait model including grain test weight. The high predictive ability for threshability overall indicate that genomic selection would be useful in selection for threshability in naked barley. Core Ideas: Two markers were identified with significant associations to threshability.Predictive ability for threshability was 0.842 using a structured gBLUP model.Predictive ability was not increased by inclusion of identified QTL as covariates.Predictive ability was increased using a multi‐trait prediction model. [ABSTRACT FROM AUTHOR]

Context: Sulfur deficiency is a limiting factor of cereal crops, causing significant crop losses associated with a decrease in the number of grains. In barley (Hordeum vulgare L.) crops, grain number (and grain yield) is associated with spike biomass at anthesis, which is related to crop growth rate during the period preceding anthesis (i.e. the 'critical period') when spike growth occurs. These relationships have been established for various cultivars under varying radiation intensity and in different locations, and have been confirmed in crops grown under nitrogen and phosphorus deficiencies. Aims: The objective of this study was to determine, for malting barley, whether the effects of sulfur, nitrogen and their interaction on the number of grains per unit area could be explained by changes in crop growth rate or photothermal quotient (ratio of intercepted photosynthetically active radiation to temperature) during the critical period. Methods: Three field experiments were conducted in the Pampean region of Argentina to evaluate the effects of sulfur fertilisation under three levels of nitrogen availability on the number of grains per unit area. Key results: Changes in grain number were associated with crop growth rate or photothermal quotient during the critical period. This association was maintained when sulfur fertilisation changed grain number. Crop growth rate during the critical period varied mainly as a result of changes in accumulated intercepted radiation. Conclusions: Sulfur fertilisation increases grain number and grain yield by increasing radiation interception during the critical period, probably as a consequence of increased leaf area. Implications: The determination of grain number under sulfur deficiency is similar to that observed under deficiencies of other nutrients. Deficiencies of nutrients such as sulfur cause significant crop losses associated with a decrease in the number of grains. In this work, we show that reductions in grain number in barley crops caused by sulfur deficiencies are determined by reductions in crop growth in the period prior to heading known as the 'critical period'. This shows that the effects of deficiencies of different nutrients on cereal yields have some common mechanisms. [ABSTRACT FROM AUTHOR]

Different varieties of cereal grains differ in their nutritional composition and product making characteristics. Barley is an underutilized and neglected food crop in Pakistan. Mainly barley is used as source of carbohydrates but is also an imperative source of minerals specially potassium, calcium and phosphorus. Although, the importance of these minor constituents as minerals is of significant importance for human nutrition, the micronutrients of different Pakistani barley varieties have not been studied up till now. The aim of this study was to characterize the mineral profile of Pakistani grown barley varieties from different geographical regions. Ash content in different barley varieties ranged between 1.32-2.00%. Potassium (409-585 mg/100g) and phosphorus (307-446 mg/100g) were found to be highest minerals in all varieties while selenium (0.04-0.30 mg/kg) and cobalt (3.1-24.2 µg/kg) were found in trace amount. Overall, the varieties grown in Khyber Pakhtoonkhwa (KPK) province were found to be potentially good source of minerals especially iron and calcium. However, the varieties grown in Sindh province were found to be poor sources of minerals excluding sodium. Three statistically significant clusters were identified using chemometric tools. Barley variety Rakhshan-10 showed highest ash content and overall presented better mineral profile. Significant differences in mineral content were found in all barley varieties grown in different locations. Overall, findings of this study will help the food industries in selection of barley variety/ies with high mineral profile to be incorporated in barley-based food products. [ABSTRACT FROM AUTHOR]

Barley, oats, or spelt consumed as minimally processed whole grains provide several health benefits, especially when grown under organic field management conditions. Therefore, the effects of organic and conventional farming on the compositional traits (protein, fibre, fat, and ash) of barley, oat, and spelt grains and groats were compared using three winter barley varieties ('Anemone', 'BC Favorit', and 'Sandra'), two spring oat varieties ('Max' and 'Noni'), and three spelt varieties ('Ebners Rotkorn', 'Murska bela', and 'Ostro'). Groats were produced from harvested grains by a combination of threshing, winnowing, and brushing/polishing. Multitrait analysis showed significant differences between species, field management practices, and fractions, with clear compositional differences between organic and conventional spelt. Barley and oat groats had a higher thousand kernel weight (TKW) and β-glucan, but lower crude fibre, fat, and ash contents than the grains. The composition of the grains of the different species differed significantly for more traits (TKW, fibre, fat, ash, and β-glucan) than that of the groats (TKW and fat), while field management only affected the fibre content of the groats and the TKW, ash, and β-glucan contents of the grains. The TKW, protein, and fat contents of the different species differed significantly under both conventional and organic growing conditions, while the TKW and fibre contents of grains and groats differed under both systems. The caloric value of the final products of barley, oats, and spelt groats ranged from 334–358 kcal/100 g. This information will be useful for not only the processing industry, but also for breeders and farmers, and last, but not least, for consumers. [ABSTRACT FROM AUTHOR]

The phenolic acids, ferulic acid and p-coumaric acid, are components of plant cell walls in grasses, including many of our major food crops. They have important health-promoting properties in grain, and influence the digestibility of biomass for industrial processing and livestock feed. Both phenolic acids are assumed to be critical to cell wall integrity and ferulic acid, at least, is important for cross-linking cell wall components, but the role of p-coumaric acid is unclear. Here we identify alleles of a BAHD p-coumaroyl arabinoxylan transferase, HvAT10, as responsible for the natural variation in cell wall-esterified phenolic acids in whole grain within a cultivated two-row spring barley panel. We show that HvAT10 is rendered non-functional by a premature stop codon mutation in half of the genotypes in our mapping panel. This results in a dramatic reduction in grain cell wall-esterifed p-coumaric acid, a moderate rise in ferulic acid, and a clear increase in the ferulic acid to p-coumaric acid ratio. The mutation is virtually absent in wild and landrace germplasm suggesting an important function for grain arabinoxylan p-coumaroylation pre-domestication that is dispensable in modern agriculture. Intriguingly, we detected detrimental impacts of the mutated locus on grain quality traits where it was associated with smaller grain and poorer malting properties. HvAT10 could be a focus for improving grain quality for malting or phenolic acid content in wholegrain foods.

Essential oil (EO)-based nanoemulsions (NEs) are promising grain protectants in the management of stored-product pests. However, the potential impact of the stored-grain species on the green insecticide effectiveness has been poorly studied. In this study, two concentrations of EO-based NEs from Carlina acaulis L., Mentha longifolia (L.) Huds., and Hazomalania voyronii (Jum.) Capuron were evaluated as insecticides against the major stored-product pest Sitophilus oryzae (L.) on barley, oats, and maize kernels. The C. acaulis EO-based NE applied at 1000 ppm on barley achieved the highest mortality, killing 94.4% of S. oryzae adults after a 7-day exposure, followed by 1000 ppm of H. voyronii EO-based NE (83.3%). The lowest mortality (1.1%) was recorded with 500 ppm of M. longifolia EO-based NE on maize after the same interval. All tested NEs exhibited elevated efficacy when applied on barley, while mortalities were lower on oats and maize. Furthermore, C. acaulis EO-based NE was the most effective when applied on all commodities, followed by H. voyronii and M. longifolia EO-based NEs. Overall, our results highlighted the significant impact of the stored cereal on the insecticidal effectiveness of EO-based NE used for stored-product pest control. Sitophilus oryzae adults on barley can be adequately controlled through the application of C. acaulis and H. voyronii EO-based NEs. [ABSTRACT FROM AUTHOR]

This study applied the text frequency method to analyze the crops prevalent during the Chosunwangjoshilrok dynasty, and categorized the results by each king. Contemporary perception of grains was observed by examining the staple crop types. Staple species were examined using the word cloud and semantic network analysis. Totally, 101,842 types of crop consumption were recorded during the Chosunwangjoshilrok period. Of these, 51,337 (50.4%) were grains, 50,407 (49.5%) were beans, and 98 (0.1%) were seeds. Rice was the most frequently consumed grain (37.1%), followed by pii (11.9%), millet (11.3%), barley (4.5%), proso (0.8%), wheat (0.6%), buckwheat (0.1%), and adlay (0.05%). Grain chronological frequency in the Choseon dynasty was determined to be 15,520 cases in the 15th century (30.2%), 11,201 cases in the 18th century (21.8%), 9,421 cases in the 17th century (18.4%), 9,113 cases in the 16th century (17.8%), and 6,082 cases in the 19th century (11.8%). Interest in grain amongst the 27 kings of Choseon was evaluated based on the frequency of records. The 15th century King Sejong recorded the maximum interest with 13,363 cases (13.1%), followed by King Jungjo (8,501 cases in the 18th century; 8.4%), King Sungjong (7,776 cases in the 15th century; 7.6%). [ABSTRACT FROM AUTHOR]

Featured Application: This study shows that the use of the vacuum impregnation process of barley grain for the production of light malt significantly reduces the soaking time of the grain, which speeds up the malt production process without causing significant negative changes in the parameters of wort. Vacuum impregnation is not used in industrial beer production. However, it can be quickly implemented with a small investment, i.e., the purchase of a vacuum pump and vacuum chamber. In this study, the process of the vacuum impregnation of Kangoo barley grain, at the stage of soaking, was used in the production of light malt. The influence of vacuum impregnation on the speed of the water uptake by the barley, at temperatures of 12, 14, 16 and 18 °C, was also analyzed. At this stage of the research, the grain was soaked in water to obtain a moisture content of approximately 42%. The samples for the moisture content tests were taken every 2 h. The grain intended for malt was soaked in an air–water system and was kept submerged in water for 6 h. It was then removed from the water and kept for 18 h. The grain was aerated during the soaking process. The malting and soaking lasted eight days at temperatures of 12, 14, 16 and 18 °C. The samples for further testing were taken daily. Then, each of the samples was dried, following the same procedure. The sprouts were removed immediately after the dried samples contained approximately 4% moisture. Following a 3-month maturation process, the congress wort was produced from the malt. The pH and the extract content in the wort were tested. It was found that the process of vacuum impregnation significantly accelerates the uptake of water by the grain. In almost all cases, the influence of the tested factors on the pH of the wort and the extract content was also observed. [ABSTRACT FROM AUTHOR]

Anaphes (Anaphes) flavipes (Foerster), a fairyfly (Hymenoptera: Mymaridae) native of Europe, is an economically important egg parasitoid for the natural control of Oulema spp. leaf beetle (Coleoptera: Chrysomelidae) pests of cereal crops such as barley, oats, rye, and wheat in Europe, and for the classical biological control of the invasive Oulema melanopus (L.) in North America. A morphologically very similar Anaphes (Anaphes) nipponicus Kuwayama, known from mainland China, Japan, Republic of Korea, Far East of Russia and Taiwan, is an egg parasitoid of Oulema oryzae (Kuwayama), a pest of rice mainly in temperate parts of East Asia. The nuclear 28S-D2 and ITS2 and the mitochondrial COI genes were used as markers to compare specimens of A. (Anaphes) flavipes reared from eggs of an Oulema sp. on barley in Germany with those of A. (Anaphes) nipponicus reared from eggs of O. oryzae on rice in Honshu Island, Japan. Because the resulting sequences are practically identical, within an expected intraspecific genetic variability, conspecificity of these two nominal species has been confirmed, and consequently A. (Anaphes) nipponicus Kuwayama, 1932, syn. n. is synonymized with A. (Anaphes) flavipes (Foerster, 1841). Taxonomic notes and illustrations are provided for the specimens of both sexes of A. (Anaphes) flavipes from Japan to facilitate their recognition. [ABSTRACT FROM AUTHOR]