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Your search keyword '"Huifeng Ke"' showing total 2 results
Start Over Author "Huifeng Ke" Topic gossypium
2 results on '"Huifeng Ke"'

1. SEP-like genes of Gossypium hirsutum promote flowering via targeting different loci in a concentration-dependent manner

Author

Liting Chen, Yuanyuan Yan, Huifeng Ke, Zihao Zhang, Chengsheng Meng, Limei Ma, Zhengwen Sun, Bin Chen, Zhengwen Liu, Guoning Wang, Jun Yang, Jinhua Wu, Zhikun Li, Liqiang Wu, Guiyin Zhang, Yan Zhang, Xingfen Wang, and Zhiying Ma

Subjects
SEP-like gene, Gossypium, flowering time control, cotton, concentration-dependent regulation, Plant culture, SB1-1110
Abstract

SEP genes are famous for their function in the morphological novelty of bisexual flowers. Although the diverse functions of SEP genes were reported, only the regulatory mechanisms underlying floral organ development have been addressed. In this study, we identified SEP-like genes in Gossypium and found that SEP3 genes were duplicated in diploid cotton varieties. GhSEP4.1 and GhSEP4.2 were abundantly transcribed in the shoot apical meristem (SAM), but only GhSEP4.2 was expressed in the leaf vasculature. The expression pattern of GhSEPs in floral organs was conserved with that of homologs in Arabidopsis, except for GhSEP2 that was preponderantly expressed in ovules and fibers. The overexpression and silencing of each single GhSEP gene suggested their distinct role in promoting flowering via direct binding to GhAP1 and GhLFY genomic regions. The curly leaf and floral defects in overexpression lines with a higher expression of GhSEP genes revealed the concentration-dependent target gene regulation of GhSEP proteins. Moreover, GhSEP proteins were able to dimerize and interact with flowering time regulators. Together, our results suggest the dominant role of GhSEP4.2 in leaves to promote flowering via GhAP1-A04, and differently accumulated GhSEP proteins in the SAM alternately participate in forming the dynamic tetramer complexes to target at the different loci of GhAP1 and GhLFY to maintain reproductive growth. The regulatory roles of cotton SEP genes reveal their conserved and diversified functions.

Published
2022
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2. Novel insights into water-deficit-responsive mRNAs and lncRNAs during fiber development in Gossypium hirsutum

Author

Nan Wu, Jun Yang, Guoning Wang, Huifeng Ke, Yan Zhang, Zhengwen Liu, Zhiying Ma, and Xingfen Wang

Subjects
Gossypium, mRNA, Botany, Gossypium hirsutum, Water, Plant Science, Long noncoding RNA (lncRNA), RNA, Plant, QK1-989, RNA, Long Noncoding, Fiber, Cotton Fiber, RNA, Messenger, Water deficit, Research Article
Abstract

Background The fiber yield and quality of cotton are greatly and periodically affected by water deficit. However, the molecular mechanism of the water deficit response in cotton fiber cells has not been fully elucidated. Results In this study, water deficit caused a significant reduction in fiber length, strength, and elongation rate but a dramatic increase in micronaire value. To explore genome-wide transcriptional changes, fibers from cotton plants subjected to water deficit (WD) and normal irrigation (NI) during fiber development were analyzed by transcriptome sequencing. Analysis showed that 3427 mRNAs and 1021 long noncoding RNAs (lncRNAs) from fibers were differentially expressed between WD and NI plants. The maximum number of differentially expressed genes (DEGs) and lncRNAs (DERs) was identified in fibers at the secondary cell wall biosynthesis stage, suggesting that this is a critical period in response to water deficit. Twelve genes in cotton fiber were differentially and persistently expressed at ≥ five time points, suggesting that these genes are involved in both fiber development and the water-deficit response and could potentially be used in breeding to improve cotton resistance to drought stress. A total of 540 DEGs were predicted to be potentially regulated by DERs by analysis of coexpression and genomic colocation, accounting for approximately 15.76% of all DEGs. Four DERs, potentially acting as target mimics for microRNAs (miRNAs), indirectly regulated their corresponding DEGs in response to water deficit. Conclusions This work provides a comprehensive transcriptome analysis of fiber cells and a set of protein-coding genes and lncRNAs implicated in the cotton response to water deficit, significantly affecting fiber quality during the fiber development stage.

Published
2022

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