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Start Over Topic good health and well being
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1. Detection of residual rifampicin in urine via fluorescence quenching of gold nanoclusters on paper

Author

Chatterjee, Krishnendu, Kuo, Chiung Wen, Chen, Ann, and Chen, Peilin

Subjects
Agricultural, Veterinary and Food Sciences, Biological Sciences, Biomedical and Clinical Sciences, Industrial Biotechnology, Medical Biotechnology, Agricultural Biotechnology, Infection, Good Health and Well Being, Animals, Antibiotics, Antitubercular, Biosensing Techniques, Cattle, Gold, Humans, Limit of Detection, Metal Nanoparticles, Paper, Point-of-Care Systems, Rifampin, Serum Albumin, Bovine, Spectrometry, Fluorescence, Fluorescent biosensors, Fluorescence quenching, Gold nanocluster, Rifampicin detection, TB drug monitoring, Wax-printed paper platform, Technology, Nanoscience & Nanotechnology, Agricultural biotechnology, Industrial biotechnology, Medical biotechnology
Abstract

BackgroundRifampicin or rifampin (R) is a common drug used to treat inactive meningitis, cholestatic pruritus and tuberculosis (TB), and it is generally prescribed for long-term administration under regulated dosages. Constant monitoring of rifampicin is important for controlling the side effects and preventing overdose caused by chronic medication. In this study, we present an easy to use, effective and less costly method for detecting residual rifampicin in urine samples using protein (bovine serum albumin, BSA)-stabilized gold nanoclusters (BSA-Au NCs) adsorbed on a paper substrate in which the concentration of rifampicin in urine can be detected via fluorescence quenching. The intensity of the colorimetric assay performed on the paper-based platforms can be easily captured using a digital camera and subsequently analyzed.ResultsThe decreased fluorescence intensity of BSA-Au NCs in the presence of rifampicin allows for the sensitive detection of rifampicin in a range from 0.5 to 823 µg/mL. The detection limit for rifampicin was measured as 70 ng/mL. The BSA-Au NCs were immobilized on a wax-printed paper-based platform and used to conduct real-time monitoring of rifampicin in urine.ConclusionWe have developed a robust, cost-effective, and portable point-of-care medical diagnostic platform for the detection of rifampicin in urine based on the ability of rifampicin to quench the fluorescence of immobilized BSA-Au NCs on wax-printed papers. The paper-based assay can be further used for the detection of other specific analytes via surface modification of the BSA in BSA-Au NCs and offers a useful tool for monitoring other diseases.

Published
2015

2. Small form factor, flexible, dual-modality handheld probe for smartphone-based, point-of-care oral and oropharyngeal cancer screening

Author

Vishal Gupta, Sumsum P. Sunny, Keerthi Gurushanth, Praveen Birur, Rongguang Liang, Kimberly E. Wooten, Sanjana Patrick, Bofan Song, Ross D. Uthoff, Moni A. Kuriakose, Mary E. Platek, Petra Wilder-Smith, Amritha Suresh, Trupti Kolur, and Anurag K. Singh

Subjects
Paper, medicine.medical_specialty, Point-of-Care Systems, Biomedical Engineering, oral cancer screening, Optical Physics, 01 natural sciences, Imaging, autofluorescence imaging, 010309 optics, Biomaterials, Computer-Assisted, Clinical Research, Opthalmology and Optometry, Image Interpretation, Computer-Assisted, 0103 physical sciences, Cancer screening, Medical imaging, medicine, Humans, Medical physics, Dental/Oral and Craniofacial Disease, Image Interpretation, mobile health, Early Detection of Cancer, Point of care, Cancer, business.industry, Prevention, Optical Imaging, Optics, Equipment Design, Health Services, medicine.disease, Atomic and Molecular Physics, and Optics, Telemedicine, 3. Good health, Electronic, Optical and Magnetic Materials, Small form factor, Oropharyngeal Neoplasms, Good Health and Well Being, Dual modality, Mouth Neoplasms, business, biomedical imaging, Mobile device, Camera module
Abstract

Oral cancer is a growing health issue in low- and middle-income countries due to betel quid, tobacco, and alcohol use and in younger populations of middle- and high-income communities due to the prevalence of human papillomavirus. The described point-of-care, smartphone-based intraoral probe enables autofluorescence imaging and polarized white light imaging in a compact geometry through the use of a USB-connected camera module. The small size and flexible imaging head improves on previous intraoral probe designs and allows imaging the cheek pockets, tonsils, and base of tongue, the areas of greatest risk for both causes of oral cancer. Cloud-based remote specialist and convolutional neural network clinical diagnosis allow for both remote community and home use. The device is characterized and preliminary field-testing data are shared.

Published
2019