1. Metal coordination induced SERS nanoprobe for sensitive and selective detection of histamine in serum.
- Author
-
Li P, Zhou B, Ge M, Jing X, and Yang L
- Subjects
- Histamine, Nanotechnology, Reproducibility of Results, Spectrum Analysis, Raman, Gold, Metal Nanoparticles
- Abstract
Sensitivity and credibility detecting histamine (HA) as an important neurotransmitter in biofluids is of importance in analytical science and physiology. Surface-enhanced Raman spectroscopy (SERS) is able to realize the high sensitivity with single molecules level, but providing the high sensitivity for HA with a small cross section remains a challenge. Here we develop the metal complex-based SERS nanoprobe nitrilotriacetic acid-Ni
2+ (NTA-Ni2+ ) combined with self-assemble Au NPs active substrates for sensitive detection of HA. The NTA-Ni2+ can capture the HA molecules close to Au NPs substrates and then amplify the Raman signals of HA owing to the formation of a complex of NTA-Ni2+ -HA. The self-assemble Au film through the evaporation-driven method can provide the high-density hot spots substrate with high stability and reproducibility. The NTA-Ni2+ decorated Au NPs as nanoprobe responds to HA with 1 μM level of sensitivity. More importantly, the developed SERS nanoprobe composing of NTA-Ni2+ and self-assemble Au NPs can be utilized to detect and monitor the HA spiked into serum, indicating the potential prospect in analysis of HA in complex specimen., (Copyright © 2021 Elsevier B.V. All rights reserved.)- Published
- 2022
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