1. A novel nanosensor composed of aptamer bio-dots and gold nanoparticles for determination of thrombin with multiple signals.
- Author
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Kuang L, Cao SP, Zhang L, Li QH, Liu ZC, Liang RP, and Qiu JD
- Subjects
- Humans, Limit of Detection, Metal Nanoparticles ultrastructure, Aptamers, Nucleotide chemistry, Biosensing Techniques methods, Fluorescence Resonance Energy Transfer methods, Gold chemistry, Metal Nanoparticles chemistry, Thrombin analysis
- Abstract
Thrombin is a crucial multifunctional enzyme involved in many physiological and pathological processes. Its detection is of great importance. In this work, a novel bio-dots nanosensor for detection of thrombin with colorimetric, fluorometric and light-scattering signals is developed. This nanosensor is composed of thrombin-binding aptamer bio-dots (TBA-dots) and gold nanoparticles (AuNPs), where TBA-dots serve as fluorometric reporter and AuNPs function as multiple roles as colorimetric reporter, light scattering reporter and fluorescence quencher. TBA-dots retain inherent structures of aptamer to specifically interact with thrombin and simultaneously induce the aggregation of AuNPs. The mechanism of the sensing system is based on distance-dependent aggregation of AuNPs and fluorescence resonance energy transfer (FRET). The nanosensor needs no further surface functionalization required for the as-prepared bio-dots and AuNPs, which provides a sensitive method for the selective detection of thrombin with a detection limit as low as 0.59nM. In addition, it provides a brand new perspective for bio-dots and its potential use in bioanalysis., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2016
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