1. Genetic and antigenic characterization of small ruminant lentiviruses circulating in Poland.
- Author
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Olech M, Rachid A, Croisé B, Kuźmak J, and Valas S
- Subjects
- Animals, Arthritis-Encephalitis Virus, Caprine, Cluster Analysis, Enzyme-Linked Immunosorbent Assay, Gene Products, env genetics, Gene Products, gag genetics, Heteroduplex Analysis, Lentivirus genetics, Lentivirus immunology, Molecular Sequence Data, Phylogeny, Poland, Sequence Analysis, DNA, Antigenic Variation, Genetic Variation, Goats virology, Lentivirus classification, Lentivirus isolation & purification, Sheep virology
- Abstract
Small ruminant lentivirus (SRLV) infections are widespread in Poland, but the genetic features of sheep viruses are still lacking and limited to partial gag sequences for goat viruses. In this study, segments from the gag and env genes of Polish SRLV strains screened by heteroduplex mobility assay were subjected to genetic analyses. Subtype A1 was found in both sheep and goats, while subtypes B1 and B2 were found in goats and sheep, respectively. In addition, two novel subtypes (named A12 and A13) were found in sheep. Their close phylogenetic relatedness with SRLV strains previously isolated from Polish goats indicated that these new subtypes are predominant and circulate in both species. The antigenic relationships of subtypes A12 and A13 with other SRLV subtypes were tested in an ELISA assay based on recombinant antigens carrying the immunodominant domains of structural proteins (MA, CA and SU). Antigenic cross-reactivity in the Gag epitopes was evident among genotype A subtypes and, to a lower extent, between genotypes A and B. In contrast, a subtype-specific immunoresponse was detected in the SU epitopes. These results emphasize the broad genetic and antigenic diversity of SRLV strains circulating in Europe and confirmed the need to consider all viral genotypes to choose the antigens in serological tests in order to avoid misdiagnosis in control and eradication programs., (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Published
- 2012
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