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1. [Activity of glyoxylate cycle during the culture of Rhodotorula gracilis in the presence of fatty acids and detergents].

Author

Ranaldi F, Vanni P, and Giachetti E

Subjects
Glucosephosphate Dehydrogenase metabolism, Hexokinase metabolism, In Vitro Techniques, Isocitrate Dehydrogenase metabolism, Isocitrate Lyase metabolism, Malate Synthase metabolism, Pyruvate Kinase metabolism, Rhodotorula enzymology, Detergents pharmacology, Fatty Acids pharmacology, Glyoxylates metabolism, Rhodotorula metabolism
Abstract

In this work on Rhodotorula gracilis, we studied the level of the representative enzymes of foundamental metabolic ways (HK, PK, G6PDH and IDH) and those of ICL and MS key enzymes of glyoxylate cycle with different carbone sources. The glucose appears the best source of carbone for all the tested enzymes except ICL and MS which are practically absent in the cells developed with this carbone source. In presence of acetate and ethanol we have obtained the expression of the glyoxylate cycle enzymes (ICL and MS). Moreover we obtained the operativity of the glyoxylate cycle in the lipid-rich cells or cells developped on neutral detergents or anionics similar to the fatty acids.

Published
1996

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