1. LSP1, a responsive protein from Meyerozyma guilliermondii, elicits defence response and improves glycyrrhizic acid biosynthesis in Glycyrrhiza uralensis Fisch adventitious roots.
- Author
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Wang J, Li J, Li J, Li J, Liu S, and Gao W
- Subjects
- Chromatography, High Pressure Liquid, Gene Expression Regulation, Plant drug effects, Glycyrrhiza uralensis genetics, Host-Pathogen Interactions, Mitogen-Activated Protein Kinases metabolism, Signal Transduction drug effects, Spectrometry, Mass, Electrospray Ionization, Time Factors, Up-Regulation, Fungal Proteins metabolism, Glycyrrhiza uralensis metabolism, Glycyrrhiza uralensis microbiology, Glycyrrhizic Acid metabolism, Plant Roots metabolism, Plant Roots microbiology, Saccharomycetales metabolism
- Abstract
This research explored the effects of protein and polysaccharide in Meyerozyma guilliermondii on active compounds in Glycyrrhiza uralensis Fisch adventitious roots. In this study, a responsive protein LSP1 was purified from the Meyerozyma guilliermondii since the excellent induction. The contents of total flavonoids (3.46 mg · g
-1 ), glycyrrhizic acid (0.41 mg · g-1 ), glycyrrhetinic acid (0.41 mg · g-1 ), and polysaccharide (94.49 mg · g-1 ) in adventitious root peaked at LSP1 group, which were 1.6, 3.4, 2.4, 2.0-fold that of control, respectively. Besides, the responsive protein LSP1 significantly activated the defense signaling, mitogen-activated protein kinases and extremely up-regulated the expression of defense-related genes and functional genes involved in glycyrrhizic acid biosynthesis., (© 2017 Wiley Periodicals, Inc.)- Published
- 2017
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