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The current study investigated whether the phytoglycoprotein (75 kDa) isolated from Cudrania tricuspidata Bureau (CTB glycoprotein) has anti-allergic inflammatory potential. Bisphenol A (BPA) is well known to promote development of allergy-related immune dysfunction. This experiment evaluated the release of histamine, increased intracellular Ca(2+) level, and the activities of protein kinase C alpha (PKC alpha), transcription factors NFkappaB and cytokines (IL-4, and IFN-gamma) in BPA-treated RBL-2H3 cells. The results in this study showed that the phytoglycoprotein (75 kDa) inhibits the intracellular Ca(2+) level, translocation of PKC alpha from cytosol to membrane and the translocation of NF-kappaB in cells. We also found that the phytoglycoprotein (75 kDa) inhibits the release of histamine, and the expressions of cytokines (IL-4 and IFN-gamma) in the presence of BPA in RBL-2H3 cells. The results obtained from these experiments indicated that the phytoglycoprotein (75 kDa) inhibits release of histamine and expressions of IL-4, and IFN-gamma via down regulations of Ca(2+)/PKCalpha and NF-kappaB on the stage of degranulation induced by BPA. From point of view, we speculated that the phytoglycoprotein (75 kDa) is one of natural compounds that can block allergic-inflammation caused by BPA.

The present study was performed to investigate the anti-inflammatory potential of 115 kDa glycoprotein isolated from Zanthoxylum piperitum DC leaves (ZPDC glycoprotein) in primary cultured mouse thymocytes. To determine whether the ZPDC glycoprotein has inhibitory capacity against inflammation in vitro, we evaluated the activities of inflammation-related factors such as phosphorylations of p38 mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinase (ERK) 1/2, and the activities of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) in 12-O-tetradecanoylphorbol 13-acetata (PMA, 50 nM)-treated mouse thymocytes. Our results showed that the ZPDC glycoprotein (200 mug/ml) has a suppressive effect on the expression of MAPK (ERK1/2 and p38 MAPK), on mRNA expression of pro-inflammatory cytokines (TNF-alpha and IL-1beta), and on protein expression of pro-inflammatory proteins (iNOS and COX-2). We speculate that the ZPDC glycoprotein is an example of a natural compound that blocks pro-inflammatory signal transduction pathways.

The current study investigated whether the phytoglycoprotein (75 kDa) isolated from Cudrania tricuspidata Bureau (CTB glycoprotein) has anti-allergic inflammatory potential. Bisphenol A (BPA) is well known to promote development of allergy-related immune dysfunction. This experiment evaluated the release of histamine, increased intracellular Ca2+ level, and the activities of protein kinase C α (PKC α), transcription factors NFκB and cytokines (IL-4, and IFN-γ) in BPA-treated RBL-2H3 cells. The results in this study showed that the phytoglycoprotein (75 kDa) inhibits the intracellular Ca2+ level, translocation of PKC α from cytosol to membrane and the translocation of NF-κB in cells. We also found that the phytoglycoprotein (75 kDa) inhibits the release of histamine, and the expressions of cytokines (IL-4 and IFN-γ) in the presence of BPA in RBL-2H3 cells. The results obtained from these experiments indicated that the phytoglycoprotein (75 kDa) inhibits release of histamine and expressions of IL-4, and IFN-γ via down regulations of Ca2+/PKCα and NF-κB on the stage of degranulation induced by BPA. From point of view, we speculated that the phytoglycoprotein (75 kDa) is one of natural compounds that can block allergic-inflammation caused by BPA. [ABSTRACT FROM AUTHOR]

The article presents a study to assess the anti-inflammatory potential of 115 kDa glycoprotein separated from Zanthoxylum piperitum DC leaves (ZPDC glycoprotein) in primary cultured mouse thymocytes. The study concludes that production of the intracellular reactive oxygen species (ROS) is inhibited by ZPDC glycoprotein. It asserts that ZPDC glycoprotein has anti-inflammatory potentials and is useful as health food supplement and as oriental medicine for healing inflammation.