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In the present work, a facile and green synthesis approach for the production of monodispersed, small-sized (2.9 ± 0.7 nm) and stable silver nanoparticles (AgNPs) using soluble soybean polysaccharide (SSPS) was reported. SSPS was used as the reducing and stabilizing agent. The obtained SSPS-stabilized AgNPs (SA) were characterized by UV-vis absorption spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, energy dispersive X-ray spectroscopy and transmission electron microscopy. The antimicrobial activity of the SA colloidal dispersion (SACD) was evaluated based on the growth kinetics of bacteria E. coli and S. aureus. Afterwards, the colloidal dispersion was applied as a coating material to Kraft paper. The SACD-coated Kraft paper exhibited excellent antimicrobial activity against above bacteria strains and P. aeruginosa. The effects of SACD coating on surface wettability, barrier property and microstructure of the Kraft paper were also studied. The results suggested that the SSPS-stabilized AgNPs have great potential in antibacterial applications., Competing Interests: Declaration of competing interest The authors declare no conflict of interest., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Allergen genes of the peanut and soybean were selected as model targets. Four hairpin DNA probes, H1, H2, H3, H4 were designed. Cy3-labeled H1 and H2 were used to detect peanut DNA, while FAM-labeled H3 and H4 were used to detect soybean DNA. Graphene oxide (GO) was used as the adsorption material for capturing the hairpin probes, and as a selective fluorescence quencher to reduce the background signal. To develop an allergen gene detection system with a GO-based paper chip format, we integrated the hybridization chain reaction (HCR) with fluorescence resonance energy transfer (FRET) in our design. The results showed that in the absence of peanut DNA (T P ) and soybean DNA (T S ), the detection probes attached to the GO surface, which quenched their fluorescence. In the presence of T P or T S , however, complementary probe binding to the targets initiated HCR, producing long double-stranded DNA products that could not be absorbed onto the GO surface. Hence, a strong red or green fluorescent signal was generated. The detection limit for both peanut and soybean DNA was 1 nM using this method, indicating the high sensitivity of our approach. This method also exhibited good specificity and a single chip could be used to simultaneously detect two different targets., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Plant proteins are used - for the first time - in this work as bulk binders for cellulosic fibers in paper composites. Soy bean protein and wheat gluten were denatured by two methods, namely by: urea+NaOH and by urea+NaOH+acrylamide. Addition of increased amounts of the denatured proteins resulted in a significant increase in all paper strength properties. Soy protein led, in addition, to a remarkable enhancement in opacity. The use of proteins increased kaolin retention in the paper composites, while keeping the paper strength higher than the blank protein-free paper. The results show that plant proteins are favorable than synthetic adhesives; because they are biodegradable and do not cause troubles in paper recycling i.e. they are environmentally friendly., ((c) 2010 Elsevier B.V. All rights reserved.)

A stilbene dye (Direct Yellow 11) and a methine dye, Basazol 46L, recalcitrant to common chemical bleaches, were treated with horseradish and soybean peroxidases. Both enzymes were effective at chromophore removal. When compared to laccase in combination with a mediator (ABTS), soybean peroxidase was more effective at oxidative dye removal, especially for the methine dye.

Recent studies have implicated soybeans as one of the major food allergens in children. Serum from eight patients with atopic dermatitis and positive double-blind, placebo-controlled food challenges to soy were used to investigate allergen-specific IgE and IgG antibody to soy protein and its fractions. ELISA and immunoblotting techniques were used for this purpose. A crude soy extract, as well as the 7S, 11S, and whey fractions, were isolated and purified for the in vitro studies from commercial defatted soy flakes. ELISA results demonstrated a statistically significant increased IgE and IgG response to the crude soy extract compared to that of normal control subjects. There was also an increase in IgE specific for 7S and IgG specific for 11S in the atopic group. The immunoblots reveal that IgE and IgG are present in varying amounts to multiple fractions of the soy protein. These results indicate that IgE and IgG specific to crude soy are elevated in patients with positive double-blind, placebo-controlled food challenges to soy but that no one fraction is clearly more antigenic.