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Your search keyword '"Konishi, Atsushi"' showing total 2 results
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2 results on '"Konishi, Atsushi"'

1. Effects of Organic Solvents on the Reverse Transcription Reaction Catalyzed by Reverse Transcriptases from Avian Myeloblastosis Virus and Moloney Murine Leukemia Virus.

Author

Yasukawa, Kiyoshi, Konishi, Atsushi, and Inouye, Kuniyo

Subjects
*DIMETHYL sulfoxide, *ORGANIC solvents, *GLYCERIN, *REVERSE transcriptase, *MOUSE leukemia viruses, *GENE expression, *POLYMERASE chain reaction, *ORGANIC compounds
Abstract

The article presents a study which investigates the impact of organic solvents such as glycerol and dimethyl sulfoxide (DMSO) formamide on the reverse transcription (RT) reaction catalyzed through reverse transcriptases from moloney murine leukemia virus (MMLV) and avian myeloblastosis virus (AMV). The study expressed the MMLV RT gene under the T7 promoter in Escherichia coli. Result shows that the use of organic chemicals improves yields and specificity in polymerase chain reaction (PCR).

Published
2010
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2. Comparison of the Thermal Stabilities of the αβ Heterodimer and the α Subunit of Avian Myeloblastosis Virus Reverse Transcriptase.

Author

KONISHI, Atsushi, NEMOTO, Daisuke, YASUKAWA, Kiyoshi, and INOUYE, Kuniyo

Subjects
*VIRAL physiology, *REVERSE transcriptase, *STABILITY (Mechanics), *POTASSIUM phosphates, *GLYCERIN
Abstract

The article explores the role of 63-kDa α subunit and 95-kDa β interaction in avian myeoblastosis virus reverse transcriptase (AMV RT) stability. It states that AMV RT αβ purified from AMV is dissolved in potassium phosphate, glycerol and Triton. It concludes that interaction of AMV RT α and β subunits is important to AMV RT stability.

Published
2011
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