1. Accumulation of the coumarin scopolin under abiotic stress conditions is mediated by the Arabidopsis thaliana THO/TREX complex.
- Author
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Döll S, Kuhlmann M, Rutten T, Mette MF, Scharfenberg S, Petridis A, Berreth DC, and Mock HP
- Subjects
- Arabidopsis Proteins genetics, Argonaute Proteins genetics, Argonaute Proteins metabolism, Coumarins analysis, Glucosides analysis, Glucosides genetics, Microscopy, Fluorescence, Multiprotein Complexes genetics, Multiprotein Complexes metabolism, Mutation, Osmotic Pressure, Plant Leaves genetics, Plant Leaves metabolism, Plant Roots genetics, Plant Roots metabolism, RNA Interference, RNA Precursors metabolism, RNA-Dependent RNA Polymerase genetics, RNA-Dependent RNA Polymerase metabolism, Sucrose metabolism, Temperature, Arabidopsis physiology, Arabidopsis Proteins metabolism, Coumarins metabolism, Glucosides metabolism, Stress, Physiological physiology
- Abstract
Secondary metabolites are involved in the plant stress response. Among these are scopolin and its active form scopoletin, which are coumarin derivatives associated with reactive oxygen species scavenging and pathogen defence. Here we show that scopolin accumulation can be induced in the root by osmotic stress and in the leaf by low-temperature stress in Arabidopsis thaliana. A genetic screen for altered scopolin levels in A. thaliana revealed a mutant compromised in scopolin accumulation in response to stress; the lesion was present in a homologue of THO1 coding for a subunit of the THO/TREX complex. The THO/TREX complex contributes to RNA silencing, supposedly by trafficking precursors of small RNAs. Mutants defective in THO, AGO1, SDS3 and RDR6 were impaired with respect to scopolin accumulation in response to stress, suggesting a mechanism based on RNA silencing such as the trans-acting small interfering RNA pathway, which requires THO/TREX function., (© 2017 The Authors The Plant Journal © 2017 John Wiley & Sons Ltd.)
- Published
- 2018
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