1. Vitamin E prevents steroid-induced osteonecrosis in rabbits.
- Author
-
Kuribayashi M, Fujioka M, Takahashi KA, Arai Y, Ishida M, Goto T, and Kubo T
- Subjects
- Animals, Disease Models, Animal, Femur Head Necrosis chemically induced, Glucocorticoids administration & dosage, Male, Methylprednisolone administration & dosage, Osteonecrosis chemically induced, Osteonecrosis pathology, Rabbits, Vitamins administration & dosage, Femur Head Necrosis prevention & control, Glucocorticoids adverse effects, Methylprednisolone adverse effects, Osteonecrosis prevention & control, alpha-Tocopherol administration & dosage
- Abstract
Background and Purpose: Prevention of osteonecrosis after corticosteroid administration would be important. We examined the potential of vitamin E (alpha-tocopherol) to reduce the incidence of corticosteroid-induced osteonecrosis in an animal model., Methods: Japanese white rabbits were divided into 2 groups; the control group was fed a normal diet and the experimental group was fed alpha-tocopherol-supplemented diet in which alpha-tocopherol (600 mg/kg diet) was added to the normal diet. To induce osteonecrosis, high-dose methylprednisolone acetate (MPSL) (20 mg/kg body weight) was injected once into the right gluteus medius muscle of all rabbits. 4 weeks after the injection of MPSL, the presence or absence of osteonecrosis of bilateral femurs was examined histopathologically. The tocopherol/cholesterol ratios were calculated. The plasma levels of thiobarbituric acid-reactive substances (TBARS) were measured., Results: Alpha-tocopherol-supplemented diet reduced the incidence of osteonecrosis, which developed in 14 of 20 rabbits in the control group and 5 of 21 rabbits in the experimental group (p = 0.004). The tocopherol/cholesterol ratio was higher in the experimental group than in the control group after the alpha-tocopherol administration. The plasma TBARS level was lower in the experimental group than in the control group at 4 weeks after the MPSL administration., Interpretation: Our findings may offer a new approach for the prevention of corticosteroid-induced osteonecrosis.
- Published
- 2010
- Full Text
- View/download PDF