1. Detection of α-Thalassemia in China by Using Multiplex Ligation-Dependent Probe Amplification.
- Author
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Liu, Jing-Zhong, Han, Han, Schouten, Jan P., Wang, Li-Rong, Fan, Xin-Ping, Duarte, Helena B., Zhu, Chun-Jiang, Cai, Ren, Xiao, Bai, and Wang, Qing-Tao
- Subjects
THALASSEMIA ,LIGATURE (Surgery) ,GLOBIN genes ,CHROMOSOMES ,POLYMERASE chain reaction - Abstract
The multiplex ligation-dependent probe amplification (MLPA) method was used to analyze 118 DNA samples from 90 α-thalassemia (α-thal) patients and 28 normal persons from Southern China, where the main causes of α-thal are three large deletions (-α3.7, -α4.2, and - -SEA) and two point mutations in the α-globin gene cluster on chromosome 16. The results, detected by the P140B HBA kit, were in complete concordance with the results detected by multiplex polmymerase chain reaction (m-PCR) and real-time PCR. The advantages and limitations of the techniques are discussed. We concluded that MLPA was a rapid and reliable method to determine the cause of both deletional and nondeletional α-thal in China. [ABSTRACT FROM AUTHOR]
- Published
- 2008
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