Search

A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium, strain ChDC PVNT-B20 T , was isolated from the human subgingival dental plaque of a gingivitis lesion. Phylogenetic analysis based on the 16S ribosomal RNA gene (16S rDNA) showed that the strain belonged to the genus Paenibacillus. BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593 T (97.7% similarity) and Paenibacillus macerans ATCC 8244 T (97.6% similarity). Draft genome of strain ChDC PVNT-B20 T was composed of 8,112,407 bp. The DNA G+C content of the strain was 51.3 mol%. Average nucleotide identity values between strain ChDC PVNT-B20 T and P. faecis DSM 23593 T or P. macerans ATCC 8244 T were 75.71% and 91.5%, respectively. Genome-to-genome distance values between strain ChDC PVNT-B20 T and P. faecis DSM 23593 T or P. macerans ATCC 8244 T were 21.6% (19.3-24.0%) and 44.9% (42.3-47.4%), respectively. Major cellular fatty acids of strain ChDC PVNT-B20 T were anteiso-C 15:0 (43.4%), C 16:0 (16.6%), iso-C 16:0 (14.4%), and anteiso-C 17:0 (12.4%). The sole respiratory quinone of the strain was menaqinone-7. Major polar lipids of the strain were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), and one unidentified glycolipid (GL). Minor polar lipids were one unidentified aminolipid (AL), one unidentified phospholipid (PL), and three unidentified lipids (L1-L3). Based on these results, strain ChDC PVNT-B20 T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus oralis sp. nov. is proposed. Type strain is ChDC PVNT-B20 T (= KCOM 3021 T  = JCM 33462  T ).

A novel Gram-stain-negative, motile, and facultative anaerobic coccus, strain ChDC F240 T was isolated from human subgingival dental plaque of a gingivitis lesion. The phylogenetic analysis based on the 16S ribosomal RNA gene (16S rDNA) sequence showed that the strain belonged to the genus Lautropia. 16S rDNA of strain ChDC F240 T had the highest similarity to that of Lautropia mirabilis ATCC 51599 T (98.8%). Major cellular fatty acids of strain ChDC F240 T were C 16:0 (43.9%) and C 16:1ω6C /C 16:1ω7C (38.1%). Draft genome of the strain was 3,834,139 bp in length and the G+C content was 65.0 mol%. Average nucleotide identity and genome-to-genome distance values between strain ChDC F240 T and L. mirabilis ATCC 51599  T were 81.99% and 28.50% (26.1-30.9%), respectively. These results reveal that strain ChDC F240 T is a novel species within the genus Lautropia, for which the name Lautropia dentalis sp. nov. is proposed; type strain is ChDC F240 T (= KCOM 2505 T  = JCM 33297 T ).

A novel Gram-stain-positive, obligately anaerobic, spore-forming rod, designated strain ChDC B114 T , was isolated from a human dental plaque of a gingivitis lesion. The strain was characterized by polyphasic taxonomic analysis to identify it at the species level. The 16S ribosomal RNA gene (16S rDNA) sequence analysis revealed that the strain belongs to the genus Lachnoanaerobaculum. The percent similarity of the 16S rDNA of the strain was closest to the homologous gene sequence of Lachnoanaerobaculum orale N1 T (98.5%) and Lachnoanaerobaculum saburreum CCUG 28089 T (97.6%). The major fatty acids of strain ChDC B114 T were C 16:0 (30.7%), C 14:0 (17.7%), iso-C 19:0 (14.9%), and C 17:0 2OH (12.0%). The draft genome of strain ChDC B114 T was 3,097,953 bp in length. The G+C content of the strain was 35.9 mol %. Average nucleotide identity values between strain ChDC B114 T and L. orale N1 T and L. saburreum CCUG 28089 T were 83.2% and 82.0%, respectively. Genome-to-genome distance values between strain ChDC B114 T and L. orale N1 T and L. saburreum CCUG 28089 T were 26.8% (24.5-29.3%) and 26.30% (24.0-28.8%), respectively. Based on these results, strain ChDC B114 T (= KCOM 2030 T  = JCM 33452 T ) should be classified as a novel species of genus Lachnoanaerobaculum, for which the name Lachnoanaerobaculum gingivalis sp. nov. is proposed.

N.L. gen. masc. n. I vincentii i , of Vincent, referring to H. Vincent who studied the organism originally isolated from Vincent's angina and necrotizing ulcerative gingivitis. L. neut. n. I animal i ( I gen. animalis i ), an animal; L. gen. n. I animalis i , of an animal. Correction to: Current Microbiology (2017) 74:1137-1147 https://doi.org/10.1007/s00284-017-12... The original and previous erratum versions of this article contained errors in the description of novel species. [Extracted from the article]