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Abstract: Two selected amino acids (l-glutamine and l-proline) and polyethylene glycol (PEG) were evaluated for their effects on maturation and germination of somatic embryos in guava (Psidium guajava L.) cv. Banarasi local. Among the treatments of two amino acids (l-glutamine and l-proline) and PEG tested, 0.87mM l-proline was most effective for maturation of somatic embryos. As compared to control, supplementation of PEG (1.0% or 2.0%) in maturation media showed significantly better maturation (increased maturation frequency). Lower stage somatic embryos showed prematuration germination with development of abnormal plantlets and only mature somatic embryos produced morphologically normal plantlets. [Copyright &y& Elsevier]

Abstract: Present study demonstrates the effect of sucrose and ABA on germination of encapsulated somatic embryos of guava (Psidium guajava L.). Sucrose and ABA at different concentrations were also evaluated for their effects on maturation and germination of somatic embryos. Mature somatic embryos developed on MS medium containing high concentration of sucrose (10%) or ABA (1.0mgl−1) showed inhibition in germination if they continued to be in same medium for 4 weeks. With increasing concentrations of sucrose (3–9%) or ABA (0.01–1.0mgl−1) in medium, percent germination of encapsulated somatic embryos decreased significantly. Encapsulated somatic embryos after storage on MS medium supplemented with 9% sucrose or 1mgl−1 ABA for different duration (0–60 days) germinated when they were transferred to medium containing 3% sucrose. About 20.8% and 37.5% encapsulated somatic embryos germinated after storage on ABA (1mgl−1) or sucrose (9%) for 60 days, respectively. Temporarily suppression in germination of encapsulated somatic embryos by high concentration of sucrose or ABA may be important for short-term conservation of elite genotype of guava. [Copyright &y& Elsevier]