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Impact events that create complex craters excavate mid- to lower-crustal rocks, offering a unique perspective on the interior composition and internal dynamics of planetary bodies. On the Yucatán Peninsula, Mexico, the surface geology mainly consists of ∼3 km thick sedimentary rocks, with a lack of exposure of crystalline basement in many areas. Consequently, current understanding of the Yucatán subsurface is largely based on impact ejecta and drill cores recovered from the 180–200-km-diameter Chicxulub impact structure. In this study, we present the first apatite and titanite U Pb ages for pre-impact dacitic, doleritic, and felsitic magmatic dikes preserved in Chicxulub's peak ring sampled during the 2016 IODP-ICDP Expedition 364. Dating yielded two age groups, with Carboniferous dacites (328–318 Ma) and a felsite (330± 9 Ma) overlapping in age with most of the granitoid basement sampled in the Expedition 364 drill core, as well as Jurassic dolerites (169–159 Ma) and a felsite (158 ± 19 Ma) that represent the first in situ sampling of Jurassic-age magmatic intrusions for the Yucatán Peninsula. Further investigation of the Nd, Sr, and Hf isotopic compositions of these pre-impact lithologies and impact melt rocks from the peak ring structure suggest that dolerites generally contributed up to ∼10 vol% of the Chicxulub impact melt rock sampled in the peak ring. This percentage implies that the dolerites comprised a large part of the Yucatán subsurface by volume, representing a hitherto unsampled pervasive Jurassic magmatic phase. We interpret this magmatic phase to be related to the opening of the Gulf of Mexico, representing the first physical sampling of lithologies associated with the southern extension of the opening of the Gulf of Mexico and likely constraining its onset to the Late Middle Jurassic. • The Chicxulub impact structure excavates Jurassic dolerites • First in situ Jurassic crystalline lithologies discovered on the Yucatán Peninsula • Impact melt rock composition reveals these dolerites to be a pervasive magmatic phase • These dolerites are associated with the opening of the Gulf of Mexico [ABSTRACT FROM AUTHOR]